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The map label for this gene is ntrX [H]

Identifier: 85374080

GI number: 85374080

Start: 1301712

End: 1303088

Strand: Reverse

Name: ntrX [H]

Synonym: ELI_06265

Alternate gene names: 85374080

Gene position: 1303088-1301712 (Counterclockwise)

Preceding gene: 85374081

Following gene: 85374079

Centisome position: 42.69

GC content: 63.91

Gene sequence:

>1377_bases
ATGGCGCTCGATATCCTGGTCGTCGACGATGAACGCGATATTCGCGAACTGGTGTCCGGCGTGCTCAGCGACGAAGGCTA
TGAATGCCGGACCGCAGGCGACAGCAACAGCGCGCTGCAAATGTTCGATGAGCGTCGGCCTAGCGTCGTGCTGCTCGACG
TATGGCTGCACGGAAGCGCGATGGACGGGTTGGAAGTGCTGGACGTCATCAAGGAGCGCGAGCCCGACCTGCCGGTGATC
ATCTTCTCCGGACACGGCAATATCGATACCGCCGTGTCCGCTGTGGGCCGCGGGGCGATGGACTTCATCGAAAAGCCGTT
CGAAGCCTCCCGCCTGTTGCATCTTGTGTCGCGGGCAACCGAAACGCAGCGGTTGCGGCGCGAAAACGTCCGCTTGCGCC
AGGGCTTCACCGGCGGAGAGGAGTTCACCGGAAACTCGTCTGCGATCAACCAGGTGCGCGCGACGCTCAAACGGGTTGCC
AATACCGGGAGCCGGGTGCTTATCTCGGGTCCGGCAGGGGCGGGCAAGGAAGTGGCCGCGCGGCTGTTGCATAGCTGGAG
CCCTCGGGCCGATCATCCCTTCGTGATCGTCAACTCCGCGCGCATCACCCCCGAACGTTTCGAGCAGGAGCTGTTCGGCG
AGGAAGCCGAAGGAAAGCTGGTGCGGCCGGGCCTGCTGGAACTGGCCGACGGGGGGACGCTCTATCTTGACGAAGTGGCC
GACATGCCGCTGTCGACGCAGGCGCGGATCCTGCGCGTCCTGACCGATCAGAGCTTCGTGCGCGTCGGCGGAAATCGTCA
GATCGGGGTCGACGTTCGCGTGGTATCCTCGACCGCGCGCGACCTGCAGGTCGAAATCGACGAGAAGAACTTCCGCGAGG
ACCTGTTCTACCGGCTCAACGTGGTGCCGGTCACCATCCCTTCGCTATCGGAGCGGCGCGACGACGTGCCCGCGCTGGCG
GAGCATTTCTTCACGCGCTACGCGGCGGAACAGGGCATCCCGGCGCCGGAAATCAGCGAGGAAGCGATGGCGGCGCTCCA
GGCCTATGACTGGCCGGGGAACGTGCGCCAGTTGCGCAATGTCGTGGAGCGGACCGTTATCCTGTCGCCGCGCGACCGGA
TGGAGGAAGTCCAGCCCGACATGCTCCCCAGCGAAGTAACCTCGGGCAAGCTCGGGGGAGGGACGGGCATGACCAATCTC
ATGGGCGTGCCGTTGCGAGAAGCGCGCGAAAGCTTCGAGCGGGAATATCTCAGCATCCAGATTCGCCGCTTCTCGGGCAA
TATTTCAAAGACCGCGAGCTTTATCGGGATGGAGCGCTCGGCGCTCCACCGCAAGCTGAAGATTCTCGGCATGACCGAGC
GCAAGAACGGCGATTGA

Upstream 100 bases:

>100_bases
TGCGTCACGCTGCGCTTCGCGCGCCGTCCCTCCGTGCCGGAAAGCGAGGCCGCCGAATGATGCGCACATATCCCGAGCTT
GAAAATGAGGAGACGCTGTA

Downstream 100 bases:

>100_bases
AACGCACCGTCACCAACGGCTCACAAATGATTGCAAAATATCGCATTGAATTGATGCGATCTTGCGCACATAATTAAAGC
CAGATTTCGGACGGGCGTCT

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MALDILVVDDERDIRELVSGVLSDEGYECRTAGDSNSALQMFDERRPSVVLLDVWLHGSAMDGLEVLDVIKEREPDLPVI
IFSGHGNIDTAVSAVGRGAMDFIEKPFEASRLLHLVSRATETQRLRRENVRLRQGFTGGEEFTGNSSAINQVRATLKRVA
NTGSRVLISGPAGAGKEVAARLLHSWSPRADHPFVIVNSARITPERFEQELFGEEAEGKLVRPGLLELADGGTLYLDEVA
DMPLSTQARILRVLTDQSFVRVGGNRQIGVDVRVVSSTARDLQVEIDEKNFREDLFYRLNVVPVTIPSLSERRDDVPALA
EHFFTRYAAEQGIPAPEISEEAMAALQAYDWPGNVRQLRNVVERTVILSPRDRMEEVQPDMLPSEVTSGKLGGGTGMTNL
MGVPLREARESFEREYLSIQIRRFSGNISKTASFIGMERSALHRKLKILGMTERKNGD

Sequences:

>Translated_458_residues
MALDILVVDDERDIRELVSGVLSDEGYECRTAGDSNSALQMFDERRPSVVLLDVWLHGSAMDGLEVLDVIKEREPDLPVI
IFSGHGNIDTAVSAVGRGAMDFIEKPFEASRLLHLVSRATETQRLRRENVRLRQGFTGGEEFTGNSSAINQVRATLKRVA
NTGSRVLISGPAGAGKEVAARLLHSWSPRADHPFVIVNSARITPERFEQELFGEEAEGKLVRPGLLELADGGTLYLDEVA
DMPLSTQARILRVLTDQSFVRVGGNRQIGVDVRVVSSTARDLQVEIDEKNFREDLFYRLNVVPVTIPSLSERRDDVPALA
EHFFTRYAAEQGIPAPEISEEAMAALQAYDWPGNVRQLRNVVERTVILSPRDRMEEVQPDMLPSEVTSGKLGGGTGMTNL
MGVPLREARESFEREYLSIQIRRFSGNISKTASFIGMERSALHRKLKILGMTERKNGD
>Mature_457_residues
ALDILVVDDERDIRELVSGVLSDEGYECRTAGDSNSALQMFDERRPSVVLLDVWLHGSAMDGLEVLDVIKEREPDLPVII
FSGHGNIDTAVSAVGRGAMDFIEKPFEASRLLHLVSRATETQRLRRENVRLRQGFTGGEEFTGNSSAINQVRATLKRVAN
TGSRVLISGPAGAGKEVAARLLHSWSPRADHPFVIVNSARITPERFEQELFGEEAEGKLVRPGLLELADGGTLYLDEVAD
MPLSTQARILRVLTDQSFVRVGGNRQIGVDVRVVSSTARDLQVEIDEKNFREDLFYRLNVVPVTIPSLSERRDDVPALAE
HFFTRYAAEQGIPAPEISEEAMAALQAYDWPGNVRQLRNVVERTVILSPRDRMEEVQPDMLPSEVTSGKLGGGTGMTNLM
GVPLREARESFEREYLSIQIRRFSGNISKTASFIGMERSALHRKLKILGMTERKNGD

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=457, Percent_Identity=36.1050328227571, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI1790299, Length=475, Percent_Identity=38.3157894736842, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1790437, Length=450, Percent_Identity=35.3333333333333, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1788905, Length=448, Percent_Identity=35.0446428571429, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI87082117, Length=347, Percent_Identity=36.5994236311239, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1787583, Length=323, Percent_Identity=34.984520123839, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI87082152, Length=328, Percent_Identity=37.1951219512195, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1789087, Length=271, Percent_Identity=39.4833948339483, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI1786524, Length=324, Percent_Identity=36.7283950617284, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1789233, Length=322, Percent_Identity=33.2298136645963, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI87081872, Length=326, Percent_Identity=33.7423312883436, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI87081858, Length=300, Percent_Identity=27.6666666666667, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789828, Length=227, Percent_Identity=31.2775330396476, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1786599, Length=116, Percent_Identity=37.0689655172414, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50851; Mature: 50720

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDILVVDDERDIRELVSGVLSDEGYECRTAGDSNSALQMFDERRPSVVLLDVWLHGSA
CEEEEEEECCCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHCCCCCEEEEEEEECCCC
MDGLEVLDVIKEREPDLPVIIFSGHGNIDTAVSAVGRGAMDFIEKPFEASRLLHLVSRAT
CCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHH
ETQRLRRENVRLRQGFTGGEEFTGNSSAINQVRATLKRVANTGSRVLISGPAGAGKEVAA
HHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
RLLHSWSPRADHPFVIVNSARITPERFEQELFGEEAEGKLVRPGLLELADGGTLYLDEVA
HHHHCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCEECCCHHEECCCCEEEEHHHH
DMPLSTQARILRVLTDQSFVRVGGNRQIGVDVRVVSSTARDLQVEIDEKNFREDLFYRLN
CCCCCHHHHHHHHHHCCCCEEECCCEEEEEEEEEECCCCCCEEEEECCHHHHHHHHEEEE
VVPVTIPSLSERRDDVPALAEHFFTRYAAEQGIPAPEISEEAMAALQAYDWPGNVRQLRN
EEEEECCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHH
VVERTVILSPRDRMEEVQPDMLPSEVTSGKLGGGTGMTNLMGVPLREARESFEREYLSIQ
HHHHHEEECCHHHHHHCCCCCCCHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHEEE
IRRFSGNISKTASFIGMERSALHRKLKILGMTERKNGD
EEEECCCHHHHHHHHCCHHHHHHHHHHHEEECCCCCCC
>Mature Secondary Structure 
ALDILVVDDERDIRELVSGVLSDEGYECRTAGDSNSALQMFDERRPSVVLLDVWLHGSA
EEEEEEECCCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHCCCCCEEEEEEEECCCC
MDGLEVLDVIKEREPDLPVIIFSGHGNIDTAVSAVGRGAMDFIEKPFEASRLLHLVSRAT
CCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHH
ETQRLRRENVRLRQGFTGGEEFTGNSSAINQVRATLKRVANTGSRVLISGPAGAGKEVAA
HHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
RLLHSWSPRADHPFVIVNSARITPERFEQELFGEEAEGKLVRPGLLELADGGTLYLDEVA
HHHHCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCEECCCHHEECCCCEEEEHHHH
DMPLSTQARILRVLTDQSFVRVGGNRQIGVDVRVVSSTARDLQVEIDEKNFREDLFYRLN
CCCCCHHHHHHHHHHCCCCEEECCCEEEEEEEEEECCCCCCEEEEECCHHHHHHHHEEEE
VVPVTIPSLSERRDDVPALAEHFFTRYAAEQGIPAPEISEEAMAALQAYDWPGNVRQLRN
EEEEECCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHH
VVERTVILSPRDRMEEVQPDMLPSEVTSGKLGGGTGMTNLMGVPLREARESFEREYLSIQ
HHHHHEEECCHHHHHHCCCCCCCHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHEEE
IRRFSGNISKTASFIGMERSALHRKLKILGMTERKNGD
EEEECCCHHHHHHHHCCHHHHHHHHHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]