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The map label for this gene is yieH [H]

Identifier: 85060399

GI number: 85060399

Start: 4147304

End: 4147666

Strand: Reverse

Name: yieH [H]

Synonym: SG2421

Alternate gene names: 85060399

Gene position: 4147666-4147304 (Counterclockwise)

Preceding gene: 85060400

Following gene: 85060380

Centisome position: 99.44

GC content: 53.17

Gene sequence:

>363_bases
ATGACCGATATTTCCTACGTATTATTTGACTGTGATGGCACGTTGGTTGACAGCGAGACTCTCTGCTGCGAAGCCTACAC
CGTGGTGTGTGCCCTCTATGGCATTGATATCAGCCTTGAGGAGGCAATCAAGCACTTTAAAGGCATCAAGCTCTATCAGA
TCTTTGACGACTTCCGGCAGCAATATGGCCTCACGCAGCCGATTGAGGTGTTGGAACGCCAATATCGTCAGGAGGTGGCG
CGGTTGTTCGATCTCGGCCTGAAACCTATCGAAGGTATCCGCGCGCTGCTGGAGCAAATTATGGTGCCGATGGCGGGGTC
TCTAACGGGCCGGTCAGCAAAATGGAACACTCGCTGGGGCTGA

Upstream 100 bases:

>100_bases
CGGCGCCAAACACCCTGATCCCCTTTGTTTATCACCATGCCTTGAAATCGTGTAAAGTAAAGACAATATACCGCGGCACT
TCCGCAACGAGGCGGCACTC

Downstream 100 bases:

>100_bases
CGTCATTGCTGCCCTTTTTCAACGATCGGCTCTTTAGCGGTTATACTCTCGGCCGCTGGAAACCAGACCCTGCGCTCATC
CATCATGCCGCCGCGGAGAT

Product: putative hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 120; Mature: 119

Protein sequence:

>120_residues
MTDISYVLFDCDGTLVDSETLCCEAYTVVCALYGIDISLEEAIKHFKGIKLYQIFDDFRQQYGLTQPIEVLERQYRQEVA
RLFDLGLKPIEGIRALLEQIMVPMAGSLTGRSAKWNTRWG

Sequences:

>Translated_120_residues
MTDISYVLFDCDGTLVDSETLCCEAYTVVCALYGIDISLEEAIKHFKGIKLYQIFDDFRQQYGLTQPIEVLERQYRQEVA
RLFDLGLKPIEGIRALLEQIMVPMAGSLTGRSAKWNTRWG
>Mature_119_residues
TDISYVLFDCDGTLVDSETLCCEAYTVVCALYGIDISLEEAIKHFKGIKLYQIFDDFRQQYGLTQPIEVLERQYRQEVAR
LFDLGLKPIEGIRALLEQIMVPMAGSLTGRSAKWNTRWG

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=120, Percent_Identity=45.8333333333333, Blast_Score=102, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 13732; Mature: 13601

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDISYVLFDCDGTLVDSETLCCEAYTVVCALYGIDISLEEAIKHFKGIKLYQIFDDFRQ
CCCCEEEEEECCCCEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHH
QYGLTQPIEVLERQYRQEVARLFDLGLKPIEGIRALLEQIMVPMAGSLTGRSAKWNTRWG
HCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDISYVLFDCDGTLVDSETLCCEAYTVVCALYGIDISLEEAIKHFKGIKLYQIFDDFRQ
CCCEEEEEECCCCEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHH
QYGLTQPIEVLERQYRQEVARLFDLGLKPIEGIRALLEQIMVPMAGSLTGRSAKWNTRWG
HCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]