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The map label for this gene is yngJ [H]

Identifier: 85372887

GI number: 85372887

Start: 81900

End: 82964

Strand: Direct

Name: yngJ [H]

Synonym: ELI_00300

Alternate gene names: 85372887

Gene position: 81900-82964 (Clockwise)

Preceding gene: 85372886

Following gene: 85372888

Centisome position: 2.68

GC content: 63.38

Gene sequence:

>1065_bases
ATGGCAGTGCTGAACGAAGAACAGACCATGCTGCGCGACATGGCGCAGGAATGGACGAAGAACGAAAGCCCGGTCGCCGC
GTGGCGCAAGGTCCGCAACGAGGGCAAGAATTTCGATCCCGAAGCCTGGGACGCGATGGCCGAAATGGGCTGGACCGGCG
TCATCATCCCCGAAAACTTCGGCGGCAGCGACTTCGGCTGGATGAGCCTCGGGCTGGTGGTCGAGGAAACCGGCAAGACG
CTGACGGCGAGCCCGCTGGTGGCAAGCTCGCTCGCGGCAAGCGCAATCATCCTCGGCGGCTCGCAGGCGCAGAAGGAAAA
ATACCTGCCCCGGCTCGCCAGTGGTGAACTGGTCGGCACGCTCGCCTTCGACGATGCCGCGCGGCACACCGGCGCAACGT
CGAGCGCGACGGTTTCGGACGGCAAGCTGAGCGGCACCAAGCCTTTCGTGCACGAGGCCGAAAGCGCCGGGCTGTTTGTG
GTCCTCGCCAGCGACGGTGTGTATTTCGTAGAGAAGGGGGATGGTGTTGCGCTGTCCGATCGCAACCTCACCGACCAGCG
CGACCATGCCGAAGTGACATTCGACGGCGCGCCTGCGGAAAAGCTGGCCGATGGCGGCGACGATCTCGCCGAGCAGATCC
TCGACCGTGCGCGCATTCTTACCGCTGCCGAAATGCTCGGCATGGCGCAGCAGGTCTTCGATGAAACGCTCGCCTATCTG
AAGCAGCGAACGCAGTTCAACCAGGTGCTATCGAGTTTTCAGGCGCTGCAGCACCGCATGGCCGACCTGTTCACCGATCT
CGAGCTGATGCGTACCGCTGTCGAGGCCGGGTTGCAGGCGCTCGATACCGGGTTCAAGGTGCCCGAGGCGGCCTGCCTCG
CCAAGAGCCGCGCCAACGATGTGCTGCACACGATGAGCCGGCAGGGTATCCAACTTCATGGCGGGATCGGGATGACTGAC
GAATACGATGTTGGGTTTTACCTGAAGCGCGCCCGTGTTCTCGAAGCGAGCTGGGGCAATTCGGGCTTCCTCAAGGATCG
CCACGCCAAGCTCGCCGGCTATTGA

Upstream 100 bases:

>100_bases
ATGGCGGCTCGACCGAAATCCAGAACAACATCATCGCCAAGCGTATCCTTGGCATGCTGGACCACCAGTAAGCGCGGGCG
CAGGGACAGGACGAAACATC

Downstream 100 bases:

>100_bases
GGCCGGCCCGCGACATGCTCGGCACCGCTAATCCGTTCGGGCTGAGCCTGTCGAAGCCTTGTCTTTCACTTTGAGCTCTA
CGCCAGAAGAAAGGACAGCC

Product: acyl-CoA dehydrogenase family protein

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MAVLNEEQTMLRDMAQEWTKNESPVAAWRKVRNEGKNFDPEAWDAMAEMGWTGVIIPENFGGSDFGWMSLGLVVEETGKT
LTASPLVASSLAASAIILGGSQAQKEKYLPRLASGELVGTLAFDDAARHTGATSSATVSDGKLSGTKPFVHEAESAGLFV
VLASDGVYFVEKGDGVALSDRNLTDQRDHAEVTFDGAPAEKLADGGDDLAEQILDRARILTAAEMLGMAQQVFDETLAYL
KQRTQFNQVLSSFQALQHRMADLFTDLELMRTAVEAGLQALDTGFKVPEAACLAKSRANDVLHTMSRQGIQLHGGIGMTD
EYDVGFYLKRARVLEASWGNSGFLKDRHAKLAGY

Sequences:

