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The map label for this gene is nemA [H]

Identifier: 85372912

GI number: 85372912

Start: 110209

End: 111318

Strand: Reverse

Name: nemA [H]

Synonym: ELI_00425

Alternate gene names: 85372912

Gene position: 111318-110209 (Counterclockwise)

Preceding gene: 85372914

Following gene: 85372911

Centisome position: 3.65

GC content: 62.97

Gene sequence:

>1110_bases
ATGCACGACAGCCTGTTCCAGCCGCTGACCATGGGCGCCCTTGAGACGAAGAACCGGATTTTCATGGCTCCGCTGACCCG
CGGCCGCGCCGCCGAGCCGATGTTCGTGCCGAACGAAATGATGGCGACCTATTACCGCCAGCGCGCGGGGGCCGGCATGA
TTTTGACAGAGGCGACCGGTATCAGCCGCGAAGGCCTCGGCTGGCCTTCCGCACCCGGTATCTGGAGCGACGAACAGGCA
GAGGCGTGGAAGCCAACCACCGATGCCGTCCACGAAGAAGGGGGGCTGATCGTCTTGCAGCTTTGGCACATGGGACGGAT
TGTCCACCCTTACTTCCTCGACGGGGAACCACCCGTCTCCGCCAGCGCCACCAAGGCTCCCGGCGAAGCGCATACGCCGG
TCGGCAAACAGGAATATGCCACAGCGCGCGCAATGACGCCGGAGGATATCCAACGTACTATCGGCGACTATCGCAACGCA
GCCGAGAAAGCGAAGCAGGCCGGGTTCGACGGCGTGCAACTGCATGGTGCCAATGGCTATCTGATCGACCAGTTCCTGCG
CGACGGAACCAATCTGCGGACCGACGAATATGGCGGTCCGCCCGAAAACCGCACCCGCTTCATGCGCGAAGTATTGGAAG
CACTGATCGATGTCTGGGGTGCCGACAGGGTCGGCATCCGCCTGTCGCCCAACGGCGAAACGCAGGGCTGCGACGACAGC
GATCCGGCGACAGCATTCGGCGCGGCGGCCAAGGTTTGCGAAGACCTCGGCCTCGCCTTCGTCGAGTTGCGCGAGCCTGG
GCCGGAAGGAACCTTCGGCCAGACCGATGTGCCGAAACAGAGCCCGCTGATCCGGTCGCTCTATTCGGGCGCGCTGATCC
TCAATTCCGATTACACGGCGGAAGAGGCCGAAGCCGATGTCTCCAGCGGGAAATGCGACGCGGTCAGCTTCGGTCGACCC
TATATTTCCAACCCCGATCTCGAGAGGCGCATCGCGGCGGGCGCGCATACGAACCCGAACAAGGATGTGCCCAAAAGCTG
GTACTTCCCGATCCCCGAGGGTTACATCGATTATCCCACTTTGGAAGAGGAGCAGGCGCAGGTCGCCTAG

Upstream 100 bases:

>100_bases
CGCTTTGTCCCCTGACACTTTTTCGACGGTTGCACCCTCCATGCCGGTGCATTAGCCGCACTTCACGAAATCGAAAAAGA
ATTTGCGGAGAGATGCGAGA

Downstream 100 bases:

>100_bases
CGCCCCTGCCCCACCCTATGGCGCACCGATCCTGGCTCCTCGTCCCGGCCGACAACGACGCCAAGCTGGCTAAAGTACCG
GCTGTGGGCGCCGATGTCGT

Product: putative oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MHDSLFQPLTMGALETKNRIFMAPLTRGRAAEPMFVPNEMMATYYRQRAGAGMILTEATGISREGLGWPSAPGIWSDEQA
EAWKPTTDAVHEEGGLIVLQLWHMGRIVHPYFLDGEPPVSASATKAPGEAHTPVGKQEYATARAMTPEDIQRTIGDYRNA
AEKAKQAGFDGVQLHGANGYLIDQFLRDGTNLRTDEYGGPPENRTRFMREVLEALIDVWGADRVGIRLSPNGETQGCDDS
DPATAFGAAAKVCEDLGLAFVELREPGPEGTFGQTDVPKQSPLIRSLYSGALILNSDYTAEEAEADVSSGKCDAVSFGRP
YISNPDLERRIAAGAHTNPNKDVPKSWYFPIPEGYIDYPTLEEEQAQVA

Sequences:

>Translated_369_residues
MHDSLFQPLTMGALETKNRIFMAPLTRGRAAEPMFVPNEMMATYYRQRAGAGMILTEATGISREGLGWPSAPGIWSDEQA
EAWKPTTDAVHEEGGLIVLQLWHMGRIVHPYFLDGEPPVSASATKAPGEAHTPVGKQEYATARAMTPEDIQRTIGDYRNA
AEKAKQAGFDGVQLHGANGYLIDQFLRDGTNLRTDEYGGPPENRTRFMREVLEALIDVWGADRVGIRLSPNGETQGCDDS
DPATAFGAAAKVCEDLGLAFVELREPGPEGTFGQTDVPKQSPLIRSLYSGALILNSDYTAEEAEADVSSGKCDAVSFGRP
YISNPDLERRIAAGAHTNPNKDVPKSWYFPIPEGYIDYPTLEEEQAQVA
>Mature_369_residues
MHDSLFQPLTMGALETKNRIFMAPLTRGRAAEPMFVPNEMMATYYRQRAGAGMILTEATGISREGLGWPSAPGIWSDEQA
EAWKPTTDAVHEEGGLIVLQLWHMGRIVHPYFLDGEPPVSASATKAPGEAHTPVGKQEYATARAMTPEDIQRTIGDYRNA
AEKAKQAGFDGVQLHGANGYLIDQFLRDGTNLRTDEYGGPPENRTRFMREVLEALIDVWGADRVGIRLSPNGETQGCDDS
DPATAFGAAAKVCEDLGLAFVELREPGPEGTFGQTDVPKQSPLIRSLYSGALILNSDYTAEEAEADVSSGKCDAVSFGRP
YISNPDLERRIAAGAHTNPNKDVPKSWYFPIPEGYIDYPTLEEEQAQVA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=368, Percent_Identity=43.75, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1789463, Length=345, Percent_Identity=30.4347826086957, Blast_Score=124, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17565138, Length=307, Percent_Identity=29.9674267100977, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17559804, Length=359, Percent_Identity=27.2980501392758, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=352, Percent_Identity=26.9886363636364, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI72001454, Length=368, Percent_Identity=27.7173913043478, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17559802, Length=267, Percent_Identity=31.4606741573034, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=266, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17566914, Length=355, Percent_Identity=25.6338028169014, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6325086, Length=378, Percent_Identity=37.3015873015873, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6321973, Length=379, Percent_Identity=36.1477572559367, Blast_Score=191, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40264; Mature: 40264

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDSLFQPLTMGALETKNRIFMAPLTRGRAAEPMFVPNEMMATYYRQRAGAGMILTEATG
CCCCHHCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCC
ISREGLGWPSAPGIWSDEQAEAWKPTTDAVHEEGGLIVLQLWHMGRIVHPYFLDGEPPVS
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEEEHHCCCCCCCEEECCCCCCC
ASATKAPGEAHTPVGKQEYATARAMTPEDIQRTIGDYRNAAEKAKQAGFDGVQLHGANGY
CCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCH
LIDQFLRDGTNLRTDEYGGPPENRTRFMREVLEALIDVWGADRVGIRLSPNGETQGCDDS
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCC
DPATAFGAAAKVCEDLGLAFVELREPGPEGTFGQTDVPKQSPLIRSLYSGALILNSDYTA
CCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCEEEECCCCCH
EEAEADVSSGKCDAVSFGRPYISNPDLERRIAAGAHTNPNKDVPKSWYFPIPEGYIDYPT
HHHHCCCCCCCCCEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LEEEQAQVA
CCHHHHCCC
>Mature Secondary Structure
MHDSLFQPLTMGALETKNRIFMAPLTRGRAAEPMFVPNEMMATYYRQRAGAGMILTEATG
CCCCHHCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCC
ISREGLGWPSAPGIWSDEQAEAWKPTTDAVHEEGGLIVLQLWHMGRIVHPYFLDGEPPVS
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEEEHHCCCCCCCEEECCCCCCC
ASATKAPGEAHTPVGKQEYATARAMTPEDIQRTIGDYRNAAEKAKQAGFDGVQLHGANGY
CCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCH
LIDQFLRDGTNLRTDEYGGPPENRTRFMREVLEALIDVWGADRVGIRLSPNGETQGCDDS
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCC
DPATAFGAAAKVCEDLGLAFVELREPGPEGTFGQTDVPKQSPLIRSLYSGALILNSDYTA
CCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCEEEECCCCCH
EEAEADVSSGKCDAVSFGRPYISNPDLERRIAAGAHTNPNKDVPKSWYFPIPEGYIDYPT
HHHHCCCCCCCCCEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LEEEQAQVA
CCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]