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The map label for this gene is agaV [H]

Identifier: 85060400

GI number: 85060400

Start: 4150120

End: 4150569

Strand: Reverse

Name: agaV [H]

Synonym: SG2422

Alternate gene names: 85060400

Gene position: 4150569-4150120 (Counterclockwise)

Preceding gene: 85060402

Following gene: 85060399

Centisome position: 99.51

GC content: 56.44

Gene sequence:

>450_bases
TTGATAACCTGCTGGTACATGGCCAGGGGGTGTGACCTGGGCCAATTCCCTGAGCGCGAATTTATTGCTGGTGGCCAACG
ATCAGGCCGCCGCCGATCCGATACAGCAAAGTTTAATGGATATGGTGTGTCGCCGGGCGTGGAAACCCGCTATTTCACTC
TGCAAAAAACCATCGACGTTATTCATCGTGCCGCACCACGTCAGCGAATTTTCATCGTCTGCAAAACCCCGCAGGACGTG
CTGACGCTGGTGCGCGGCGATGTGCCAATCCAAGCGGTCAACGTCGGTAATATGCATTTCGCCGAGGGCAAACGGCAGAT
TCATAAGACTGTCTCGGTGGATGACGACGATATTGCGGCATTTCGCGAACTGGCGCGTCTGGGCGTGCGATGCGAAATTC
GCCGGGTACCGGACGAAAGCGGTGAGCCAGTCGACCGGCTCTTGGACTGA

Upstream 100 bases:

>100_bases
GGGCGTGACACTTTATCCCTATCCAGCCGGCCAAGGCGCCGGTCAACGCAAACAGCAGGAGGAAGACGCATGACCGCGCC
AAATATCTTACTGACCCGGA

Downstream 100 bases:

>100_bases
CGGAAGGAGAAGTCTATGTTTACCGAGGCGCTGTTGATTGGTCTGCTGGCGGGCCTGGCCGGCGTGGATATGTTTAACGG
GCTGACGCACTTTCACCGAC

Product: PTS system mannose/fructose/sorbose-specific IIB component

Products: NA

Alternate protein names: EIIB-Aga'; PTS system N-acetylgalactosamine-specific EIIB component 2 [H]

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MITCWYMARGCDLGQFPEREFIAGGQRSGRRRSDTAKFNGYGVSPGVETRYFTLQKTIDVIHRAAPRQRIFIVCKTPQDV
LTLVRGDVPIQAVNVGNMHFAEGKRQIHKTVSVDDDDIAAFRELARLGVRCEIRRVPDESGEPVDRLLD

Sequences:

>Translated_149_residues
MITCWYMARGCDLGQFPEREFIAGGQRSGRRRSDTAKFNGYGVSPGVETRYFTLQKTIDVIHRAAPRQRIFIVCKTPQDV
LTLVRGDVPIQAVNVGNMHFAEGKRQIHKTVSVDDDDIAAFRELARLGVRCEIRRVPDESGEPVDRLLD
>Mature_149_residues
MITCWYMARGCDLGQFPEREFIAGGQRSGRRRSDTAKFNGYGVSPGVETRYFTLQKTIDVIHRAAPRQRIFIVCKTPQDV
LTLVRGDVPIQAVNVGNMHFAEGKRQIHKTVSVDDDDIAAFRELARLGVRCEIRRVPDESGEPVDRLLD

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI87082217, Length=106, Percent_Identity=56.6037735849057, Blast_Score=126, Evalue=6e-31,
Organism=Escherichia coli, GI1789527, Length=110, Percent_Identity=46.3636363636364, Blast_Score=104, Evalue=2e-24,
Organism=Escherichia coli, GI1788120, Length=108, Percent_Identity=37.962962962963, Blast_Score=70, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 16848; Mature: 16848

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITCWYMARGCDLGQFPEREFIAGGQRSGRRRSDTAKFNGYGVSPGVETRYFTLQKTIDV
CEEEEEEECCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHH
IHRAAPRQRIFIVCKTPQDVLTLVRGDVPIQAVNVGNMHFAEGKRQIHKTVSVDDDDIAA
HHHCCCCCEEEEEECCCHHHHHHHCCCCCEEEEECCCEEHHHHHHHHHHHCCCCCHHHHH
FRELARLGVRCEIRRVPDESGEPVDRLLD
HHHHHHCCCEEEEEECCCCCCCHHHHHCC
>Mature Secondary Structure
MITCWYMARGCDLGQFPEREFIAGGQRSGRRRSDTAKFNGYGVSPGVETRYFTLQKTIDV
CEEEEEEECCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHH
IHRAAPRQRIFIVCKTPQDVLTLVRGDVPIQAVNVGNMHFAEGKRQIHKTVSVDDDDIAA
HHHCCCCCEEEEEECCCHHHHHHHCCCCCEEEEECCCEEHHHHHHHHHHHCCCCCHHHHH
FRELARLGVRCEIRRVPDESGEPVDRLLD
HHHHHHCCCEEEEEECCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 8932697 [H]