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The map label for this gene is lacI [H]

Identifier: 85059730

GI number: 85059730

Start: 2999394

End: 3000203

Strand: Reverse

Name: lacI [H]

Synonym: SG1752

Alternate gene names: 85059730

Gene position: 3000203-2999394 (Counterclockwise)

Preceding gene: 85059734

Following gene: 85059727

Centisome position: 71.93

GC content: 61.23

Gene sequence:

>810_bases
GTGAAGCACAAGAACGCCACCCTTGACGATGTCGCCCGCCATGCCGGCGTTTCTTATCAGACGGTTTCCCGAGTTTTGAA
TGGATCGGCGCACGTGGCGGACGCGACGCGCGCACGGGTCAACGAGGCCATTAGAACGTTGAATTATGTGCCCAACCGGA
TGGCCCAGCAGCTGGCCGGCAAGCGGGGCTGTACCCTGGGGCTCATCACCGCCTCGCTCGGGTTCCATGCGCCGTCGCAA
ATCGCTTCCGCCATCAAGGGCGTCGCCGGTCAGCGCGGGTTTTCTGTGCTGATTGCCATGGCTGACCCACAGCACGAAAT
TGGACTGCAAGACGCGCTAAATGAGCTGAAGGCGCAGCGGGTGGAGAGGGTTATCATCAATTTGCCGCTGGCGGCGGAGA
GCGCTGCGGCAATCGTTGCCGATAATTCCGAACTCGTGTGCTTGTTCCTGGATGTTCCGACGGATGCCGGCGTGTTTCAC
GTCATGTTTAATCCTGGCGACGGCACGCGCGCAAGCGTTGATCATCTTTATCGCCTGGGCCACCGGCATTTTGCGCTGCT
GGCAGGTCCGCAAGATTCTGTCTCCGCACGCCTGCGCCTCGACAGCTGGCGCTTGCCGAGTATCATCTGCACCCGGTGGC
GTCAGCTCAGGGCGACTGGAGCGCCGCCAGTGGTTTTCGCTGTATGGTCACGATGCTCAAGGCGCAGGCGTCATTCAGCG
CCGTATTGGTCTCCAACGATCAGATGGCCCTCGGTGCGATTAGCGCGTTGCATCAGCAGCCGTTGGCGGTGCCGGAGCAC
GTTTCGGTGA

Upstream 100 bases:

>100_bases
ATATCACTTTTTCACTCCGCTTAGCGTTTGCCGTGGTTTGATTTATGATGTAAGGAGAATGCGATCTAGCCCTATATGAG
TTTGCTGCAGGAACAACATT

Downstream 100 bases:

>100_bases
TAGGCTATGATGATACCGAGGACAGCGCCTACTTCCTGCCAGCGTTGACCACCGTTTCTCAAGATTTCGACCGGCTGGGG
CAGGAGGCGGTGAACCGGCT

Product: putative lactose operon repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MKHKNATLDDVARHAGVSYQTVSRVLNGSAHVADATRARVNEAIRTLNYVPNRMAQQLAGKRGCTLGLITASLGFHAPSQ
IASAIKGVAGQRGFSVLIAMADPQHEIGLQDALNELKAQRVERVIINLPLAAESAAAIVADNSELVCLFLDVPTDAGVFH
VMFNPGDGTRASVDHLYRLGHRHFALLAGPQDSVSARLRLDSWRLPSIICTRWRQLRATGAPPVVFAVWSRCSRRRRHSA
PYWSPTIRWPSVRLARCISSRWRCRSTFR

Sequences:

>Translated_269_residues
MKHKNATLDDVARHAGVSYQTVSRVLNGSAHVADATRARVNEAIRTLNYVPNRMAQQLAGKRGCTLGLITASLGFHAPSQ
IASAIKGVAGQRGFSVLIAMADPQHEIGLQDALNELKAQRVERVIINLPLAAESAAAIVADNSELVCLFLDVPTDAGVFH
VMFNPGDGTRASVDHLYRLGHRHFALLAGPQDSVSARLRLDSWRLPSIICTRWRQLRATGAPPVVFAVWSRCSRRRRHSA
PYWSPTIRWPSVRLARCISSRWRCRSTFR
>Mature_269_residues
MKHKNATLDDVARHAGVSYQTVSRVLNGSAHVADATRARVNEAIRTLNYVPNRMAQQLAGKRGCTLGLITASLGFHAPSQ
IASAIKGVAGQRGFSVLIAMADPQHEIGLQDALNELKAQRVERVIINLPLAAESAAAIVADNSELVCLFLDVPTDAGVFH
VMFNPGDGTRASVDHLYRLGHRHFALLAGPQDSVSARLRLDSWRLPSIICTRWRQLRATGAPPVVFAVWSRCSRRRRHSA
PYWSPTIRWPSVRLARCISSRWRCRSTFR

Specific function: Repressor of the lactose operon. Binds allolactose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786540, Length=200, Percent_Identity=50.5, Blast_Score=187, Evalue=7e-49,
Organism=Escherichia coli, GI1790194, Length=202, Percent_Identity=31.1881188118812, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786268, Length=129, Percent_Identity=34.1085271317829, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787948, Length=198, Percent_Identity=27.2727272727273, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789068, Length=203, Percent_Identity=28.0788177339901, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788474, Length=201, Percent_Identity=31.3432835820896, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787580, Length=205, Percent_Identity=28.2926829268293, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 29700; Mature: 29700

Theoretical pI: Translated: 11.52; Mature: 11.52

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHKNATLDDVARHAGVSYQTVSRVLNGSAHVADATRARVNEAIRTLNYVPNRMAQQLAG
CCCCCCCHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KRGCTLGLITASLGFHAPSQIASAIKGVAGQRGFSVLIAMADPQHEIGLQDALNELKAQR
CCCCEEEEHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHCCHHHHHHHHHHHH
VERVIINLPLAAESAAAIVADNSELVCLFLDVPTDAGVFHVMFNPGDGTRASVDHLYRLG
HHHHHHCCCCCCCCCEEEEECCCCEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHC
HRHFALLAGPQDSVSARLRLDSWRLPSIICTRWRQLRATGAPPVVFAVWSRCSRRRRHSA
CCEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
PYWSPTIRWPSVRLARCISSRWRCRSTFR
CCCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKHKNATLDDVARHAGVSYQTVSRVLNGSAHVADATRARVNEAIRTLNYVPNRMAQQLAG
CCCCCCCHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KRGCTLGLITASLGFHAPSQIASAIKGVAGQRGFSVLIAMADPQHEIGLQDALNELKAQR
CCCCEEEEHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHCCHHHHHHHHHHHH
VERVIINLPLAAESAAAIVADNSELVCLFLDVPTDAGVFHVMFNPGDGTRASVDHLYRLG
HHHHHHCCCCCCCCCEEEEECCCCEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHC
HRHFALLAGPQDSVSARLRLDSWRLPSIICTRWRQLRATGAPPVVFAVWSRCSRRRRHSA
CCEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
PYWSPTIRWPSVRLARCISSRWRCRSTFR
CCCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 355891; 9278503; 1107032; 4571224; 4594037; 3286877; 7498473; 2178920; 8046748; 2040302; 1923807; 3064080; 2742823; 8683581; 10647179; 8638105 [H]