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The map label for this gene is rlmN

Identifier: 85059741

GI number: 85059741

Start: 3018279

End: 3019460

Strand: Reverse

Name: rlmN

Synonym: SG1763

Alternate gene names: 85059741

Gene position: 3019460-3018279 (Counterclockwise)

Preceding gene: 85059742

Following gene: 85059740

Centisome position: 72.39

GC content: 58.04

Gene sequence:

>1182_bases
ATGTCTGAACAACTTTTGTCTGAACTTTCACCCGTGGCGGCAACGTCTCCTTCCCCGGCGCCCGCAGCCCAGCAGAAATT
GAACCTGCTTGATATGAACCGCCAGCAGCTGCGCGAGTTTTTTTCTTCGATGGGGGAAAAGCCCTTCCGCGCTGATCAGG
TAATGAAATGGATCTATCACTATTGCTGCGATGATTTCGATCAGATGACGGACATCAACAAGCATTTGCGCGCCAGGCTG
AAAGCGCTGGCGGAAATCCGCGCGCCGGAAGTCGCCGAGGAGCAGCGCTCCGCCGACGGTACCATCAAATGGGCCATCAA
GGTGGGCGACCAGCAGGTCGAGACGGTCTACATTCCCGAGGATGATCGCGCCACGCTGTGCGTCTCCTCCCAGGTCGGCT
GCGCGCTGCAATGCACTTTCTGCTCCACCGCGCAGCAGGGCTTCAACCGTAATCTCCGGGTGTCGGAAATTATCGGCCAG
GTCTGGCGCGCGGCAAAGATCATCGGCGCCGCCAAGGTCACCGGCCAGCGCCCTATCACCAATGTGGTAATGATGGGCAT
GGGCGAGCCACTGCTCAATCTCACCAACGTGGTGCCGGCGATGGAGATTATGTTGGACGATTTCGGCTTCGGGCTGTCCA
AGCGGCGCGTCACGTTGTCCACCTCCGGCGTCGTGCCGGCCCTGGAGAAGCTCGGCGACATGATAGACGTGGCGCTGGCG
ATTTCGCTCCATGCGCCAAACGATACGATTCGCGACGAAATTGTGCCGATTAACCGCAAATACAATATCGAGACTTTCCT
GTCGGCGGTGCGCTGTTATCTTGACAAGTCCAACGCCAATAAGGGCCGCGTGACCGTGGAGTACGTGATGCTCGATCACA
TCAACGACGGAACCGAGCACGCACATCAACTGGCGGCATGTTTGAAATATACGCCGTGCAAGATTAACCTTATTCCCTGG
AACCCGTTTCCCGGCGCCCCCTACGGCCGCAGTTCCAATAGCCGGGTGGATCGCTTCGCCAAGGTGCTGATGGGCTACGA
GTTCACGACCATCGTACGTAAAACGCGCGGCGACGATATCGATGCCGCCTGTGGGCAACTGGCGGGAGAGGTGATCGACC
GCACCAAGCGTACCCTGCGTAAACGTATGAACGGTGAATCGATTGAGGTAAAGGCGGTCTGA

Upstream 100 bases:

>100_bases
CCAACGTGCCCCGGATGAGGTGGTGATCCGCACTCGGCCGGGGCATTTCTTTAATGTATTTCGCGCCATAACGTGTAATA
ACGAGGCATGCAACAAAATG

Downstream 100 bases:

>100_bases
TGGCAAGCGCTGGCGCTGGCCTTTGACCCACCACGTTAACGGAAATAGCGTAACGATAAGGGAGCCGGCATGAAGCTGAT
TCGGATGGGTGGAGTCGTAA

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MSEQLLSELSPVAATSPSPAPAAQQKLNLLDMNRQQLREFFSSMGEKPFRADQVMKWIYHYCCDDFDQMTDINKHLRARL
KALAEIRAPEVAEEQRSADGTIKWAIKVGDQQVETVYIPEDDRATLCVSSQVGCALQCTFCSTAQQGFNRNLRVSEIIGQ
VWRAAKIIGAAKVTGQRPITNVVMMGMGEPLLNLTNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALEKLGDMIDVALA
ISLHAPNDTIRDEIVPINRKYNIETFLSAVRCYLDKSNANKGRVTVEYVMLDHINDGTEHAHQLAACLKYTPCKINLIPW
NPFPGAPYGRSSNSRVDRFAKVLMGYEFTTIVRKTRGDDIDAACGQLAGEVIDRTKRTLRKRMNGESIEVKAV

Sequences:

>Translated_393_residues
MSEQLLSELSPVAATSPSPAPAAQQKLNLLDMNRQQLREFFSSMGEKPFRADQVMKWIYHYCCDDFDQMTDINKHLRARL
KALAEIRAPEVAEEQRSADGTIKWAIKVGDQQVETVYIPEDDRATLCVSSQVGCALQCTFCSTAQQGFNRNLRVSEIIGQ
VWRAAKIIGAAKVTGQRPITNVVMMGMGEPLLNLTNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALEKLGDMIDVALA
ISLHAPNDTIRDEIVPINRKYNIETFLSAVRCYLDKSNANKGRVTVEYVMLDHINDGTEHAHQLAACLKYTPCKINLIPW
NPFPGAPYGRSSNSRVDRFAKVLMGYEFTTIVRKTRGDDIDAACGQLAGEVIDRTKRTLRKRMNGESIEVKAV
>Mature_392_residues
SEQLLSELSPVAATSPSPAPAAQQKLNLLDMNRQQLREFFSSMGEKPFRADQVMKWIYHYCCDDFDQMTDINKHLRARLK
ALAEIRAPEVAEEQRSADGTIKWAIKVGDQQVETVYIPEDDRATLCVSSQVGCALQCTFCSTAQQGFNRNLRVSEIIGQV
WRAAKIIGAAKVTGQRPITNVVMMGMGEPLLNLTNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALEKLGDMIDVALAI
SLHAPNDTIRDEIVPINRKYNIETFLSAVRCYLDKSNANKGRVTVEYVMLDHINDGTEHAHQLAACLKYTPCKINLIPWN
PFPGAPYGRSSNSRVDRFAKVLMGYEFTTIVRKTRGDDIDAACGQLAGEVIDRTKRTLRKRMNGESIEVKAV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=393, Percent_Identity=84.2239185750636, Blast_Score=702, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_SODGM (Q2NS37)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455443.1
- STRING:   Q2NS37
- GeneID:   3866922
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG1763
- NMPDR:   fig|343509.6.peg.3768
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   Q2NS37
- ProtClustDB:   PRK11194
- BioCyc:   SGLO343509:SG1763-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 43744; Mature: 43613

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQLLSELSPVAATSPSPAPAAQQKLNLLDMNRQQLREFFSSMGEKPFRADQVMKWIYH
CCHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
YCCDDFDQMTDINKHLRARLKALAEIRAPEVAEEQRSADGTIKWAIKVGDQQVETVYIPE
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEEEECCCEEEEEEECC
DDRATLCVSSQVGCALQCTFCSTAQQGFNRNLRVSEIIGQVWRAAKIIGAAKVTGQRPIT
CCCEEEEEECCCCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
NVVMMGMGEPLLNLTNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALEKLGDMIDVALA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHEEHHE
ISLHAPNDTIRDEIVPINRKYNIETFLSAVRCYLDKSNANKGRVTVEYVMLDHINDGTEH
EEEECCCCCHHHCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCHHH
AHQLAACLKYTPCKINLIPWNPFPGAPYGRSSNSRVDRFAKVLMGYEFTTIVRKTRGDDI
HHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCH
DAACGQLAGEVIDRTKRTLRKRMNGESIEVKAV
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure 
SEQLLSELSPVAATSPSPAPAAQQKLNLLDMNRQQLREFFSSMGEKPFRADQVMKWIYH
CHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
YCCDDFDQMTDINKHLRARLKALAEIRAPEVAEEQRSADGTIKWAIKVGDQQVETVYIPE
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEEEECCCEEEEEEECC
DDRATLCVSSQVGCALQCTFCSTAQQGFNRNLRVSEIIGQVWRAAKIIGAAKVTGQRPIT
CCCEEEEEECCCCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
NVVMMGMGEPLLNLTNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALEKLGDMIDVALA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHEEHHE
ISLHAPNDTIRDEIVPINRKYNIETFLSAVRCYLDKSNANKGRVTVEYVMLDHINDGTEH
EEEECCCCCHHHCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCHHH
AHQLAACLKYTPCKINLIPWNPFPGAPYGRSSNSRVDRFAKVLMGYEFTTIVRKTRGDDI
HHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCH
DAACGQLAGEVIDRTKRTLRKRMNGESIEVKAV
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA