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The map label for this gene is mcl2 [H]

Identifier: 85372911

GI number: 85372911

Start: 109331

End: 110191

Strand: Reverse

Name: mcl2 [H]

Synonym: ELI_00420

Alternate gene names: 85372911

Gene position: 110191-109331 (Counterclockwise)

Preceding gene: 85372912

Following gene: 85372908

Centisome position: 3.61

GC content: 65.27

Gene sequence:

>861_bases
ATGGCGCACCGATCCTGGCTCCTCGTCCCGGCCGACAACGACGCCAAGCTGGCTAAAGTACCGGCTGTGGGCGCCGATGT
CGTAGTTCTCGATCTCGCAGCGGTTGCCGAGCGCAACAAGCTCGAAGCGCGCGAGCATGCGGTCACATTCATGACCAAGA
TGCAGCGTCAGCTGACGGCGAAGAAGCCGTTTGCGCAATGGGTGCGGATCAATCCGATCGGCAGCCCGCACTGGAAAGAC
GACCTGATCGCCGCGATGGCGCAGAAGCCTGCGGGGATCGTGCTGCCCCGGGCGATGGGACCGCAGCAGGTGCAAATGCT
GGCCGCCGAGATTTACGAACTCGAACAGGGCGGCCCCGCCGCGCACAATTCGACCCGTATCCTGCCGCAGGTCGGCGATG
CACCCGAAGCGGCGCTGATGATTTCCGATTTCGCGAAGGATCCCCACCCGCGCATCATCGGCCTGTCGTGGGACGCGGGC
GCACTCGCCGCTGCTTCCCATATGAACACCGTTGACAACGACGCGATCCGGGCAATCCGCGCGCAATTGCTGCTCACGGC
CAAGGCGCGGGGCCTGCTGGCGATCGAAAGCCCGAGCGGGTTGGTCAAGCGGCAGGAAGCGCTCGAGGCGGTCGTCAATC
GCGCGCGGATAAATGGGTTCGACGGGATGATGGCGCGGCATCCGGCCCAGATCGCCGCCATCGACAAGGCCTTTGCCCCG
ACCGAGGAAGAACTGGTCGCGGCTCGAGCAATTCTTTCGGCATTCGAGCTGCATCCGGAAGCGCAGGTGCTGACAGTAGA
CGGCCGCGCCGTGGACCGAATCGGTCTGGAACGCGCGCGGCGTCTGCTCGACGCGGGCTAG

Upstream 100 bases:

>100_bases
TGCCCAAAAGCTGGTACTTCCCGATCCCCGAGGGTTACATCGATTATCCCACTTTGGAAGAGGAGCAGGCGCAGGTCGCC
TAGCGCCCCTGCCCCACCCT

Downstream 100 bases:

>100_bases
CCTTCGTCCTCGGCGGTTTCGACCGGCTCGCCAGCCGGCTCGGGCGTAGCTTGCGGCGCGTTATCGCTGGTTGGGTCCGG
AGATCCGGCGTCGCCGCCGC

Product: citrate lyase beta chain

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MAHRSWLLVPADNDAKLAKVPAVGADVVVLDLAAVAERNKLEAREHAVTFMTKMQRQLTAKKPFAQWVRINPIGSPHWKD
DLIAAMAQKPAGIVLPRAMGPQQVQMLAAEIYELEQGGPAAHNSTRILPQVGDAPEAALMISDFAKDPHPRIIGLSWDAG
ALAAASHMNTVDNDAIRAIRAQLLLTAKARGLLAIESPSGLVKRQEALEAVVNRARINGFDGMMARHPAQIAAIDKAFAP
TEEELVAARAILSAFELHPEAQVLTVDGRAVDRIGLERARRLLDAG

Sequences:

>Translated_286_residues
MAHRSWLLVPADNDAKLAKVPAVGADVVVLDLAAVAERNKLEAREHAVTFMTKMQRQLTAKKPFAQWVRINPIGSPHWKD
DLIAAMAQKPAGIVLPRAMGPQQVQMLAAEIYELEQGGPAAHNSTRILPQVGDAPEAALMISDFAKDPHPRIIGLSWDAG
ALAAASHMNTVDNDAIRAIRAQLLLTAKARGLLAIESPSGLVKRQEALEAVVNRARINGFDGMMARHPAQIAAIDKAFAP
TEEELVAARAILSAFELHPEAQVLTVDGRAVDRIGLERARRLLDAG
>Mature_285_residues
AHRSWLLVPADNDAKLAKVPAVGADVVVLDLAAVAERNKLEAREHAVTFMTKMQRQLTAKKPFAQWVRINPIGSPHWKDD
LIAAMAQKPAGIVLPRAMGPQQVQMLAAEIYELEQGGPAAHNSTRILPQVGDAPEAALMISDFAKDPHPRIIGLSWDAGA
LAAASHMNTVDNDAIRAIRAQLLLTAKARGLLAIESPSGLVKRQEALEAVVNRARINGFDGMMARHPAQIAAIDKAFAPT
EEELVAARAILSAFELHPEAQVLTVDGRAVDRIGLERARRLLDAG

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=303, Percent_Identity=25.4125412541254, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 30820; Mature: 30689

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHRSWLLVPADNDAKLAKVPAVGADVVVLDLAAVAERNKLEAREHAVTFMTKMQRQLTA
CCCCCEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKPFAQWVRINPIGSPHWKDDLIAAMAQKPAGIVLPRAMGPQQVQMLAAEIYELEQGGPA
CCCHHHEEEECCCCCCCCHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCC
AHNSTRILPQVGDAPEAALMISDFAKDPHPRIIGLSWDAGALAAASHMNTVDNDAIRAIR
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHCCCCCHHHHHHHH
AQLLLTAKARGLLAIESPSGLVKRQEALEAVVNRARINGFDGMMARHPAQIAAIDKAFAP
HHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHCCC
TEEELVAARAILSAFELHPEAQVLTVDGRAVDRIGLERARRLLDAG
CHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AHRSWLLVPADNDAKLAKVPAVGADVVVLDLAAVAERNKLEAREHAVTFMTKMQRQLTA
CCCCEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKPFAQWVRINPIGSPHWKDDLIAAMAQKPAGIVLPRAMGPQQVQMLAAEIYELEQGGPA
CCCHHHEEEECCCCCCCCHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCC
AHNSTRILPQVGDAPEAALMISDFAKDPHPRIIGLSWDAGALAAASHMNTVDNDAIRAIR
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHCCCCCHHHHHHHH
AQLLLTAKARGLLAIESPSGLVKRQEALEAVVNRARINGFDGMMARHPAQIAAIDKAFAP
HHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHCCC
TEEELVAARAILSAFELHPEAQVLTVDGRAVDRIGLERARRLLDAG
CHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA