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The map label for this gene is gcvA [H]

Identifier: 85059934

GI number: 85059934

Start: 3364708

End: 3365664

Strand: Reverse

Name: gcvA [H]

Synonym: SG1956

Alternate gene names: 85059934

Gene position: 3365664-3364708 (Counterclockwise)

Preceding gene: 85059937

Following gene: 85059933

Centisome position: 80.69

GC content: 57.05

Gene sequence:

>957_bases
ATGTCCAAACGACTACCTCCGCTTAATGCCCTGCGGGTGTTCGACGCGGCCGCGCGCCATTTGAGCTTTACCAAAGCGGC
GGACGAGCTTTTCGTCACGCAGGCCGCCGTCAGCCATCAAATCAAATCGTTGGAAGACTTTCTCGGCCTGAAACTGTTCC
GCCGCCGCAATCGCTCGTTGCTGCTGACCGAGGAAGGAAAAATTTATTATCTTGATATAAAAGAAATTTTCTCGGTATTG
GCGGAGGCGACGCGCAAATTGCAATCGCGCAGCGCCAAAGGCGCGCTTACGGTCAGTCTGCTGCCGAGTTTTGCCATTCA
GTGGCTGGTGCCGCGCTTGTCGAGCTTTAACGCCGCCTGGCCGGGCATTGATGTCCTCATCCAGGCGGTGGATCGCGAAG
AGGATAAGCTGGCAGATGACGTGGACGTAGCCATTTTTTATGGCCGCGGTAATTGGCCTGGATTACGGGTGGAGAAGTTG
TATGCCGAATACCTGTTGCCGGTATGTGCGCCGGCTCTGTTTACCGGCGTCTATCCACTGAAAACGCCGGCGGATCTGAT
TCACCATACCCTATTACATGACGCCTCACGGCGAGAGTGGTTGGCCTACAGCCGGCAATTGGGCCTGAGCCAGCTAAATG
TGCAGCAAGGTCCGATTTTCAGCCACAGCGCCATGGTACTGCAGGCGGCGATCCATGGTCAGGGTGTGGCGCTGGCTAAT
AACGTCATGGCGCAGACGGAAATCGAGGCCGGCCGGCTGGCGTGCCCGTTCAATGACGTGCTGGAAAGCAAAAACGCGTT
TTACCTGGTCTGTCATGACAGTCAGTCGGAGGTGGGTAAAATCGCCGCTTTTCGTCAATGGATCCTGGCGCGCGCGGCCA
GCGAGCAAGAGACGTTCCGCTTTCGGTATGCCGCGTCGCAGCCCCTGGCGCCGCTGCATGGCACCCTTCATCAATAA

Upstream 100 bases:

>100_bases
CATAAATGGACAAATTATAATGCCTCGGATAAAAAACTAATAAATGATATGCCTATGTTGGCCCTATCCATGATACCGAG
TCCGGAAAAAGCGCCAGCCT

Downstream 100 bases:

>100_bases
TTATCGGAATTGTGACACATGCGTAGTCGTGGAATGCTGATTTTCGCCGCTCTGAGTGGATTTTTATTTGTGGCTATCGG
CGCCTTTGCCGCCCACAGCC

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSKRLPPLNALRVFDAAARHLSFTKAADELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLTEEGKIYYLDIKEIFSVL
AEATRKLQSRSAKGALTVSLLPSFAIQWLVPRLSSFNAAWPGIDVLIQAVDREEDKLADDVDVAIFYGRGNWPGLRVEKL
YAEYLLPVCAPALFTGVYPLKTPADLIHHTLLHDASRREWLAYSRQLGLSQLNVQQGPIFSHSAMVLQAAIHGQGVALAN
NVMAQTEIEAGRLACPFNDVLESKNAFYLVCHDSQSEVGKIAAFRQWILARAASEQETFRFRYAASQPLAPLHGTLHQ

Sequences:

>Translated_318_residues
MSKRLPPLNALRVFDAAARHLSFTKAADELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLTEEGKIYYLDIKEIFSVL
AEATRKLQSRSAKGALTVSLLPSFAIQWLVPRLSSFNAAWPGIDVLIQAVDREEDKLADDVDVAIFYGRGNWPGLRVEKL
YAEYLLPVCAPALFTGVYPLKTPADLIHHTLLHDASRREWLAYSRQLGLSQLNVQQGPIFSHSAMVLQAAIHGQGVALAN
NVMAQTEIEAGRLACPFNDVLESKNAFYLVCHDSQSEVGKIAAFRQWILARAASEQETFRFRYAASQPLAPLHGTLHQ
>Mature_317_residues
SKRLPPLNALRVFDAAARHLSFTKAADELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLTEEGKIYYLDIKEIFSVLA
EATRKLQSRSAKGALTVSLLPSFAIQWLVPRLSSFNAAWPGIDVLIQAVDREEDKLADDVDVAIFYGRGNWPGLRVEKLY
AEYLLPVCAPALFTGVYPLKTPADLIHHTLLHDASRREWLAYSRQLGLSQLNVQQGPIFSHSAMVLQAAIHGQGVALANN
VMAQTEIEAGRLACPFNDVLESKNAFYLVCHDSQSEVGKIAAFRQWILARAASEQETFRFRYAASQPLAPLHGTLHQ

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=303, Percent_Identity=88.1188118811881, Blast_Score=556, Evalue=1e-160,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=33.5664335664336, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1788706, Length=288, Percent_Identity=30.9027777777778, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI145693193, Length=297, Percent_Identity=27.2727272727273, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI157672245, Length=162, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786401, Length=285, Percent_Identity=27.3684210526316, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789639, Length=260, Percent_Identity=26.9230769230769, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081978, Length=263, Percent_Identity=28.1368821292776, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787128, Length=292, Percent_Identity=23.6301369863014, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790783, Length=263, Percent_Identity=30.7984790874525, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35508; Mature: 35377

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRLPPLNALRVFDAAARHLSFTKAADELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
LLTEEGKIYYLDIKEIFSVLAEATRKLQSRSAKGALTVSLLPSFAIQWLVPRLSSFNAAW
EEEECCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCC
PGIDVLIQAVDREEDKLADDVDVAIFYGRGNWPGLRVEKLYAEYLLPVCAPALFTGVYPL
CCHHHHHHHHCCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
KTPADLIHHTLLHDASRREWLAYSRQLGLSQLNVQQGPIFSHSAMVLQAAIHGQGVALAN
CCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHCCCCEEHHH
NVMAQTEIEAGRLACPFNDVLESKNAFYLVCHDSQSEVGKIAAFRQWILARAASEQETFR
HHHHHHCCCCCCEECCHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCHHHEE
FRYAASQPLAPLHGTLHQ
EEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKRLPPLNALRVFDAAARHLSFTKAADELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
LLTEEGKIYYLDIKEIFSVLAEATRKLQSRSAKGALTVSLLPSFAIQWLVPRLSSFNAAW
EEEECCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCC
PGIDVLIQAVDREEDKLADDVDVAIFYGRGNWPGLRVEKLYAEYLLPVCAPALFTGVYPL
CCHHHHHHHHCCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
KTPADLIHHTLLHDASRREWLAYSRQLGLSQLNVQQGPIFSHSAMVLQAAIHGQGVALAN
CCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHCCCCEEHHH
NVMAQTEIEAGRLACPFNDVLESKNAFYLVCHDSQSEVGKIAAFRQWILARAASEQETFR
HHHHHHCCCCCCEECCHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCHHHEE
FRYAASQPLAPLHGTLHQ
EEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]