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The map label for this gene is queA

Identifier: 85373945

GI number: 85373945

Start: 1158147

End: 1159190

Strand: Direct

Name: queA

Synonym: ELI_05590

Alternate gene names: 85373945

Gene position: 1158147-1159190 (Clockwise)

Preceding gene: 85373944

Following gene: 85373946

Centisome position: 37.94

GC content: 62.84

Gene sequence:

>1044_bases
ATGCGTGTAGACCTGTTCGATTTCGAACTCCCTCAGGAGCTGATTGCGCTGCGCCCGGCGGTGCCGCGCGATTCAGCGCG
TCTGCTGGTGGCCGCAGGCGCTGGCGCGATCGACGACCGTATCGTGCGCGACCTGCCGCAGCTGCTGATGCCGGGCGATG
TCCTCGTCTTCAACGATACCAGGGTCATTCCGGCACAACTGACCGGCAAGCGGGGCGAAGCCACCGTAGGCGTGACGTTG
CACAAGCGGCTCGACCTGCGCCGCTGGCAGGCCTTCGTCCGCAATGCCAAGCGTCTGGCGCCGGGTGATACGGTCGATTT
CGGCAACGATGTCAGCGCCGATGTCGAGGATCGCGCCGACGATGGCAGCTTCACCTTCGCCTTTCGTGGTGATGAGCCGG
TCGAGGTGTTGCTCGACCGTGCGGGCACCATGCCGCTTCCACCCTATATCGCCGGCAAGCGTGCGACGGATGAGCGCGAC
CGCGACGACTACCAGACGATGTTCGCACGGGAAGATGGCGCGGTTGCCGCGCCGACGGCGGCATTGCATTTCACATCCGA
GCTGATCGCGGCACTCGATGAGCGCGGCGTCGCGCGCCAAACGCTGACGCTCCATGTGGGCGCTGGCACATTCCTCCCGG
TAAAGGCGGACGATACCGACGATCACCGGATGCATTCGGAATGGGGTCGGATCGAACACGACACCGCCGATCGCCTTAAC
GCCGCCAAGTCACGCGGCGGCAGGATCATCGCGGTCGGAACGACCAGCCTACGCCTGCTGGAAAGCGCGACGGGCGAGGA
TGGTGTCATCCGGACCTTTGCAGGCGATACGGACATCTTCATCACGCCGGGCTACCGCTTTCGTGCGGTGGATGGCCTGA
TGACCAATTTCCATTTGCCCAAATCCACGCTGATGATGCTGGTCAGCGCGCTGATGGGACGCGAACGGATGATGGATATC
TACGCCCACGCCATTGCGCAGAGATATCGTTTCTATTCCTATGGCGATTCTTCGCTGTTGATCCCAGAGGGGAACACCAA
GTAG

Upstream 100 bases:

>100_bases
CCTCGGCCGGCCCAGCCCGAGCGCGAGATCACGCTCGACGAGCTGAGCGCTCCGATCGAGGGGTGACGCAGCGCGCGAGG
GGCGCTAACCGCGCGCTTCG

Downstream 100 bases:

>100_bases
CGGTTTGAAACCAATTCAGGAGCCGCACATGAGTGAGCCCATTCTCGACATCCAGGGCCTGACCAAGATCTATCCCGGCG
GCCTGAAGGCATTGGACGAT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MRVDLFDFELPQELIALRPAVPRDSARLLVAAGAGAIDDRIVRDLPQLLMPGDVLVFNDTRVIPAQLTGKRGEATVGVTL
HKRLDLRRWQAFVRNAKRLAPGDTVDFGNDVSADVEDRADDGSFTFAFRGDEPVEVLLDRAGTMPLPPYIAGKRATDERD
RDDYQTMFAREDGAVAAPTAALHFTSELIAALDERGVARQTLTLHVGAGTFLPVKADDTDDHRMHSEWGRIEHDTADRLN
AAKSRGGRIIAVGTTSLRLLESATGEDGVIRTFAGDTDIFITPGYRFRAVDGLMTNFHLPKSTLMMLVSALMGRERMMDI
YAHAIAQRYRFYSYGDSSLLIPEGNTK

Sequences:

>Translated_347_residues
MRVDLFDFELPQELIALRPAVPRDSARLLVAAGAGAIDDRIVRDLPQLLMPGDVLVFNDTRVIPAQLTGKRGEATVGVTL
HKRLDLRRWQAFVRNAKRLAPGDTVDFGNDVSADVEDRADDGSFTFAFRGDEPVEVLLDRAGTMPLPPYIAGKRATDERD
RDDYQTMFAREDGAVAAPTAALHFTSELIAALDERGVARQTLTLHVGAGTFLPVKADDTDDHRMHSEWGRIEHDTADRLN
AAKSRGGRIIAVGTTSLRLLESATGEDGVIRTFAGDTDIFITPGYRFRAVDGLMTNFHLPKSTLMMLVSALMGRERMMDI
YAHAIAQRYRFYSYGDSSLLIPEGNTK
>Mature_347_residues
MRVDLFDFELPQELIALRPAVPRDSARLLVAAGAGAIDDRIVRDLPQLLMPGDVLVFNDTRVIPAQLTGKRGEATVGVTL
HKRLDLRRWQAFVRNAKRLAPGDTVDFGNDVSADVEDRADDGSFTFAFRGDEPVEVLLDRAGTMPLPPYIAGKRATDERD
RDDYQTMFAREDGAVAAPTAALHFTSELIAALDERGVARQTLTLHVGAGTFLPVKADDTDDHRMHSEWGRIEHDTADRLN
AAKSRGGRIIAVGTTSLRLLESATGEDGVIRTFAGDTDIFITPGYRFRAVDGLMTNFHLPKSTLMMLVSALMGRERMMDI
YAHAIAQRYRFYSYGDSSLLIPEGNTK

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=345, Percent_Identity=46.3768115942029, Blast_Score=288, Evalue=4e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_ERYLH (Q2NAT1)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458007.1
- HSSP:   O32054
- ProteinModelPortal:   Q2NAT1
- SMR:   Q2NAT1
- STRING:   Q2NAT1
- GeneID:   3870669
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_05590
- NMPDR:   fig|314225.3.peg.1946
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- PhylomeDB:   Q2NAT1
- ProtClustDB:   PRK00147
- BioCyc:   ELIT314225:ELI_05590-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38303; Mature: 38303

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDLFDFELPQELIALRPAVPRDSARLLVAAGAGAIDDRIVRDLPQLLMPGDVLVFNDT
CEEEEECCCCCHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCC
RVIPAQLTGKRGEATVGVTLHKRLDLRRWQAFVRNAKRLAPGDTVDFGNDVSADVEDRAD
EEEEHEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
DGSFTFAFRGDEPVEVLLDRAGTMPLPPYIAGKRATDERDRDDYQTMFAREDGAVAAPTA
CCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEECCHH
ALHFTSELIAALDERGVARQTLTLHVGAGTFLPVKADDTDDHRMHSEWGRIEHDTADRLN
HHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEEECCCCCCHHHHHHHCCCCCCHHHHHH
AAKSRGGRIIAVGTTSLRLLESATGEDGVIRTFAGDTDIFITPGYRFRAVDGLMTNFHLP
HHHHCCCEEEEECCCHHHHHHHCCCCCCEEEEECCCCCEEECCCCEEEEHHHHHHHCCCC
KSTLMMLVSALMGRERMMDIYAHAIAQRYRFYSYGDSSLLIPEGNTK
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHEEEECCCEEEECCCCCC
>Mature Secondary Structure
MRVDLFDFELPQELIALRPAVPRDSARLLVAAGAGAIDDRIVRDLPQLLMPGDVLVFNDT
CEEEEECCCCCHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCC
RVIPAQLTGKRGEATVGVTLHKRLDLRRWQAFVRNAKRLAPGDTVDFGNDVSADVEDRAD
EEEEHEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
DGSFTFAFRGDEPVEVLLDRAGTMPLPPYIAGKRATDERDRDDYQTMFAREDGAVAAPTA
CCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEECCHH
ALHFTSELIAALDERGVARQTLTLHVGAGTFLPVKADDTDDHRMHSEWGRIEHDTADRLN
HHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEEECCCCCCHHHHHHHCCCCCCHHHHHH
AAKSRGGRIIAVGTTSLRLLESATGEDGVIRTFAGDTDIFITPGYRFRAVDGLMTNFHLP
HHHHCCCEEEEECCCHHHHHHHCCCCCCEEEEECCCCCEEECCCCEEEEHHHHHHHCCCC
KSTLMMLVSALMGRERMMDIYAHAIAQRYRFYSYGDSSLLIPEGNTK
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHEEEECCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA