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The map label for this gene is metB [H]

Identifier: 85060141

GI number: 85060141

Start: 3770634

End: 3771794

Strand: Reverse

Name: metB [H]

Synonym: SG2163

Alternate gene names: 85060141

Gene position: 3771794-3770634 (Counterclockwise)

Preceding gene: 85060143

Following gene: 85060140

Centisome position: 90.43

GC content: 59.26

Gene sequence:

>1161_bases
ATGACGCGCAAGCAGGCCACCATCGCAGTTCGCAGCGGGTTGAATAACGACGAGCAATTCGGTTGTGTCGTTCCGCCGAT
TACCCTTTCAAGTACCTATAACTTTACCGAGTTCAACCAGCCGCGCGCCCACGACTACTCCCGGCGTGGTAATCCGACGC
GCGACGTTGTGCAGCGGGCCCTGGCGGAACTGGAGGGCGGTGCGGGTGCGGTGATGACCAGCAGCGGTATGTCGGCTATT
CATCTGGTGTGTACGGTTTTTCTCAAACCCGGCGATTTGCTGGTGGCGCCGCATGATTGTTATGGCGGCAGCTATCGGCT
GTTCGACAGTCTGAGCAAACGCGGCACCTATCGGGTGGAGTTCGTGGATCAGGCAAACCCGCAAGCGCTGGCTCAGGCGC
TGGCCGCAAAACCGAAGCTGGTGCTGATTGAAACCCCGAGCAACCCACTCTTGCGGGTTGTGGATATCGCCGCCATCTGT
CGTGAAGCGCACAAGGCGGGCGCCTTGTGCGTGGTGGATAACACCTTTATGAGTCCGGCGCTGCAGCAGCCGCTGGCGCT
GGGAGCCGATCTGGTTGTGCACTCCTGCACCAAATATCTTAACGGGCATTCCGATGTGGTCGCGGGGGCGGTGATTGCCC
GCGAGGCGGATGTGGCCATCGAACTCGCGTGGTGGGGCAACAATATTGGCGTGACCGGCAGCGCGTTCGACAGCTATCTG
CTGCTGCGCGGAATACGGACCCTCACGCCGCGTATACGGCAGCAGCAGAGTAATACCCAAGCTATCGTCGCGTATTTACA
GCAACATCCGCAGGTGAAAAAGTTGTATTATCCTGGCCTGTCGGAGAATCCGGGGCACGAAATTGCCTGTCGTCAGCAAA
GCGGCTTTGGCGCGATGCTCAGTTTTGAACTGGACAGCGACGAAACCACGCTGCGCCGATTCCTGGCCGCGTTGACGCTA
TTTACTCTGGCCGAATCGCTGGGGGGCGTGGAAAGTCTTATTTGCCACGCCGCGACCATGACGCTTGCGGGAATGGCGGC
GGAAGCCCGCGCCCGTGCCGGTATCAGTGAAACGCTGCTGCGCCTGTCGGTCGGGATTGAAGATAGCGAAGATTTAATTG
CCGATCTGGAACAGGCTTTCCAGGCGGCACTACAGAGGTAA

Upstream 100 bases:

>100_bases
TCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCTATATTTGCGCGGTTTACTCTTTCACCTCACTTTTAGC
ATTAGTCAGGATGCGACACC

Downstream 100 bases:

>100_bases
GCATGAGTCCATTAGTAAGCGTGGACAGCCCGACCGGCCGTCAATTGCATAAATTTGGCGGTAGTAGCCTTGCCGACGTG
AAATGTTACCAGCGGGTGGC

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTRKQATIAVRSGLNNDEQFGCVVPPITLSSTYNFTEFNQPRAHDYSRRGNPTRDVVQRALAELEGGAGAVMTSSGMSAI
HLVCTVFLKPGDLLVAPHDCYGGSYRLFDSLSKRGTYRVEFVDQANPQALAQALAAKPKLVLIETPSNPLLRVVDIAAIC
REAHKAGALCVVDNTFMSPALQQPLALGADLVVHSCTKYLNGHSDVVAGAVIAREADVAIELAWWGNNIGVTGSAFDSYL
LLRGIRTLTPRIRQQQSNTQAIVAYLQQHPQVKKLYYPGLSENPGHEIACRQQSGFGAMLSFELDSDETTLRRFLAALTL
FTLAESLGGVESLICHAATMTLAGMAAEARARAGISETLLRLSVGIEDSEDLIADLEQAFQAALQR

Sequences:

>Translated_386_residues
MTRKQATIAVRSGLNNDEQFGCVVPPITLSSTYNFTEFNQPRAHDYSRRGNPTRDVVQRALAELEGGAGAVMTSSGMSAI
HLVCTVFLKPGDLLVAPHDCYGGSYRLFDSLSKRGTYRVEFVDQANPQALAQALAAKPKLVLIETPSNPLLRVVDIAAIC
REAHKAGALCVVDNTFMSPALQQPLALGADLVVHSCTKYLNGHSDVVAGAVIAREADVAIELAWWGNNIGVTGSAFDSYL
LLRGIRTLTPRIRQQQSNTQAIVAYLQQHPQVKKLYYPGLSENPGHEIACRQQSGFGAMLSFELDSDETTLRRFLAALTL
FTLAESLGGVESLICHAATMTLAGMAAEARARAGISETLLRLSVGIEDSEDLIADLEQAFQAALQR
>Mature_385_residues
TRKQATIAVRSGLNNDEQFGCVVPPITLSSTYNFTEFNQPRAHDYSRRGNPTRDVVQRALAELEGGAGAVMTSSGMSAIH
LVCTVFLKPGDLLVAPHDCYGGSYRLFDSLSKRGTYRVEFVDQANPQALAQALAAKPKLVLIETPSNPLLRVVDIAAICR
EAHKAGALCVVDNTFMSPALQQPLALGADLVVHSCTKYLNGHSDVVAGAVIAREADVAIELAWWGNNIGVTGSAFDSYLL
LRGIRTLTPRIRQQQSNTQAIVAYLQQHPQVKKLYYPGLSENPGHEIACRQQSGFGAMLSFELDSDETTLRRFLAALTLF
TLAESLGGVESLICHAATMTLAGMAAEARARAGISETLLRLSVGIEDSEDLIADLEQAFQAALQR

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=381, Percent_Identity=42.7821522309711, Blast_Score=266, Evalue=4e-71,
Organism=Homo sapiens, GI299473759, Length=381, Percent_Identity=40.1574803149606, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI25453487, Length=380, Percent_Identity=37.6315789473684, Blast_Score=202, Evalue=3e-52,
Organism=Escherichia coli, GI1790375, Length=383, Percent_Identity=81.201044386423, Blast_Score=641, Evalue=0.0,
Organism=Escherichia coli, GI1789383, Length=341, Percent_Identity=31.6715542521994, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17538055, Length=367, Percent_Identity=45.7765667574932, Blast_Score=294, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI17560056, Length=365, Percent_Identity=44.1095890410959, Blast_Score=283, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI71980708, Length=369, Percent_Identity=35.230352303523, Blast_Score=217, Evalue=9e-57,
Organism=Saccharomyces cerevisiae, GI6319307, Length=388, Percent_Identity=38.659793814433, Blast_Score=246, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6321254, Length=399, Percent_Identity=34.0852130325814, Blast_Score=192, Evalue=8e-50,
Organism=Saccharomyces cerevisiae, GI6321904, Length=395, Percent_Identity=29.3670886075949, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6323333, Length=387, Percent_Identity=30.4909560723514, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6321170, Length=322, Percent_Identity=28.8819875776398, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6322590, Length=276, Percent_Identity=28.2608695652174, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322970, Length=264, Percent_Identity=26.1363636363636, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922554, Length=381, Percent_Identity=40.1574803149606, Blast_Score=284, Evalue=8e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR011821
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 41673; Mature: 41542

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKQATIAVRSGLNNDEQFGCVVPPITLSSTYNFTEFNQPRAHDYSRRGNPTRDVVQRA
CCCCHHHHHHHCCCCCCCCCCEEECCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LAELEGGAGAVMTSSGMSAIHLVCTVFLKPGDLLVAPHDCYGGSYRLFDSLSKRGTYRVE
HHHCCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCEEEE
FVDQANPQALAQALAAKPKLVLIETPSNPLLRVVDIAAICREAHKAGALCVVDNTFMSPA
EECCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHCHH
LQQPLALGADLVVHSCTKYLNGHSDVVAGAVIAREADVAIELAWWGNNIGVTGSAFDSYL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCEEEEEEEECCCCCCCCHHHHHHH
LLRGIRTLTPRIRQQQSNTQAIVAYLQQHPQVKKLYYPGLSENPGHEIACRQQSGFGAML
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCCCCEEE
SFELDSDETTLRRFLAALTLFTLAESLGGVESLICHAATMTLAGMAAEARARAGISETLL
EEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
RLSVGIEDSEDLIADLEQAFQAALQR
HHHCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRKQATIAVRSGLNNDEQFGCVVPPITLSSTYNFTEFNQPRAHDYSRRGNPTRDVVQRA
CCCHHHHHHHCCCCCCCCCCEEECCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LAELEGGAGAVMTSSGMSAIHLVCTVFLKPGDLLVAPHDCYGGSYRLFDSLSKRGTYRVE
HHHCCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCEEEE
FVDQANPQALAQALAAKPKLVLIETPSNPLLRVVDIAAICREAHKAGALCVVDNTFMSPA
EECCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHCHH
LQQPLALGADLVVHSCTKYLNGHSDVVAGAVIAREADVAIELAWWGNNIGVTGSAFDSYL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCEEEEEEEECCCCCCCCHHHHHHH
LLRGIRTLTPRIRQQQSNTQAIVAYLQQHPQVKKLYYPGLSENPGHEIACRQQSGFGAML
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCCCCEEE
SFELDSDETTLRRFLAALTLFTLAESLGGVESLICHAATMTLAGMAAEARARAGISETLL
EEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
RLSVGIEDSEDLIADLEQAFQAALQR
HHHCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6361020; 8346018; 9278503; 3307782; 9843488 [H]