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The map label for this gene is dus [H]

Identifier: 85374084

GI number: 85374084

Start: 1307890

End: 1308906

Strand: Reverse

Name: dus [H]

Synonym: ELI_06285

Alternate gene names: 85374084

Gene position: 1308906-1307890 (Counterclockwise)

Preceding gene: 85374088

Following gene: 85374083

Centisome position: 42.88

GC content: 63.42

Gene sequence:

>1017_bases
ATGAGCGAACTTCCTTCCCCTCCGGCCCTGAAGCCGATCCAGGTCGGCCCGGTCACGATCGACACGCCGGTCGTGCTTGC
GCCGATGACGGGCGTGACCGACCTGCCGTTCCGCACGCTCGTTCGTCGCTTTGGCTCGGGCCTCAACGTCACCGAGATGA
TCGCCAGCGAAGCGGCCATTCGCGAGACGCGCCAGTCGGTGCAGAAGGCCGCGTGGGACAAGACCGAAGACCCTGTCTCG
ATGCAGCTCGTCGGCTGCGACCCGGTCTCGATGGGAGAGGCCGCCAAGCTGCAGGAAGACAACGGCGCAGCGATCATCGA
CATCAATTTCGGCTGTCCGGTGCGCAAGGTGGTCGGCCAGCTCGCGGGCAGCGCCTTGATGCGCGAAGTCCCGCTTGCGA
CAAGGCTGATGGAAGCGACCGTCAATGCTGTCGACGTTCCGGTCACGGTCAAGATGCGGATGGGCTGGGATCACGCCAGC
CTCAATGCGCCCGAGCTCGCGCGCATCGCTGAGGATCTGGGCGTCCAGATGATCACCGTTCACGGTCGCACCCGCAACCA
GATGTACAAGGGAAGCGCCGACTGGTCCTTCATTCGCAAGGTCAAGGACGCGGTCTCGATCCCCGTCATCGTGAACGGCG
ATATCTGCGGGATCGAGGACGCGGCCAACGCGCTGGAACAGTCTGGCGCCGACGGGTTGATGATCGGGCGCGGAGCCTAT
GGCAAGCCGTGGCTGCTCGCGCAGGTCATGCATTGGTGGAAGACCGGCGACAGGCTCGAAAGTCCGGATTTCGACTTGCA
ATACGAAACGCTGGTCGAACATTACGGGCGCATGCTCGATCACTATGGCCGTGAAGTCGGCACCAAGATCGCCCGCAAGC
ATCTGGGCTGGTACACCAAGGGCATGCACGGCTCGGCCGAATTCCGCAATTTCGTCAATTTCATCGACGATCCCGACCAG
GTTCTGGGCGAGATCGAGCGGTTCTACACGCCCTTCCTGCAGCGGCGGGCGGCGTGA

Upstream 100 bases:

>100_bases
GGGGACTCGGCGTGGCCATGTGCCATGCGCGCTACCGCCTTGCGCCTGCGCTCGCAATCCACTAGATGCTGCCCAAATTT
TAGGCATATCGCAGCGCAGC

Downstream 100 bases:

>100_bases
GCGACGCGCCCGCGCGGCCCGATCCGCGCGCGCAAATCGCCGGGATGGTCTTTGCCGTGTTGCTGATCGACCCGGACGCC
GTCATCGCCGAAGTGAACCC

Product: tRNA-dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSELPSPPALKPIQVGPVTIDTPVVLAPMTGVTDLPFRTLVRRFGSGLNVTEMIASEAAIRETRQSVQKAAWDKTEDPVS
MQLVGCDPVSMGEAAKLQEDNGAAIIDINFGCPVRKVVGQLAGSALMREVPLATRLMEATVNAVDVPVTVKMRMGWDHAS
LNAPELARIAEDLGVQMITVHGRTRNQMYKGSADWSFIRKVKDAVSIPVIVNGDICGIEDAANALEQSGADGLMIGRGAY
GKPWLLAQVMHWWKTGDRLESPDFDLQYETLVEHYGRMLDHYGREVGTKIARKHLGWYTKGMHGSAEFRNFVNFIDDPDQ
VLGEIERFYTPFLQRRAA

Sequences:

>Translated_338_residues
MSELPSPPALKPIQVGPVTIDTPVVLAPMTGVTDLPFRTLVRRFGSGLNVTEMIASEAAIRETRQSVQKAAWDKTEDPVS
MQLVGCDPVSMGEAAKLQEDNGAAIIDINFGCPVRKVVGQLAGSALMREVPLATRLMEATVNAVDVPVTVKMRMGWDHAS
LNAPELARIAEDLGVQMITVHGRTRNQMYKGSADWSFIRKVKDAVSIPVIVNGDICGIEDAANALEQSGADGLMIGRGAY
GKPWLLAQVMHWWKTGDRLESPDFDLQYETLVEHYGRMLDHYGREVGTKIARKHLGWYTKGMHGSAEFRNFVNFIDDPDQ
VLGEIERFYTPFLQRRAA
>Mature_337_residues
SELPSPPALKPIQVGPVTIDTPVVLAPMTGVTDLPFRTLVRRFGSGLNVTEMIASEAAIRETRQSVQKAAWDKTEDPVSM
QLVGCDPVSMGEAAKLQEDNGAAIIDINFGCPVRKVVGQLAGSALMREVPLATRLMEATVNAVDVPVTVKMRMGWDHASL
NAPELARIAEDLGVQMITVHGRTRNQMYKGSADWSFIRKVKDAVSIPVIVNGDICGIEDAANALEQSGADGLMIGRGAYG
KPWLLAQVMHWWKTGDRLESPDFDLQYETLVEHYGRMLDHYGREVGTKIARKHLGWYTKGMHGSAEFRNFVNFIDDPDQV
LGEIERFYTPFLQRRAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788462, Length=292, Percent_Identity=32.8767123287671, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI239788483, Length=282, Percent_Identity=33.3333333333333, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI31742496, Length=229, Percent_Identity=32.3144104803493, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI40807366, Length=232, Percent_Identity=31.8965517241379, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI8923374, Length=243, Percent_Identity=27.5720164609054, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1789660, Length=320, Percent_Identity=40, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1788462, Length=235, Percent_Identity=32.7659574468085, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI145693211, Length=237, Percent_Identity=29.535864978903, Blast_Score=92, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17543114, Length=293, Percent_Identity=31.3993174061433, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25144369, Length=286, Percent_Identity=29.020979020979, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17507177, Length=266, Percent_Identity=30.0751879699248, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323560, Length=229, Percent_Identity=33.1877729257642, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323433, Length=316, Percent_Identity=26.5822784810127, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6323437, Length=231, Percent_Identity=28.1385281385281, Blast_Score=89, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24585320, Length=287, Percent_Identity=32.0557491289199, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24580595, Length=232, Percent_Identity=32.3275862068966, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI19920448, Length=232, Percent_Identity=32.3275862068966, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI19921524, Length=226, Percent_Identity=30.9734513274336, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI45549423, Length=234, Percent_Identity=26.9230769230769, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37277; Mature: 37146

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELPSPPALKPIQVGPVTIDTPVVLAPMTGVTDLPFRTLVRRFGSGLNVTEMIASEAAI
CCCCCCCCCCCCEEECCEEECCCEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
RETRQSVQKAAWDKTEDPVSMQLVGCDPVSMGEAAKLQEDNGAAIIDINFGCPVRKVVGQ
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHEECCCCCEEEEEECCCCHHHHHHH
LAGSALMREVPLATRLMEATVNAVDVPVTVKMRMGWDHASLNAPELARIAEDLGVQMITV
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCEEEEEE
HGRTRNQMYKGSADWSFIRKVKDAVSIPVIVNGDICGIEDAANALEQSGADGLMIGRGAY
ECCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCCEEECCCCC
GKPWLLAQVMHWWKTGDRLESPDFDLQYETLVEHYGRMLDHYGREVGTKIARKHLGWYTK
CCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHC
GMHGSAEFRNFVNFIDDPDQVLGEIERFYTPFLQRRAA
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SELPSPPALKPIQVGPVTIDTPVVLAPMTGVTDLPFRTLVRRFGSGLNVTEMIASEAAI
CCCCCCCCCCCEEECCEEECCCEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
RETRQSVQKAAWDKTEDPVSMQLVGCDPVSMGEAAKLQEDNGAAIIDINFGCPVRKVVGQ
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHEECCCCCEEEEEECCCCHHHHHHH
LAGSALMREVPLATRLMEATVNAVDVPVTVKMRMGWDHASLNAPELARIAEDLGVQMITV
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCEEEEEE
HGRTRNQMYKGSADWSFIRKVKDAVSIPVIVNGDICGIEDAANALEQSGADGLMIGRGAY
ECCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCCEEECCCCC
GKPWLLAQVMHWWKTGDRLESPDFDLQYETLVEHYGRMLDHYGREVGTKIARKHLGWYTK
CCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHC
GMHGSAEFRNFVNFIDDPDQVLGEIERFYTPFLQRRAA
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7553451 [H]