Click here to switch to the map view.

The map label for this gene is trmJ

Identifier: 85059749

GI number: 85059749

Start: 3025889

End: 3026656

Strand: Reverse

Name: trmJ

Synonym: SG1771

Alternate gene names: 85059749

Gene position: 3026656-3025889 (Counterclockwise)

Preceding gene: 85059752

Following gene: 85059748

Centisome position: 72.56

GC content: 57.94

Gene sequence:

>768_bases
ATGCTGCACAATATCCGCATCGTTTTGGTTGAAACTTCTCATACCGGCAATATCGGCTCCACCGCTCGTGCGATGAAAAC
CATGGGGTTAAGTAATCTGTATCTGGTCAATCCCCTGCACAAGCCAGATGCGCAGGCTATTGCCTTATCTGCTGGCGCCA
GCGATATTATTGGCAATGCCTCCGTGGTTGATAATCTTGATCAGGCGCTGGCCGGGTGTAGCCTGGTGGTGGGCACCAGC
GCCCGCTCGCGGACGCTTCCCTGGCCGATGCTGGAGCCGCGGGAATGCGGCGTGAAAAGCATCCAGGAGGCGGCGCAGGC
CCCGGTGGCGCTTCTGTTCGGCCGGGAGCGAGTCGGCCTGACCAATGATGAGTTGCAAAAATGCCATTTCCACGTCCAGA
TCCCGGCTAATCACGAGTACAGTTCGCTTAATTTGGCGATGGCGGTGCAAATTCTGGCCTATGAAGTCCGTGTCGCCTGG
CTTGATAGTCAACGTCAGGACGCGACGGTGCAAGAGGCTTCGGTCTATCCTGTGGCGGAAGATCTTGAGCGTTTCTATCA
GCATCTGGAGCAGGTGCTAAGCGGTACTGGTTTCATTCGCCGTGCCCATCCGGGGCTGGTGATGAACAAGCTGCGCCGGT
TGTTTAATCGTGCGCGGCCGGAAAGCCAGGAATTGAATATCCTGCGCGGCATGCTGACCTCCATTGAGCACACGCAAAAG
CCGGAAACGCCAGCGCCGGATACCGGCCACGGCCGCCCGCAAGCGTGA

Upstream 100 bases:

>100_bases
TTAAAGAACTAAAACAGCGCGGAGGAGTATAGCAGAGCGTCAGCGAAAAACCTACGGCTATGCTACGATATTGCCCACAT
TTCCTCTGACTTGTAAGACT

Downstream 100 bases:

>100_bases
TGCCCTCCTACCGCCGGAAGTGCCCGTTGTCGTGATGCTTTCGTGCCTCCGGGCGCAAGAGTGCTTGCCGGCGGAAAGGG
AATAAATACTTGAGCAAATT

Product: putative RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MLHNIRIVLVETSHTGNIGSTARAMKTMGLSNLYLVNPLHKPDAQAIALSAGASDIIGNASVVDNLDQALAGCSLVVGTS
ARSRTLPWPMLEPRECGVKSIQEAAQAPVALLFGRERVGLTNDELQKCHFHVQIPANHEYSSLNLAMAVQILAYEVRVAW
LDSQRQDATVQEASVYPVAEDLERFYQHLEQVLSGTGFIRRAHPGLVMNKLRRLFNRARPESQELNILRGMLTSIEHTQK
PETPAPDTGHGRPQA

Sequences:

>Translated_255_residues
MLHNIRIVLVETSHTGNIGSTARAMKTMGLSNLYLVNPLHKPDAQAIALSAGASDIIGNASVVDNLDQALAGCSLVVGTS
ARSRTLPWPMLEPRECGVKSIQEAAQAPVALLFGRERVGLTNDELQKCHFHVQIPANHEYSSLNLAMAVQILAYEVRVAW
LDSQRQDATVQEASVYPVAEDLERFYQHLEQVLSGTGFIRRAHPGLVMNKLRRLFNRARPESQELNILRGMLTSIEHTQK
PETPAPDTGHGRPQA
>Mature_255_residues
MLHNIRIVLVETSHTGNIGSTARAMKTMGLSNLYLVNPLHKPDAQAIALSAGASDIIGNASVVDNLDQALAGCSLVVGTS
ARSRTLPWPMLEPRECGVKSIQEAAQAPVALLFGRERVGLTNDELQKCHFHVQIPANHEYSSLNLAMAVQILAYEVRVAW
LDSQRQDATVQEASVYPVAEDLERFYQHLEQVLSGTGFIRRAHPGLVMNKLRRLFNRARPESQELNILRGMLTSIEHTQK
PETPAPDTGHGRPQA

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family

Homologues:

Organism=Escherichia coli, GI1788881, Length=241, Percent_Identity=76.7634854771784, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1790865, Length=170, Percent_Identity=35.2941176470588, Blast_Score=87, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMJ_SODGM (Q2NS29)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455451.1
- ProteinModelPortal:   Q2NS29
- STRING:   Q2NS29
- GeneID:   3866930
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG1771
- NMPDR:   fig|343509.6.peg.3778
- eggNOG:   COG0565
- HOGENOM:   HBG724367
- OMA:   FGREHAG
- PhylomeDB:   Q2NS29
- ProtClustDB:   PRK15114
- BioCyc:   SGLO343509:SG1771-MONOMER
- GO:   GO:0005737
- InterPro:   IPR004384
- InterPro:   IPR001537
- PIRSF:   PIRSF004808
- TIGRFAMs:   TIGR00050

Pfam domain/function: PF00588 SpoU_methylase

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28024; Mature: 28024

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: BINDING 114-114 BINDING 134-134 BINDING 143-143

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHNIRIVLVETSHTGNIGSTARAMKTMGLSNLYLVNPLHKPDAQAIALSAGASDIIGNA
CCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCHHHCCCH
SVVDNLDQALAGCSLVVGTSARSRTLPWPMLEPRECGVKSIQEAAQAPVALLFGRERVGL
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHCCHHHHHHHHHCCEEEEECCCCCCC
TNDELQKCHFHVQIPANHEYSSLNLAMAVQILAYEVRVAWLDSQRQDATVQEASVYPVAE
CHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHCCCCCHHH
DLERFYQHLEQVLSGTGFIRRAHPGLVMNKLRRLFNRARPESQELNILRGMLTSIEHTQK
HHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
PETPAPDTGHGRPQA
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MLHNIRIVLVETSHTGNIGSTARAMKTMGLSNLYLVNPLHKPDAQAIALSAGASDIIGNA
CCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCHHHCCCH
SVVDNLDQALAGCSLVVGTSARSRTLPWPMLEPRECGVKSIQEAAQAPVALLFGRERVGL
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHCCHHHHHHHHHCCEEEEECCCCCCC
TNDELQKCHFHVQIPANHEYSSLNLAMAVQILAYEVRVAWLDSQRQDATVQEASVYPVAE
CHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHCCCCCHHH
DLERFYQHLEQVLSGTGFIRRAHPGLVMNKLRRLFNRARPESQELNILRGMLTSIEHTQK
HHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
PETPAPDTGHGRPQA
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA