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The map label for this gene is spaL [H]

Identifier: 85060066

GI number: 85060066

Start: 3592573

End: 3593868

Strand: Reverse

Name: spaL [H]

Synonym: SG2088

Alternate gene names: 85060066

Gene position: 3593868-3592573 (Counterclockwise)

Preceding gene: 85060067

Following gene: 85060065

Centisome position: 86.16

GC content: 62.04

Gene sequence:

>1296_bases
ATGGAACGGCCGCTGCGAATGCTGCGACGGCAGGCGCTTCCCCTGCGTTTGTCAGGGCCTATTATTGAGGCGGCACTGCC
GGAGGTGGCGGTGGGCGAGATCTGCGAGATCCGCCGTCATTGGCGTTCGCGCGAGGTCATCGCCCACGCGCAGGTGTTGG
GGTTCCACCATGAGCGGACGGTGCTTAGCCTTATCGGCGCCGCTCATGGCCTGTCCCGGGAGATATTACTCCATCCCACC
GGCGGCCCGCTGCGGGTGGAAGTGGGCGACGCGTTGCTCGGCACCATCATCCAACCCTCGGGACGGATAAGCGAGCGCCT
GGCGCCGCCGCCGGTCCGTTGTAACAGGGAATGGCGCGCCATTGATGCGCCCGCGCCCGATTATCGACAGCGTGCAGGGG
TGCGACAGCCATTTATCACCGGTATTCGCGCGTTTGATGGGTTGTTGACCTGCGGCATCGGACAGCGAGTCGGTATTTTT
TCCTCCGCCGGCTGCGGCAAAACCATGCTTATGCATCAACTGATTGCCCAGTCCGACGCCGAGGTGTTCGTGATCGGCCT
GGTGGGTGAGAGGGGGCGCGAGGTGACCGAATTCGTCGAGACGTTGCGCCATTCCCCCCGCCGGCATCAATGTGTGCTGG
TATACGCCACCTCCAACTACGCCTCACTGGAACGATGCAATGCCGCACTGGTGGCGACAACGGTGGCCGAGTACTTTCGC
GATCAAGGCCGACGGGTTGTGCTGTTCCTCGATTCCCTGACCCGTTTTGCCCGTGCGCTGCGGGATGTGGCACTCGCCGC
GGGGGAAGCGCCGGCCAGGAGAGGTTACCCCGCTTCGGTATTCGACGCGCTACCCCGCGTTCTGGAGCGGCCGGGCAACA
GCCTCTCCGGCAGTATCACCGCGTTTTATACGGTGTTGTTGGAAGGGGATGATGAACCTGACCCGATCGCCGACGAAATC
CGCTCGATTCTCGACGGCCATATTTATCTAAGTCGTAAGCTGTCGGCGGCGAGTCACTATCCCGCTATCGATATTCTGCA
CAGCGCCAGCCGCGTGGCAGGGCAGGTGACGGATGCCCGACAGCAGGCGCTGGCCGGCGCTATGCGCGGGCTGCTGACCC
GGCTGGAAGAGATGCAGACTCTGATTGATTTAGGGGAATATCGCCTTGGCGATAATGCGGAAAATGACCGGGCGCATGCC
CAAAAACAGGCGCTATGGGATTTTCTGCGTCAAGACGCCGACCAGGCGGAGGAATTTGGCCAGACGCTGAGGTTAATGCA
TGCGTTTGATGCGTGA

Upstream 100 bases:

>100_bases
GCTACTGCAACCCTGGTGCCTGGAGAACAAGCAAAATTTCGCCAGCGCGCTTAGCGGTTTTTTCGAGCGTTTGGAACGCT
TCACCAAGGTGTTGCGCTGA

Downstream 100 bases:

>100_bases
GATTGAACGCCTTCGCGCGCATAGCGAGCAGCTCCGGCAGACATTGACGGCGACTCTTGCGGCGCTGGCGCGGCAAAGGG
AGACGCTTGCCGCACGGCTC

Product: ATP synthase SpaL

Products: NA

Alternate protein names: Invasion protein InvC [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MERPLRMLRRQALPLRLSGPIIEAALPEVAVGEICEIRRHWRSREVIAHAQVLGFHHERTVLSLIGAAHGLSREILLHPT
GGPLRVEVGDALLGTIIQPSGRISERLAPPPVRCNREWRAIDAPAPDYRQRAGVRQPFITGIRAFDGLLTCGIGQRVGIF
SSAGCGKTMLMHQLIAQSDAEVFVIGLVGERGREVTEFVETLRHSPRRHQCVLVYATSNYASLERCNAALVATTVAEYFR
DQGRRVVLFLDSLTRFARALRDVALAAGEAPARRGYPASVFDALPRVLERPGNSLSGSITAFYTVLLEGDDEPDPIADEI
RSILDGHIYLSRKLSAASHYPAIDILHSASRVAGQVTDARQQALAGAMRGLLTRLEEMQTLIDLGEYRLGDNAENDRAHA
QKQALWDFLRQDADQAEEFGQTLRLMHAFDA

Sequences:

>Translated_431_residues
MERPLRMLRRQALPLRLSGPIIEAALPEVAVGEICEIRRHWRSREVIAHAQVLGFHHERTVLSLIGAAHGLSREILLHPT
GGPLRVEVGDALLGTIIQPSGRISERLAPPPVRCNREWRAIDAPAPDYRQRAGVRQPFITGIRAFDGLLTCGIGQRVGIF
SSAGCGKTMLMHQLIAQSDAEVFVIGLVGERGREVTEFVETLRHSPRRHQCVLVYATSNYASLERCNAALVATTVAEYFR
DQGRRVVLFLDSLTRFARALRDVALAAGEAPARRGYPASVFDALPRVLERPGNSLSGSITAFYTVLLEGDDEPDPIADEI
RSILDGHIYLSRKLSAASHYPAIDILHSASRVAGQVTDARQQALAGAMRGLLTRLEEMQTLIDLGEYRLGDNAENDRAHA
QKQALWDFLRQDADQAEEFGQTLRLMHAFDA
>Mature_431_residues
MERPLRMLRRQALPLRLSGPIIEAALPEVAVGEICEIRRHWRSREVIAHAQVLGFHHERTVLSLIGAAHGLSREILLHPT
GGPLRVEVGDALLGTIIQPSGRISERLAPPPVRCNREWRAIDAPAPDYRQRAGVRQPFITGIRAFDGLLTCGIGQRVGIF
SSAGCGKTMLMHQLIAQSDAEVFVIGLVGERGREVTEFVETLRHSPRRHQCVLVYATSNYASLERCNAALVATTVAEYFR
DQGRRVVLFLDSLTRFARALRDVALAAGEAPARRGYPASVFDALPRVLERPGNSLSGSITAFYTVLLEGDDEPDPIADEI
RSILDGHIYLSRKLSAASHYPAIDILHSASRVAGQVTDARQQALAGAMRGLLTRLEEMQTLIDLGEYRLGDNAENDRAHA
QKQALWDFLRQDADQAEEFGQTLRLMHAFDA

Specific function: Necessary for efficient entry of S.typhimurium into cultured epithelial cells. Probable catalytic subunit of a protein translocase. May energize the protein export apparatus encoded in the inv locus which is required for the surface presentation of determ

COG id: COG1157

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway ATPase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATPase alpha/beta chains family [H]

Homologues:

Organism=Homo sapiens, GI32189394, Length=391, Percent_Identity=30.690537084399, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI50345984, Length=324, Percent_Identity=28.0864197530864, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI4757810, Length=324, Percent_Identity=28.0864197530864, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI19913424, Length=236, Percent_Identity=31.3559322033898, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI19913428, Length=321, Percent_Identity=27.4143302180685, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI19913426, Length=354, Percent_Identity=25.4237288135593, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI1788251, Length=416, Percent_Identity=38.4615384615385, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1790170, Length=422, Percent_Identity=30.3317535545024, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1790172, Length=380, Percent_Identity=26.3157894736842, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1790217, Length=216, Percent_Identity=29.6296296296296, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI25144756, Length=366, Percent_Identity=31.1475409836066, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71988080, Length=324, Percent_Identity=30.2469135802469, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71988063, Length=324, Percent_Identity=30.2469135802469, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17565854, Length=312, Percent_Identity=28.525641025641, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71988074, Length=313, Percent_Identity=30.0319488817891, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17570191, Length=359, Percent_Identity=27.2980501392758, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17510931, Length=329, Percent_Identity=27.9635258358663, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322581, Length=400, Percent_Identity=29.5, Blast_Score=133, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6319370, Length=326, Percent_Identity=30.3680981595092, Blast_Score=116, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6319603, Length=354, Percent_Identity=26.5536723163842, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320016, Length=287, Percent_Identity=29.2682926829268, Blast_Score=93, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24638766, Length=389, Percent_Identity=32.3907455012853, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI28574560, Length=418, Percent_Identity=30.1435406698565, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24658560, Length=324, Percent_Identity=29.320987654321, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24583988, Length=447, Percent_Identity=27.0693512304251, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24583986, Length=447, Percent_Identity=27.0693512304251, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24583984, Length=447, Percent_Identity=27.0693512304251, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24583992, Length=330, Percent_Identity=29.0909090909091, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI281361666, Length=355, Percent_Identity=25.9154929577465, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24646341, Length=355, Percent_Identity=25.9154929577465, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI17136796, Length=355, Percent_Identity=25.9154929577465, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI20129479, Length=236, Percent_Identity=31.3559322033898, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020003
- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR004100
- InterPro:   IPR005714 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF02874 ATP-synt_ab_N [H]

EC number: =3.6.3.14 [H]

Molecular weight: Translated: 47617; Mature: 47617

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS00152 ATPASE_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERPLRMLRRQALPLRLSGPIIEAALPEVAVGEICEIRRHWRSREVIAHAQVLGFHHERT
CCCHHHHHHHHCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLSLIGAAHGLSREILLHPTGGPLRVEVGDALLGTIIQPSGRISERLAPPPVRCNREWRA
HHHHHHHHCCCCCEEEEECCCCCEEEEECHHHHHHHCCCCCCHHHCCCCCCCCCCCCCEE
IDAPAPDYRQRAGVRQPFITGIRAFDGLLTCGIGQRVGIFSSAGCGKTMLMHQLIAQSDA
ECCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
EVFVIGLVGERGREVTEFVETLRHSPRRHQCVLVYATSNYASLERCNAALVATTVAEYFR
CEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHH
DQGRRVVLFLDSLTRFARALRDVALAAGEAPARRGYPASVFDALPRVLERPGNSLSGSIT
HCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCE
AFYTVLLEGDDEPDPIADEIRSILDGHIYLSRKLSAASHYPAIDILHSASRVAGQVTDAR
EEEEEEECCCCCCCHHHHHHHHHHCCHHHEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
QQALAGAMRGLLTRLEEMQTLIDLGEYRLGDNAENDRAHAQKQALWDFLRQDADQAEEFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QTLRLMHAFDA
HHHHHHHHHCC
>Mature Secondary Structure
MERPLRMLRRQALPLRLSGPIIEAALPEVAVGEICEIRRHWRSREVIAHAQVLGFHHERT
CCCHHHHHHHHCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLSLIGAAHGLSREILLHPTGGPLRVEVGDALLGTIIQPSGRISERLAPPPVRCNREWRA
HHHHHHHHCCCCCEEEEECCCCCEEEEECHHHHHHHCCCCCCHHHCCCCCCCCCCCCCEE
IDAPAPDYRQRAGVRQPFITGIRAFDGLLTCGIGQRVGIFSSAGCGKTMLMHQLIAQSDA
ECCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
EVFVIGLVGERGREVTEFVETLRHSPRRHQCVLVYATSNYASLERCNAALVATTVAEYFR
CEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHH
DQGRRVVLFLDSLTRFARALRDVALAAGEAPARRGYPASVFDALPRVLERPGNSLSGSIT
HCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCE
AFYTVLLEGDDEPDPIADEIRSILDGHIYLSRKLSAASHYPAIDILHSASRVAGQVTDAR
EEEEEEECCCCCCCHHHHHHHHHHCCHHHEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
QQALAGAMRGLLTRLEEMQTLIDLGEYRLGDNAENDRAHAQKQALWDFLRQDADQAEEFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QTLRLMHAFDA
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]