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The map label for this gene is spaS [H]

Identifier: 85060059

GI number: 85060059

Start: 3587509

End: 3588567

Strand: Reverse

Name: spaS [H]

Synonym: SG2081

Alternate gene names: 85060059

Gene position: 3588567-3587509 (Counterclockwise)

Preceding gene: 85060060

Following gene: 85060058

Centisome position: 86.03

GC content: 49.95

Gene sequence:

>1059_bases
ATGAGTAATAAAACCGAGAAGCCGACGCCTAAGCGCCTGCGCGACGCTGCTAAAAAAGGGCAAGCCTTCAAATCCCGCGA
CGTCATCATTGCCTGCATGATGTTATGCGGTGTCTGTTGGCTAACCTCCTTCACCTCGCTTGAGAGCCTGATGGATCTTT
ATCGCCAAATGGTGGTCGGCAATTTTTCGTTATCGCTGACCGCCTACCGCAATGCACTGATGTTTACCGCGCTACAAATA
CTGCTGCCCATCATAGCAATGGGCATTGTGGCATCAGCGCTGCCGTTGCTGCTGCAAACCGGCTTTATGCTGGCGACCCG
GGCCTTCAAGCTCAACTTTCAGGCGCTTAACCCCACCCGCGGTATCAAGAAAATATTCAGTCTGCGCACTGCGAAAGACT
GTGTAAAGGCGCTGCTGTATTTGGCCGCTTTCGCTATAGCGTTGATGATAGTCTGGCGCACCCATCGGCAGCTCTTGTTT
GAGCAACTGTTTGCCAATCCGCAGGACATCGTCGCCATTTGGCGGCAATTGCTTATGTCGCTGGTGCTAACCTGCCTGCT
GTGTATTGTCTGTATAGTGATATTGGATATCGTAGCTGAATATTTTCTGCACCTGCGCAGTCTCAAAATGGACAAGCAAG
AGGTTAGGAGGGAGATGAAAGAGCAGGAAGGAGACCCGGAGATTAAACATCGCCGCCGCGAGGCTCATATGGAGATCTTG
TCTGAGCAGACGCAAGCCGATATTAAAGGGTCGCAGTTGATCATTGCAAATCCTACCCATCTTGCTGTGGGCATTTATCA
TAATCCCGATTTGACCCCTTTGCCGTTTATCTCGGTGCTGGAACGCAATCAACGGGCGTTGGCCGTGCGCGACTATGCGA
AAAAGGTCGGTGTATCGGTGATTGAAGATGTTCAGCTGGCCCGTCGGATATATTATACGCATCACCTATATTCTTTTGTT
AAGATTAACGAGTTGGATCAGGTACTCCGCTTATTAAGCTGGTTGCAGGATGTGGAAAATGCCGGGCGTTATGATTATGC
AAAAGAAAGTGAGGAATAG

Upstream 100 bases:

>100_bases
TATTTCGCGCCGCTGTTGCCTGAGATATTTCGTCAATATTGGCTGTTGCCCTCTCAATTGGAGATATGGCTGAGTAGCGG
GCTATCGCCTGGGGAGGAGA

Downstream 100 bases:

>100_bases
ATTTTCTTTTGCATAATAATTAAGTGCGTCATTTAGCCCCTATTTTACTCTGATAATTTATCTTACTCGATATGGCTATT
TTCAGGAGATAAACATGACT

Product: surface presentation of antigens protein SpaS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MSNKTEKPTPKRLRDAAKKGQAFKSRDVIIACMMLCGVCWLTSFTSLESLMDLYRQMVVGNFSLSLTAYRNALMFTALQI
LLPIIAMGIVASALPLLLQTGFMLATRAFKLNFQALNPTRGIKKIFSLRTAKDCVKALLYLAAFAIALMIVWRTHRQLLF
EQLFANPQDIVAIWRQLLMSLVLTCLLCIVCIVILDIVAEYFLHLRSLKMDKQEVRREMKEQEGDPEIKHRRREAHMEIL
SEQTQADIKGSQLIIANPTHLAVGIYHNPDLTPLPFISVLERNQRALAVRDYAKKVGVSVIEDVQLARRIYYTHHLYSFV
KINELDQVLRLLSWLQDVENAGRYDYAKESEE

Sequences:

>Translated_352_residues
MSNKTEKPTPKRLRDAAKKGQAFKSRDVIIACMMLCGVCWLTSFTSLESLMDLYRQMVVGNFSLSLTAYRNALMFTALQI
LLPIIAMGIVASALPLLLQTGFMLATRAFKLNFQALNPTRGIKKIFSLRTAKDCVKALLYLAAFAIALMIVWRTHRQLLF
EQLFANPQDIVAIWRQLLMSLVLTCLLCIVCIVILDIVAEYFLHLRSLKMDKQEVRREMKEQEGDPEIKHRRREAHMEIL
SEQTQADIKGSQLIIANPTHLAVGIYHNPDLTPLPFISVLERNQRALAVRDYAKKVGVSVIEDVQLARRIYYTHHLYSFV
KINELDQVLRLLSWLQDVENAGRYDYAKESEE
>Mature_351_residues
SNKTEKPTPKRLRDAAKKGQAFKSRDVIIACMMLCGVCWLTSFTSLESLMDLYRQMVVGNFSLSLTAYRNALMFTALQIL
LPIIAMGIVASALPLLLQTGFMLATRAFKLNFQALNPTRGIKKIFSLRTAKDCVKALLYLAAFAIALMIVWRTHRQLLFE
QLFANPQDIVAIWRQLLMSLVLTCLLCIVCIVILDIVAEYFLHLRSLKMDKQEVRREMKEQEGDPEIKHRRREAHMEILS
EQTQADIKGSQLIIANPTHLAVGIYHNPDLTPLPFISVLERNQRALAVRDYAKKVGVSVIEDVQLARRIYYTHHLYSFVK
INELDQVLRLLSWLQDVENAGRYDYAKESEE

Specific function: Involved in a secretory pathway responsible for the surface presentation of determinants needed for the entry of Salmonella species into mammalian cells [H]

COG id: COG1377

COG function: function code NU; Flagellar biosynthesis pathway, component FlhB

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type III secretion exporter family [H]

Homologues:

Organism=Escherichia coli, GI1788188, Length=349, Percent_Identity=28.080229226361, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006135
- InterPro:   IPR006307 [H]

Pfam domain/function: PF01312 Bac_export_2 [H]

EC number: NA

Molecular weight: Translated: 40345; Mature: 40214

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SNKTEKPTPKRLRDAAKKGQAFKSRDVIIACMMLCGVCWLTSFTSLESLMDLYRQMVVG
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NFSLSLTAYRNALMFTALQILLPIIAMGIVASALPLLLQTGFMLATRAFKLNFQALNPTR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHH
GIKKIFSLRTAKDCVKALLYLAAFAIALMIVWRTHRQLLFEQLFANPQDIVAIWRQLLMS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LVLTCLLCIVCIVILDIVAEYFLHLRSLKMDKQEVRREMKEQEGDPEIKHRRREAHMEIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
SEQTQADIKGSQLIIANPTHLAVGIYHNPDLTPLPFISVLERNQRALAVRDYAKKVGVSV
HHHHHHCCCCCEEEEECCCEEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
IEDVQLARRIYYTHHLYSFVKINELDQVLRLLSWLQDVENAGRYDYAKESEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8404849; 11677609 [H]