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The map label for this gene is 85373771

Identifier: 85373771

GI number: 85373771

Start: 975564

End: 976775

Strand: Direct

Name: 85373771

Synonym: ELI_04720

Alternate gene names: NA

Gene position: 975564-976775 (Clockwise)

Preceding gene: 85373767

Following gene: 85373772

Centisome position: 31.96

GC content: 59.08

Gene sequence:

>1212_bases
ATGTCCACAGTCCTCGTAATCGGTGCCGGCGGCGTCAGCTCGGTCTGCGTCCACAAGATGGCGTTCAATTCCGACATCTT
CACGAGCATCCACCTCGCCAGCCGCACCAAAAGCAAATGCGATGCCATCGCTGCCAGCGTGAAGGATCGCGCGGGTGTCG
ATGTGGCGACCTACGAAATCGACGCCGAAGAAGTCCCGGCCATGGTCAACCTGATCCGCAAGGTGCAGCCGAGCCTCGTG
GTCAACCTGGCGCTGCCGTATCAGGACCTGCCGATCATGGATGCATGCCTCGAAGCGGGCGTCGATTACCTCGACACCGC
CAATTACGAGCCTAAGGACGAGGCCAAGTTCGAATATAAATGGCAATGGGTTTATCACGACCGTTTCAAGGATGCAGGGC
TGATGGCGCTGCTCGGCTCGGGCTTCGATCCCGGCGTGACCAGCGTCTTCACCATGTGGCTGAAGAAGCACAAGCTAAAG
ACCATCCGCCAGCTCGACATCCTCGACTGCAATGGCGGCGATCACGGCCAGGCCTTTGCCACCAATTTCAATCCGGAAAT
CAACATCCGCGAAGTGACTGCGCCCGCGCGCCACTGGGAGAACGGCGAATTCGTCGAGACCCCCGCGATGGGCAAGAAGA
TCACCTTCGACTTCGAAGGCGTGGGCGAGAAGAACGCCTACATGATGTATCACGAGGAGCTGGAAAGCCTCGCCAAGTTC
AATCCGGAGCTGGAGCGCGCGCGCTTCTGGATGACTTTCGGCGACGAATACATCAAGCACCTGACCGTGCTGCAGAATGT
CGGCATGACTCGGATCGATCCGATCAAGTACAAGGGCCGCGAAATCATCCCGCTGCAATTCCTCGCCGCGGTGTTGCCCA
AGCCCGAAACGCTGGGCGAAACGACCAAGGGCAACACCAACATCGGCGTCATCGCGACCGGCGAAGCGCTCGACGGTTCA
GGCGAGAAGACCTTCTACATCAATAATATCTGCAGCCACGAAGCCGCCTATGAGGAAACCGGCAACCAGGCCGTCAGCTA
CACCACCGGCGTCCCGGCAATGATCGGTTCGGCCATGATGGTGACCGGCAAGTGGAAAGGCGACGGTGTCTTCAACATGG
AAGAGATGGACCCCGATCCGTTCATGGAGATGCTCAATGAGCATGGCCTGCCGTGGCAGGTGAAGGAGCTGGACGGGCCG
GTGGACTTCTAG

Upstream 100 bases:

>100_bases
GCTTCGAGCACGCCGTCGCGGGTGGGTTTTCGGATCATTTTGCCGTGATAGCGCATTCGACTAAGAGCAGCGCAAGATTC
GCATTCGAAAGGCCCCCTCC

Downstream 100 bases:

>100_bases
AGAATTTGGTTCGCGCAGAGAGCGCAGAGGAAAGGAGATCGCAGAGGTGCTGAATTGCAGCGTTGGGATTTCCTCCATCG
TTATCGAGGAAGCTATCGGT

Product: saccharopine dehydrogenase

Products: L-lysine; 2-oxoglutarate; NADH; H+

Alternate protein names: Carboxynorspermidine Dehydrogenase; Saccharopine Dehydrogenase-Like Protein; Carboxynorspermide Hydratase; Saccharopine Dehydrogenase Family Protein; Acyl Carrier Protein

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSTVLVIGAGGVSSVCVHKMAFNSDIFTSIHLASRTKSKCDAIAASVKDRAGVDVATYEIDAEEVPAMVNLIRKVQPSLV
VNLALPYQDLPIMDACLEAGVDYLDTANYEPKDEAKFEYKWQWVYHDRFKDAGLMALLGSGFDPGVTSVFTMWLKKHKLK
TIRQLDILDCNGGDHGQAFATNFNPEINIREVTAPARHWENGEFVETPAMGKKITFDFEGVGEKNAYMMYHEELESLAKF
NPELERARFWMTFGDEYIKHLTVLQNVGMTRIDPIKYKGREIIPLQFLAAVLPKPETLGETTKGNTNIGVIATGEALDGS
GEKTFYINNICSHEAAYEETGNQAVSYTTGVPAMIGSAMMVTGKWKGDGVFNMEEMDPDPFMEMLNEHGLPWQVKELDGP
VDF

Sequences:

>Translated_403_residues
MSTVLVIGAGGVSSVCVHKMAFNSDIFTSIHLASRTKSKCDAIAASVKDRAGVDVATYEIDAEEVPAMVNLIRKVQPSLV
VNLALPYQDLPIMDACLEAGVDYLDTANYEPKDEAKFEYKWQWVYHDRFKDAGLMALLGSGFDPGVTSVFTMWLKKHKLK
TIRQLDILDCNGGDHGQAFATNFNPEINIREVTAPARHWENGEFVETPAMGKKITFDFEGVGEKNAYMMYHEELESLAKF
NPELERARFWMTFGDEYIKHLTVLQNVGMTRIDPIKYKGREIIPLQFLAAVLPKPETLGETTKGNTNIGVIATGEALDGS
GEKTFYINNICSHEAAYEETGNQAVSYTTGVPAMIGSAMMVTGKWKGDGVFNMEEMDPDPFMEMLNEHGLPWQVKELDGP
VDF
>Mature_402_residues
STVLVIGAGGVSSVCVHKMAFNSDIFTSIHLASRTKSKCDAIAASVKDRAGVDVATYEIDAEEVPAMVNLIRKVQPSLVV
NLALPYQDLPIMDACLEAGVDYLDTANYEPKDEAKFEYKWQWVYHDRFKDAGLMALLGSGFDPGVTSVFTMWLKKHKLKT
IRQLDILDCNGGDHGQAFATNFNPEINIREVTAPARHWENGEFVETPAMGKKITFDFEGVGEKNAYMMYHEELESLAKFN
PELERARFWMTFGDEYIKHLTVLQNVGMTRIDPIKYKGREIIPLQFLAAVLPKPETLGETTKGNTNIGVIATGEALDGSG
EKTFYINNICSHEAAYEETGNQAVSYTTGVPAMIGSAMMVTGKWKGDGVFNMEEMDPDPFMEMLNEHGLPWQVKELDGPV
DF

Specific function: Unknown

COG id: COG1748

COG function: function code E; Saccharopine dehydrogenase and related proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.5.1.7

Molecular weight: Translated: 44809; Mature: 44677

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVLVIGAGGVSSVCVHKMAFNSDIFTSIHLASRTKSKCDAIAASVKDRAGVDVATYEI
CCEEEEEECCCHHHHHHHHHHCCCCHHEEEEHHHCCHHHHHHHHHHHHHHCCCEEEEEEE
DAEEVPAMVNLIRKVQPSLVVNLALPYQDLPIMDACLEAGVDYLDTANYEPKDEAKFEYK
CHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCCHHHCCCCCCCCCCCEEEE
WQWVYHDRFKDAGLMALLGSGFDPGVTSVFTMWLKKHKLKTIRQLDILDCNGGDHGQAFA
EEEEEECCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEE
TNFNPEINIREVTAPARHWENGEFVETPAMGKKITFDFEGVGEKNAYMMYHEELESLAKF
ECCCCCEEEEEECCCHHHCCCCCEEECCCCCCEEEEEECCCCCCCEEEEEHHHHHHHHHC
NPELERARFWMTFGDEYIKHLTVLQNVGMTRIDPIKYKGREIIPLQFLAAVLPKPETLGE
CCCHHHHEEEEHHHHHHHHHHHHHHHCCCCEECCEEECCCEECHHHHHHHHCCCCHHCCC
TTKGNTNIGVIATGEALDGSGEKTFYINNICSHEAAYEETGNQAVSYTTGVPAMIGSAMM
CCCCCCCEEEEEECCCCCCCCCEEEEECCHHCCHHHHHHCCCCEEEEECCCHHHHCCEEE
VTGKWKGDGVFNMEEMDPDPFMEMLNEHGLPWQVKELDGPVDF
EEEEECCCCEEEHHHCCCCHHHHHHHHCCCCEEEHCCCCCCCC
>Mature Secondary Structure 
STVLVIGAGGVSSVCVHKMAFNSDIFTSIHLASRTKSKCDAIAASVKDRAGVDVATYEI
CEEEEEECCCHHHHHHHHHHCCCCHHEEEEHHHCCHHHHHHHHHHHHHHCCCEEEEEEE
DAEEVPAMVNLIRKVQPSLVVNLALPYQDLPIMDACLEAGVDYLDTANYEPKDEAKFEYK
CHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCCHHHCCCCCCCCCCCEEEE
WQWVYHDRFKDAGLMALLGSGFDPGVTSVFTMWLKKHKLKTIRQLDILDCNGGDHGQAFA
EEEEEECCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEE
TNFNPEINIREVTAPARHWENGEFVETPAMGKKITFDFEGVGEKNAYMMYHEELESLAKF
ECCCCCEEEEEECCCHHHCCCCCEEECCCCCCEEEEEECCCCCCCEEEEEHHHHHHHHHC
NPELERARFWMTFGDEYIKHLTVLQNVGMTRIDPIKYKGREIIPLQFLAAVLPKPETLGE
CCCHHHHEEEEHHHHHHHHHHHHHHHCCCCEECCEEECCCEECHHHHHHHHCCCCHHCCC
TTKGNTNIGVIATGEALDGSGEKTFYINNICSHEAAYEETGNQAVSYTTGVPAMIGSAMM
CCCCCCCEEEEEECCCCCCCCCEEEEECCHHCCHHHHHHCCCCEEEEECCCHHHHCCEEE
VTGKWKGDGVFNMEEMDPDPFMEMLNEHGLPWQVKELDGPVDF
EEEEECCCCEEEHHHCCCCHHHHHHHHCCCCEEEHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N6-(L-1,3-dicarboxypropyl)-L-lysine; NAD+; H2O

Specific reaction: N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O = L-lysine + 2-oxoglutarate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA