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The map label for this gene is dam [H]

Identifier: 85060284

GI number: 85060284

Start: 3964934

End: 3965746

Strand: Reverse

Name: dam [H]

Synonym: SG2306

Alternate gene names: 85060284

Gene position: 3965746-3964934 (Counterclockwise)

Preceding gene: 85060285

Following gene: 85060283

Centisome position: 95.08

GC content: 53.75

Gene sequence:

>813_bases
ATGAAGAAGAACCGCGCATTTTTGAAATGGGCCGGCGGCAAGTATCCGCTGATTGAGGATATTCGCCGTCACCTGCCGGA
GGGTGATTGTTTAATAGAGCCTTTTGTCGGCGCCGGTTCGGTGTTTCTCAATACCGATTACGACCGATACATCCTCGCCG
ATATCAACAGTGATCTCATCAATCTGTTCAATATCGTTAAAGAGCGCCCGAACGATTTTATCCGCGATGCCCGGGCTTTA
TTTGCCGCCGACGCCAACATCTCTGAGGTGTACTACCTGCTGCGCAGCGAGTTTAATCTCTGCACGGACGCCCATCGGCG
CGCAGTGCTGTTTCTATATCTCAACCGGCATTGCTACAACGGTCTGTGCCGATATAACCTGAGAGGTGAGTTCAACGTTC
CTTTCGGCCGCTATAAAAAGCCGTACTTTCCGGAGTTGGAACTGCACCGGTTCGCCGAGAAGGCCAGTCGGGCGATGTTT
GTTTGCGAGCACTACCAACAAACGCTATCCCGCGCCGTACCGGGTTCGGTGGTATATTGTGATCCGCCTTATGTGCCGCT
CTCGACCACCGCAAACTTTACGGCATACCATACCAACAGTTTTAGTCTGACCGAGCAGAAGAACCTGGCGCGCCTCGCCA
GCAAGCTGGCTCGTGAAAGCCGGATACCGGTTCTGATTTCCAATCACGATACCGCGCTGACCCGGCAATGGTATGGCGAT
GCGGTGCTGCATCATGTCACGGCACGCCGCACCATCAGCAGCAATATTTTCGGGCGCAGCAGCGTGAATGAACTGCTGGC
GTTATATCTCTGA

Upstream 100 bases:

>100_bases
AGGACCAAAATAATTAAGCGTTCCTGAAGATAGCACTGTAGCGTTATGGTAAACAGGGTACAATCCGCGGTTCTGAAGAG
TGTGAGTAAGTAGCGAAGGC

Downstream 100 bases:

>100_bases
GCGGGGCCGCCCCGCCGCAGACTGACCGTAACTGACGGGAGACATGGATGAAAAACTATTTAATCGCTCCTTCCATCCTT
TCCGCGGATTTTGCCCGCCT

Product: DNA adenine methylase

Products: NA

Alternate protein names: DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase; M.SmaII [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MKKNRAFLKWAGGKYPLIEDIRRHLPEGDCLIEPFVGAGSVFLNTDYDRYILADINSDLINLFNIVKERPNDFIRDARAL
FAADANISEVYYLLRSEFNLCTDAHRRAVLFLYLNRHCYNGLCRYNLRGEFNVPFGRYKKPYFPELELHRFAEKASRAMF
VCEHYQQTLSRAVPGSVVYCDPPYVPLSTTANFTAYHTNSFSLTEQKNLARLASKLARESRIPVLISNHDTALTRQWYGD
AVLHHVTARRTISSNIFGRSSVNELLALYL

Sequences:

>Translated_270_residues
MKKNRAFLKWAGGKYPLIEDIRRHLPEGDCLIEPFVGAGSVFLNTDYDRYILADINSDLINLFNIVKERPNDFIRDARAL
FAADANISEVYYLLRSEFNLCTDAHRRAVLFLYLNRHCYNGLCRYNLRGEFNVPFGRYKKPYFPELELHRFAEKASRAMF
VCEHYQQTLSRAVPGSVVYCDPPYVPLSTTANFTAYHTNSFSLTEQKNLARLASKLARESRIPVLISNHDTALTRQWYGD
AVLHHVTARRTISSNIFGRSSVNELLALYL
>Mature_270_residues
MKKNRAFLKWAGGKYPLIEDIRRHLPEGDCLIEPFVGAGSVFLNTDYDRYILADINSDLINLFNIVKERPNDFIRDARAL
FAADANISEVYYLLRSEFNLCTDAHRRAVLFLYLNRHCYNGLCRYNLRGEFNVPFGRYKKPYFPELELHRFAEKASRAMF
VCEHYQQTLSRAVPGSVVYCDPPYVPLSTTANFTAYHTNSFSLTEQKNLARLASKLARESRIPVLISNHDTALTRQWYGD
AVLHHVTARRTISSNIFGRSSVNELLALYL

Specific function: Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=269, Percent_Identity=69.8884758364312, Blast_Score=391, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31144; Mature: 31144

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNRAFLKWAGGKYPLIEDIRRHLPEGDCLIEPFVGAGSVFLNTDYDRYILADINSDLI
CCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCEEEEECCCCCEEEEECCHHHH
NLFNIVKERPNDFIRDARALFAADANISEVYYLLRSEFNLCTDAHRRAVLFLYLNRHCYN
HHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHEEEEEEECHHHHC
GLCRYNLRGEFNVPFGRYKKPYFPELELHRFAEKASRAMFVCEHYQQTLSRAVPGSVVYC
CEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
DPPYVPLSTTANFTAYHTNSFSLTEQKNLARLASKLARESRIPVLISNHDTALTRQWYGD
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHH
AVLHHVTARRTISSNIFGRSSVNELLALYL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKKNRAFLKWAGGKYPLIEDIRRHLPEGDCLIEPFVGAGSVFLNTDYDRYILADINSDLI
CCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCEEEEECCCCCEEEEECCHHHH
NLFNIVKERPNDFIRDARALFAADANISEVYYLLRSEFNLCTDAHRRAVLFLYLNRHCYN
HHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHEEEEEEECHHHHC
GLCRYNLRGEFNVPFGRYKKPYFPELELHRFAEKASRAMFVCEHYQQTLSRAVPGSVVYC
CEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
DPPYVPLSTTANFTAYHTNSFSLTEQKNLARLASKLARESRIPVLISNHDTALTRQWYGD
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHH
AVLHHVTARRTISSNIFGRSSVNELLALYL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10383952 [H]