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The map label for this gene is galE [C]

Identifier: 85060351

GI number: 85060351

Start: 4076268

End: 4076822

Strand: Reverse

Name: galE [C]

Synonym: SG2373

Alternate gene names: 85060351

Gene position: 4076822-4076268 (Counterclockwise)

Preceding gene: 85060352

Following gene: 85060347

Centisome position: 97.74

GC content: 43.24

Gene sequence:

>555_bases
GTGGAAACGGGTATGGAAAGGATTCTTGTTACAGGTGGAGCTGGCTACATCGGTACCCATTTAGTGAGACAGCTACTTTC
TCAGGGTCACTTTGTCCGCGTTTTGGATAATGGGACATTCGGTCTGTCAGGATTACGGCCCTTTTATGGATTGAATCGTT
TTGAGATGCAAAAAGGGGATATACAAATCACAGCGCATCTTCGCAAATCGATCAGCACTATTGATAAAATTATTCATTTG
GCTGGGATTGTAGGCAATCCGGCATGTTCCTTTGATAAAGATTTTTGTCATGAAGTTAATGTCGTTGCCACCAGACGAAT
CTTTGATGAGGCCGCCAGAGGAAATGTCAAAAACATAGTCTTTGCCTCCAGCTGTAGCGTGTACGGTTATGGTGATGATA
TTTTTACCGAAGATTCAAGATTGAATCCGTTCGATTACTACTCAGAAACCAAAGTCAAAGGTGAAGCTATCGCGCAGGCC
TACAAAGATGACTTAGTCGTGACGCTATGTCGATTCTCTACGGTTTTTGGTGCATTCACGGAGAATGCGATTTGA

Upstream 100 bases:

>100_bases
CTTACTTGACTGCAATCTAATCATATAACTACCATTAATGTTGTAGATAATGAAATGAATATTCCATCTAACGGAAATAG
GATTCGTCATTTAATGGATA

Downstream 100 bases:

>100_bases
TTTAGCGGTGAATGGTATGACCGCTAGTGCAGTCAACAGGGGAGAATACGTGATTCATGGCGGGCAACAATGGCGTCCGT
TTATTCATTGTCGGGATGTG

Product: putative sugar-nucleotide epimerase/dehydratase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
METGMERILVTGGAGYIGTHLVRQLLSQGHFVRVLDNGTFGLSGLRPFYGLNRFEMQKGDIQITAHLRKSISTIDKIIHL
AGIVGNPACSFDKDFCHEVNVVATRRIFDEAARGNVKNIVFASSCSVYGYGDDIFTEDSRLNPFDYYSETKVKGEAIAQA
YKDDLVVTLCRFSTVFGAFTENAI

Sequences:

>Translated_184_residues
METGMERILVTGGAGYIGTHLVRQLLSQGHFVRVLDNGTFGLSGLRPFYGLNRFEMQKGDIQITAHLRKSISTIDKIIHL
AGIVGNPACSFDKDFCHEVNVVATRRIFDEAARGNVKNIVFASSCSVYGYGDDIFTEDSRLNPFDYYSETKVKGEAIAQA
YKDDLVVTLCRFSTVFGAFTENAI
>Mature_184_residues
METGMERILVTGGAGYIGTHLVRQLLSQGHFVRVLDNGTFGLSGLRPFYGLNRFEMQKGDIQITAHLRKSISTIDKIIHL
AGIVGNPACSFDKDFCHEVNVVATRRIFDEAARGNVKNIVFASSCSVYGYGDDIFTEDSRLNPFDYYSETKVKGEAIAQA
YKDDLVVTLCRFSTVFGAFTENAI

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI193211614, Length=181, Percent_Identity=29.2817679558011, Blast_Score=70, Evalue=7e-13,
Organism=Homo sapiens, GI8393516, Length=181, Percent_Identity=29.2817679558011, Blast_Score=70, Evalue=7e-13,
Organism=Homo sapiens, GI42516563, Length=186, Percent_Identity=27.4193548387097, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1786974, Length=188, Percent_Identity=32.9787234042553, Blast_Score=73, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71987463, Length=176, Percent_Identity=27.8409090909091, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17539532, Length=187, Percent_Identity=28.3422459893048, Blast_Score=63, Evalue=8e-11,
Organism=Drosophila melanogaster, GI21356223, Length=182, Percent_Identity=29.1208791208791, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 20361; Mature: 20361

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METGMERILVTGGAGYIGTHLVRQLLSQGHFVRVLDNGTFGLSGLRPFYGLNRFEMQKGD
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHCCCCEEECCCC
IQITAHLRKSISTIDKIIHLAGIVGNPACSFDKDFCHEVNVVATRRIFDEAARGNVKNIV
EEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
FASSCSVYGYGDDIFTEDSRLNPFDYYSETKVKGEAIAQAYKDDLVVTLCRFSTVFGAFT
EECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENAI
CCCC
>Mature Secondary Structure
METGMERILVTGGAGYIGTHLVRQLLSQGHFVRVLDNGTFGLSGLRPFYGLNRFEMQKGD
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHCCCCEEECCCC
IQITAHLRKSISTIDKIIHLAGIVGNPACSFDKDFCHEVNVVATRRIFDEAARGNVKNIV
EEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
FASSCSVYGYGDDIFTEDSRLNPFDYYSETKVKGEAIAQAYKDDLVVTLCRFSTVFGAFT
EECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENAI
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]