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The map label for this gene is nccB [H]

Identifier: 85373715

GI number: 85373715

Start: 918897

End: 920045

Strand: Reverse

Name: nccB [H]

Synonym: ELI_04440

Alternate gene names: 85373715

Gene position: 920045-918897 (Counterclockwise)

Preceding gene: 85373716

Following gene: 85373714

Centisome position: 30.14

GC content: 62.23

Gene sequence:

>1149_bases
ATGAACCGTAAGCGTTTGGCCGTACTGATCGGCCTGATCATTCTTGTTTTTGCGGCTGCTGTAATGCTGTGGCCGGACAA
CGGCGCAGAACCGCACAGCGAAGAGGAAACCGAAGAAAGCGATCTCCCGGAAGGACTGGTCCTACTCGGCGAAGAGCAGA
TTCGAAGCTCCGAAATCATGGTCGAGACCGTTGGTGAGGGCTCGGCGGTAGAGCTAGTGTTCCCAGCCACAATCGCCGCT
AGCCCCACAGGCAGCGCGCGGATCGATGCGCGAGCGGCGGGCGTGGTGCGGAGCGTTCAAAAAACGCTGGGTGATTACGT
CCGGCAGGGTGAAACCATTGCGCGTATCGAAAGTGCGGAAGCGGCAGCTTTGGCATCGCAATTGAGCGCGGCGCGCGCGC
GCGTCAACGAATTGTCAGCCGCCTATGAGCGCGAACGCCGGCTTTTCGAAGCCAATGTTACGGCGCGGCAGGATCTCGAA
GCGGTGCGCGCCAATCTCCAGGTCGCCCAGTCAGAACTGTCCCGCGCGCAGGCAGCGGTTGCGGCAGCGGGCGTGAGTGG
CGACGGTCGGTCTTTGGCGGTTACCAGCCCTCTTGCAGGACGGGTAACGGCGGCGCCTATTGTCCTTGGTTCGTTTGTCG
ATGCGGGAGAGGAACTCTACCAAATCGTGAATCCTAATGCGATGCAGATCGAAGTCGCGCTCCCCTCTGCAGAAGCGAGC
CGTATTCAGCCAGGCGATGAAGCCACGCTGGAACTTGCAGAAAACCGCGAGATCGGAGCGCGGGTCCGCTCCGTCACGCC
ATCGCTCGATCCTGAGAGCCGCAGCGCGACGGCAGTCCTTTCGCTCACGCGGTCCATCCCCGGTCTTCAACCAGGTGCCT
TCCTGCAAGCGCGCATCCGACCTTCGGGCGAGGTCGACACGGGCCGCATGTCGGTGCCCGAAAGCGCGGTGCAGTCACTC
GACGGCCAGGAAGTGGTTTTCGTGCGCACGCGCCGCGGCTTCCAGACCCGTCCGGTGGTGACCGGCGCACGTTCCGCAGG
CCGCGTGGTGATCGAATCCGGTCTCGAAGCGAACTGGCGGATCGCAACCGAGAATGCCTTCCTGCTCAAAGCCGAACTCG
AAAAAGAGGAAGCCGAACATGGACACTGA

Upstream 100 bases:

>100_bases
CCCGCGATGCAATCCGAGAAGCCTTGATCCAAGCCCAGGAAGATCGCGGTCGCCTGGCGGCGCAGTTGATCTGGCTTGGC
GCCCAATGAGGAAAATACCC

Downstream 100 bases:

>100_bases
TCGCATGCAGGAAACGGAGCACTCGCACCGCCACGGCCTGATCGGCATGATCCTCGACATGGCCGTGCGTTTTCGCTGGG
CCATGGTCGTTTTGACGATC

Product: metal ion efflux membrane fusion protein family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MNRKRLAVLIGLIILVFAAAVMLWPDNGAEPHSEEETEESDLPEGLVLLGEEQIRSSEIMVETVGEGSAVELVFPATIAA
SPTGSARIDARAAGVVRSVQKTLGDYVRQGETIARIESAEAAALASQLSAARARVNELSAAYERERRLFEANVTARQDLE
AVRANLQVAQSELSRAQAAVAAAGVSGDGRSLAVTSPLAGRVTAAPIVLGSFVDAGEELYQIVNPNAMQIEVALPSAEAS
RIQPGDEATLELAENREIGARVRSVTPSLDPESRSATAVLSLTRSIPGLQPGAFLQARIRPSGEVDTGRMSVPESAVQSL
DGQEVVFVRTRRGFQTRPVVTGARSAGRVVIESGLEANWRIATENAFLLKAELEKEEAEHGH

Sequences:

>Translated_382_residues
MNRKRLAVLIGLIILVFAAAVMLWPDNGAEPHSEEETEESDLPEGLVLLGEEQIRSSEIMVETVGEGSAVELVFPATIAA
SPTGSARIDARAAGVVRSVQKTLGDYVRQGETIARIESAEAAALASQLSAARARVNELSAAYERERRLFEANVTARQDLE
AVRANLQVAQSELSRAQAAVAAAGVSGDGRSLAVTSPLAGRVTAAPIVLGSFVDAGEELYQIVNPNAMQIEVALPSAEAS
RIQPGDEATLELAENREIGARVRSVTPSLDPESRSATAVLSLTRSIPGLQPGAFLQARIRPSGEVDTGRMSVPESAVQSL
DGQEVVFVRTRRGFQTRPVVTGARSAGRVVIESGLEANWRIATENAFLLKAELEKEEAEHGH
>Mature_382_residues
MNRKRLAVLIGLIILVFAAAVMLWPDNGAEPHSEEETEESDLPEGLVLLGEEQIRSSEIMVETVGEGSAVELVFPATIAA
SPTGSARIDARAAGVVRSVQKTLGDYVRQGETIARIESAEAAALASQLSAARARVNELSAAYERERRLFEANVTARQDLE
AVRANLQVAQSELSRAQAAVAAAGVSGDGRSLAVTSPLAGRVTAAPIVLGSFVDAGEELYQIVNPNAMQIEVALPSAEAS
RIQPGDEATLELAENREIGARVRSVTPSLDPESRSATAVLSLTRSIPGLQPGAFLQARIRPSGEVDTGRMSVPESAVQSL
DGQEVVFVRTRRGFQTRPVVTGARSAGRVVIESGLEANWRIATENAFLLKAELEKEEAEHGH

Specific function: Component of the NCC cation efflux system that confers resistance to nickel, cobalt and cadmium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005695
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40542; Mature: 40542

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKRLAVLIGLIILVFAAAVMLWPDNGAEPHSEEETEESDLPEGLVLLGEEQIRSSEIM
CCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHCCCCCEEEECHHHHCCCCEE
VETVGEGSAVELVFPATIAASPTGSARIDARAAGVVRSVQKTLGDYVRQGETIARIESAE
EEECCCCCEEEEEECCEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AAALASQLSAARARVNELSAAYERERRLFEANVTARQDLEAVRANLQVAQSELSRAQAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAGVSGDGRSLAVTSPLAGRVTAAPIVLGSFVDAGEELYQIVNPNAMQIEVALPSAEAS
HHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
RIQPGDEATLELAENREIGARVRSVTPSLDPESRSATAVLSLTRSIPGLQPGAFLQARIR
CCCCCCHHHHEECCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEC
PSGEVDTGRMSVPESAVQSLDGQEVVFVRTRRGFQTRPVVTGARSAGRVVIESGLEANWR
CCCCCCCCCCCCCHHHHHCCCCCEEEEEEECCCCCCCCEEECCCCCCEEEEECCCCCCEE
IATENAFLLKAELEKEEAEHGH
EECCCEEEEEEHHHHHHHCCCC
>Mature Secondary Structure
MNRKRLAVLIGLIILVFAAAVMLWPDNGAEPHSEEETEESDLPEGLVLLGEEQIRSSEIM
CCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHCCCCCEEEECHHHHCCCCEE
VETVGEGSAVELVFPATIAASPTGSARIDARAAGVVRSVQKTLGDYVRQGETIARIESAE
EEECCCCCEEEEEECCEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AAALASQLSAARARVNELSAAYERERRLFEANVTARQDLEAVRANLQVAQSELSRAQAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAGVSGDGRSLAVTSPLAGRVTAAPIVLGSFVDAGEELYQIVNPNAMQIEVALPSAEAS
HHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
RIQPGDEATLELAENREIGARVRSVTPSLDPESRSATAVLSLTRSIPGLQPGAFLQARIR
CCCCCCHHHHEECCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEC
PSGEVDTGRMSVPESAVQSLDGQEVVFVRTRRGFQTRPVVTGARSAGRVVIESGLEANWR
CCCCCCCCCCCCCHHHHHCCCCCEEEEEEECCCCCCCCEEECCCCCCEEEEECCCCCCEE
IATENAFLLKAELEKEEAEHGH
EECCCEEEEEEHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961470 [H]