Click here to switch to the map view.

The map label for this gene is ppk

Identifier: 85373477

GI number: 85373477

Start: 685795

End: 687942

Strand: Direct

Name: ppk

Synonym: ELI_03250

Alternate gene names: 85373477

Gene position: 685795-687942 (Clockwise)

Preceding gene: 85373476

Following gene: 85373478

Centisome position: 22.47

GC content: 62.34

Gene sequence:

>2148_bases
ATGGGCAGCCACCCGATTACGCAGGACAATGATCTCGACCCGCCTACGGAGCCGGGAGAGCGCTATTTCAACCGCGAATT
GAGCTGGCTCGCGTTCAACGACCGCGTGCTCGCCGAGGCCTGTAACGACAGCTATCCCTTGCTCGAGCGGCTGCGCTTCC
TGTCGATCTCGGGCAGCAATCTCGATGAGTTCGTGATGATCCGCGTGGCCGGCCTCGTTGGGCAGGTGCAGCGCGGGATC
GACGAGGTTTCTGACGACGGCCGCTCCCCGCGCGAGCAGTTGAACGCGGTGGTCGCCAGGCTGGAAGAACTGTCGGAGCG
ACAGCAGGATATCTGGCGGAACCTGCGGGTCCAGCTGGCCGATGCGGGCGTGCACGTCGCGGACGAAGAGCGGGTGAATG
CTGAGGCCTATGGGTGGCTCAAGCAGCACTTTTTCGAAAGCATCCTGCCGCTGCTGACCCCGCAGGCGCTCGATCCGGCG
CACCCCTTTCCCTTCATCTCGAACGAGGGGCTGGGCCTGCTCTTCACGCTGCGGCGCGGTGGCGAGGAACTGGTCGAGAT
GGTGCTGATCCCTTCGGCCCTGCCGCGCTTTATCCGCGTGCCGGGCGAAGATGCGCTCTATATCAGCATAGAGAGCCTCA
TCACGCGCTTTGCCAAGGAGCTATTCCCGGGTTTCGAGATCGTCGGCGACGGCACATTTCGCGTGTTGCGCGACAGCGAC
ATCGAAATCCAGGAAGAGGCCGAAGACCTTGTCCGTACCTTCCGCAGCGCGATCCAGCGCCGCCGCCGGGGGCAGGTCAT
CCAGCTCGAGATCGAAGAGGAATTCGATCCTACCGCCGAGGCGCTGCTGCGCGAAAAGCTCGACACGCCGGGCGCGACCT
TCGTGAAGACAGACGGCATGCTCGGCATCGCGGGGCTCGCCGATATCGTCGACGAAGACCGGCCCGACCTCAAGTTCGAC
AGCTATTCGCCGCGCTATCCCGAGCGTGTGCTGGAGCATGATGGCGACATCTTCGCGGCGATCCGCGAAAAGGATATCGT
CATTCACCACCCCTACGAAAGCTTCAACGTGGTGGTCGATTTCATCCGCCGCGCTGCGATCGATCCCGACGTGGTGGCGA
TCAAGCAGGCGCTCTACCGCGCGGGCACCCAATCCGAAGTCGTCGATGCGCTGGTCGAAGCGGCGGAGAACGGCAAGTCG
GTGACGGCGGTCGTCGAGTTGAAGGCGCGCTTCGACGAGGAGCAGAACCTCTACTGGGCCAACAAGCTCGAGCGGGCAGG
CGTTCAGGTGATCTACGGCTTCGTCGACTGGAAAACCCATGCCAAGGTCGCGATGGTCGTGCGCCGCGAAGAGGAGGGCT
TCCGGACCTATTGCCACTTCGGTACCGGCAATTACCACCCGATCACGGCCAAAATCTACACCGATCTCAGTTACTTCACC
GCCGATCCCCGACTGGGTCGAGATGCGGCCAAGCTGTTCAACTTCGTCACCGGCTATGTGGAACCGCGCGAGCTGGAGAT
GCTCGCCGTCTCGCCGATCGACCTGCGCGAAACGATCTATGCCGCGATCGATAACGAGATGGTCAATGCACAGGCCGGTA
AGCCGGCGGCGATCTGGATGAAGATGAACCAGATCACCGATGTCGACATGATCGACAGGCTTTACGAAGCCAGCCAGGCC
GGGGTCGAAATCCAATTGGTGGTGCGCGGGATTTGCAATCTGCGCCCCGGCGTGCCGGGCATGTCGGACAATATCCGCGT
CAAATCCATCATCGGGCGCTTTTTGGAGCACAGCCGCATCTACGCCTTCGCCAATGGCGAGCCGATGCCGGGGACGAGCT
CTACGGTATACATTTCGTCCGCCGACCTGATGGTGCGCAACCTCGACCGGCGGGTCGAGCAATTGGTGCCGATCGACAAC
CAGACGGTGCACGACCAGGTGTTGCAGCAGGTGCTGCTCGCCAACCTGCTCGACAACGAGCAAAGCTGGGAATTGCAGCC
AGACGGGAGCTACTTCCGGGTGGAAGTCGGCGAGAAGTCGTTCAATTGCCACCGCTATTTCATGACCAACCCGTCGCTCT
CGGGGCGCGGGGGTGCATTGGAGGCCGGAGCCGTGCCCAAGCTTGCCTTGCGGAAGGGTGCCGTCTGA

Upstream 100 bases:

>100_bases
CAAGCGCGGGATAAGCGAAATCGCGCTCGCCAAGGACCTCGTCTAGGAAGTTCACGCTACGGCTAGCAATTCTAAGGGCG
CGACCGCTATAGGAAGAGCC

Downstream 100 bases:

>100_bases
ATGGCGATCGGGGGGCGCCGTAGGGATCGGGACGGGCTCTTCATGGGCAACGTCCCGCACCGCGCGATCATTGATGTCGG
CTCCAACACGGTGCGCCTGG

Product: polyphosphate kinase

Products: NA

Alternate protein names: ATP-polyphosphate phosphotransferase; Polyphosphoric acid kinase

Number of amino acids: Translated: 715; Mature: 714

Protein sequence:

>715_residues
MGSHPITQDNDLDPPTEPGERYFNRELSWLAFNDRVLAEACNDSYPLLERLRFLSISGSNLDEFVMIRVAGLVGQVQRGI
DEVSDDGRSPREQLNAVVARLEELSERQQDIWRNLRVQLADAGVHVADEERVNAEAYGWLKQHFFESILPLLTPQALDPA
HPFPFISNEGLGLLFTLRRGGEELVEMVLIPSALPRFIRVPGEDALYISIESLITRFAKELFPGFEIVGDGTFRVLRDSD
IEIQEEAEDLVRTFRSAIQRRRRGQVIQLEIEEEFDPTAEALLREKLDTPGATFVKTDGMLGIAGLADIVDEDRPDLKFD
SYSPRYPERVLEHDGDIFAAIREKDIVIHHPYESFNVVVDFIRRAAIDPDVVAIKQALYRAGTQSEVVDALVEAAENGKS
VTAVVELKARFDEEQNLYWANKLERAGVQVIYGFVDWKTHAKVAMVVRREEEGFRTYCHFGTGNYHPITAKIYTDLSYFT
ADPRLGRDAAKLFNFVTGYVEPRELEMLAVSPIDLRETIYAAIDNEMVNAQAGKPAAIWMKMNQITDVDMIDRLYEASQA
GVEIQLVVRGICNLRPGVPGMSDNIRVKSIIGRFLEHSRIYAFANGEPMPGTSSTVYISSADLMVRNLDRRVEQLVPIDN
QTVHDQVLQQVLLANLLDNEQSWELQPDGSYFRVEVGEKSFNCHRYFMTNPSLSGRGGALEAGAVPKLALRKGAV

Sequences:

>Translated_715_residues
MGSHPITQDNDLDPPTEPGERYFNRELSWLAFNDRVLAEACNDSYPLLERLRFLSISGSNLDEFVMIRVAGLVGQVQRGI
DEVSDDGRSPREQLNAVVARLEELSERQQDIWRNLRVQLADAGVHVADEERVNAEAYGWLKQHFFESILPLLTPQALDPA
HPFPFISNEGLGLLFTLRRGGEELVEMVLIPSALPRFIRVPGEDALYISIESLITRFAKELFPGFEIVGDGTFRVLRDSD
IEIQEEAEDLVRTFRSAIQRRRRGQVIQLEIEEEFDPTAEALLREKLDTPGATFVKTDGMLGIAGLADIVDEDRPDLKFD
SYSPRYPERVLEHDGDIFAAIREKDIVIHHPYESFNVVVDFIRRAAIDPDVVAIKQALYRAGTQSEVVDALVEAAENGKS
VTAVVELKARFDEEQNLYWANKLERAGVQVIYGFVDWKTHAKVAMVVRREEEGFRTYCHFGTGNYHPITAKIYTDLSYFT
ADPRLGRDAAKLFNFVTGYVEPRELEMLAVSPIDLRETIYAAIDNEMVNAQAGKPAAIWMKMNQITDVDMIDRLYEASQA
GVEIQLVVRGICNLRPGVPGMSDNIRVKSIIGRFLEHSRIYAFANGEPMPGTSSTVYISSADLMVRNLDRRVEQLVPIDN
QTVHDQVLQQVLLANLLDNEQSWELQPDGSYFRVEVGEKSFNCHRYFMTNPSLSGRGGALEAGAVPKLALRKGAV
>Mature_714_residues
GSHPITQDNDLDPPTEPGERYFNRELSWLAFNDRVLAEACNDSYPLLERLRFLSISGSNLDEFVMIRVAGLVGQVQRGID
EVSDDGRSPREQLNAVVARLEELSERQQDIWRNLRVQLADAGVHVADEERVNAEAYGWLKQHFFESILPLLTPQALDPAH
PFPFISNEGLGLLFTLRRGGEELVEMVLIPSALPRFIRVPGEDALYISIESLITRFAKELFPGFEIVGDGTFRVLRDSDI
EIQEEAEDLVRTFRSAIQRRRRGQVIQLEIEEEFDPTAEALLREKLDTPGATFVKTDGMLGIAGLADIVDEDRPDLKFDS
YSPRYPERVLEHDGDIFAAIREKDIVIHHPYESFNVVVDFIRRAAIDPDVVAIKQALYRAGTQSEVVDALVEAAENGKSV
TAVVELKARFDEEQNLYWANKLERAGVQVIYGFVDWKTHAKVAMVVRREEEGFRTYCHFGTGNYHPITAKIYTDLSYFTA
DPRLGRDAAKLFNFVTGYVEPRELEMLAVSPIDLRETIYAAIDNEMVNAQAGKPAAIWMKMNQITDVDMIDRLYEASQAG
VEIQLVVRGICNLRPGVPGMSDNIRVKSIIGRFLEHSRIYAFANGEPMPGTSSTVYISSADLMVRNLDRRVEQLVPIDNQ
TVHDQVLQQVLLANLLDNEQSWELQPDGSYFRVEVGEKSFNCHRYFMTNPSLSGRGGALEAGAVPKLALRKGAV

Specific function: Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP)

COG id: COG0855

COG function: function code P; Polyphosphate kinase

Gene ontology:

Cell location: Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polyphosphate kinase family

Homologues:

Organism=Escherichia coli, GI1788847, Length=682, Percent_Identity=33.4310850439883, Blast_Score=384, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPK_ERYLH (Q2NC49)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457539.1
- ProteinModelPortal:   Q2NC49
- STRING:   Q2NC49
- GeneID:   3869197
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_03250
- NMPDR:   fig|314225.3.peg.2432
- eggNOG:   COG0855
- HOGENOM:   HBG341268
- OMA:   NYHAGNA
- PhylomeDB:   Q2NC49
- ProtClustDB:   PRK05443
- BioCyc:   ELIT314225:ELI_03250-MONOMER
- HAMAP:   MF_00347
- InterPro:   IPR003414
- PIRSF:   PIRSF015589
- TIGRFAMs:   TIGR03705

Pfam domain/function: PF02503 PP_kinase

EC number: =2.7.4.1

Molecular weight: Translated: 80562; Mature: 80431

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: ACT_SITE 440-440 ACT_SITE 459-459

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSHPITQDNDLDPPTEPGERYFNRELSWLAFNDRVLAEACNDSYPLLERLRFLSISGSN
CCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCHHHHHHCCCCCHHHHHHHHHCCCCCC
LDEFVMIRVAGLVGQVQRGIDEVSDDGRSPREQLNAVVARLEELSERQQDIWRNLRVQLA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
DAGVHVADEERVNAEAYGWLKQHFFESILPLLTPQALDPAHPFPFISNEGLGLLFTLRRG
CCCCEECCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCC
GEELVEMVLIPSALPRFIRVPGEDALYISIESLITRFAKELFPGFEIVGDGTFRVLRDSD
CHHHHHHHHHHHHHHHHEECCCCCEEEEEHHHHHHHHHHHHCCCEEEECCCEEEEEECCC
IEIQEEAEDLVRTFRSAIQRRRRGQVIQLEIEEEFDPTAEALLREKLDTPGATFVKTDGM
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEECCCC
LGIAGLADIVDEDRPDLKFDSYSPRYPERVLEHDGDIFAAIREKDIVIHHPYESFNVVVD
EEHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCEEEECCCHHHHHHHH
FIRRAAIDPDVVAIKQALYRAGTQSEVVDALVEAAENGKSVTAVVELKARFDEEQNLYWA
HHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEEEHHHCCCCCCCHHH
NKLERAGVQVIYGFVDWKTHAKVAMVVRREEEGFRTYCHFGTGNYHPITAKIYTDLSYFT
HHHHHCCEEEEEEECCCCCCCEEEEEEEECCCCCEEEEEECCCCCCEEEEEEEECHHHEE
ADPRLGRDAAKLFNFVTGYVEPRELEMLAVSPIDLRETIYAAIDNEMVNAQAGKPAAIWM
CCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHCCCHHCCCCCCCEEEEE
KMNQITDVDMIDRLYEASQAGVEIQLVVRGICNLRPGVPGMSDNIRVKSIIGRFLEHSRI
EECCCCCHHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCEE
YAFANGEPMPGTSSTVYISSADLMVRNLDRRVEQLVPIDNQTVHDQVLQQVLLANLLDNE
EEEECCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
QSWELQPDGSYFRVEVGEKSFNCHRYFMTNPSLSGRGGALEAGAVPKLALRKGAV
CCCEECCCCCEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCC
>Mature Secondary Structure 
GSHPITQDNDLDPPTEPGERYFNRELSWLAFNDRVLAEACNDSYPLLERLRFLSISGSN
CCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCHHHHHHCCCCCHHHHHHHHHCCCCCC
LDEFVMIRVAGLVGQVQRGIDEVSDDGRSPREQLNAVVARLEELSERQQDIWRNLRVQLA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
DAGVHVADEERVNAEAYGWLKQHFFESILPLLTPQALDPAHPFPFISNEGLGLLFTLRRG
CCCCEECCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCC
GEELVEMVLIPSALPRFIRVPGEDALYISIESLITRFAKELFPGFEIVGDGTFRVLRDSD
CHHHHHHHHHHHHHHHHEECCCCCEEEEEHHHHHHHHHHHHCCCEEEECCCEEEEEECCC
IEIQEEAEDLVRTFRSAIQRRRRGQVIQLEIEEEFDPTAEALLREKLDTPGATFVKTDGM
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEECCCC
LGIAGLADIVDEDRPDLKFDSYSPRYPERVLEHDGDIFAAIREKDIVIHHPYESFNVVVD
EEHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCEEEECCCHHHHHHHH
FIRRAAIDPDVVAIKQALYRAGTQSEVVDALVEAAENGKSVTAVVELKARFDEEQNLYWA
HHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEEEHHHCCCCCCCHHH
NKLERAGVQVIYGFVDWKTHAKVAMVVRREEEGFRTYCHFGTGNYHPITAKIYTDLSYFT
HHHHHCCEEEEEEECCCCCCCEEEEEEEECCCCCEEEEEECCCCCCEEEEEEEECHHHEE
ADPRLGRDAAKLFNFVTGYVEPRELEMLAVSPIDLRETIYAAIDNEMVNAQAGKPAAIWM
CCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHCCCHHCCCCCCCEEEEE
KMNQITDVDMIDRLYEASQAGVEIQLVVRGICNLRPGVPGMSDNIRVKSIIGRFLEHSRI
EECCCCCHHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCEE
YAFANGEPMPGTSSTVYISSADLMVRNLDRRVEQLVPIDNQTVHDQVLQQVLLANLLDNE
EEEECCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
QSWELQPDGSYFRVEVGEKSFNCHRYFMTNPSLSGRGGALEAGAVPKLALRKGAV
CCCEECCCCCEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA