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The map label for this gene is ligA

Identifier: 85059656

GI number: 85059656

Start: 2871309

End: 2873345

Strand: Reverse

Name: ligA

Synonym: SG1678

Alternate gene names: 85059656

Gene position: 2873345-2871309 (Counterclockwise)

Preceding gene: 85059657

Following gene: 85059654

Centisome position: 68.89

GC content: 60.82

Gene sequence:

>2037_bases
ATGGAACAGATTGAACAACATATCCTGCGGTTACGTAAGCAACTGCGACACTGGGAATACCTCTATTACGTGGAAGCCGC
CCCTGAAGTGCCCGACAGTGAATATGACCGGTTTATGGCGGAACTGCGCACGCTGGAGGCCGAACGGCCAGATCTGCTGA
CCGCCGATTCCCCCAGTCAGCGCGTCGGCGGACAAGCGCAGAGCAGTTTTGGACAGGTGCGCCATGAAGTGCCCATGCTG
TCGCTGGATAATGTTTTTGAAGAGCCGGGTTTTTTGGCGTTCGACAAGCGGGTGCGCGACAGGCTTAAGCGCGACGACGA
CATGACCTATTGCTGCGAGCTGAAGCTGGACGGTCTGGCGGTCAGCCTGCTTTATGAGAACGGCGAGCTGGTGCGGGCCG
CCACCCGCGGCGACGGCGCCACCGGTGAGGATATCACCGCCAACGTGCGCACAATCCGCACCATTCCGTTGCGTTTGCAA
GATCACGACAACTTGCCGAGGCTGCTGGAGATCCGCGGCGAAGTATTCATGTCTGAAGCGGGCTTTCTGCGACTGAATGA
GACCGCCAAACGGGAAGGGAGCAAAGTCTTCGCCAATCCGCGCAACGCCGCCGCCGGCTCCTTACGCCAGCTGGATCCCT
CGATTACCGCTAGGCGGCCGCTGACGTTTTATTGCTATGGCGTCGGCCTGCTGGAAGACGGCGAGTTGCCGGAGAGTCAT
TGGGAGCGCTTGCAGCAGTTCAAAGCCTGGGGGGTACCGGTCAGCGACCGCGTCCGCCGCTGCACCGGTAGTGCGGCGGT
GCTGGATTTTTATCGGCAGGTGCACGAGGCGCGGTTATCGCTCGGGTTCGATATCGACGGCGTCGTGATAAAAGTCGACT
CGCTGGCGCTGCAGCAGCGTCTGGGCTTTGTCGCCCGCGCGCCGCGCTGGGCTATCGCCTATAAATTTCCAGCCCAGGAG
CAGTTGACCCGGGTACGCGACGTAGAGTTTCAGGTGGGAAGGACCGGCGCAATCACGCCGGTCGCGCGCCTTGAGCCAGT
ACTGGTTTCCGGCGCCATGGTCAGTAACGCCACCCTGCACAACGCTGATGAGGTCGAGCGGCTGGGGCTGATGATTGGCG
ATACGGTCATTGTGCGTCGCGCCGGCGATGTCATCCCGCAGATTGTCGGCGTCGTCACGTCCGAACGGCCGGCTGAGGTG
CGCCCGGTAGCGTTTCCGACGCAATGCCCGGTTTGCAGCTCGGACGTCGAGCGGGTGGAAGGCGAGGCGGTGTTGCGTTG
CACCGCGGGCTTAGTTTGCGCCGCACAGCGCAAGGAAGCGCTAAAGCATTTCGTTTCCCGCCGGGCGATGGACATCGACG
GTATGGGCGATAAGATCATTGATCAGCTGGTGGAGCGGGAGCTGGTGAAAACCCCGGCCGACCTGTTCCGGCTAAATAAA
GAAATCCTGACGCGGCTGGATCGGATGGGGTCGAAATCGGCGCAAAATTTGCTGGAGGCGCTGGAAAAAGCCCGGCAGAC
CACCTTTGCGCGCTTTCTGTACGCGCTTGGGATCCGCGAGGTGGGCGAGGCCACGGCCGTCAATCTGGCTGCCGCTTACG
GCACGCTCGACGCGCTGATTGCCGCCGATATCGATTCGCTGACGGGCGTGCAGGATATTGGCAACATTGTCGCGACCCAC
GTGCGCCACTTTTTTGAGGAAACGCATAATATCGAAGTGATCCAGGATTTGTTGAGCCCGGCAATCGGTATTCGATGGCC
TGAGCCTGTGGCAGCGCCCGTCGCGGCGAGCGATAATCCCTTCGCTGGGAAAACCATTGTCCTGACCGGATCGCTAAGCA
GCCTGTCGCGGGATGAGGCCAAGGATCATCTGGTCGCGCTCGGCGCGCGGGTCAGCGGCAGCGTTTCCGCCAAAACCGAT
CTGCTCATCGCGGGGGAGGCGGCGGGGTCCAAATTGTCCAAGGCACAGCAGTTGAATATTCCGGTGATGGATGAGGCGGA
AATGATGCGTCTTCTTGGAGAATCTTCGGACGCATAA

Upstream 100 bases:

>100_bases
TATCTTGCCGGTCATCACTAACCGGAACCGGCCGGTTGCTGCTGAAATAGCGTTATTCTCAGCCCCCGCTTGCGGGGGTT
TTTGCTGCTGATGGTGCATC

Downstream 100 bases:

>100_bases
GGGGGCGAAAATGGAAAAAGAGGATCTGGTGGCTATTGCCAATACCGCGATGCCGTTTGGTAAATATCAAGGGCGGATGC
TTATCGATCTTCCCGAACCT

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 678; Mature: 678

Protein sequence:

>678_residues
MEQIEQHILRLRKQLRHWEYLYYVEAAPEVPDSEYDRFMAELRTLEAERPDLLTADSPSQRVGGQAQSSFGQVRHEVPML
SLDNVFEEPGFLAFDKRVRDRLKRDDDMTYCCELKLDGLAVSLLYENGELVRAATRGDGATGEDITANVRTIRTIPLRLQ
DHDNLPRLLEIRGEVFMSEAGFLRLNETAKREGSKVFANPRNAAAGSLRQLDPSITARRPLTFYCYGVGLLEDGELPESH
WERLQQFKAWGVPVSDRVRRCTGSAAVLDFYRQVHEARLSLGFDIDGVVIKVDSLALQQRLGFVARAPRWAIAYKFPAQE
QLTRVRDVEFQVGRTGAITPVARLEPVLVSGAMVSNATLHNADEVERLGLMIGDTVIVRRAGDVIPQIVGVVTSERPAEV
RPVAFPTQCPVCSSDVERVEGEAVLRCTAGLVCAAQRKEALKHFVSRRAMDIDGMGDKIIDQLVERELVKTPADLFRLNK
EILTRLDRMGSKSAQNLLEALEKARQTTFARFLYALGIREVGEATAVNLAAAYGTLDALIAADIDSLTGVQDIGNIVATH
VRHFFEETHNIEVIQDLLSPAIGIRWPEPVAAPVAASDNPFAGKTIVLTGSLSSLSRDEAKDHLVALGARVSGSVSAKTD
LLIAGEAAGSKLSKAQQLNIPVMDEAEMMRLLGESSDA

Sequences:

>Translated_678_residues
MEQIEQHILRLRKQLRHWEYLYYVEAAPEVPDSEYDRFMAELRTLEAERPDLLTADSPSQRVGGQAQSSFGQVRHEVPML
SLDNVFEEPGFLAFDKRVRDRLKRDDDMTYCCELKLDGLAVSLLYENGELVRAATRGDGATGEDITANVRTIRTIPLRLQ
DHDNLPRLLEIRGEVFMSEAGFLRLNETAKREGSKVFANPRNAAAGSLRQLDPSITARRPLTFYCYGVGLLEDGELPESH
WERLQQFKAWGVPVSDRVRRCTGSAAVLDFYRQVHEARLSLGFDIDGVVIKVDSLALQQRLGFVARAPRWAIAYKFPAQE
QLTRVRDVEFQVGRTGAITPVARLEPVLVSGAMVSNATLHNADEVERLGLMIGDTVIVRRAGDVIPQIVGVVTSERPAEV
RPVAFPTQCPVCSSDVERVEGEAVLRCTAGLVCAAQRKEALKHFVSRRAMDIDGMGDKIIDQLVERELVKTPADLFRLNK
EILTRLDRMGSKSAQNLLEALEKARQTTFARFLYALGIREVGEATAVNLAAAYGTLDALIAADIDSLTGVQDIGNIVATH
VRHFFEETHNIEVIQDLLSPAIGIRWPEPVAAPVAASDNPFAGKTIVLTGSLSSLSRDEAKDHLVALGARVSGSVSAKTD
LLIAGEAAGSKLSKAQQLNIPVMDEAEMMRLLGESSDA
>Mature_678_residues
MEQIEQHILRLRKQLRHWEYLYYVEAAPEVPDSEYDRFMAELRTLEAERPDLLTADSPSQRVGGQAQSSFGQVRHEVPML
SLDNVFEEPGFLAFDKRVRDRLKRDDDMTYCCELKLDGLAVSLLYENGELVRAATRGDGATGEDITANVRTIRTIPLRLQ
DHDNLPRLLEIRGEVFMSEAGFLRLNETAKREGSKVFANPRNAAAGSLRQLDPSITARRPLTFYCYGVGLLEDGELPESH
WERLQQFKAWGVPVSDRVRRCTGSAAVLDFYRQVHEARLSLGFDIDGVVIKVDSLALQQRLGFVARAPRWAIAYKFPAQE
QLTRVRDVEFQVGRTGAITPVARLEPVLVSGAMVSNATLHNADEVERLGLMIGDTVIVRRAGDVIPQIVGVVTSERPAEV
RPVAFPTQCPVCSSDVERVEGEAVLRCTAGLVCAAQRKEALKHFVSRRAMDIDGMGDKIIDQLVERELVKTPADLFRLNK
EILTRLDRMGSKSAQNLLEALEKARQTTFARFLYALGIREVGEATAVNLAAAYGTLDALIAADIDSLTGVQDIGNIVATH
VRHFFEETHNIEVIQDLLSPAIGIRWPEPVAAPVAASDNPFAGKTIVLTGSLSSLSRDEAKDHLVALGARVSGSVSAKTD
LLIAGEAAGSKLSKAQQLNIPVMDEAEMMRLLGESSDA

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=673, Percent_Identity=70.4309063893016, Blast_Score=974, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=526, Percent_Identity=24.3346007604563, Blast_Score=116, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_SODGM (Q2NSC2)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455358.1
- ProteinModelPortal:   Q2NSC2
- STRING:   Q2NSC2
- GeneID:   3868542
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG1678
- NMPDR:   fig|343509.6.peg.3580
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q2NSC2
- ProtClustDB:   PRK07956
- BioCyc:   SGLO343509:SG1678-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74813; Mature: 74813

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 115-115 BINDING 113-113 BINDING 136-136 BINDING 174-174 BINDING 291-291 BINDING 315-315

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQIEQHILRLRKQLRHWEYLYYVEAAPEVPDSEYDRFMAELRTLEAERPDLLTADSPSQ
CCHHHHHHHHHHHHHHHCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCHH
RVGGQAQSSFGQVRHEVPMLSLDNVFEEPGFLAFDKRVRDRLKRDDDMTYCCELKLDGLA
HCCCHHHHHHHHHHHHCCCEEHHHHHCCCCCEEHHHHHHHHHCCCCCCEEEEEEEECCEE
VSLLYENGELVRAATRGDGATGEDITANVRTIRTIPLRLQDHDNLPRLLEIRGEVFMSEA
EEEEECCCCEEEEECCCCCCCCCCCEECEEEEEEEEEEECCCCCCHHHHHHHHHHHCCCC
GFLRLNETAKREGSKVFANPRNAAAGSLRQLDPSITARRPLTFYCYGVGLLEDGELPESH
CCEEECHHHHHCCCEEECCCCCCCCCCHHHCCCCCCCCCCEEEEEEEEEEECCCCCCHHH
WERLQQFKAWGVPVSDRVRRCTGSAAVLDFYRQVHEARLSLGFDIDGVVIKVDSLALQQR
HHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHH
LGFVARAPRWAIAYKFPAQEQLTRVRDVEFQVGRTGAITPVARLEPVLVSGAMVSNATLH
HHHHHCCCCEEEEEECCCHHHHHHHHCCEEEECCCCCCCHHHHHCHHHEECCHHCCCCCC
NADEVERLGLMIGDTVIVRRAGDVIPQIVGVVTSERPAEVRPVAFPTQCPVCSSDVERVE
CHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHC
GEAVLRCTAGLVCAAQRKEALKHFVSRRAMDIDGMGDKIIDQLVERELVKTPADLFRLNK
CCHHEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
EILTRLDRMGSKSAQNLLEALEKARQTTFARFLYALGIREVGEATAVNLAAAYGTLDALI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AADIDSLTGVQDIGNIVATHVRHFFEETHNIEVIQDLLSPAIGIRWPEPVAAPVAASDNP
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
FAGKTIVLTGSLSSLSRDEAKDHLVALGARVSGSVSAKTDLLIAGEAAGSKLSKAQQLNI
CCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCC
PVMDEAEMMRLLGESSDA
CCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MEQIEQHILRLRKQLRHWEYLYYVEAAPEVPDSEYDRFMAELRTLEAERPDLLTADSPSQ
CCHHHHHHHHHHHHHHHCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCHH
RVGGQAQSSFGQVRHEVPMLSLDNVFEEPGFLAFDKRVRDRLKRDDDMTYCCELKLDGLA
HCCCHHHHHHHHHHHHCCCEEHHHHHCCCCCEEHHHHHHHHHCCCCCCEEEEEEEECCEE
VSLLYENGELVRAATRGDGATGEDITANVRTIRTIPLRLQDHDNLPRLLEIRGEVFMSEA
EEEEECCCCEEEEECCCCCCCCCCCEECEEEEEEEEEEECCCCCCHHHHHHHHHHHCCCC
GFLRLNETAKREGSKVFANPRNAAAGSLRQLDPSITARRPLTFYCYGVGLLEDGELPESH
CCEEECHHHHHCCCEEECCCCCCCCCCHHHCCCCCCCCCCEEEEEEEEEEECCCCCCHHH
WERLQQFKAWGVPVSDRVRRCTGSAAVLDFYRQVHEARLSLGFDIDGVVIKVDSLALQQR
HHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHH
LGFVARAPRWAIAYKFPAQEQLTRVRDVEFQVGRTGAITPVARLEPVLVSGAMVSNATLH
HHHHHCCCCEEEEEECCCHHHHHHHHCCEEEECCCCCCCHHHHHCHHHEECCHHCCCCCC
NADEVERLGLMIGDTVIVRRAGDVIPQIVGVVTSERPAEVRPVAFPTQCPVCSSDVERVE
CHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHC
GEAVLRCTAGLVCAAQRKEALKHFVSRRAMDIDGMGDKIIDQLVERELVKTPADLFRLNK
CCHHEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
EILTRLDRMGSKSAQNLLEALEKARQTTFARFLYALGIREVGEATAVNLAAAYGTLDALI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AADIDSLTGVQDIGNIVATHVRHFFEETHNIEVIQDLLSPAIGIRWPEPVAAPVAASDNP
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
FAGKTIVLTGSLSSLSRDEAKDHLVALGARVSGSVSAKTDLLIAGEAAGSKLSKAQQLNI
CCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCC
PVMDEAEMMRLLGESSDA
CCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA