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The map label for this gene is fmt [H]

Identifier: 85373240

GI number: 85373240

Start: 458220

End: 459110

Strand: Reverse

Name: fmt [H]

Synonym: ELI_02065

Alternate gene names: 85373240

Gene position: 459110-458220 (Counterclockwise)

Preceding gene: 85373244

Following gene: 85373239

Centisome position: 15.04

GC content: 65.21

Gene sequence:

>891_bases
ATGGGAACTCCGGACTTCGCCGTGCCGACCCTGCGCGCGCTGCACGCGGCGGGGCATGAGGTGGTTGCGGCCTATACCCA
GCCGCCGCGTCCAGCGGGTCGGGGCAAGAAGCTGCAGCCCTCGCCCGTCCAGAAAGCGGCGGAAGAACTAGGGATCGAAG
TCCGCTCGCCCACATCGCTGAAAAAGGCCGACGAGCAGGAAGCCTTTGCTGCGATAAATGCGGACGTCGCAGTGGTCGCT
GCCTATGGTTTGATCCTGCCGCAGCCGATCCTTGACGCGCCTAAGCACGGTTGTCTCAATGTCCACGCCTCGATCCTGCC
GCGCTGGCGCGGGGCGGCGCCGATCCACCGCGCGATCATGGCGGGTGATGCGGTGACCGGGGTCACGATCATGCAGATGG
AAGCGGGGCTCGATACTGGGCCGATGCTGGCGACGATCCGCACACCTATCAACGACAAGACGACTGGCGAATTGACAGAG
GAACTGGCCGAACTCGGCGCAAACCTGATGGTCCAGACCTTGCGCGAACTCGACAAGCATGTGCCTATCGCGCAGGACAA
CGCGGAAGCGACATATGCGCCCAAGATCGACAAGGCCGAGGCGCGGATCGACTGGGCGAAACCGGCCGAAGAGGTGGTGC
GGCACATCCACGGGCTCGCCCCGTTTCCCGGAGCATGGTGCGAGATCGAGGGCGAGCGGGTGAAGCTCTTGCGCGCAGAG
ATGGCGGGGGACGAGGGCGCAGCGGGTGAGGTACTTGACGATCAGTTCACCATCGCCTGCGGTACCGATGCGATCCGTCC
GCTCCGCCTCCAGCGCGCCGGGAAGCCAGCCATGGACCTGGACGATTTCCTGCGCGGCAAGGCGGTGGCCAAGGGCACCG
TTCTATCCTGA

Upstream 100 bases:

>100_bases
CTTGTCGAAGCCCTGCTCTTTCTTTTTCCCTCGCTGAAAGTATGGACAGCCCTTCGACAGGCTCAGGGCGAACGGAATAG
GCAAGTTGCGCACAATCTTC

Downstream 100 bases:

>100_bases
TCCGTTCGTATCGAGCGGAGAACGCAGTTCGCAGTCGAGATGCCGTTGGCATAGGTGTCTCGACTATGCTCGACACGAAC
GGCTAGGGGGCGTGCCATGA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MGTPDFAVPTLRALHAAGHEVVAAYTQPPRPAGRGKKLQPSPVQKAAEELGIEVRSPTSLKKADEQEAFAAINADVAVVA
AYGLILPQPILDAPKHGCLNVHASILPRWRGAAPIHRAIMAGDAVTGVTIMQMEAGLDTGPMLATIRTPINDKTTGELTE
ELAELGANLMVQTLRELDKHVPIAQDNAEATYAPKIDKAEARIDWAKPAEEVVRHIHGLAPFPGAWCEIEGERVKLLRAE
MAGDEGAAGEVLDDQFTIACGTDAIRPLRLQRAGKPAMDLDDFLRGKAVAKGTVLS

Sequences:

>Translated_296_residues
MGTPDFAVPTLRALHAAGHEVVAAYTQPPRPAGRGKKLQPSPVQKAAEELGIEVRSPTSLKKADEQEAFAAINADVAVVA
AYGLILPQPILDAPKHGCLNVHASILPRWRGAAPIHRAIMAGDAVTGVTIMQMEAGLDTGPMLATIRTPINDKTTGELTE
ELAELGANLMVQTLRELDKHVPIAQDNAEATYAPKIDKAEARIDWAKPAEEVVRHIHGLAPFPGAWCEIEGERVKLLRAE
MAGDEGAAGEVLDDQFTIACGTDAIRPLRLQRAGKPAMDLDDFLRGKAVAKGTVLS
>Mature_295_residues
GTPDFAVPTLRALHAAGHEVVAAYTQPPRPAGRGKKLQPSPVQKAAEELGIEVRSPTSLKKADEQEAFAAINADVAVVAA
YGLILPQPILDAPKHGCLNVHASILPRWRGAAPIHRAIMAGDAVTGVTIMQMEAGLDTGPMLATIRTPINDKTTGELTEE
LAELGANLMVQTLRELDKHVPIAQDNAEATYAPKIDKAEARIDWAKPAEEVVRHIHGLAPFPGAWCEIEGERVKLLRAEM
AGDEGAAGEVLDDQFTIACGTDAIRPLRLQRAGKPAMDLDDFLRGKAVAKGTVLS

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI164663775, Length=238, Percent_Identity=33.6134453781513, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI238814322, Length=262, Percent_Identity=30.5343511450382, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI21614513, Length=250, Percent_Identity=28, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1789683, Length=294, Percent_Identity=48.9795918367347, Blast_Score=267, Evalue=7e-73,
Organism=Escherichia coli, GI1788589, Length=269, Percent_Identity=29.368029739777, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI133930964, Length=258, Percent_Identity=28.2945736434109, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319458, Length=331, Percent_Identity=26.8882175226586, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45550868, Length=302, Percent_Identity=30.4635761589404, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI28571984, Length=225, Percent_Identity=34.6666666666667, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24585660, Length=164, Percent_Identity=29.8780487804878, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 31535; Mature: 31403

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTPDFAVPTLRALHAAGHEVVAAYTQPPRPAGRGKKLQPSPVQKAAEELGIEVRSPTSL
CCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHH
KKADEQEAFAAINADVAVVAAYGLILPQPILDAPKHGCLNVHASILPRWRGAAPIHRAIM
HHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHCCCCCCCEEEHHHHCCCCCCCCHHHHHHH
AGDAVTGVTIMQMEAGLDTGPMLATIRTPINDKTTGELTEELAELGANLMVQTLRELDKH
HCCCHHCEEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
VPIAQDNAEATYAPKIDKAEARIDWAKPAEEVVRHIHGLAPFPGAWCEIEGERVKLLRAE
CCCCCCCCCCEECCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHH
MAGDEGAAGEVLDDQFTIACGTDAIRPLRLQRAGKPAMDLDDFLRGKAVAKGTVLS
HCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCCCHHHHHCCCHHCCCCCCC
>Mature Secondary Structure 
GTPDFAVPTLRALHAAGHEVVAAYTQPPRPAGRGKKLQPSPVQKAAEELGIEVRSPTSL
CCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHH
KKADEQEAFAAINADVAVVAAYGLILPQPILDAPKHGCLNVHASILPRWRGAAPIHRAIM
HHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHCCCCCCCEEEHHHHCCCCCCCCHHHHHHH
AGDAVTGVTIMQMEAGLDTGPMLATIRTPINDKTTGELTEELAELGANLMVQTLRELDKH
HCCCHHCEEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
VPIAQDNAEATYAPKIDKAEARIDWAKPAEEVVRHIHGLAPFPGAWCEIEGERVKLLRAE
CCCCCCCCCCEECCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHH
MAGDEGAAGEVLDDQFTIACGTDAIRPLRLQRAGKPAMDLDDFLRGKAVAKGTVLS
HCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCCCHHHHHCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA