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The map label for this gene is truA

Identifier: 85373239

GI number: 85373239

Start: 457380

End: 458123

Strand: Reverse

Name: truA

Synonym: ELI_02060

Alternate gene names: 85373239

Gene position: 458123-457380 (Counterclockwise)

Preceding gene: 85373240

Following gene: 85373238

Centisome position: 15.01

GC content: 62.37

Gene sequence:

>744_bases
ATGACCCGCTTCGCGCTAACCCTCGAATTCGACGGCACGCCGTTCATGGGCCTGCAGCGCCAGAAGCATGGGCCGAGCGT
GCAACAAGCCGTCGAAGAAGCGGCGCGGGCCACGCTCAATCAGGATATAACGCTCCACAGCGCCGGGCGCACCGATACCG
GTGTCCATGCCCTCGGCATGCGTAGCCATTTCGATGCCGAAACTGACCTGACGCCGTTCCGCCTGATGGGCGGGCTGAAT
GCGCACCTCCGGCCCCACCCCATAGCTGTAACGAATTGCGAGATAATGCCCGAAGATTGGCATGCGCGCTTTGCTTGCAT
CGGGCGCAGTTATGTCTATCGCATCATCAACCGCCGCGCGCCGCTGACGATCGACAGGAAACGCGCCTGGCAGGTGCCGC
AACAGCTCGATCACGAAGCCATGCAGCGCGCGGCGCAATTGCTGGTCGGTACGCATGATTTCACGACGTTCCGCTCGACC
CAGTGCCAAGCGAAGGACCCGGTCAAATCGCTCGACCGGCTCGAAGTCGAACGCGACGGGGACGAGATACGGGTTCATGC
CGAAGCACGCAGTTTCCTCCATCACCAGGTCCGCTCGATGGTCGGCTGTCTCAAGCTGGTCGGACAGGGCACGTGGCGCG
AGGAAGAGGTCGAGGAAGCTTTGCTTGCCCGCGACAGACAGCGTTTGGGGTTGAATGCCCCGCCCCATGGCCTCTACTTC
GTGGCCGCAGAATACCCCCGATAA

Upstream 100 bases:

>100_bases
CTGATCCGTTCGTATCGAGCGGAGAACGCAGTTCGCAGTCGAGATGCCGTTGGCATAGGTGTCTCGACTATGCTCGACAC
GAACGGCTAGGGGGCGTGCC

Downstream 100 bases:

>100_bases
GAAACACACCCGAGGAGACGACCCGATGAGCACCACCGGCAAACAGCTATTCACCACCCTGGAAGCGGACGGTACGCTCA
CGGTCGAGATCGAGGAAGTG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MTRFALTLEFDGTPFMGLQRQKHGPSVQQAVEEAARATLNQDITLHSAGRTDTGVHALGMRSHFDAETDLTPFRLMGGLN
AHLRPHPIAVTNCEIMPEDWHARFACIGRSYVYRIINRRAPLTIDRKRAWQVPQQLDHEAMQRAAQLLVGTHDFTTFRST
QCQAKDPVKSLDRLEVERDGDEIRVHAEARSFLHHQVRSMVGCLKLVGQGTWREEEVEEALLARDRQRLGLNAPPHGLYF
VAAEYPR

Sequences:

>Translated_247_residues
MTRFALTLEFDGTPFMGLQRQKHGPSVQQAVEEAARATLNQDITLHSAGRTDTGVHALGMRSHFDAETDLTPFRLMGGLN
AHLRPHPIAVTNCEIMPEDWHARFACIGRSYVYRIINRRAPLTIDRKRAWQVPQQLDHEAMQRAAQLLVGTHDFTTFRST
QCQAKDPVKSLDRLEVERDGDEIRVHAEARSFLHHQVRSMVGCLKLVGQGTWREEEVEEALLARDRQRLGLNAPPHGLYF
VAAEYPR
>Mature_246_residues
TRFALTLEFDGTPFMGLQRQKHGPSVQQAVEEAARATLNQDITLHSAGRTDTGVHALGMRSHFDAETDLTPFRLMGGLNA
HLRPHPIAVTNCEIMPEDWHARFACIGRSYVYRIINRRAPLTIDRKRAWQVPQQLDHEAMQRAAQLLVGTHDFTTFRSTQ
CQAKDPVKSLDRLEVERDGDEIRVHAEARSFLHHQVRSMVGCLKLVGQGTWREEEVEEALLARDRQRLGLNAPPHGLYFV
AAEYPR

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=274, Percent_Identity=33.5766423357664, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI1788657, Length=246, Percent_Identity=39.4308943089431, Blast_Score=173, Evalue=1e-44,
Organism=Drosophila melanogaster, GI116007752, Length=302, Percent_Identity=27.8145695364238, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_ERYLH (Q2NCT7)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457301.1
- ProteinModelPortal:   Q2NCT7
- SMR:   Q2NCT7
- STRING:   Q2NCT7
- GeneID:   3869593
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_02060
- NMPDR:   fig|314225.3.peg.2675
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   HSAIRRH
- PhylomeDB:   Q2NCT7
- ProtClustDB:   PRK00021
- BioCyc:   ELIT314225:ELI_02060-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28093; Mature: 27962

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRFALTLEFDGTPFMGLQRQKHGPSVQQAVEEAARATLNQDITLHSAGRTDTGVHALGM
CCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECC
RSHFDAETDLTPFRLMGGLNAHLRPHPIAVTNCEIMPEDWHARFACIGRSYVYRIINRRA
CCCCCCCCCCCHHHHHCCCCCCCCCCCEEEECCEECCCCHHHHHHHHHHHHHHHHHHCCC
PLTIDRKRAWQVPQQLDHEAMQRAAQLLVGTHDFTTFRSTQCQAKDPVKSLDRLEVERDG
CCEECHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCHHHHHHHHHHCCCC
DEIRVHAEARSFLHHQVRSMVGCLKLVGQGTWREEEVEEALLARDRQRLGLNAPPHGLYF
CEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEE
VAAEYPR
EEECCCC
>Mature Secondary Structure 
TRFALTLEFDGTPFMGLQRQKHGPSVQQAVEEAARATLNQDITLHSAGRTDTGVHALGM
CEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECC
RSHFDAETDLTPFRLMGGLNAHLRPHPIAVTNCEIMPEDWHARFACIGRSYVYRIINRRA
CCCCCCCCCCCHHHHHCCCCCCCCCCCEEEECCEECCCCHHHHHHHHHHHHHHHHHHCCC
PLTIDRKRAWQVPQQLDHEAMQRAAQLLVGTHDFTTFRSTQCQAKDPVKSLDRLEVERDG
CCEECHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCHHHHHHHHHHCCCC
DEIRVHAEARSFLHHQVRSMVGCLKLVGQGTWREEEVEEALLARDRQRLGLNAPPHGLYF
CEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEE
VAAEYPR
EEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA