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The map label for this gene is pbpE [H]

Identifier: 85373287

GI number: 85373287

Start: 499822

End: 501180

Strand: Reverse

Name: pbpE [H]

Synonym: ELI_02300

Alternate gene names: 85373287

Gene position: 501180-499822 (Counterclockwise)

Preceding gene: 85373290

Following gene: 85373283

Centisome position: 16.42

GC content: 56.88

Gene sequence:

>1359_bases
ATGAAATTGGTAGGGCTGCTTGGCTGTTCGTTGCTGACCTGCATGTCCGCAAATGTCGCGGCCGAGCCGGCCGCCAGCCA
TTTCGAGAAATGGCTCACCGCCTTCAATTCAGGTGATAAAGCGACAATCCAGAAGTTCTATGAAGAACATCTGGGAGACG
CCGATGCAGTGACCGCTCGGAATTTGCGCGCTGAGACGTGTGGGTTCCACCTGGCGCGAATTGAAGACGAAAGCGACAAA
TCCTTTCGCGCCCTTCTGAGCCAGCGGTGCTTTCCTGCACTTTTCCGAGTGAGCTTCGGAGTAGCGGATGACGGGGAGAC
ACTCGAGGAATTCAAGCTCCAACCGTTCGCCATGTCCGAGGCGCGGACGCTAGCGGCCACTCGGGACATTGCGGACCGGT
TGGCGGGATTGGACAGCTTCACTGGATCGATGATCGTGGTACGCGACGGGAAGGAGCCGTGGACCTATACGGCAGGAACC
TTCAGCAAAGACGATGAACGACCAATTACTCTAGAGACACCGATGTTTCTGGCATCCGCCGGCAAGATGTTCACCGCGGT
GGGTATTCTACAGCTTGTCGAGAAAGGCCAAGTTGAACTGGACGCTTCGCTTTCCCGCTACCTGTCAGATTATCCGAACA
GTGAAATGGCGAAAGTTACAATCAGGCAGTTGCTGGGCCATCGCGGCGGGACTGGCGAGGATGGCATCCTTCAGCGCGAA
GACGGTGCCAACCGGGCGTGGGTCCGGACCATCGACGATCTGATCAAGCTAAACGGCAGCCGTGCGCCTGCCTTTCCACC
GGGCAGCAAGTTCGATTATTCAAATTATGGGTTCATTCTGCTCGGCGCGGTGATTGAGCGAGTGTCGGGGCAGTCCTACC
AGAATTTTATCCAGCAGAACGTATTCGACCCTGCCGGAATGACGCATGCGTCGTTTCCGGACTTGGAACATATCGCGGAT
ATTCCCGTCGGCTATACGACCTTCTTTGAAAGCGAGCCTGAGCCCATTGCGGCAACCGATGTGCTCCCATGGAGAGGAAC
GGCTGCCGGGGGCGGAGTTGCCAGCGCGATCGACCTGTTGCGATTTTTTAACGCCCTCAGGTCGGACATTCTCCTGTCCA
GCGACACGCTTGCGATAGCTACCGAAGCCAGTGAGACGGGCTGGTACGGCCTCGGGTTTGTCACCCAGGGCGGCAAGCAT
CCGCTTTGGGGGCATGGCGGATTCGGATATGGAATGAACGTCGCGGCACAGAGATTTCCGGAGGATGAAGTCACATTCAT
CTGCGTTGCTACGCGCGATATGGCCTGCGACCGGATGATCTACGCGTGGTCTGATCGTCTATTTGGTGCAGTAGAGTAG

Upstream 100 bases:

>100_bases
AGTTTCTCAAGCTGATGCCACCAATATTTTGTACGCAGGGAATTTGGGGCATGAACGCGCTTCTTTGGACGCTACGGAAA
AATTTCGGGAGAGAGGTAGG

Downstream 100 bases:

>100_bases
CGTTTGGCTTTTCCGCTTTGCGATTAGCACATTGCTATATCTTGAAACCGGACAGGCTACTCGCGGAGTTCTTCGCAACA
AATGCGGCCTGTCAGCAAAC

Product: Beta-lactamase class C family protein

Products: NA

Alternate protein names: PBP 4*; PBP 4A; Penicillin-binding protein E [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MKLVGLLGCSLLTCMSANVAAEPAASHFEKWLTAFNSGDKATIQKFYEEHLGDADAVTARNLRAETCGFHLARIEDESDK
SFRALLSQRCFPALFRVSFGVADDGETLEEFKLQPFAMSEARTLAATRDIADRLAGLDSFTGSMIVVRDGKEPWTYTAGT
FSKDDERPITLETPMFLASAGKMFTAVGILQLVEKGQVELDASLSRYLSDYPNSEMAKVTIRQLLGHRGGTGEDGILQRE
DGANRAWVRTIDDLIKLNGSRAPAFPPGSKFDYSNYGFILLGAVIERVSGQSYQNFIQQNVFDPAGMTHASFPDLEHIAD
IPVGYTTFFESEPEPIAATDVLPWRGTAAGGGVASAIDLLRFFNALRSDILLSSDTLAIATEASETGWYGLGFVTQGGKH
PLWGHGGFGYGMNVAAQRFPEDEVTFICVATRDMACDRMIYAWSDRLFGAVE

Sequences:

>Translated_452_residues
MKLVGLLGCSLLTCMSANVAAEPAASHFEKWLTAFNSGDKATIQKFYEEHLGDADAVTARNLRAETCGFHLARIEDESDK
SFRALLSQRCFPALFRVSFGVADDGETLEEFKLQPFAMSEARTLAATRDIADRLAGLDSFTGSMIVVRDGKEPWTYTAGT
FSKDDERPITLETPMFLASAGKMFTAVGILQLVEKGQVELDASLSRYLSDYPNSEMAKVTIRQLLGHRGGTGEDGILQRE
DGANRAWVRTIDDLIKLNGSRAPAFPPGSKFDYSNYGFILLGAVIERVSGQSYQNFIQQNVFDPAGMTHASFPDLEHIAD
IPVGYTTFFESEPEPIAATDVLPWRGTAAGGGVASAIDLLRFFNALRSDILLSSDTLAIATEASETGWYGLGFVTQGGKH
PLWGHGGFGYGMNVAAQRFPEDEVTFICVATRDMACDRMIYAWSDRLFGAVE
>Mature_452_residues
MKLVGLLGCSLLTCMSANVAAEPAASHFEKWLTAFNSGDKATIQKFYEEHLGDADAVTARNLRAETCGFHLARIEDESDK
SFRALLSQRCFPALFRVSFGVADDGETLEEFKLQPFAMSEARTLAATRDIADRLAGLDSFTGSMIVVRDGKEPWTYTAGT
FSKDDERPITLETPMFLASAGKMFTAVGILQLVEKGQVELDASLSRYLSDYPNSEMAKVTIRQLLGHRGGTGEDGILQRE
DGANRAWVRTIDDLIKLNGSRAPAFPPGSKFDYSNYGFILLGAVIERVSGQSYQNFIQQNVFDPAGMTHASFPDLEHIAD
IPVGYTTFFESEPEPIAATDVLPWRGTAAGGGVASAIDLLRFFNALRSDILLSSDTLAIATEASETGWYGLGFVTQGGKH
PLWGHGGFGYGMNVAAQRFPEDEVTFICVATRDMACDRMIYAWSDRLFGAVE

Specific function: Probably involved in peptidoglycan modification during cortex synthesis [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Escherichia coli, GI87082101, Length=187, Percent_Identity=26.2032085561497, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790593, Length=257, Percent_Identity=23.7354085603113, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI115533026, Length=289, Percent_Identity=28.0276816608997, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17550934, Length=289, Percent_Identity=29.0657439446367, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR021860 [H]

Pfam domain/function: PF00144 Beta-lactamase; PF11954 DUF3471 [H]

EC number: NA

Molecular weight: Translated: 49355; Mature: 49355

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVGLLGCSLLTCMSANVAAEPAASHFEKWLTAFNSGDKATIQKFYEEHLGDADAVTAR
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHH
NLRAETCGFHLARIEDESDKSFRALLSQRCFPALFRVSFGVADDGETLEEFKLQPFAMSE
CCCHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHH
ARTLAATRDIADRLAGLDSFTGSMIVVRDGKEPWTYTAGTFSKDDERPITLETPMFLASA
HHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEECCCCCCCCCCCEEEECCHHHHHC
GKMFTAVGILQLVEKGQVELDASLSRYLSDYPNSEMAKVTIRQLLGHRGGTGEDGILQRE
CHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCEECC
DGANRAWVRTIDDLIKLNGSRAPAFPPGSKFDYSNYGFILLGAVIERVSGQSYQNFIQQN
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHH
VFDPAGMTHASFPDLEHIADIPVGYTTFFESEPEPIAATDVLPWRGTAAGGGVASAIDLL
CCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCEEECCCCCCCCCCCCCHHHHHHHHH
RFFNALRSDILLSSDTLAIATEASETGWYGLGFVTQGGKHPLWGHGGFGYGMNVAAQRFP
HHHHHHHHHHEECCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCC
EDEVTFICVATRDMACDRMIYAWSDRLFGAVE
CCCEEEEEEECCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKLVGLLGCSLLTCMSANVAAEPAASHFEKWLTAFNSGDKATIQKFYEEHLGDADAVTAR
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHH
NLRAETCGFHLARIEDESDKSFRALLSQRCFPALFRVSFGVADDGETLEEFKLQPFAMSE
CCCHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHH
ARTLAATRDIADRLAGLDSFTGSMIVVRDGKEPWTYTAGTFSKDDERPITLETPMFLASA
HHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEECCCCCCCCCCCEEEECCHHHHHC
GKMFTAVGILQLVEKGQVELDASLSRYLSDYPNSEMAKVTIRQLLGHRGGTGEDGILQRE
CHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCEECC
DGANRAWVRTIDDLIKLNGSRAPAFPPGSKFDYSNYGFILLGAVIERVSGQSYQNFIQQN
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHH
VFDPAGMTHASFPDLEHIADIPVGYTTFFESEPEPIAATDVLPWRGTAAGGGVASAIDLL
CCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCEEECCCCCCCCCCCCCHHHHHHHHH
RFFNALRSDILLSSDTLAIATEASETGWYGLGFVTQGGKHPLWGHGGFGYGMNVAAQRFP
HHHHHHHHHHEECCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCC
EDEVTFICVATRDMACDRMIYAWSDRLFGAVE
CCCEEEEEEECCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8491712; 9384377 [H]