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The map label for this gene is metF

Identifier: 85060140

GI number: 85060140

Start: 3767319

End: 3768242

Strand: Reverse

Name: metF

Synonym: SG2162

Alternate gene names: 85060140

Gene position: 3768242-3767319 (Counterclockwise)

Preceding gene: 85060141

Following gene: 85060138

Centisome position: 90.34

GC content: 55.3

Gene sequence:

>924_bases
ATGAGTTTCTTTCACGCCAATCAGCGGGAAGCATTGAATCAGAATCTGGCGGAACTGCAGGGCCAGATAAACGTCTCGTT
TGAGTTTTTTCCCCCTCGCACCAGCGAGATGGAACAAACTCTCTGGCAATCTATCGACCGGCTGAGCGAACTGAAACAGA
CTTTTGTCTCGGTGACCTACGGAGCCAATTCCGGCGAACGCGAACGTACCCACAGCATTATTAAAGACATCAAAGAGCGT
ACCGGTCTGGATGCCGCACCGCATCTGACCTGTATCGACGCCACCCCGCACGAGCTGCAGGCGATAGCACAGGACTATTG
GCATAGCGGGATTCGCCATATCGTGGCGTTACGTGGCGATTTACCGCCCGGCAGCGGTCGGCCGGCGATGTACGGCGCCG
ATCTGGTCGCGCTGCTGAAAAAAGTGGGCGACTTCGATATTTCCGTGGCGGCCTATCCGGAAGTGCATCCGGAAGCCAAG
AGTGCTCAATCGGATCTGATCAATCTGAAGAGGAAAGTGGATGCGGGCGCCAGCCGGGCGATTACCCAGTTCTTTTTCGA
TGTGGAAAGTTATTTGCGTTTTCGCGACCGCTGCGTTGCCGCCGGTATTGATACGGAAATTGTGCCGGGTATTTTGCCGG
TGTCCAATTTTCGCCAACTGCAACGTTTCGCCGCCGTCACCAATGTGAAAGTGCCCAACTGGATGACCACTATGTTCGAC
GGACTGGATCACGATCAGGAAACCCGCAAAATGGTGGGGGCCTCCATCGCCATGGACATGGTGAAAATCCTCAGCCGGGA
AGGGGTAAAGGATTTCCATTTTTACACCCTGAATCGCGCCGAGCTTACCTATGCCATCTGCCATACGCTGGGAGTGCGCC
CGGAGATCAAGATTGCCCCTGCGGCCAATCGGGCCGCGGGATAA

Upstream 100 bases:

>100_bases
CTCGTGAATTCAGTCATTTTCCATTTAGAAGTCTAAACGTATAGACGCTTATCTGTGGTGGGCATAATGCCTCTCCTACA
CTGTGTCATGAGGTAACGGT

Downstream 100 bases:

>100_bases
CGCTTCCCGCTGCCTGGTTTCTGATTTGCGGGCGTGACGGCCCGTTGTCGCAAGCGCCCATGTCGTTCGCGGGCGGCTTT
GCTGCCGCCGCGCGCTTCAC

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSFFHANQREALNQNLAELQGQINVSFEFFPPRTSEMEQTLWQSIDRLSELKQTFVSVTYGANSGERERTHSIIKDIKER
TGLDAAPHLTCIDATPHELQAIAQDYWHSGIRHIVALRGDLPPGSGRPAMYGADLVALLKKVGDFDISVAAYPEVHPEAK
SAQSDLINLKRKVDAGASRAITQFFFDVESYLRFRDRCVAAGIDTEIVPGILPVSNFRQLQRFAAVTNVKVPNWMTTMFD
GLDHDQETRKMVGASIAMDMVKILSREGVKDFHFYTLNRAELTYAICHTLGVRPEIKIAPAANRAAG

Sequences:

>Translated_307_residues
MSFFHANQREALNQNLAELQGQINVSFEFFPPRTSEMEQTLWQSIDRLSELKQTFVSVTYGANSGERERTHSIIKDIKER
TGLDAAPHLTCIDATPHELQAIAQDYWHSGIRHIVALRGDLPPGSGRPAMYGADLVALLKKVGDFDISVAAYPEVHPEAK
SAQSDLINLKRKVDAGASRAITQFFFDVESYLRFRDRCVAAGIDTEIVPGILPVSNFRQLQRFAAVTNVKVPNWMTTMFD
GLDHDQETRKMVGASIAMDMVKILSREGVKDFHFYTLNRAELTYAICHTLGVRPEIKIAPAANRAAG
>Mature_306_residues
SFFHANQREALNQNLAELQGQINVSFEFFPPRTSEMEQTLWQSIDRLSELKQTFVSVTYGANSGERERTHSIIKDIKERT
GLDAAPHLTCIDATPHELQAIAQDYWHSGIRHIVALRGDLPPGSGRPAMYGADLVALLKKVGDFDISVAAYPEVHPEAKS
AQSDLINLKRKVDAGASRAITQFFFDVESYLRFRDRCVAAGIDTEIVPGILPVSNFRQLQRFAAVTNVKVPNWMTTMFDG
LDHDQETRKMVGASIAMDMVKILSREGVKDFHFYTLNRAELTYAICHTLGVRPEIKIAPAANRAAG

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=303, Percent_Identity=32.6732673267327, Blast_Score=148, Evalue=7e-36,
Organism=Escherichia coli, GI1790377, Length=294, Percent_Identity=83.6734693877551, Blast_Score=533, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI25147436, Length=301, Percent_Identity=31.2292358803987, Blast_Score=149, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25147441, Length=301, Percent_Identity=31.2292358803987, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6321313, Length=300, Percent_Identity=31.6666666666667, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6325234, Length=294, Percent_Identity=32.312925170068, Blast_Score=140, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 34290; Mature: 34159

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFFHANQREALNQNLAELQGQINVSFEFFPPRTSEMEQTLWQSIDRLSELKQTFVSVTY
CCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEE
GANSGERERTHSIIKDIKERTGLDAAPHLTCIDATPHELQAIAQDYWHSGIRHIVALRGD
CCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
LPPGSGRPAMYGADLVALLKKVGDFDISVAAYPEVHPEAKSAQSDLINLKRKVDAGASRA
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHH
ITQFFFDVESYLRFRDRCVAAGIDTEIVPGILPVSNFRQLQRFAAVTNVKVPNWMTTMFD
HHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHC
GLDHDQETRKMVGASIAMDMVKILSREGVKDFHFYTLNRAELTYAICHTLGVRPEIKIAP
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECHHHHHHHHHHHHCCCCCEEECC
AANRAAG
CCCCCCC
>Mature Secondary Structure 
SFFHANQREALNQNLAELQGQINVSFEFFPPRTSEMEQTLWQSIDRLSELKQTFVSVTY
CCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEE
GANSGERERTHSIIKDIKERTGLDAAPHLTCIDATPHELQAIAQDYWHSGIRHIVALRGD
CCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
LPPGSGRPAMYGADLVALLKKVGDFDISVAAYPEVHPEAKSAQSDLINLKRKVDAGASRA
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHH
ITQFFFDVESYLRFRDRCVAAGIDTEIVPGILPVSNFRQLQRFAAVTNVKVPNWMTTMFD
HHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHC
GLDHDQETRKMVGASIAMDMVKILSREGVKDFHFYTLNRAELTYAICHTLGVRPEIKIAP
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECHHHHHHHHHHHHCCCCCEEECC
AANRAAG
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA