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The map label for this gene is nuoI

Identifier: 85374152

GI number: 85374152

Start: 1376055

End: 1376543

Strand: Reverse

Name: nuoI

Synonym: ELI_06625

Alternate gene names: 85374152

Gene position: 1376543-1376055 (Counterclockwise)

Preceding gene: 85374153

Following gene: 85374151

Centisome position: 45.1

GC content: 60.12

Gene sequence:

>489_bases
ATGACCACCGCAACCCAACTCCTCAAATCGTTCACCTTGTGGGAATTCCTCAAGGCGCATGCGCTGACCTTGAAGTATTT
CTTCAAGCCCAAGGCGACGATCAACTACCCGTTCGAAAAGAACCCGCTGAGCCCGCGCTTCCGCGGCGAGCATGCGCTGC
GTCGCTATCCCAATGGCGAGGAACGCTGCATCGCCTGCAAGCTGTGTGAGGCGGTCTGCCCGGCGCAGGCGATCACGATC
GAGAGCGAACCGCGCGACGACGGCAGCCGCCGCACCACCCGCTACGATATCGATATGACAAAGTGCATCTATTGCGGCTT
CTGCCAGGAAGCGTGCCCGGTGGACGCCATCGTCGAGGGTCCGAACTTCGAATATTCGACCGAAACCCGCGAGGAGCTGC
TCTACGACAAGGCAAAGCTGCTCGCCAATGGTGACAAGTGGGAGCGGGCGATCGCCGCAAACCTTGAAGCCGATGCGCCG
TATCGCTAA

Upstream 100 bases:

>100_bases
ACGACCAGCTGATGCGGCTCGGATGGAAGGTCTTCCTGCCGATGAGCCTGCTCTTCGTTTTCCTTATCAGCGGCTATCTG
ATGGCCACGGGGCACTACGG

Downstream 100 bases:

>100_bases
CCGCGCGCCAGACGAAACCGGGGCACCATGATACAAGCATTCGCCTTTTACCTGTTCGCAGCGCTGATGATCGCCAGCGG
CGTGCTGACGATCATGAGCC

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MTTATQLLKSFTLWEFLKAHALTLKYFFKPKATINYPFEKNPLSPRFRGEHALRRYPNGEERCIACKLCEAVCPAQAITI
ESEPRDDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEYSTETREELLYDKAKLLANGDKWERAIAANLEADAP
YR

Sequences:

>Translated_162_residues
MTTATQLLKSFTLWEFLKAHALTLKYFFKPKATINYPFEKNPLSPRFRGEHALRRYPNGEERCIACKLCEAVCPAQAITI
ESEPRDDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEYSTETREELLYDKAKLLANGDKWERAIAANLEADAP
YR
>Mature_161_residues
TTATQLLKSFTLWEFLKAHALTLKYFFKPKATINYPFEKNPLSPRFRGEHALRRYPNGEERCIACKLCEAVCPAQAITIE
SEPRDDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEYSTETREELLYDKAKLLANGDKWERAIAANLEADAPY
R

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=163, Percent_Identity=76.0736196319018, Blast_Score=260, Evalue=4e-70,
Organism=Escherichia coli, GI1788617, Length=147, Percent_Identity=38.0952380952381, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1789075, Length=127, Percent_Identity=29.1338582677165, Blast_Score=62, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17555194, Length=155, Percent_Identity=73.5483870967742, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI17864306, Length=150, Percent_Identity=80.6666666666667, Blast_Score=258, Evalue=9e-70,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI_ERYLH (Q2NA74)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458214.1
- HSSP:   Q9I6D2
- ProteinModelPortal:   Q2NA74
- SMR:   Q2NA74
- STRING:   Q2NA74
- GeneID:   3870389
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_06625
- NMPDR:   fig|314225.3.peg.1726
- eggNOG:   COG1143
- HOGENOM:   HBG724359
- OMA:   LQADHLY
- PhylomeDB:   Q2NA74
- ProtClustDB:   PRK05888
- BioCyc:   ELIT314225:ELI_06625-MONOMER
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 18603; Mature: 18472

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTATQLLKSFTLWEFLKAHALTLKYFFKPKATINYPFEKNPLSPRFRGEHALRRYPNGE
CCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCCCCCCCCCCCCHHHHCCCCCC
ERCIACKLCEAVCPAQAITIESEPRDDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG
HHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCEEECC
PNFEYSTETREELLYDKAKLLANGDKWERAIAANLEADAPYR
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TTATQLLKSFTLWEFLKAHALTLKYFFKPKATINYPFEKNPLSPRFRGEHALRRYPNGE
CHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCCCCCCCCCCCCHHHHCCCCCC
ERCIACKLCEAVCPAQAITIESEPRDDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEG
HHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCEEECC
PNFEYSTETREELLYDKAKLLANGDKWERAIAANLEADAPYR
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA