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The map label for this gene is thiE

Identifier: 85373876

GI number: 85373876

Start: 1084679

End: 1085320

Strand: Direct

Name: thiE

Synonym: ELI_05245

Alternate gene names: 85373876

Gene position: 1084679-1085320 (Clockwise)

Preceding gene: 85373874

Following gene: 85373877

Centisome position: 35.54

GC content: 62.77

Gene sequence:

>642_bases
ATGGCTGAAACAGAAATTCCGACGCAGCTCTATTTGATCTCACCGCTCGACGTGCGTGGCGGTTTCCCGCAGCGGCTCGA
GCGGGCGCTCGAAGCGGGACGGGGGGTTGTCACCGCTTTCCAGTTCCGCGTGAAAGGCATCGACCAGCACGAAGCCGCGC
GCCTGGCCGAGCCGCTCCAGGCGATCTGTGCCGCGCATGATGTCGCCTTTGTCGTCAATGACAGTATCGCTCTGGCAAAG
CGCCTGAAGGCTGACGGCGTCCATCTGGGACAGGACGACGGATCGCCCAAGGAGGCGCGTGAGCAGTTGGGGCGCGAGGC
GCAGATTGGCGTCACTTGTCATGCTTCGAGGCACCTCGCGATGGAGGCGGGTGAGGCCGGGGCGGACTACGTTGCCTTCG
GCGCATTTTTCGACAGCGCGACCAAGGACAAGGGTGACGCCGAACAGCCGACTCTCGAATTGCTCGAATGGTGGAGCCAG
GTGTTCGAAATTCCCTGCGTCGCAATCGGCGGGATTACGCCCGATAATTGTCAGCCGCTGATCGAAGCCGGGGCCGATTT
CCTGGCCGTATCCGGAGCGGTCTGGAGCGGCGATGAAAGAGCGGCAGTTCAAGCCTTTGCGGAGCAAATAGCCGCGGCGT
GA

Upstream 100 bases:

>100_bases
TAGCCGGGTTTGAGCCCGCGCGCTGCTCGGGATTTGCACTGTCCCAATCGCTGACAGATCGCCCATTGGCGCAGGCGGAG
GTCGCGGCTAAGGGGCGCGC

Downstream 100 bases:

>100_bases
GTTGAACTTTTCGAAAAGGGGCAAAATGCGCCCCGTCATTCGGGAGTTTCCTCATGCGTGTCGCCCTTCTTGCCACCCTT
TCTACCTTCGCACTTGTCGC

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MAETEIPTQLYLISPLDVRGGFPQRLERALEAGRGVVTAFQFRVKGIDQHEAARLAEPLQAICAAHDVAFVVNDSIALAK
RLKADGVHLGQDDGSPKEAREQLGREAQIGVTCHASRHLAMEAGEAGADYVAFGAFFDSATKDKGDAEQPTLELLEWWSQ
VFEIPCVAIGGITPDNCQPLIEAGADFLAVSGAVWSGDERAAVQAFAEQIAAA

Sequences:

>Translated_213_residues
MAETEIPTQLYLISPLDVRGGFPQRLERALEAGRGVVTAFQFRVKGIDQHEAARLAEPLQAICAAHDVAFVVNDSIALAK
RLKADGVHLGQDDGSPKEAREQLGREAQIGVTCHASRHLAMEAGEAGADYVAFGAFFDSATKDKGDAEQPTLELLEWWSQ
VFEIPCVAIGGITPDNCQPLIEAGADFLAVSGAVWSGDERAAVQAFAEQIAAA
>Mature_212_residues
AETEIPTQLYLISPLDVRGGFPQRLERALEAGRGVVTAFQFRVKGIDQHEAARLAEPLQAICAAHDVAFVVNDSIALAKR
LKADGVHLGQDDGSPKEAREQLGREAQIGVTCHASRHLAMEAGEAGADYVAFGAFFDSATKDKGDAEQPTLELLEWWSQV
FEIPCVAIGGITPDNCQPLIEAGADFLAVSGAVWSGDERAAVQAFAEQIAAA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=188, Percent_Identity=29.7872340425532, Blast_Score=60, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6325042, Length=187, Percent_Identity=30.4812834224599, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_ERYLH (Q2NB00)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457938.1
- ProteinModelPortal:   Q2NB00
- STRING:   Q2NB00
- GeneID:   3869146
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_05245
- NMPDR:   fig|314225.3.peg.2019
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   FIVNDSI
- ProtClustDB:   CLSK891382
- BioCyc:   ELIT314225:ELI_05245-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 22700; Mature: 22568

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETEIPTQLYLISPLDVRGGFPQRLERALEAGRGVVTAFQFRVKGIDQHEAARLAEPLQ
CCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHCCCCHHHHHHHHHHHH
AICAAHDVAFVVNDSIALAKRLKADGVHLGQDDGSPKEAREQLGREAQIGVTCHASRHLA
HHHHHHHHHEEECCHHHHHHHHHCCCCEECCCCCCHHHHHHHCCCCCCCEEEEECCCHHH
MEAGEAGADYVAFGAFFDSATKDKGDAEQPTLELLEWWSQVFEIPCVAIGGITPDNCQPL
HHCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHEECCCCCCCCHHH
IEAGADFLAVSGAVWSGDERAAVQAFAEQIAAA
HHCCCCEEEECCCEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AETEIPTQLYLISPLDVRGGFPQRLERALEAGRGVVTAFQFRVKGIDQHEAARLAEPLQ
CCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHCCCCHHHHHHHHHHHH
AICAAHDVAFVVNDSIALAKRLKADGVHLGQDDGSPKEAREQLGREAQIGVTCHASRHLA
HHHHHHHHHEEECCHHHHHHHHHCCCCEECCCCCCHHHHHHHCCCCCCCEEEEECCCHHH
MEAGEAGADYVAFGAFFDSATKDKGDAEQPTLELLEWWSQVFEIPCVAIGGITPDNCQPL
HHCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHEECCCCCCCCHHH
IEAGADFLAVSGAVWSGDERAAVQAFAEQIAAA
HHCCCCEEEECCCEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA