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The map label for this gene is ptsP [H]

Identifier: 85059957

GI number: 85059957

Start: 3415344

End: 3417590

Strand: Reverse

Name: ptsP [H]

Synonym: SG1979

Alternate gene names: 85059957

Gene position: 3417590-3415344 (Counterclockwise)

Preceding gene: 85059958

Following gene: 85059956

Centisome position: 81.93

GC content: 59.99

Gene sequence:

>2247_bases
ATGCTCACGCGCTTGCGGGAAATCGTTGAAAAGGTCGCAGCCGCGCCGCGTCTGTCGGACTCACTTGACATTCTGGTCAA
TGAAACCTGTCAGGCGATGAATACCGAAGTCTGTTCGGTGTATTTGGCCGATAACGATCGTCGCTGCTATTACCTGATGG
CGACGCGCGGTTTGTTCAAACCGCGCGGGCGTACGATTACGCTGGCTTTTGACCAGGGGATTGTCGGACTGGTCGGGCGG
CTGGCTGAGCCGATTAACCTGGCAGACGCACATACCCATCCCAGTTTCAAATATATTCCAGCGGTGAAAGAGGAGCGCTT
CCGCTCTTTTCTCGGCGTGCCGATTATTCATCGCCGCCAGTTGCTCGGCGTACTGGTGGTGCAGCAGCGCGAGCATCGGC
AGTTCGACGAAAGTGAAGAGTCGTTCATGGTCACGCTGGCCACGCAGATGGCCGGCATTCTGCCTCAATCGCAGCTCAAC
GCGCTGTTCAGCCAATACCGTCAAACCCGTATCCGCGCCCTGGCGGCCGCGCCGGGAGTGGCCATCGCCCAAGGTTGGGT
GGACAGCAGCCAGCCCTCCCTTGATCAGGTTTATCAGGCCTCTTCTCTCGACAGCGGCCACGAGCGCGAGCGGCTGACGC
TGGCGCTGAACGAATCCGTCGCCGAGTTTTGCCGCCTCAGCAAGCGTTTCACCGCCAGCGCACAGAAAGACAGCGCGGCG
GTGTTCGACCTGTACAGCCATTTGCTCAATGACGCGCGTCTTAAGCGCGATTTGTTCGCGGAAATTGACGGCGGCGCGGT
GGCCGAGTGGGCGGTGAAGCTGGTGATTGAACGTTTCGCTGAAAAATTCGCGCTGCTGAGCGACAGTTACTTGCGCGAGC
GCGCCGGCGACTTGCGTTCCCTTGGCCAGCGACTGCTGTTCCATCTTGATGACAGCGCCCGCAATGTGATTCAGTGGCCA
TCACGTTTCGTGCTGGTGGCCGACGAGCTGAGCGCCTCGCTTCTGGCCGAGGTGCCGCAAGATAAGCTGGTGGGGGTGGT
GGTGCGCGATGGAGCCGCCAATTCCCACGCCGCGATCCTGGTGCGGGCGATGGGGATCCCGACGGTGATGGGCGTCGATA
TCCAGCCGCTGTTACTCAATCAGCGCTTGCTTATCGTCGATGGCTATCGCGGTGAGCTGCTGGTGGATCCGGAGCCAGTG
CTGGTGCAGGAGTATCAGCGTCTGGTGAGCGAAGAGCGTGAGCTTACCCGGCTGGCGGAAGATGATGTGGAACAGCCGGC
GGCGCTGAAAAGCGGCGAAAGCATTCAGGTGATGCTGAATGCCGGTTTGAGCGCGGAACATGAACAGCGCCTTGACAATC
GGGTGGATGGCATTGGCCTGTACCGCACCGAAATCCCCTTCATGTTGCAAAACGGCTTTCCCTCGGAAGAGGAGCAGGTG
GCGCAGTACCAGGGCATGCTGCAGCTGTTCCCTGGCAAATCGGTGACGCTGCGCACCCTGGATGTCGGCGCCGATAAACA
ACTGCCTTACATGCCAATAAGCGAGGAAAATCCCTGTCTGGGCTGGCGTGGAATTCGCATTACGCTCGATCAGCCGGAGA
TTTTCCTCATTCAGGTGCGCGCTATGTTGCGCGCCAATGCCGCCACCGGAAATTTGAGTATTCTGCTGCCCATGATAACC
AGCGTCGATGAGATCGATGAAGCGCGTCGTCTTATCGATCGCGCCGGGCGCGAAGTCGAGGAGGTGCTGGGTTATGCGTT
GCCGAAGCCGCGTATCGGTATTATGGTGGAGGTGCCGGCGATGGTATTCATGCTGCCGGCGCTGGTGAGCCGGGTGGATT
TTCTGTCGGTCGGCACCAACGACCTGACCCAGTATTTGCTAGCGGTGGACCGCAATAATACCCGGGTGGCGGCGATGTAC
GACAGTTTGCATCCGGCCATGCTGCAGGTGCTGCAGAAAATCATCAGCGAATCGCAGCGTCTTGGGTTCGCCTGCAGCCT
GTGCGGCGAAATGGCCGGCGATCCGATGGGGGCGTTGCTGCTGGCGGGCATGGGCTATCGTTCACTGAGCATGAACGGAC
GCAGTGTGGCGCGCATCAAATATCTGTTGCGCAATGTGGCGCTGGGCGAGGCGCAGGGCCTGGCGGAACGGGTCATCGGC
GCGCAAAACAGTATTGAAGTCCACCATCAGGTGGCGGCGTTCATGGAACGACGCGGCTTGGGCGGCCTGATTCGGGGCGG
ACGTTGA

Upstream 100 bases:

>100_bases
AATGAAGGAATTTTCCGGCGTGGTGATGCCGCTGCAGGAAACTGCAGCGCAGCGCTCCGCGTCGGCACACAGACGGAAAA
GAGGCTAAGTCACGCAGATT

Downstream 100 bases:

>100_bases
GCAGAGAATCGTTACACCTGCCGGCGATTATTTACATCCCCTGACTCTCCACGGCGCGCCGCTATGCTATGATACGCGAC
TTCACGCAGCGCGGAGTAAG

Product: fused phosphoenolpyruvate-protein phosphotransferase PtsP/GAF domain

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 748; Mature: 748

Protein sequence:

>748_residues
MLTRLREIVEKVAAAPRLSDSLDILVNETCQAMNTEVCSVYLADNDRRCYYLMATRGLFKPRGRTITLAFDQGIVGLVGR
LAEPINLADAHTHPSFKYIPAVKEERFRSFLGVPIIHRRQLLGVLVVQQREHRQFDESEESFMVTLATQMAGILPQSQLN
ALFSQYRQTRIRALAAAPGVAIAQGWVDSSQPSLDQVYQASSLDSGHERERLTLALNESVAEFCRLSKRFTASAQKDSAA
VFDLYSHLLNDARLKRDLFAEIDGGAVAEWAVKLVIERFAEKFALLSDSYLRERAGDLRSLGQRLLFHLDDSARNVIQWP
SRFVLVADELSASLLAEVPQDKLVGVVVRDGAANSHAAILVRAMGIPTVMGVDIQPLLLNQRLLIVDGYRGELLVDPEPV
LVQEYQRLVSEERELTRLAEDDVEQPAALKSGESIQVMLNAGLSAEHEQRLDNRVDGIGLYRTEIPFMLQNGFPSEEEQV
AQYQGMLQLFPGKSVTLRTLDVGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLSILLPMIT
SVDEIDEARRLIDRAGREVEEVLGYALPKPRIGIMVEVPAMVFMLPALVSRVDFLSVGTNDLTQYLLAVDRNNTRVAAMY
DSLHPAMLQVLQKIISESQRLGFACSLCGEMAGDPMGALLLAGMGYRSLSMNGRSVARIKYLLRNVALGEAQGLAERVIG
AQNSIEVHHQVAAFMERRGLGGLIRGGR

Sequences:

>Translated_748_residues
MLTRLREIVEKVAAAPRLSDSLDILVNETCQAMNTEVCSVYLADNDRRCYYLMATRGLFKPRGRTITLAFDQGIVGLVGR
LAEPINLADAHTHPSFKYIPAVKEERFRSFLGVPIIHRRQLLGVLVVQQREHRQFDESEESFMVTLATQMAGILPQSQLN
ALFSQYRQTRIRALAAAPGVAIAQGWVDSSQPSLDQVYQASSLDSGHERERLTLALNESVAEFCRLSKRFTASAQKDSAA
VFDLYSHLLNDARLKRDLFAEIDGGAVAEWAVKLVIERFAEKFALLSDSYLRERAGDLRSLGQRLLFHLDDSARNVIQWP
SRFVLVADELSASLLAEVPQDKLVGVVVRDGAANSHAAILVRAMGIPTVMGVDIQPLLLNQRLLIVDGYRGELLVDPEPV
LVQEYQRLVSEERELTRLAEDDVEQPAALKSGESIQVMLNAGLSAEHEQRLDNRVDGIGLYRTEIPFMLQNGFPSEEEQV
AQYQGMLQLFPGKSVTLRTLDVGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLSILLPMIT
SVDEIDEARRLIDRAGREVEEVLGYALPKPRIGIMVEVPAMVFMLPALVSRVDFLSVGTNDLTQYLLAVDRNNTRVAAMY
DSLHPAMLQVLQKIISESQRLGFACSLCGEMAGDPMGALLLAGMGYRSLSMNGRSVARIKYLLRNVALGEAQGLAERVIG
AQNSIEVHHQVAAFMERRGLGGLIRGGR
>Mature_748_residues
MLTRLREIVEKVAAAPRLSDSLDILVNETCQAMNTEVCSVYLADNDRRCYYLMATRGLFKPRGRTITLAFDQGIVGLVGR
LAEPINLADAHTHPSFKYIPAVKEERFRSFLGVPIIHRRQLLGVLVVQQREHRQFDESEESFMVTLATQMAGILPQSQLN
ALFSQYRQTRIRALAAAPGVAIAQGWVDSSQPSLDQVYQASSLDSGHERERLTLALNESVAEFCRLSKRFTASAQKDSAA
VFDLYSHLLNDARLKRDLFAEIDGGAVAEWAVKLVIERFAEKFALLSDSYLRERAGDLRSLGQRLLFHLDDSARNVIQWP
SRFVLVADELSASLLAEVPQDKLVGVVVRDGAANSHAAILVRAMGIPTVMGVDIQPLLLNQRLLIVDGYRGELLVDPEPV
LVQEYQRLVSEERELTRLAEDDVEQPAALKSGESIQVMLNAGLSAEHEQRLDNRVDGIGLYRTEIPFMLQNGFPSEEEQV
AQYQGMLQLFPGKSVTLRTLDVGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLSILLPMIT
SVDEIDEARRLIDRAGREVEEVLGYALPKPRIGIMVEVPAMVFMLPALVSRVDFLSVGTNDLTQYLLAVDRNNTRVAAMY
DSLHPAMLQVLQKIISESQRLGFACSLCGEMAGDPMGALLLAGMGYRSLSMNGRSVARIKYLLRNVALGEAQGLAERVIG
AQNSIEVHHQVAAFMERRGLGGLIRGGR

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=747, Percent_Identity=78.3132530120482, Blast_Score=1206, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=594, Percent_Identity=33.8383838383838, Blast_Score=301, Evalue=7e-83,
Organism=Escherichia coli, GI48994992, Length=506, Percent_Identity=33.201581027668, Blast_Score=261, Evalue=8e-71,
Organism=Escherichia coli, GI1788726, Length=465, Percent_Identity=31.3978494623656, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1787994, Length=413, Percent_Identity=25.6658595641646, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI226510935, Length=179, Percent_Identity=27.9329608938547, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 82982; Mature: 82982

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRLREIVEKVAAAPRLSDSLDILVNETCQAMNTEVCSVYLADNDRRCYYLMATRGLFK
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEEECCCCC
PRGRTITLAFDQGIVGLVGRLAEPINLADAHTHPSFKYIPAVKEERFRSFLGVPIIHRRQ
CCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCEECCCHHHHHHHHHHCCCHHHHHH
LLGVLVVQQREHRQFDESEESFMVTLATQMAGILPQSQLNALFSQYRQTRIRALAAAPGV
HHHHHHHHHHHHCCCCCCCHHEEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCH
AIAQGWVDSSQPSLDQVYQASSLDSGHERERLTLALNESVAEFCRLSKRFTASAQKDSAA
HHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHH
VFDLYSHLLNDARLKRDLFAEIDGGAVAEWAVKLVIERFAEKFALLSDSYLRERAGDLRS
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGQRLLFHLDDSARNVIQWPSRFVLVADELSASLLAEVPQDKLVGVVVRDGAANSHAAIL
HHHHHHHHCCCHHHHHHHCCCCEEEEEHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEE
VRAMGIPTVMGVDIQPLLLNQRLLIVDGYRGELLVDPEPVLVQEYQRLVSEERELTRLAE
EEECCCCEEECCCCCHHHHCCEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
DDVEQPAALKSGESIQVMLNAGLSAEHEQRLDNRVDGIGLYRTEIPFMLQNGFPSEEEQV
HHCCCCHHHCCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEECCCCHHCCCCCCCHHHHH
AQYQGMLQLFPGKSVTLRTLDVGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVR
HHHHCHHEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEHHHH
AMLRANAATGNLSILLPMITSVDEIDEARRLIDRAGREVEEVLGYALPKPRIGIMVEVPA
HHHHCCCCCCCCEEHHHHHHCHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEEEHHH
MVFMLPALVSRVDFLSVGTNDLTQYLLAVDRNNTRVAAMYDSLHPAMLQVLQKIISESQR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHH
LGFACSLCGEMAGDPMGALLLAGMGYRSLSMNGRSVARIKYLLRNVALGEAQGLAERVIG
CCHHHHHHHHHCCCCHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHC
AQNSIEVHHQVAAFMERRGLGGLIRGGR
CCCCHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLTRLREIVEKVAAAPRLSDSLDILVNETCQAMNTEVCSVYLADNDRRCYYLMATRGLFK
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEEECCCCC
PRGRTITLAFDQGIVGLVGRLAEPINLADAHTHPSFKYIPAVKEERFRSFLGVPIIHRRQ
CCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCEECCCHHHHHHHHHHCCCHHHHHH
LLGVLVVQQREHRQFDESEESFMVTLATQMAGILPQSQLNALFSQYRQTRIRALAAAPGV
HHHHHHHHHHHHCCCCCCCHHEEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCH
AIAQGWVDSSQPSLDQVYQASSLDSGHERERLTLALNESVAEFCRLSKRFTASAQKDSAA
HHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHH
VFDLYSHLLNDARLKRDLFAEIDGGAVAEWAVKLVIERFAEKFALLSDSYLRERAGDLRS
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGQRLLFHLDDSARNVIQWPSRFVLVADELSASLLAEVPQDKLVGVVVRDGAANSHAAIL
HHHHHHHHCCCHHHHHHHCCCCEEEEEHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEE
VRAMGIPTVMGVDIQPLLLNQRLLIVDGYRGELLVDPEPVLVQEYQRLVSEERELTRLAE
EEECCCCEEECCCCCHHHHCCEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
DDVEQPAALKSGESIQVMLNAGLSAEHEQRLDNRVDGIGLYRTEIPFMLQNGFPSEEEQV
HHCCCCHHHCCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEECCCCHHCCCCCCCHHHHH
AQYQGMLQLFPGKSVTLRTLDVGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVR
HHHHCHHEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEHHHH
AMLRANAATGNLSILLPMITSVDEIDEARRLIDRAGREVEEVLGYALPKPRIGIMVEVPA
HHHHCCCCCCCCEEHHHHHHCHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEEEHHH
MVFMLPALVSRVDFLSVGTNDLTQYLLAVDRNNTRVAAMYDSLHPAMLQVLQKIISESQR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHH
LGFACSLCGEMAGDPMGALLLAGMGYRSLSMNGRSVARIKYLLRNVALGEAQGLAERVIG
CCHHHHHHHHHCCCCHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHC
AQNSIEVHHQVAAFMERRGLGGLIRGGR
CCCCHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]