Click here to switch to the map view.

The map label for this gene is trmB

Identifier: 85060012

GI number: 85060012

Start: 3500972

End: 3501691

Strand: Reverse

Name: trmB

Synonym: SG2034

Alternate gene names: 85060012

Gene position: 3501691-3500972 (Counterclockwise)

Preceding gene: 85060018

Following gene: 85060011

Centisome position: 83.95

GC content: 59.31

Gene sequence:

>720_bases
ATGAGCAACAATGTCATTTCCCCCGAATTCGACGAACAGGGACGCGCGCTGCGGCGGATCCGTAGTTTTGTCCGGCGACA
GGGCCGTCTGACCAAGGGGCAGCAGTATGCGCTGGACACGCTTTGGCCGGCAATGGGAACGGACTATCAGGCGCAGCCGC
TCGACCTTACTGCGCTGTTCGGCCGCGCGGCGCCGGTGACGCTGGAGATTGGTTTTGGCATGGGGGCGTCACTGGTTACC
ATGGCGGCCGCGCATCCCGAGCAGAATTTTATCGGAATTGAGGTGCACTTGCCGGGCGTCGGCGCCTGCCTTGCCAGCGC
GCAGGAAGCGGGCGTCAGCAACTTGCGTGTCATGTGTCACGATGCGGTCGAAGTGCTGGAGCAGATGATCCCTGATGCGT
CTTTGGCGCTGGTGCAGCTGTTTTTCCCTGACCCCTGGCATAAAGCGCGGCACAATAAACGCCGTATCATACAGGCGCCG
TTTGTCGAGCTGGTCGGACGCAAACTCGCCGCCGGTGGTGTTTTCCATATGGCGACCGATTGGCAACCTTATGCCGAACA
TATGTTGGCGGTGATGTCCGATGCCGCGTTTTTCCGTAGTCTATCCTCTACAGGCGACTATGTGCCGCGTCCGGCGAGCC
GCCCGCTAACCAAATTTGAACAGCGCGGTCAGCGATTAGGTCATGGCGTTTGGGATTTAATGTTTGCCAAAACGGTCTGA

Upstream 100 bases:

>100_bases
ACAACCTGTAAACAGGAACTTTCCTCTGTTGCGCGCTTGCTAAACAGCGCTATCTTTGCATAATGCGCGTTTGCCAACCA
CTTTCACATCGCGGAACTTT

Downstream 100 bases:

>100_bases
GCCATAGGCAGGAGCAATCCTCGTTTTCTTGACGCAGGTCATTTATCAGGAGAAGGTATGTTGCGCAAAGAAATGCTCGG
ATTATTGCTCATGACCGCCT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MSNNVISPEFDEQGRALRRIRSFVRRQGRLTKGQQYALDTLWPAMGTDYQAQPLDLTALFGRAAPVTLEIGFGMGASLVT
MAAAHPEQNFIGIEVHLPGVGACLASAQEAGVSNLRVMCHDAVEVLEQMIPDASLALVQLFFPDPWHKARHNKRRIIQAP
FVELVGRKLAAGGVFHMATDWQPYAEHMLAVMSDAAFFRSLSSTGDYVPRPASRPLTKFEQRGQRLGHGVWDLMFAKTV

Sequences:

>Translated_239_residues
MSNNVISPEFDEQGRALRRIRSFVRRQGRLTKGQQYALDTLWPAMGTDYQAQPLDLTALFGRAAPVTLEIGFGMGASLVT
MAAAHPEQNFIGIEVHLPGVGACLASAQEAGVSNLRVMCHDAVEVLEQMIPDASLALVQLFFPDPWHKARHNKRRIIQAP
FVELVGRKLAAGGVFHMATDWQPYAEHMLAVMSDAAFFRSLSSTGDYVPRPASRPLTKFEQRGQRLGHGVWDLMFAKTV
>Mature_238_residues
SNNVISPEFDEQGRALRRIRSFVRRQGRLTKGQQYALDTLWPAMGTDYQAQPLDLTALFGRAAPVTLEIGFGMGASLVTM
AAAHPEQNFIGIEVHLPGVGACLASAQEAGVSNLRVMCHDAVEVLEQMIPDASLALVQLFFPDPWHKARHNKRRIIQAPF
VELVGRKLAAGGVFHMATDWQPYAEHMLAVMSDAAFFRSLSSTGDYVPRPASRPLTKFEQRGQRLGHGVWDLMFAKTV

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=238, Percent_Identity=75.2100840336134, Blast_Score=385, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_SODGM (Q2NRB6)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455714.1
- ProteinModelPortal:   Q2NRB6
- SMR:   Q2NRB6
- STRING:   Q2NRB6
- GeneID:   3866745
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG2034
- NMPDR:   fig|343509.6.peg.4381
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- PhylomeDB:   Q2NRB6
- ProtClustDB:   PRK00121
- BioCyc:   SGLO343509:SG2034-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 26429; Mature: 26298

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: BINDING 69-69 BINDING 94-94 BINDING 121-121 BINDING 144-144 BINDING 148-148 BINDING 180-180

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNVISPEFDEQGRALRRIRSFVRRQGRLTKGQQYALDTLWPAMGTDYQAQPLDLTALF
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHH
GRAAPVTLEIGFGMGASLVTMAAAHPEQNFIGIEVHLPGVGACLASAQEAGVSNLRVMCH
CCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCHHHHHHHHH
DAVEVLEQMIPDASLALVQLFFPDPWHKARHNKRRIIQAPFVELVGRKLAAGGVFHMATD
HHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEECCC
WQPYAEHMLAVMSDAAFFRSLSSTGDYVPRPASRPLTKFEQRGQRLGHGVWDLMFAKTV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
SNNVISPEFDEQGRALRRIRSFVRRQGRLTKGQQYALDTLWPAMGTDYQAQPLDLTALF
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHH
GRAAPVTLEIGFGMGASLVTMAAAHPEQNFIGIEVHLPGVGACLASAQEAGVSNLRVMCH
CCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCHHHHHHHHH
DAVEVLEQMIPDASLALVQLFFPDPWHKARHNKRRIIQAPFVELVGRKLAAGGVFHMATD
HHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEECCC
WQPYAEHMLAVMSDAAFFRSLSSTGDYVPRPASRPLTKFEQRGQRLGHGVWDLMFAKTV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA