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The map label for this gene is cysP [H]

Identifier: 85059687

GI number: 85059687

Start: 2919406

End: 2920422

Strand: Reverse

Name: cysP [H]

Synonym: SG1709

Alternate gene names: 85059687

Gene position: 2920422-2919406 (Counterclockwise)

Preceding gene: 85059689

Following gene: 85059686

Centisome position: 70.01

GC content: 56.54

Gene sequence:

>1017_bases
ATGACCTTTACGCTTTTTTTCGGGCGGGCAAGACAAATTTGCGCCGTAGTGGCATTGTTGGCATACGGACAAGCACAGGC
CACCACATTGCTTAACAGCTCCTATGACGTGGCACGTGAACTTTTCGCGACCCTCAATCCCGGTTTTATCGCGCAGTGGC
AGCAGGAACATCCGGGCGACAAACTGACCCTGCGCCAGTCGCACGCCGGCTCTTCCCGGCAGGCGCTGACCATACTGCAG
GGATTTCCGGCGGATGTGGTGACCTATAATCAGGTCACCGATGTACAGATTCTCCACGATCGCGGCCAATTGATTGCCGC
CGATTGGCGTGACCGCTTACCCAATCAGAGTTCGCCTTATTACTCAACCATGGCGTTTCTGGTGCGCAAAGGAAATCCAA
AAGGGATCCATGACTGGCGCGATCTGGCCCGTGACGGCGTGCAGCTGGTGTTTCCCAATCCCAAGACCTCGGGAAACGGT
CGCTACACCTATTTAGCCGCCTGGGGCGCGATGGAGCAGGCGTTTGGTGGCGACCGCACCCGCACCTGGATGCAGAAATT
TCTGGCCAACGTCGAGGTGTTCGATACCGGCGGGCGTGGCGCAACGACGACCTTCATCGAACGCGGTCAGGGGGATGTGC
TGATTAGTTTTGAATCAGAGGTCAACACGCTGCGCGATCAGGCCGGCGCCGGCGACTACGAAGTTATCGTGCCGGACACG
GATATTCTGGCGGAATTTCCGGTGGCCTGGGTAGATAAGAACGTGGAAGAGAACGGAACCGCGCAGGTGGCGAAAGCGTA
CCTCAACTATCTCTATAGTCCGGCGGCGCAGCACATTATCGCCGGGTTTTATTATCGGGTAAACGATACACAGATGATGA
ACGTAAACAAAAGCAAATTTCCACCGACCCGGCTGTTTCGTGTTGAGGATATATTCGGCGGTTGGCATTCGGCTATGGCG
ACGCACTTCGAGCGCGGCGGCGAACTGGATCAATTATTGGCGGCGGGCCATCGCTGA

Upstream 100 bases:

>100_bases
ACCAATCGATATATAAAAGCGTTATAGGTTATCCCCTCGTTATAAATAAACTGTAAGACTCTCAGGGCGGCCTTTTTGTC
CATCAACATCAGGACACAAC

Downstream 100 bases:

>100_bases
TGCTGGTCCTGACCACCAAACGGGTCCTGCCCGGTTTTAGCCTGAGCCTCGGCGGCAGCGTGCTGTTCGTTTGCTTGATC
CTGCTGCTGCCGTTAAGCGC

Product: thiosulfate transporter subunit

Products: SO42- [Cytoplasm]; phosphate; ADP; S2O32- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MTFTLFFGRARQICAVVALLAYGQAQATTLLNSSYDVARELFATLNPGFIAQWQQEHPGDKLTLRQSHAGSSRQALTILQ
GFPADVVTYNQVTDVQILHDRGQLIAADWRDRLPNQSSPYYSTMAFLVRKGNPKGIHDWRDLARDGVQLVFPNPKTSGNG
RYTYLAAWGAMEQAFGGDRTRTWMQKFLANVEVFDTGGRGATTTFIERGQGDVLISFESEVNTLRDQAGAGDYEVIVPDT
DILAEFPVAWVDKNVEENGTAQVAKAYLNYLYSPAAQHIIAGFYYRVNDTQMMNVNKSKFPPTRLFRVEDIFGGWHSAMA
THFERGGELDQLLAAGHR

Sequences:

>Translated_338_residues
MTFTLFFGRARQICAVVALLAYGQAQATTLLNSSYDVARELFATLNPGFIAQWQQEHPGDKLTLRQSHAGSSRQALTILQ
GFPADVVTYNQVTDVQILHDRGQLIAADWRDRLPNQSSPYYSTMAFLVRKGNPKGIHDWRDLARDGVQLVFPNPKTSGNG
RYTYLAAWGAMEQAFGGDRTRTWMQKFLANVEVFDTGGRGATTTFIERGQGDVLISFESEVNTLRDQAGAGDYEVIVPDT
DILAEFPVAWVDKNVEENGTAQVAKAYLNYLYSPAAQHIIAGFYYRVNDTQMMNVNKSKFPPTRLFRVEDIFGGWHSAMA
THFERGGELDQLLAAGHR
>Mature_337_residues
TFTLFFGRARQICAVVALLAYGQAQATTLLNSSYDVARELFATLNPGFIAQWQQEHPGDKLTLRQSHAGSSRQALTILQG
FPADVVTYNQVTDVQILHDRGQLIAADWRDRLPNQSSPYYSTMAFLVRKGNPKGIHDWRDLARDGVQLVFPNPKTSGNGR
YTYLAAWGAMEQAFGGDRTRTWMQKFLANVEVFDTGGRGATTTFIERGQGDVLISFESEVNTLRDQAGAGDYEVIVPDTD
ILAEFPVAWVDKNVEENGTAQVAKAYLNYLYSPAAQHIIAGFYYRVNDTQMMNVNKSKFPPTRLFRVEDIFGGWHSAMAT
HFERGGELDQLLAAGHR

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. This protein specifically binds thiosulfate and is involved in its transmembrane transport [H]

COG id: COG4150

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1788765, Length=316, Percent_Identity=70.8860759493671, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1790351, Length=310, Percent_Identity=46.4516129032258, Blast_Score=289, Evalue=2e-79,

Paralogues:

None

Copy number: 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37744; Mature: 37613

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFTLFFGRARQICAVVALLAYGQAQATTLLNSSYDVARELFATLNPGFIAQWQQEHPGD
CEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCCCEECHHHCCCCC
KLTLRQSHAGSSRQALTILQGFPADVVTYNQVTDVQILHDRGQLIAADWRDRLPNQSSPY
EEEEEECCCCCCCCEEHHHHCCCCCEEEECCCCCEEEEECCCCEEEECHHHCCCCCCCCH
YSTMAFLVRKGNPKGIHDWRDLARDGVQLVFPNPKTSGNGRYTYLAAWGAMEQAFGGDRT
HHHHHHHHCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCEEEEEECCHHHHHHCCCHH
RTWMQKFLANVEVFDTGGRGATTTFIERGQGDVLISFESEVNTLRDQAGAGDYEVIVPDT
HHHHHHHHCCCEEEECCCCCCCHHHEECCCCCEEEEECHHHHHHHHHCCCCCEEEEECCH
DILAEFPVAWVDKNVEENGTAQVAKAYLNYLYSPAAQHIIAGFYYRVNDTQMMNVNKSKF
HHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCEEEEECCCCCC
PPTRLFRVEDIFGGWHSAMATHFERGGELDQLLAAGHR
CCHHEEEEHHHHCCHHHHHHHHHHCCCCHHHHHHCCCC
>Mature Secondary Structure 
TFTLFFGRARQICAVVALLAYGQAQATTLLNSSYDVARELFATLNPGFIAQWQQEHPGD
EEEEEEHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCCCEECHHHCCCCC
KLTLRQSHAGSSRQALTILQGFPADVVTYNQVTDVQILHDRGQLIAADWRDRLPNQSSPY
EEEEEECCCCCCCCEEHHHHCCCCCEEEECCCCCEEEEECCCCEEEECHHHCCCCCCCCH
YSTMAFLVRKGNPKGIHDWRDLARDGVQLVFPNPKTSGNGRYTYLAAWGAMEQAFGGDRT
HHHHHHHHCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCEEEEEECCHHHHHHCCCHH
RTWMQKFLANVEVFDTGGRGATTTFIERGQGDVLISFESEVNTLRDQAGAGDYEVIVPDT
HHHHHHHHCCCEEEECCCCCCCHHHEECCCCCEEEEECHHHHHHHHHCCCCCEEEEECCH
DILAEFPVAWVDKNVEENGTAQVAKAYLNYLYSPAAQHIIAGFYYRVNDTQMMNVNKSKF
HHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCEEEEECCCCCC
PPTRLFRVEDIFGGWHSAMATHFERGGELDQLLAAGHR
CCHHEEEEHHHHCCHHHHHHHHHHCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: SO42- [Periplasm]; H2O; ATP; S2O32- [Periplasm] [C]

Specific reaction: SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2188959; 9205837; 9278503; 9298646 [H]