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The map label for this gene is prfB [H]

Identifier: 85059968

GI number: 85059968

Start: 3445796

End: 3446665

Strand: Reverse

Name: prfB [H]

Synonym: SG1990

Alternate gene names: 85059968

Gene position: 3446665-3445796 (Counterclockwise)

Preceding gene: 85059969

Following gene: 85059967

Centisome position: 82.63

GC content: 57.24

Gene sequence:

>870_bases
TTGGAAGACGTCGCTGGCCTGTTGGAGCTGGCGGTGGGAGAGGACGACGAAGAGACCTTTCTCGAAACCCAGGCTGAATT
GGACGGTCTGGAGGCCAAGCTGGGCCAGCTGGAATTCCGCCGTATGTTCTCCGGCGAATACGACAGCGCCGACTGCTATG
TCGATATCCAGGCCGGGTCCGGCGGTACCGAAGCGCAGGACTGGGCCAGCATGCTGCTCAGAATGTATTTGCGCTGGGCG
GAATCGCGCGGTTTCAAAACCGAAATTATCGAAGAGTCCGAAGGGGAAGTGGCCGGCATCAAATCGGTGACCATCAAGGT
CATCGGCGACTATGCCTATGGCTGGCTGCGGACCGAAACCGGCGTGCATCGCCTGGTGCGCAAGAGTCCGTTTGACTCCG
GCGGCCGGCGCCACACCTCGTTCAGCTCCGCCTTCGTTTACCCGGAAGTGGACGACGATATCGATATCGACATCAACCCG
GCGGATCTGCGCATCGATGTTTATCGCGCCTCCGGCGCCGGCGGCCAGCACGTTAACCGGACCGAATCCGCGGTGCGTAT
TACACATTTGCCGACCAATATCGTCACCCAGTGCCAAAACGACCGCTCGCAGCATAAAAACAAAGATCAGGCGATGAAAC
AGCTGAAAGCCAAGCTGTATGAATTCGAGCTGCAGAAAAAGAATGCGGAAAAGAAGGCGCTGGAAGATACCAAGTCCGAT
ATTGGCTGGGGCAGCCAGATCCGCTCATACGTGCTGGACGATTCCCGCATCAAGGATCTGCGTACCGGCGTGGAAACGCG
CAACGCCCAGTCAGTGCTGGATGGCGATCTGGACAAATTTATTGAAGCCAGTCTTAAAGTAGGGCTATGA

Upstream 100 bases:

>100_bases
GCCCGACGTCTGGAATGAGCCTGAACGCGCGCAGGCGCTGGGAAAAGAGCGCTCGTCGCTGGAAGCGATTGTTGAAACCA
TCGACCAGCTTACCCAGGGT

Downstream 100 bases:

>100_bases
GGAACCCATATGTCTGAATCACACTCACAGGACGGTGCCCCTCAGGCGCTGGATATGAATAACGAGCTGCGTTCGCGGCG
GGAAAAACTCGGCCAGTTGC

Product: peptide chain release factor RF-2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MEDVAGLLELAVGEDDEETFLETQAELDGLEAKLGQLEFRRMFSGEYDSADCYVDIQAGSGGTEAQDWASMLLRMYLRWA
ESRGFKTEIIEESEGEVAGIKSVTIKVIGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFSSAFVYPEVDDDIDIDINP
ADLRIDVYRASGAGGQHVNRTESAVRITHLPTNIVTQCQNDRSQHKNKDQAMKQLKAKLYEFELQKKNAEKKALEDTKSD
IGWGSQIRSYVLDDSRIKDLRTGVETRNAQSVLDGDLDKFIEASLKVGL

Sequences:

>Translated_289_residues
MEDVAGLLELAVGEDDEETFLETQAELDGLEAKLGQLEFRRMFSGEYDSADCYVDIQAGSGGTEAQDWASMLLRMYLRWA
ESRGFKTEIIEESEGEVAGIKSVTIKVIGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFSSAFVYPEVDDDIDIDINP
ADLRIDVYRASGAGGQHVNRTESAVRITHLPTNIVTQCQNDRSQHKNKDQAMKQLKAKLYEFELQKKNAEKKALEDTKSD
IGWGSQIRSYVLDDSRIKDLRTGVETRNAQSVLDGDLDKFIEASLKVGL
>Mature_289_residues
MEDVAGLLELAVGEDDEETFLETQAELDGLEAKLGQLEFRRMFSGEYDSADCYVDIQAGSGGTEAQDWASMLLRMYLRWA
ESRGFKTEIIEESEGEVAGIKSVTIKVIGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFSSAFVYPEVDDDIDIDINP
ADLRIDVYRASGAGGQHVNRTESAVRITHLPTNIVTQCQNDRSQHKNKDQAMKQLKAKLYEFELQKKNAEKKALEDTKSD
IGWGSQIRSYVLDDSRIKDLRTGVETRNAQSVLDGDLDKFIEASLKVGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=243, Percent_Identity=38.2716049382716, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI34577120, Length=251, Percent_Identity=33.8645418326693, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI166795305, Length=150, Percent_Identity=42, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI2367172, Length=289, Percent_Identity=89.9653979238754, Blast_Score=543, Evalue=1e-156,
Organism=Escherichia coli, GI1787462, Length=243, Percent_Identity=43.6213991769547, Blast_Score=199, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17542784, Length=239, Percent_Identity=32.2175732217573, Blast_Score=111, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=240, Percent_Identity=37.9166666666667, Blast_Score=146, Evalue=4e-36,
Organism=Drosophila melanogaster, GI19921226, Length=222, Percent_Identity=39.1891891891892, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 32398; Mature: 32398

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDVAGLLELAVGEDDEETFLETQAELDGLEAKLGQLEFRRMFSGEYDSADCYVDIQAGS
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
GGTEAQDWASMLLRMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKVIGDYAYGWLRTET
CCCCHHHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCEECHHEEEEEEEECHHHHEEECHH
GVHRLVRKSPFDSGGRRHTSFSSAFVYPEVDDDIDIDINPADLRIDVYRASGAGGQHVNR
HHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCEEEECCCCEEEEEEEECCCCCCCCCC
TESAVRITHLPTNIVTQCQNDRSQHKNKDQAMKQLKAKLYEFELQKKNAEKKALEDTKSD
CCCEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
IGWGSQIRSYVLDDSRIKDLRTGVETRNAQSVLDGDLDKFIEASLKVGL
CCHHHHHHHHHCCCHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEDVAGLLELAVGEDDEETFLETQAELDGLEAKLGQLEFRRMFSGEYDSADCYVDIQAGS
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
GGTEAQDWASMLLRMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKVIGDYAYGWLRTET
CCCCHHHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCEECHHEEEEEEEECHHHHEEECHH
GVHRLVRKSPFDSGGRRHTSFSSAFVYPEVDDDIDIDINPADLRIDVYRASGAGGQHVNR
HHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCEEEECCCCEEEEEEEECCCCCCCCCC
TESAVRITHLPTNIVTQCQNDRSQHKNKDQAMKQLKAKLYEFELQKKNAEKKALEDTKSD
CCCEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
IGWGSQIRSYVLDDSRIKDLRTGVETRNAQSVLDGDLDKFIEASLKVGL
CCHHHHHHHHHCCCHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA