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The map label for this gene is sseA [H]

Identifier: 85373208

GI number: 85373208

Start: 426712

End: 427560

Strand: Reverse

Name: sseA [H]

Synonym: ELI_01905

Alternate gene names: 85373208

Gene position: 427560-426712 (Counterclockwise)

Preceding gene: 85373209

Following gene: 85373207

Centisome position: 14.01

GC content: 65.49

Gene sequence:

>849_bases
ATGGACGCGCTCGTTTCGACAGATTGGCTGGCAGGGCAATTCGGCTCACCCGATCTCGTCGTGCTCGACGCCTCGCTGCA
CCTGCCGCAAGCCGGGCGCGACGCCGAAGCTGAGTTCGAAAAGGGTCACATCCCGGGCGCGCGATTTCTGCATCTCGCCG
CGCTTCATGATCCGGCCTCGTCGCTGCCCGGCAAAATTCCCGAACCCGACGCTCTTGCCGAGCGGCTTTCGGCGCTGGGC
GCCGAGCCGGAGAGCCGCATCGTCCTCTACGACGACAGCGCCCTGCGGACGGCGTGCCGGGCGTGGGCCCTGCTCCGTTT
CGCCGGTCTCGAAAAGATCGCCGTGCTCGATGGCGGTTTCGCCAAATGGCGAAGCGAGAATCGGGCCATCGAAACCGGGC
CGGGCGAGTGCGGCCACGGTGCTGCGCCCGACCTCCATGTCGAGCGTGCAAGACTGCGCGACAAAGCCCAGATGCTCGCC
AATTGCGCCGATAGCGCGGAGCAAGTGATCGATGCGCGCGATGCCGGCCGCTTTGCCGGTACGGTCGAGGACACGGTCCA
TAACCTCCCGGGCGGACATATTCCCGGAGCCTGCAACCTGCCTTTCACCGCGCTTTTCCGCGAGGACGGCACCTTCAAAC
CGGAAAATGAGCTTCGCACCGCTTTCGAGGATGCCGGCATCGACCTTTCACGCCCCATTGTGACCTCCTGCGGCAGCGGC
GTCACCGCATCGGTGTTGATCTTTGCACTGCACCTGCTGGGCATCGAAGACGCCGCGCTCTACGATGGCAGCTGGTCCGA
ATGGGGGGCCGATCCGGAAACGCCCAAGGCAACGGGAGTGGCAGGCTGA

Upstream 100 bases:

>100_bases
TCTGCAGCCGTTCCGGCTCGATACGCCGCCGCAGGGATTCAGCGATGTCGGCGCCCGCGCGCTGACTTGACCGACCGCGC
CGACGCGCTAGGCCACGGCG

Downstream 100 bases:

>100_bases
GCGACAAGAAAAAACTGCGCACGGGCACAAAGCTGGTCGAAGCCGGTCGCCGCGAGGAATGGACCGGGCCGGTCGTCAAT
CCGCCGGTCTGGCGCGCCAG

Product: thiosulfate sulfurtransferase, putative

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MDALVSTDWLAGQFGSPDLVVLDASLHLPQAGRDAEAEFEKGHIPGARFLHLAALHDPASSLPGKIPEPDALAERLSALG
AEPESRIVLYDDSALRTACRAWALLRFAGLEKIAVLDGGFAKWRSENRAIETGPGECGHGAAPDLHVERARLRDKAQMLA
NCADSAEQVIDARDAGRFAGTVEDTVHNLPGGHIPGACNLPFTALFREDGTFKPENELRTAFEDAGIDLSRPIVTSCGSG
VTASVLIFALHLLGIEDAALYDGSWSEWGADPETPKATGVAG

Sequences:

>Translated_282_residues
MDALVSTDWLAGQFGSPDLVVLDASLHLPQAGRDAEAEFEKGHIPGARFLHLAALHDPASSLPGKIPEPDALAERLSALG
AEPESRIVLYDDSALRTACRAWALLRFAGLEKIAVLDGGFAKWRSENRAIETGPGECGHGAAPDLHVERARLRDKAQMLA
NCADSAEQVIDARDAGRFAGTVEDTVHNLPGGHIPGACNLPFTALFREDGTFKPENELRTAFEDAGIDLSRPIVTSCGSG
VTASVLIFALHLLGIEDAALYDGSWSEWGADPETPKATGVAG
>Mature_282_residues
MDALVSTDWLAGQFGSPDLVVLDASLHLPQAGRDAEAEFEKGHIPGARFLHLAALHDPASSLPGKIPEPDALAERLSALG
AEPESRIVLYDDSALRTACRAWALLRFAGLEKIAVLDGGFAKWRSENRAIETGPGECGHGAAPDLHVERARLRDKAQMLA
NCADSAEQVIDARDAGRFAGTVEDTVHNLPGGHIPGACNLPFTALFREDGTFKPENELRTAFEDAGIDLSRPIVTSCGSG
VTASVLIFALHLLGIEDAALYDGSWSEWGADPETPKATGVAG

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=272, Percent_Identity=36.3970588235294, Blast_Score=173, Evalue=1e-43,
Organism=Homo sapiens, GI61835204, Length=272, Percent_Identity=36.3970588235294, Blast_Score=173, Evalue=1e-43,
Organism=Homo sapiens, GI194473681, Length=272, Percent_Identity=36.3970588235294, Blast_Score=173, Evalue=1e-43,
Organism=Homo sapiens, GI17402865, Length=287, Percent_Identity=37.9790940766551, Blast_Score=171, Evalue=9e-43,
Organism=Escherichia coli, GI87082121, Length=273, Percent_Identity=39.5604395604396, Blast_Score=197, Evalue=9e-52,
Organism=Escherichia coli, GI87081967, Length=254, Percent_Identity=25.1968503937008, Blast_Score=78, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71997283, Length=308, Percent_Identity=24.3506493506494, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17561888, Length=312, Percent_Identity=23.7179487179487, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI115534702, Length=266, Percent_Identity=22.9323308270677, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324825, Length=269, Percent_Identity=28.996282527881, Blast_Score=112, Evalue=8e-26,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 29852; Mature: 29852

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALVSTDWLAGQFGSPDLVVLDASLHLPQAGRDAEAEFEKGHIPGARFLHLAALHDPAS
CCCCCCCCHHCCCCCCCCEEEEECCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHCCCHH
SLPGKIPEPDALAERLSALGAEPESRIVLYDDSALRTACRAWALLRFAGLEKIAVLDGGF
HCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEECCCH
AKWRSENRAIETGPGECGHGAAPDLHVERARLRDKAQMLANCADSAEQVIDARDAGRFAG
HHHHCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TVEDTVHNLPGGHIPGACNLPFTALFREDGTFKPENELRTAFEDAGIDLSRPIVTSCGSG
HHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHCCCC
VTASVLIFALHLLGIEDAALYDGSWSEWGADPETPKATGVAG
HHHHHHHHHHHHHCCCHHHEECCCCHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDALVSTDWLAGQFGSPDLVVLDASLHLPQAGRDAEAEFEKGHIPGARFLHLAALHDPAS
CCCCCCCCHHCCCCCCCCEEEEECCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHCCCHH
SLPGKIPEPDALAERLSALGAEPESRIVLYDDSALRTACRAWALLRFAGLEKIAVLDGGF
HCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEECCCH
AKWRSENRAIETGPGECGHGAAPDLHVERARLRDKAQMLANCADSAEQVIDARDAGRFAG
HHHHCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TVEDTVHNLPGGHIPGACNLPFTALFREDGTFKPENELRTAFEDAGIDLSRPIVTSCGSG
HHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHCCCC
VTASVLIFALHLLGIEDAALYDGSWSEWGADPETPKATGVAG
HHHHHHHHHHHHHCCCHHHEECCCCHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]