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The map label for this gene is iorA [H]

Identifier: 85372903

GI number: 85372903

Start: 102179

End: 102730

Strand: Direct

Name: iorA [H]

Synonym: ELI_00380

Alternate gene names: 85372903

Gene position: 102179-102730 (Clockwise)

Preceding gene: 85372899

Following gene: 85372905

Centisome position: 3.35

GC content: 66.67

Gene sequence:

>552_bases
ATGCCCCGCCTGACGGTCAACGATCGCCCTGTCGAATACGATATGGACCCGGAGATTCCGCTGCTCTGGGCCTTGCGCGA
TGCCTCCAATCTTACCGGCACCAAATATGGCTGCGGCACCGGCGATTGCGGCGCCTGCATGGTCATTATCGATGGTGCAG
CGCTGCGCAGCTGCCTGATCACGCTGGAAGAGGCCGAGGGACGGTTCGTCACCACGATCGAAGGGCTGAGCCGCGATCGC
TCGCATCCGGTGCAACAGGCGATGGTGGCAGAGCAGGCAATCCAGTGCGGCTTCTGCACCCCCGGCATCGTGATGGCGGC
GGCGGCGCTGATCCAGCGCAATGCCGCACCGTCGAAGGAAGAGATCGAGCAGGCGATTCCGAACCTGTGTCGCTGCGGCG
TCTATCCACGGCTGGTCCGCGCGATCCAGCGCGCCGGGCGCGTCGAGCGCAGGCAGGAACGCATCAGTGCCGCGCCCGCA
CCGGGCATCGATCCCGCCGATGCAGCCGAGGCGGTGCCGGCGATGACACCGCCCGAGCCGGACGAAGAGTGA

Upstream 100 bases:

>100_bases
GTCTCGCCTAGCTGCGGGGTGGGTCAGGGGGAAGCGAAAATGCCGGATAGCGCGCTCGACGGCTTTGCGCTGGCTATCGA
ATTTGGCTTAGACAGCGCAC

Downstream 100 bases:

>100_bases
GCCCCCGCGACGGCGGGGGCTCACGCATCGGTCAGATTTTCACCCGCACGCCGAAGCGCGCACTGAAGGGCTCGGCCGGC
TGGATATTGTTGTCGCCATG

Product: isoquinoline 1-oxidoreductase (alpha subunit)oxidoreductase protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MPRLTVNDRPVEYDMDPEIPLLWALRDASNLTGTKYGCGTGDCGACMVIIDGAALRSCLITLEEAEGRFVTTIEGLSRDR
SHPVQQAMVAEQAIQCGFCTPGIVMAAAALIQRNAAPSKEEIEQAIPNLCRCGVYPRLVRAIQRAGRVERRQERISAAPA
PGIDPADAAEAVPAMTPPEPDEE

Sequences:

>Translated_183_residues
MPRLTVNDRPVEYDMDPEIPLLWALRDASNLTGTKYGCGTGDCGACMVIIDGAALRSCLITLEEAEGRFVTTIEGLSRDR
SHPVQQAMVAEQAIQCGFCTPGIVMAAAALIQRNAAPSKEEIEQAIPNLCRCGVYPRLVRAIQRAGRVERRQERISAAPA
PGIDPADAAEAVPAMTPPEPDEE
>Mature_182_residues
PRLTVNDRPVEYDMDPEIPLLWALRDASNLTGTKYGCGTGDCGACMVIIDGAALRSCLITLEEAEGRFVTTIEGLSRDRS
HPVQQAMVAEQAIQCGFCTPGIVMAAAALIQRNAAPSKEEIEQAIPNLCRCGVYPRLVRAIQRAGRVERRQERISAAPAP
GIDPADAAEAVPAMTPPEPDEE

Specific function: Specific towards N-containing N-heterocyclic substrates, including isoquinoline, isoquinolin-5-ol, phthalazine and quinazoline [H]

COG id: COG2080

COG function: function code C; Aerobic-type carbon monoxide dehydrogenase, small subunit CoxS/CutS homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 2Fe-2S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI91823271, Length=156, Percent_Identity=39.7435897435897, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI71773480, Length=152, Percent_Identity=40.7894736842105, Blast_Score=67, Evalue=7e-12,
Organism=Escherichia coli, GI1786480, Length=159, Percent_Identity=36.4779874213836, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1789232, Length=143, Percent_Identity=40.5594405594406, Blast_Score=99, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17539860, Length=152, Percent_Identity=40.1315789473684, Blast_Score=117, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32566215, Length=152, Percent_Identity=40.1315789473684, Blast_Score=114, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17540638, Length=149, Percent_Identity=40.9395973154362, Blast_Score=110, Evalue=5e-25,
Organism=Drosophila melanogaster, GI17737937, Length=146, Percent_Identity=41.0958904109589, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24647193, Length=158, Percent_Identity=33.5443037974684, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24647199, Length=138, Percent_Identity=34.0579710144928, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24647201, Length=151, Percent_Identity=33.112582781457, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24647195, Length=187, Percent_Identity=32.0855614973262, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24647197, Length=187, Percent_Identity=32.0855614973262, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002888
- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR001041 [H]

Pfam domain/function: PF00111 Fer2; PF01799 Fer2_2 [H]

EC number: =1.3.99.16 [H]

Molecular weight: Translated: 19687; Mature: 19556

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLTVNDRPVEYDMDPEIPLLWALRDASNLTGTKYGCGTGDCGACMVIIDGAALRSCLI
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHH
TLEEAEGRFVTTIEGLSRDRSHPVQQAMVAEQAIQCGFCTPGIVMAAAALIQRNAAPSKE
HHHCCCCCEEEHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHH
EIEQAIPNLCRCGVYPRLVRAIQRAGRVERRQERISAAPAPGIDPADAAEAVPAMTPPEP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCC
DEE
CCC
>Mature Secondary Structure 
PRLTVNDRPVEYDMDPEIPLLWALRDASNLTGTKYGCGTGDCGACMVIIDGAALRSCLI
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHH
TLEEAEGRFVTTIEGLSRDRSHPVQQAMVAEQAIQCGFCTPGIVMAAAALIQRNAAPSKE
HHHCCCCCEEEHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHH
EIEQAIPNLCRCGVYPRLVRAIQRAGRVERRQERISAAPAPGIDPADAAEAVPAMTPPEP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCC
DEE
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7782304; 8157655 [H]