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The map label for this gene is intA [H]

Identifier: 85060343

GI number: 85060343

Start: 4062484

End: 4063482

Strand: Reverse

Name: intA [H]

Synonym: SG2365

Alternate gene names: 85060343

Gene position: 4063482-4062484 (Counterclockwise)

Preceding gene: 85060347

Following gene: 85060342

Centisome position: 97.42

GC content: 43.24

Gene sequence:

>999_bases
GTGCATAACAATTCGAGTAACACTTTGGAGCAGTGCATGGGCGGAGAACTTAACAAACTCTCGGATAAGAAGCTAAGAAA
TCTATTGGGTGTACCCAGGGATAAGTACGAATTCTTTGCCGATGGTGCGGGGCTTAGCGTTAGGGTATCGACGGTAGGCG
GCATTAGCTGGACGTTCACTTATCGTAACAGTGGTACGATACAGCGTGTGACACTGGGACGTTATCCGGATATGAGTCTC
AAGAGAGCAAGGGAGAAGCGTGACGAGTGCCGCACATGGTTATCAGAAGGTAAAGACCCTCGGCGGCAATTGAAACTGAT
TAAGCAGGAATCACTGAAGCCAGTTACCGTTAGAGAAGCAATCGAGTATTGGATAAAGCATTACGCGATGGATAATAGAA
CTGATATTGGTGACCGTGTATTACAATTAAAAAAGCATATCTATCCTTACATCGGAGAGATGGCTTTATCGGACTGTGAA
ACACGCTATTGGTTGCAATGTTTCGACAGAATGAAAAAAACGGTTCCTGTTAGCGCAGGGAAGCTACTTCAATTGTGTAA
GCAGGCACTTAAATTTTGCCGCGTGCGTCGATATGCCGTCAGCAATGCTCTTGATGATTTAGTTGTATCTGATGTCGGCC
AGAAGGAATCAAAGCGCGACAGAGTCTTAACTGATGTCGAAGTTGGACAGATATGGAATGCTATAAACAACACAAATACC
TTTCAGCCTTATTATTCTGCGTGGTTGAAAATATTAATATCGTTTGGCTGTAGAGGTGCTGAGGCTAGATTGTCACGCTG
GGATGAATGGGATTTTAATAACTGGCTATGGACAGTACCTAGGGCGCATAGTAAAAGCGACACACCAATAGTAAGGCCGG
TACCAGAAAAAATGCGACCATATATGGAAAAATTAAAAGCTTGCAATTACAAAACAGGATATTTATTTGGTGAGATAAAG
CATGACGTCAGTGTCAGTTCATGGGGTATTAGAATATGA

Upstream 100 bases:

>100_bases
ATGCTTTTCATAAATCCATTGATAATCATGACTATATATTAAAATATTCTTTCTTGTTGTTTCTATGCTTTTTGATGCTT
GTGCCATTTTGCAGTAAATT

Downstream 100 bases:

>100_bases
AAGAAGTTGCAGCATGATAAACACTGGACACCTCACGACCTGCGACGGACGCTGGCAACCAAGCTCAATGACATGGGCGT
AGCTCCACATGTCGTAGAGC

Product: putative phage integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MHNNSSNTLEQCMGGELNKLSDKKLRNLLGVPRDKYEFFADGAGLSVRVSTVGGISWTFTYRNSGTIQRVTLGRYPDMSL
KRAREKRDECRTWLSEGKDPRRQLKLIKQESLKPVTVREAIEYWIKHYAMDNRTDIGDRVLQLKKHIYPYIGEMALSDCE
TRYWLQCFDRMKKTVPVSAGKLLQLCKQALKFCRVRRYAVSNALDDLVVSDVGQKESKRDRVLTDVEVGQIWNAINNTNT
FQPYYSAWLKILISFGCRGAEARLSRWDEWDFNNWLWTVPRAHSKSDTPIVRPVPEKMRPYMEKLKACNYKTGYLFGEIK
HDVSVSSWGIRI

Sequences:

>Translated_332_residues
MHNNSSNTLEQCMGGELNKLSDKKLRNLLGVPRDKYEFFADGAGLSVRVSTVGGISWTFTYRNSGTIQRVTLGRYPDMSL
KRAREKRDECRTWLSEGKDPRRQLKLIKQESLKPVTVREAIEYWIKHYAMDNRTDIGDRVLQLKKHIYPYIGEMALSDCE
TRYWLQCFDRMKKTVPVSAGKLLQLCKQALKFCRVRRYAVSNALDDLVVSDVGQKESKRDRVLTDVEVGQIWNAINNTNT
FQPYYSAWLKILISFGCRGAEARLSRWDEWDFNNWLWTVPRAHSKSDTPIVRPVPEKMRPYMEKLKACNYKTGYLFGEIK
HDVSVSSWGIRI
>Mature_332_residues
MHNNSSNTLEQCMGGELNKLSDKKLRNLLGVPRDKYEFFADGAGLSVRVSTVGGISWTFTYRNSGTIQRVTLGRYPDMSL
KRAREKRDECRTWLSEGKDPRRQLKLIKQESLKPVTVREAIEYWIKHYAMDNRTDIGDRVLQLKKHIYPYIGEMALSDCE
TRYWLQCFDRMKKTVPVSAGKLLQLCKQALKFCRVRRYAVSNALDDLVVSDVGQKESKRDRVLTDVEVGQIWNAINNTNT
FQPYYSAWLKILISFGCRGAEARLSRWDEWDFNNWLWTVPRAHSKSDTPIVRPVPEKMRPYMEKLKACNYKTGYLFGEIK
HDVSVSSWGIRI

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=317, Percent_Identity=24.9211356466877, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI145693166, Length=297, Percent_Identity=23.2323232323232, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 38624; Mature: 38624

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNNSSNTLEQCMGGELNKLSDKKLRNLLGVPRDKYEFFADGAGLSVRVSTVGGISWTFT
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCEEEEEE
YRNSGTIQRVTLGRYPDMSLKRAREKRDECRTWLSEGKDPRRQLKLIKQESLKPVTVREA
ECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHH
IEYWIKHYAMDNRTDIGDRVLQLKKHIYPYIGEMALSDCETRYWLQCFDRMKKTVPVSAG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KLLQLCKQALKFCRVRRYAVSNALDDLVVSDVGQKESKRDRVLTDVEVGQIWNAINNTNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
FQPYYSAWLKILISFGCRGAEARLSRWDEWDFNNWLWTVPRAHSKSDTPIVRPVPEKMRP
CCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHH
YMEKLKACNYKTGYLFGEIKHDVSVSSWGIRI
HHHHHHHCCCCCCCEEEHHHCCCEECCCCCCC
>Mature Secondary Structure
MHNNSSNTLEQCMGGELNKLSDKKLRNLLGVPRDKYEFFADGAGLSVRVSTVGGISWTFT
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCEEEEEE
YRNSGTIQRVTLGRYPDMSLKRAREKRDECRTWLSEGKDPRRQLKLIKQESLKPVTVREA
ECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHH
IEYWIKHYAMDNRTDIGDRVLQLKKHIYPYIGEMALSDCETRYWLQCFDRMKKTVPVSAG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KLLQLCKQALKFCRVRRYAVSNALDDLVVSDVGQKESKRDRVLTDVEVGQIWNAINNTNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
FQPYYSAWLKILISFGCRGAEARLSRWDEWDFNNWLWTVPRAHSKSDTPIVRPVPEKMRP
CCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHH
YMEKLKACNYKTGYLFGEIKHDVSVSSWGIRI
HHHHHHHCCCCCCCEEEHHHCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7511583; 9278503; 9205837; 7524073 [H]