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The map label for this gene is rffA [H]

Identifier: 85060356

GI number: 85060356

Start: 4082611

End: 4083732

Strand: Reverse

Name: rffA [H]

Synonym: SG2378

Alternate gene names: 85060356

Gene position: 4083732-4082611 (Counterclockwise)

Preceding gene: 85060357

Following gene: 85060355

Centisome position: 97.9

GC content: 58.2

Gene sequence:

>1122_bases
ATGATTCCATTTAACGCGCCACCTGTGGTCGGTACCGAACTGGGCTTTATGCAGGCCGCCATGGGCAGCGGCAAGTTGTG
CGGCGACGGCGGATTTACCCAGCGCTGCCAGCAATGGCTGCAGCAGCGCTTCGGCAGCGCGAAGGTGTTGCTTACGCCTT
CTTGCACCGCCTCGCTGGAGATGGCGGCCATCCTGCTGGATATTCGTCCCGGCGATGAAGTCATTATGCCGAGCTACACT
TTCGTTTCCACCGCTAACGCCTTTGTACTGCGCGGTGCGACCATTGTGTTCGTCGATATCCGCCCGGACACCCTGAATAT
CGATGAAACGCTGATAGAGGCGGCGATTACGCTAAAAACCCGCGCCATTGTGCCGGTCCACTATGCCGGTGTAGCCTGCG
AAATGGATACTATAATGGCCATCGCCGAGCGGCATCGTTTGTGGGTCGTGGAGGATGCCGCCCAGGGGGTCATGGCGACC
TATAAGGGGCGCGCCCTGGGCACTATCGGCCATATCGGCTGCTTTAGTTTCCACGAAACCAAAAACTATACCGCCGGTGG
TGAAGGCGGCGCGACACTTATCAATGACGCGACTCTGGTCGAGCGGGCGGAGATCATTCGCGAAAAAGGCACCAACCGCA
GCCAGTTTTTCCTCGGGCAGGTGGATAAATATACCTGGCTCGATATTGGTTCCAGTTACTTGATGGCCGATTTGCAAGCC
GCCTACTTATGGGCACAGCTGGAGGCGGCGGACACCATCAATCTACGCCGCCTGGCGCTGTGGCATCGCTACGCTTCCGC
GCTGGAACCTCTGGGCGGCCTGATGTTGCCGTCCGTTCCGGCGACTTGTCAGCACAATGCGCATATGTTTTACGTCAAAT
TGCGCGATATCGATGACCGCGACGCCTTCATCCGCCATATGAAAGCGGCGGACATCTTGACCGTGTTTCATTATATCCCG
CTACACGACAGTCCGGCGGGCCGCCGCTTTGGCCGGTTCCACGGCAGCGATCGCTACACGGGTAGCGAAAGCCGACGCCT
GGTGCGTCTACCGCTGTTTTATAATCTGAGCGACGACGATCAGCAACGCGTCATCGACGCGATACACGGCTACTACCGCT
GA

Upstream 100 bases:

>100_bases
CGGGTGGCGACGCAAACCGCCAATCTGCCGGCGCTGCGGCTTTATCTGGCAAGCGGCGCGCAGATTGTGCATGCCGCGCA
TTGGTTATACCGAGGACCTC

Downstream 100 bases:

>100_bases
TATGCCGCTGGTGAACGCCTCGGTCTGGACTGCACTGTCCACGCTGATTAAAATCGGCGCCGGCCTGCTGGTAGTAAAAG
TGTTAGCGGTGGCCTTTGGC

Product: TDP-4-oxo-6-deoxy-D-glucose transaminase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MIPFNAPPVVGTELGFMQAAMGSGKLCGDGGFTQRCQQWLQQRFGSAKVLLTPSCTASLEMAAILLDIRPGDEVIMPSYT
FVSTANAFVLRGATIVFVDIRPDTLNIDETLIEAAITLKTRAIVPVHYAGVACEMDTIMAIAERHRLWVVEDAAQGVMAT
YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDATLVERAEIIREKGTNRSQFFLGQVDKYTWLDIGSSYLMADLQA
AYLWAQLEAADTINLRRLALWHRYASALEPLGGLMLPSVPATCQHNAHMFYVKLRDIDDRDAFIRHMKAADILTVFHYIP
LHDSPAGRRFGRFHGSDRYTGSESRRLVRLPLFYNLSDDDQQRVIDAIHGYYR

Sequences:

>Translated_373_residues
MIPFNAPPVVGTELGFMQAAMGSGKLCGDGGFTQRCQQWLQQRFGSAKVLLTPSCTASLEMAAILLDIRPGDEVIMPSYT
FVSTANAFVLRGATIVFVDIRPDTLNIDETLIEAAITLKTRAIVPVHYAGVACEMDTIMAIAERHRLWVVEDAAQGVMAT
YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDATLVERAEIIREKGTNRSQFFLGQVDKYTWLDIGSSYLMADLQA
AYLWAQLEAADTINLRRLALWHRYASALEPLGGLMLPSVPATCQHNAHMFYVKLRDIDDRDAFIRHMKAADILTVFHYIP
LHDSPAGRRFGRFHGSDRYTGSESRRLVRLPLFYNLSDDDQQRVIDAIHGYYR
>Mature_373_residues
MIPFNAPPVVGTELGFMQAAMGSGKLCGDGGFTQRCQQWLQQRFGSAKVLLTPSCTASLEMAAILLDIRPGDEVIMPSYT
FVSTANAFVLRGATIVFVDIRPDTLNIDETLIEAAITLKTRAIVPVHYAGVACEMDTIMAIAERHRLWVVEDAAQGVMAT
YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDATLVERAEIIREKGTNRSQFFLGQVDKYTWLDIGSSYLMADLQA
AYLWAQLEAADTINLRRLALWHRYASALEPLGGLMLPSVPATCQHNAHMFYVKLRDIDDRDAFIRHMKAADILTVFHYIP
LHDSPAGRRFGRFHGSDRYTGSESRRLVRLPLFYNLSDDDQQRVIDAIHGYYR

Specific function: Involved in ECA elongation [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=375, Percent_Identity=80.2666666666667, Blast_Score=632, Evalue=0.0,
Organism=Escherichia coli, GI145693159, Length=382, Percent_Identity=34.2931937172775, Blast_Score=169, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR012749
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 41471; Mature: 41471

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPFNAPPVVGTELGFMQAAMGSGKLCGDGGFTQRCQQWLQQRFGSAKVLLTPSCTASLE
CCCCCCCCCCCCHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHE
MAAILLDIRPGDEVIMPSYTFVSTANAFVLRGATIVFVDIRPDTLNIDETLIEAAITLKT
EEEEEEEECCCCCEEECCCHHHHCCCEEEEECCEEEEEEECCCCCCHHHHHHHHHHHEEE
RAIVPVHYAGVACEMDTIMAIAERHRLWVVEDAAQGVMATYKGRALGTIGHIGCFSFHET
EEEEEEEECCEEECHHHHHHHHHHCCEEEEEHHHCCHHHHCCCCEEECCHHHEEEEEECC
KNYTAGGEGGATLINDATLVERAEIIREKGTNRSQFFLGQVDKYTWLDIGSSYLMADLQA
CCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCHHHHHHHHH
AYLWAQLEAADTINLRRLALWHRYASALEPLGGLMLPSVPATCQHNAHMFYVKLRDIDDR
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCH
DAFIRHMKAADILTVFHYIPLHDSPAGRRFGRFHGSDRYTGSESRRLVRLPLFYNLSDDD
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCH
QQRVIDAIHGYYR
HHHHHHHHHHCCC
>Mature Secondary Structure
MIPFNAPPVVGTELGFMQAAMGSGKLCGDGGFTQRCQQWLQQRFGSAKVLLTPSCTASLE
CCCCCCCCCCCCHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHE
MAAILLDIRPGDEVIMPSYTFVSTANAFVLRGATIVFVDIRPDTLNIDETLIEAAITLKT
EEEEEEEECCCCCEEECCCHHHHCCCEEEEECCEEEEEEECCCCCCHHHHHHHHHHHEEE
RAIVPVHYAGVACEMDTIMAIAERHRLWVVEDAAQGVMATYKGRALGTIGHIGCFSFHET
EEEEEEEECCEEECHHHHHHHHHHCCEEEEEHHHCCHHHHCCCCEEECCHHHEEEEEECC
KNYTAGGEGGATLINDATLVERAEIIREKGTNRSQFFLGQVDKYTWLDIGSSYLMADLQA
CCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCHHHHHHHHH
AYLWAQLEAADTINLRRLALWHRYASALEPLGGLMLPSVPATCQHNAHMFYVKLRDIDDR
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCH
DAFIRHMKAADILTVFHYIPLHDSPAGRRFGRFHGSDRYTGSESRRLVRLPLFYNLSDDD
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCH
QQRVIDAIHGYYR
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 8366065 [H]