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The map label for this gene is nusA

Identifier: 85373514

GI number: 85373514

Start: 709344

End: 711017

Strand: Direct

Name: nusA

Synonym: ELI_03435

Alternate gene names: 85373514

Gene position: 709344-711017 (Clockwise)

Preceding gene: 85373512

Following gene: 85373515

Centisome position: 23.24

GC content: 63.62

Gene sequence:

>1674_bases
ATGGCCAGTGCAATTTCCGCCAACAAGGCCGAGCTGCTCGCGATTGCGAATGCGGTCGCTTCGGAAAAGATGATCGACAA
GGCGATCGTCATCGAAGCGATGGAAGAAGCGATCCAGAAAAGCGCGCGCAACCGCTACGGCGCAGAGAACGACATTCGCG
CAAAGCTCGATCCGCAGACCGGCGATCTGCGCCTGTGGCGTGTCGTCGAAGTGGTCGAGGAAGTCGAAGACTATTTCAAG
CAGGTCGATCTGAAATCCGCGCAAAAGCTCGAAGAAGGCGCTGCGATCGGTGACTTCATCGTCGACCCGCTGCCGCCGGT
CGATCTCGGCCGTATCGACGCGCAGAGCGCGAAGCAGGTGATCTTCCAGAAGGTCCGCGACGCCGAGCGTGAGCGCCAGT
TCGAAGAATTCAAGGACCGCGCCGGCGAAGTCATCACCGGCGTCATCAAGTCGGTCGAGTTCGGCCACGTGATCGTCAAT
CTCGGCCGTGCCGAGGGCGTGATCCGCCGCGACCAGCAGATCCCGCGTGAAGCCGCGCGTGTCGGCGAGCGTGTCCGCGC
GATCATCACCAAGGTCGAGCGCAACAATCGCGGGCCGCAGATCTTCCTCAGCCGCGCGCATCCCGATTTCATGCGCAAGC
TGTTCGCGCAGGAAGTGCCCGAAATTTACGACGGCATCATCGAGATCAAGGCCGCCGCCCGCGACCCGGGGAGCCGCGCC
AAGATCGGCGTCATCAGCCACGATTCCAGCATTGACCCGGTCGGCGCCTGCGTCGGCATGAAGGGTAGCCGCGTCCAGGC
TGTCGTTCAGGAATTGCAGGGCGAGAAGATCGACATCATACCGTGGTCGGAAGATCAGGCCACGTTCATCGTCAACGCGT
TGCAGCCGGCGACGGTCAGCCGCGTGGTCCTCGACGAGGACGAGAGCCGGATCGAAGTCGTCGTGCCCGACGACCAGCTC
AGCCTCGCCATCGGTCGCCGCGGCCAGAATGTCCGGCTCGCCAGCCAGCTGACCGGCAACCAGATCGACATCATGACCGA
GGAAGAGGCGAGCGAGAAGCAGAGCAAGGAATTCGCCGAACGCTCCAAGATGTTCGAGGAAGAGCTCGACGTCGACGAAA
CGCTCTCGCAGTTGCTTGTCGCCGAAGGTTTCGCCGAGCTGGAAGAGGTCGCCTACGTGGCGATGGAAGAACTCGCCGCG
ATCGAAGGCTTCGACGAGGAGCTCGCCGAAGAACTCCAGAGCCGCGCGCAGGAAGCGCTCGAGCGTCAGGCCGAGGCCTA
TCGCAAGGAACGCCGCGAACTGGGCGTCGAAGACGATCTTGCCGAACTGCCCCACCTGTCGGAGCAGATGCTCGTGACGC
TGGGCAAAAGCGGTATCAAGACGCTCGACGACCTGGCCGACCTCGCCACCGACGAGCTGATCCAGAAGAAGCGCGAAGCC
CCGCGTCGCCGGAACACGGCCGAAGGTCCGCGCATGCGCAGCGATCGTCCGCAGCGCGAGCAGGACAAGGGCGGCGTGCT
CGGCGAATACGGTCTGACCGAAGAGCAAGGCAACGAGATCATCATGGCTGCCCGCGCGCACTGGTTCGAGGACGAGGAGA
AAGCCCCCGCCGCTTCCGCTCCCGCCGCCGAGCCTGACACTCAGGAGGCCGCCGATGCGGACTCCACCCAATGA

Upstream 100 bases:

>100_bases
AGCTGGTCCTCACCGACAAGCTGATCGCTGCGACACAACCCCTCGACGCTGACGGTGCTGAGGAAGTATTAGAAGACAAA
GAAGAGAAGGCTGACGACTG

Downstream 100 bases:

>100_bases
GCGCGTAAGCTCCGACATCTCTGACGGCACCAAGGCACGGACACCCAAGTCCGAGCCCGAACGCCGCTGCATCCTCTCCG
GAGAGAATGGCGCGCGCCAC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MASAISANKAELLAIANAVASEKMIDKAIVIEAMEEAIQKSARNRYGAENDIRAKLDPQTGDLRLWRVVEVVEEVEDYFK
QVDLKSAQKLEEGAAIGDFIVDPLPPVDLGRIDAQSAKQVIFQKVRDAERERQFEEFKDRAGEVITGVIKSVEFGHVIVN
LGRAEGVIRRDQQIPREAARVGERVRAIITKVERNNRGPQIFLSRAHPDFMRKLFAQEVPEIYDGIIEIKAAARDPGSRA
KIGVISHDSSIDPVGACVGMKGSRVQAVVQELQGEKIDIIPWSEDQATFIVNALQPATVSRVVLDEDESRIEVVVPDDQL
SLAIGRRGQNVRLASQLTGNQIDIMTEEEASEKQSKEFAERSKMFEEELDVDETLSQLLVAEGFAELEEVAYVAMEELAA
IEGFDEELAEELQSRAQEALERQAEAYRKERRELGVEDDLAELPHLSEQMLVTLGKSGIKTLDDLADLATDELIQKKREA
PRRRNTAEGPRMRSDRPQREQDKGGVLGEYGLTEEQGNEIIMAARAHWFEDEEKAPAASAPAAEPDTQEAADADSTQ

Sequences:

>Translated_557_residues
MASAISANKAELLAIANAVASEKMIDKAIVIEAMEEAIQKSARNRYGAENDIRAKLDPQTGDLRLWRVVEVVEEVEDYFK
QVDLKSAQKLEEGAAIGDFIVDPLPPVDLGRIDAQSAKQVIFQKVRDAERERQFEEFKDRAGEVITGVIKSVEFGHVIVN
LGRAEGVIRRDQQIPREAARVGERVRAIITKVERNNRGPQIFLSRAHPDFMRKLFAQEVPEIYDGIIEIKAAARDPGSRA
KIGVISHDSSIDPVGACVGMKGSRVQAVVQELQGEKIDIIPWSEDQATFIVNALQPATVSRVVLDEDESRIEVVVPDDQL
SLAIGRRGQNVRLASQLTGNQIDIMTEEEASEKQSKEFAERSKMFEEELDVDETLSQLLVAEGFAELEEVAYVAMEELAA
IEGFDEELAEELQSRAQEALERQAEAYRKERRELGVEDDLAELPHLSEQMLVTLGKSGIKTLDDLADLATDELIQKKREA
PRRRNTAEGPRMRSDRPQREQDKGGVLGEYGLTEEQGNEIIMAARAHWFEDEEKAPAASAPAAEPDTQEAADADSTQ
>Mature_556_residues
ASAISANKAELLAIANAVASEKMIDKAIVIEAMEEAIQKSARNRYGAENDIRAKLDPQTGDLRLWRVVEVVEEVEDYFKQ
VDLKSAQKLEEGAAIGDFIVDPLPPVDLGRIDAQSAKQVIFQKVRDAERERQFEEFKDRAGEVITGVIKSVEFGHVIVNL
GRAEGVIRRDQQIPREAARVGERVRAIITKVERNNRGPQIFLSRAHPDFMRKLFAQEVPEIYDGIIEIKAAARDPGSRAK
IGVISHDSSIDPVGACVGMKGSRVQAVVQELQGEKIDIIPWSEDQATFIVNALQPATVSRVVLDEDESRIEVVVPDDQLS
LAIGRRGQNVRLASQLTGNQIDIMTEEEASEKQSKEFAERSKMFEEELDVDETLSQLLVAEGFAELEEVAYVAMEELAAI
EGFDEELAEELQSRAQEALERQAEAYRKERRELGVEDDLAELPHLSEQMLVTLGKSGIKTLDDLADLATDELIQKKREAP
RRRNTAEGPRMRSDRPQREQDKGGVLGEYGLTEEQGNEIIMAARAHWFEDEEKAPAASAPAAEPDTQEAADADSTQ

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=524, Percent_Identity=41.2213740458015, Blast_Score=379, Evalue=1e-106,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 61899; Mature: 61768

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASAISANKAELLAIANAVASEKMIDKAIVIEAMEEAIQKSARNRYGAENDIRAKLDPQT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCC
GDLRLWRVVEVVEEVEDYFKQVDLKSAQKLEEGAAIGDFIVDPLPPVDLGRIDAQSAKQV
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHHHHHH
IFQKVRDAERERQFEEFKDRAGEVITGVIKSVEFGHVIVNLGRAEGVIRRDQQIPREAAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHCCHHHHHH
VGERVRAIITKVERNNRGPQIFLSRAHPDFMRKLFAQEVPEIYDGIIEIKAAARDPGSRA
HHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KIGVISHDSSIDPVGACVGMKGSRVQAVVQELQGEKIDIIPWSEDQATFIVNALQPATVS
EEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCHHHH
RVVLDEDESRIEVVVPDDQLSLAIGRRGQNVRLASQLTGNQIDIMTEEEASEKQSKEFAE
HHEECCCCCEEEEEECCCCEEHHCCCCCCCEEEHHHCCCCEEEEEECHHHHHHHHHHHHH
RSKMFEEELDVDETLSQLLVAEGFAELEEVAYVAMEELAAIEGFDEELAEELQSRAQEAL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ERQAEAYRKERRELGVEDDLAELPHLSEQMLVTLGKSGIKTLDDLADLATDELIQKKREA
HHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PRRRNTAEGPRMRSDRPQREQDKGGVLGEYGLTEEQGNEIIMAARAHWFEDEEKAPAASA
HHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHCCCEEEEEEHHHCCCCHHCCCCCCC
PAAEPDTQEAADADSTQ
CCCCCCCHHHCCCCCCC
>Mature Secondary Structure 
ASAISANKAELLAIANAVASEKMIDKAIVIEAMEEAIQKSARNRYGAENDIRAKLDPQT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCC
GDLRLWRVVEVVEEVEDYFKQVDLKSAQKLEEGAAIGDFIVDPLPPVDLGRIDAQSAKQV
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHHHHHH
IFQKVRDAERERQFEEFKDRAGEVITGVIKSVEFGHVIVNLGRAEGVIRRDQQIPREAAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHCCHHHHHH
VGERVRAIITKVERNNRGPQIFLSRAHPDFMRKLFAQEVPEIYDGIIEIKAAARDPGSRA
HHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KIGVISHDSSIDPVGACVGMKGSRVQAVVQELQGEKIDIIPWSEDQATFIVNALQPATVS
EEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCHHHH
RVVLDEDESRIEVVVPDDQLSLAIGRRGQNVRLASQLTGNQIDIMTEEEASEKQSKEFAE
HHEECCCCCEEEEEECCCCEEHHCCCCCCCEEEHHHCCCCEEEEEECHHHHHHHHHHHHH
RSKMFEEELDVDETLSQLLVAEGFAELEEVAYVAMEELAAIEGFDEELAEELQSRAQEAL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ERQAEAYRKERRELGVEDDLAELPHLSEQMLVTLGKSGIKTLDDLADLATDELIQKKREA
HHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PRRRNTAEGPRMRSDRPQREQDKGGVLGEYGLTEEQGNEIIMAARAHWFEDEEKAPAASA
HHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHCCCEEEEEEHHHCCCCHHCCCCCCC
PAAEPDTQEAADADSTQ
CCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]