>Translated_354_residues
MAVLNEEQTMLRDMAQEWTKNESPVAAWRKVRNEGKNFDPEAWDAMAEMGWTGVIIPENFGGSDFGWMSLGLVVEETGKT
LTASPLVASSLAASAIILGGSQAQKEKYLPRLASGELVGTLAFDDAARHTGATSSATVSDGKLSGTKPFVHEAESAGLFV
VLASDGVYFVEKGDGVALSDRNLTDQRDHAEVTFDGAPAEKLADGGDDLAEQILDRARILTAAEMLGMAQQVFDETLAYL
KQRTQFNQVLSSFQALQHRMADLFTDLELMRTAVEAGLQALDTGFKVPEAACLAKSRANDVLHTMSRQGIQLHGGIGMTD
EYDVGFYLKRARVLEASWGNSGFLKDRHAKLAGY
>Mature_353_residues
AVLNEEQTMLRDMAQEWTKNESPVAAWRKVRNEGKNFDPEAWDAMAEMGWTGVIIPENFGGSDFGWMSLGLVVEETGKTL
TASPLVASSLAASAIILGGSQAQKEKYLPRLASGELVGTLAFDDAARHTGATSSATVSDGKLSGTKPFVHEAESAGLFVV
LASDGVYFVEKGDGVALSDRNLTDQRDHAEVTFDGAPAEKLADGGDDLAEQILDRARILTAAEMLGMAQQVFDETLAYLK
QRTQFNQVLSSFQALQHRMADLFTDLELMRTAVEAGLQALDTGFKVPEAACLAKSRANDVLHTMSRQGIQLHGGIGMTDE
YDVGFYLKRARVLEASWGNSGFLKDRHAKLAGY

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI226958412, Length=373, Percent_Identity=30.5630026809651, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI226958414, Length=335, Percent_Identity=29.8507462686567, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI4501859, Length=359, Percent_Identity=26.4623955431755, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI4501857, Length=333, Percent_Identity=26.4264264264264, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI21361497, Length=356, Percent_Identity=26.4044943820225, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI4557231, Length=368, Percent_Identity=25.8152173913043, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI187960098, Length=368, Percent_Identity=25.8152173913043, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI76496475, Length=391, Percent_Identity=26.8542199488491, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI4557235, Length=390, Percent_Identity=26.4102564102564, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI4557233, Length=365, Percent_Identity=25.7534246575342, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI7656849, Length=369, Percent_Identity=26.8292682926829, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI7669494, Length=363, Percent_Identity=26.9972451790634, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI4503943, Length=380, Percent_Identity=27.1052631578947, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1786223, Length=363, Percent_Identity=24.2424242424242, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17506239, Length=375, Percent_Identity=29.6, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17508101, Length=331, Percent_Identity=29.3051359516616, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17538396, Length=370, Percent_Identity=27.2972972972973, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI71990804, Length=282, Percent_Identity=29.7872340425532, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17569725, Length=367, Percent_Identity=25.6130790190736, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI32563615, Length=230, Percent_Identity=32.6086956521739, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17534899, Length=321, Percent_Identity=25.8566978193146, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71985184, Length=349, Percent_Identity=26.647564469914, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17534353, Length=346, Percent_Identity=24.5664739884393, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17533517, Length=313, Percent_Identity=25.8785942492013, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17570075, Length=319, Percent_Identity=24.7648902821317, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71985192, Length=289, Percent_Identity=26.9896193771626, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24666513, Length=367, Percent_Identity=27.7929155313352, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24660351, Length=370, Percent_Identity=27.8378378378378, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24646207, Length=366, Percent_Identity=23.7704918032787, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI19920834, Length=357, Percent_Identity=25.7703081232493, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 38301; Mature: 38170

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVLNEEQTMLRDMAQEWTKNESPVAAWRKVRNEGKNFDPEAWDAMAEMGWTGVIIPENF
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCC
GGSDFGWMSLGLVVEETGKTLTASPLVASSLAASAIILGGSQAQKEKYLPRLASGELVGT
CCCCCCHHHHCEEEECCCCEEECCHHHHHHHHHHHEEECCCHHHHHHHCCCCCCCCEEEE
LAFDDAARHTGATSSATVSDGKLSGTKPFVHEAESAGLFVVLASDGVYFVEKGDGVALSD
EEECHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEEECCCEEEEECCCCEEECC
RNLTDQRDHAEVTFDGAPAEKLADGGDDLAEQILDRARILTAAEMLGMAQQVFDETLAYL
CCCCCCCCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQRTQFNQVLSSFQALQHRMADLFTDLELMRTAVEAGLQALDTGFKVPEAACLAKSRAND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VLHTMSRQGIQLHGGIGMTDEYDVGFYLKRARVLEASWGNSGFLKDRHAKLAGY
HHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHEECCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
AVLNEEQTMLRDMAQEWTKNESPVAAWRKVRNEGKNFDPEAWDAMAEMGWTGVIIPENF
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCC
GGSDFGWMSLGLVVEETGKTLTASPLVASSLAASAIILGGSQAQKEKYLPRLASGELVGT
CCCCCCHHHHCEEEECCCCEEECCHHHHHHHHHHHEEECCCHHHHHHHCCCCCCCCEEEE
LAFDDAARHTGATSSATVSDGKLSGTKPFVHEAESAGLFVVLASDGVYFVEKGDGVALSD
EEECHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEEECCCEEEEECCCCEEECC
RNLTDQRDHAEVTFDGAPAEKLADGGDDLAEQILDRARILTAAEMLGMAQQVFDETLAYL
CCCCCCCCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQRTQFNQVLSSFQALQHRMADLFTDLELMRTAVEAGLQALDTGFKVPEAACLAKSRAND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VLHTMSRQGIQLHGGIGMTDEYDVGFYLKRARVLEASWGNSGFLKDRHAKLAGY
HHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHEECCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]