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The map label for this gene is leuA [H]

Identifier: 85373920

GI number: 85373920

Start: 1136890

End: 1138569

Strand: Reverse

Name: leuA [H]

Synonym: ELI_05465

Alternate gene names: 85373920

Gene position: 1138569-1136890 (Counterclockwise)

Preceding gene: 85373925

Following gene: 85373919

Centisome position: 37.3

GC content: 62.86

Gene sequence:

>1680_bases
ATGCGCAATATGTTGCGCGACCCCGCGCGAAAATATGCGGCCTTTCCGCAAATCGACCTGCCCGACCGGCAATGGCCGAG
CCGGACTATAACCGCCCCGCCGCGCTGGCTCAGCACCGATCTGCGCGACGGCAATCAGTCCATCATCGATCCCATGGATG
CAGTGAAGAAGAACCGCTTTTTCGACCTGCTGGTGGAGGTAGGGATTAAGGAAATCGAAGTCGGCTTTCCCAGCGCAGGG
GCAACCGAGTTCGATTTCATCCAGGGATTGGTGCGCTCGGGACGCATCCCCGATGATGTGACCGTACAAGTGCTCACGCA
GAGCCGCGAAGAGCTGATCCGCACCAGCTTCGAAAGTCTGGCAGGCGCGCGTGCGGCGATCGTCCATCTCTACAACGCAG
TCAGCCCAGCATGGCGCGAGATCGTGTTCGGTATGACGACCGAAGAAGTGATCGGCGTTGCTGTGAAAGGCGCACAGGTG
CTTGCAGACGAAGCAGACAAGCGGCCCGACACCGATTGGCAGTTCGAATATTCACCTGAGACTTTCTCCACCGCCGAGAT
CGATTTCAGCATCGAAATCTGCGCGGCAGTGATGGAAGTTCTCCAGCCCACCCCGGACAGGCCGATCATCCTCAACCTGC
CTGCCACGGTAGAGGCGGCAACGCCCAACATCTACGCCGACCAGATCGAGTATTTCTGCAAGCATCTGCCGAACCGCGAG
AGCGCGGTGATCAGCCTGCATACGCACAACGATCGCGGCACGGGCGTGGCGGCAGCCGAACTCGGCCTGATGGCGGGCGC
AGACAGGGTCGAAGGCTGCCTGTTCGGCAATGGCGAGCGCACGGGCAATTGCTGCCTCGTGACCATGGCGCTCAACATGT
ACACACAGGGCCTCGATCCGGAGCTCGACTTTTCAGACATCGACCGCGTGATCGAGACGGTCGAGTACTGCAACCAGCTC
CCCGTCCACCAGCGTCATCCCTATGGCGGCGAGCTGGTCTACACCGCTTTTTCGGGCAGCCACCAGGACGCAATCAAGAA
AGGCTTCGCCGCGCACGAGCGACAGAACGACGAGCGCTGGCGCGTGCCCTACCTGCCGATCGATCCGGCCGACCTGGGTC
GCACTTACGAAGCCGTCATCCGCGTCAACTCGCAAAGCGGCAAAGGCGGCTTCGCCTGGGTGCTCGAGCAGGACCAAGGC
CTCAGATTGCCCAAGAAGATGCAGGCCGACTTCTCGCCCCACGTGCAGGCCATGGCCGACAAACTCGGGCGCGAGCTCAA
TGCCCAAGACATCTGGCAGACCTTCCGCAGCGCCTACCATGTGCAGACGCCCGACAAGCGCTTCCAGCTCGTCAGCTACG
AGGAAAGTCGCGCCAGCGACGGGACGCGCCTGTTCACCGGCACGATCGCGGTGGACGGCGAAGAGCGGAGCGTCAGCGGC
CGCGGCAACGGCCTGATCTCCAGCGTGATGGCGACCCTGCGCGATGCATTCGACGTGCAGCTGGAGGTGGTGGATTATAC
CGAGCATGCGCTCGGTGCCGGCACCGACGCCAATGCGGCGGCCTATCTCGAATGCCGGACGCCCGATGGTCGCACCATCT
GGGGCTGCGGGATCGACGAGGACGTCGCCACTGCCTCGGTGCGCGCGGTACTGAGCGCGGCCAACAGCGCCGTGACCTAG

Upstream 100 bases:

>100_bases
ATCCGGCGTGCGGTAACGGCGCGCCACGCGCCCAAGGGTATCCGCCACCTTCGACCAGACGACCGACACACCGGAAATCA
TGACCCAGCCCGAGCCAAGA

Downstream 100 bases:

>100_bases
GCGAAGACAGGAGAGAGGCAAATGCAGGTTCGGACGTTTCTTGCTGGGCTGGCGATAGCAGTCGGACCTGCCATCCTTGC
GCTCCCACTCGCGGCGCATC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MRNMLRDPARKYAAFPQIDLPDRQWPSRTITAPPRWLSTDLRDGNQSIIDPMDAVKKNRFFDLLVEVGIKEIEVGFPSAG
ATEFDFIQGLVRSGRIPDDVTVQVLTQSREELIRTSFESLAGARAAIVHLYNAVSPAWREIVFGMTTEEVIGVAVKGAQV
LADEADKRPDTDWQFEYSPETFSTAEIDFSIEICAAVMEVLQPTPDRPIILNLPATVEAATPNIYADQIEYFCKHLPNRE
SAVISLHTHNDRGTGVAAAELGLMAGADRVEGCLFGNGERTGNCCLVTMALNMYTQGLDPELDFSDIDRVIETVEYCNQL
PVHQRHPYGGELVYTAFSGSHQDAIKKGFAAHERQNDERWRVPYLPIDPADLGRTYEAVIRVNSQSGKGGFAWVLEQDQG
LRLPKKMQADFSPHVQAMADKLGRELNAQDIWQTFRSAYHVQTPDKRFQLVSYEESRASDGTRLFTGTIAVDGEERSVSG
RGNGLISSVMATLRDAFDVQLEVVDYTEHALGAGTDANAAAYLECRTPDGRTIWGCGIDEDVATASVRAVLSAANSAVT

Sequences:

>Translated_559_residues
MRNMLRDPARKYAAFPQIDLPDRQWPSRTITAPPRWLSTDLRDGNQSIIDPMDAVKKNRFFDLLVEVGIKEIEVGFPSAG
ATEFDFIQGLVRSGRIPDDVTVQVLTQSREELIRTSFESLAGARAAIVHLYNAVSPAWREIVFGMTTEEVIGVAVKGAQV
LADEADKRPDTDWQFEYSPETFSTAEIDFSIEICAAVMEVLQPTPDRPIILNLPATVEAATPNIYADQIEYFCKHLPNRE
SAVISLHTHNDRGTGVAAAELGLMAGADRVEGCLFGNGERTGNCCLVTMALNMYTQGLDPELDFSDIDRVIETVEYCNQL
PVHQRHPYGGELVYTAFSGSHQDAIKKGFAAHERQNDERWRVPYLPIDPADLGRTYEAVIRVNSQSGKGGFAWVLEQDQG
LRLPKKMQADFSPHVQAMADKLGRELNAQDIWQTFRSAYHVQTPDKRFQLVSYEESRASDGTRLFTGTIAVDGEERSVSG
RGNGLISSVMATLRDAFDVQLEVVDYTEHALGAGTDANAAAYLECRTPDGRTIWGCGIDEDVATASVRAVLSAANSAVT
>Mature_559_residues
MRNMLRDPARKYAAFPQIDLPDRQWPSRTITAPPRWLSTDLRDGNQSIIDPMDAVKKNRFFDLLVEVGIKEIEVGFPSAG
ATEFDFIQGLVRSGRIPDDVTVQVLTQSREELIRTSFESLAGARAAIVHLYNAVSPAWREIVFGMTTEEVIGVAVKGAQV
LADEADKRPDTDWQFEYSPETFSTAEIDFSIEICAAVMEVLQPTPDRPIILNLPATVEAATPNIYADQIEYFCKHLPNRE
SAVISLHTHNDRGTGVAAAELGLMAGADRVEGCLFGNGERTGNCCLVTMALNMYTQGLDPELDFSDIDRVIETVEYCNQL
PVHQRHPYGGELVYTAFSGSHQDAIKKGFAAHERQNDERWRVPYLPIDPADLGRTYEAVIRVNSQSGKGGFAWVLEQDQG
LRLPKKMQADFSPHVQAMADKLGRELNAQDIWQTFRSAYHVQTPDKRFQLVSYEESRASDGTRLFTGTIAVDGEERSVSG
RGNGLISSVMATLRDAFDVQLEVVDYTEHALGAGTDANAAAYLECRTPDGRTIWGCGIDEDVATASVRAVLSAANSAVT

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=536, Percent_Identity=25.5597014925373, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324225, Length=571, Percent_Identity=51.8388791593695, Blast_Score=601, Evalue=1e-172,
Organism=Saccharomyces cerevisiae, GI6324682, Length=570, Percent_Identity=50, Blast_Score=589, Evalue=1e-169,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 61713; Mature: 61713

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNMLRDPARKYAAFPQIDLPDRQWPSRTITAPPRWLSTDLRDGNQSIIDPMDAVKKNRF
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHCCHHHHHHHHHH
FDLLVEVGIKEIEVGFPSAGATEFDFIQGLVRSGRIPDDVTVQVLTQSREELIRTSFESL
HHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AGARAAIVHLYNAVSPAWREIVFGMTTEEVIGVAVKGAQVLADEADKRPDTDWQFEYSPE
HHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEECCC
TFSTAEIDFSIEICAAVMEVLQPTPDRPIILNLPATVEAATPNIYADQIEYFCKHLPNRE
CCCEEEEEEHHHHHHHHHHHHCCCCCCCEEEECCCEEECCCCCHHHHHHHHHHHHCCCCC
SAVISLHTHNDRGTGVAAAELGLMAGADRVEGCLFGNGERTGNCCLVTMALNMYTQGLDP
CEEEEEEECCCCCCCCHHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEEEHHHHHCCCCC
ELDFSDIDRVIETVEYCNQLPVHQRHPYGGELVYTAFSGSHQDAIKKGFAAHERQNDERW
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCE
RVPYLPIDPADLGRTYEAVIRVNSQSGKGGFAWVLEQDQGLRLPKKMQADFSPHVQAMAD
ECCCCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHCCCCHHHHHHHH
KLGRELNAQDIWQTFRSAYHVQTPDKRFQLVSYEESRASDGTRLFTGTIAVDGEERSVSG
HHCCCCCHHHHHHHHHHHEECCCCCHHEEEEEECHHCCCCCCEEEEEEEEECCCCCCCCC
RGNGLISSVMATLRDAFDVQLEVVDYTEHALGAGTDANAAAYLECRTPDGRTIWGCGIDE
CCCHHHHHHHHHHHHHHCEEEEEEECHHHHHCCCCCCCCEEEEEEECCCCCEEEECCCCH
DVATASVRAVLSAANSAVT
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRNMLRDPARKYAAFPQIDLPDRQWPSRTITAPPRWLSTDLRDGNQSIIDPMDAVKKNRF
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHCCHHHHHHHHHH
FDLLVEVGIKEIEVGFPSAGATEFDFIQGLVRSGRIPDDVTVQVLTQSREELIRTSFESL
HHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AGARAAIVHLYNAVSPAWREIVFGMTTEEVIGVAVKGAQVLADEADKRPDTDWQFEYSPE
HHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEECCC
TFSTAEIDFSIEICAAVMEVLQPTPDRPIILNLPATVEAATPNIYADQIEYFCKHLPNRE
CCCEEEEEEHHHHHHHHHHHHCCCCCCCEEEECCCEEECCCCCHHHHHHHHHHHHCCCCC
SAVISLHTHNDRGTGVAAAELGLMAGADRVEGCLFGNGERTGNCCLVTMALNMYTQGLDP
CEEEEEEECCCCCCCCHHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEEEHHHHHCCCCC
ELDFSDIDRVIETVEYCNQLPVHQRHPYGGELVYTAFSGSHQDAIKKGFAAHERQNDERW
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCE
RVPYLPIDPADLGRTYEAVIRVNSQSGKGGFAWVLEQDQGLRLPKKMQADFSPHVQAMAD
ECCCCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHCCCCHHHHHHHH
KLGRELNAQDIWQTFRSAYHVQTPDKRFQLVSYEESRASDGTRLFTGTIAVDGEERSVSG
HHCCCCCHHHHHHHHHHHEECCCCCHHEEEEEECHHCCCCCCEEEEEEEEECCCCCCCCC
RGNGLISSVMATLRDAFDVQLEVVDYTEHALGAGTDANAAAYLECRTPDGRTIWGCGIDE
CCCHHHHHHHHHHHHHHCEEEEEEECHHHHHCCCCCCCCEEEEEEECCCCCEEEECCCCH
DVATASVRAVLSAANSAVT
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA