Click here to switch to the map view.

The map label for this gene is gltX

Identifier: 85059654

GI number: 85059654

Start: 2857119

End: 2858540

Strand: Reverse

Name: gltX

Synonym: SG1676

Alternate gene names: 85059654

Gene position: 2858540-2857119 (Counterclockwise)

Preceding gene: 85059656

Following gene: 85059652

Centisome position: 68.53

GC content: 57.88

Gene sequence:

>1422_bases
ATGAAAATTAAAACCCGTTTCGCGCCTAGTCCCACCGGCTATTTACATGTCGGTGGCGCGCGCACCGCGCTCTATTCGTG
GCTGTTCGCCCGCCATCTGGGCGGGGAGTTTTTGCTGCGCATAGAAGACACCGATCTTGAGCGCTCAACGCAGCCGGCGA
TTGACGCCATTATGGATGGTATGAACTGGCTCAACCTGGATTGGGACGAAGGCCCCTATTTTCAGACTAAACGTTTCGAC
CGCTATAACGCGGTCATTGACGATATGCTGTCCAAGGGCACCGCCTATCACTGTTATTGCTCCAAAGAGCGGTTGGAAAC
GTTGCGCGAAGCGCAGATGGCCCGGGGCGAAAAACCGCGCTACGACGGCCGCTGCCGTGACAGCCATGAGCATCACGCCG
ACGATGAACCCTGCGTGGTGCGTTTTCGCAACCCGCAGGACGGCTCGGTGGTGTTTAATGATCTGATCCGTGGGCCTATT
GAGTTTAGCAATCAGGAGCTGGATGATCTCATCATCCGCCGCATCGACGGGTCTCCCACCTACAATTTCTGCGTGGTGGT
CGACGATTTTGATATGGCGATAACTCACGTTATCCGCGGAGAAGACCATATCAATAACACGCCGCGCCAAATCAATATCC
TCAAGGCGCTGGGCGCGCCGGTGCCGGCCTATGCCCACGTCTCGATGATCCTGGGCGATGATGGCAAAAAACTCTCCAAA
CGCCACGGCGCGGTAGGGGTCATGCAATACCGCGATGACGGTTTTTTGCCGGAAGCGCTGCTGAACTATTTGGTGCGACT
GGGATGGTCCCACGGCGATCAAGAGATTTTCAGCGTTGACGAGATGAAACAGCTCTTTAGCTTTGAGGCGGTGAGCAAGT
CCGCCAGCGCTTTCAACACCGAAAAGCTGCTGTGGCTGAATCACCATTACATTAATCATCTGCCGGCCGACTACGTCGCT
ACGCATCTGGTCTTGCATATCGAGCAGCAGGGAATTGATACCCGCACCGGCCCGCAGCTGGCGGCACTGGTGAAGCTGCT
GGGCGAGCGCTGCAAGACGCTGAAAGAGATAGCGGCCAGCTGCCGCTACTTCTACGAGGATTTCAGCGAATTCGATGCCG
ACGCGGCCAAGAAGCATTTGCGCCCGGTGGCAGTTTTGGCTCTGGAGGCGGTACGCGCCAATCTGGCGGCGCTCAGTGAA
TGGACGCCGGCCACGGTACATGCGGCTATTGAGCAAACGGCCGAGGCGCTACAGGTCGGCATGGGTAAGGTCGGTATGCC
GCTGCGCGTTGCGGTTACCGGCACCGGCCAGTCTCCCGCCCTTGACGTCACCGTGCATGCCATAGGCCAGTCGCGCTGTC
TGGCGCGAATCGTTAAGGCGTTGGACTTTATCGCCTGCCGGACGCAGGAAAGCGCCGGTTAG

Upstream 100 bases:

>100_bases
GAGGATTCGCCCCCAAGTGGTAGACTATTCCCCACTTTTTATTATGGGCCGGAAGCGCCATTCCGGCTGTTCGCCCTGCG
TAATGAAGGCAATGTAGCTG

Downstream 100 bases:

>100_bases
CCCTGATTTGCCGGCTTTTTCAGCGTTTAACCCATTTGGTGAATTTAACCGTTGACAGAGCTTGTTGGAATTCATATCAT
GCGCCCCATCCACCAGAGAC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLFARHLGGEFLLRIEDTDLERSTQPAIDAIMDGMNWLNLDWDEGPYFQTKRFD
RYNAVIDDMLSKGTAYHCYCSKERLETLREAQMARGEKPRYDGRCRDSHEHHADDEPCVVRFRNPQDGSVVFNDLIRGPI
EFSNQELDDLIIRRIDGSPTYNFCVVVDDFDMAITHVIRGEDHINNTPRQINILKALGAPVPAYAHVSMILGDDGKKLSK
RHGAVGVMQYRDDGFLPEALLNYLVRLGWSHGDQEIFSVDEMKQLFSFEAVSKSASAFNTEKLLWLNHHYINHLPADYVA
THLVLHIEQQGIDTRTGPQLAALVKLLGERCKTLKEIAASCRYFYEDFSEFDADAAKKHLRPVAVLALEAVRANLAALSE
WTPATVHAAIEQTAEALQVGMGKVGMPLRVAVTGTGQSPALDVTVHAIGQSRCLARIVKALDFIACRTQESAG

Sequences:

>Translated_473_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLFARHLGGEFLLRIEDTDLERSTQPAIDAIMDGMNWLNLDWDEGPYFQTKRFD
RYNAVIDDMLSKGTAYHCYCSKERLETLREAQMARGEKPRYDGRCRDSHEHHADDEPCVVRFRNPQDGSVVFNDLIRGPI
EFSNQELDDLIIRRIDGSPTYNFCVVVDDFDMAITHVIRGEDHINNTPRQINILKALGAPVPAYAHVSMILGDDGKKLSK
RHGAVGVMQYRDDGFLPEALLNYLVRLGWSHGDQEIFSVDEMKQLFSFEAVSKSASAFNTEKLLWLNHHYINHLPADYVA
THLVLHIEQQGIDTRTGPQLAALVKLLGERCKTLKEIAASCRYFYEDFSEFDADAAKKHLRPVAVLALEAVRANLAALSE
WTPATVHAAIEQTAEALQVGMGKVGMPLRVAVTGTGQSPALDVTVHAIGQSRCLARIVKALDFIACRTQESAG
>Mature_473_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLFARHLGGEFLLRIEDTDLERSTQPAIDAIMDGMNWLNLDWDEGPYFQTKRFD
RYNAVIDDMLSKGTAYHCYCSKERLETLREAQMARGEKPRYDGRCRDSHEHHADDEPCVVRFRNPQDGSVVFNDLIRGPI
EFSNQELDDLIIRRIDGSPTYNFCVVVDDFDMAITHVIRGEDHINNTPRQINILKALGAPVPAYAHVSMILGDDGKKLSK
RHGAVGVMQYRDDGFLPEALLNYLVRLGWSHGDQEIFSVDEMKQLFSFEAVSKSASAFNTEKLLWLNHHYINHLPADYVA
THLVLHIEQQGIDTRTGPQLAALVKLLGERCKTLKEIAASCRYFYEDFSEFDADAAKKHLRPVAVLALEAVRANLAALSE
WTPATVHAAIEQTAEALQVGMGKVGMPLRVAVTGTGQSPALDVTVHAIGQSRCLARIVKALDFIACRTQESAG

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=495, Percent_Identity=30.7070707070707, Blast_Score=219, Evalue=5e-57,
Organism=Escherichia coli, GI1788743, Length=471, Percent_Identity=82.8025477707006, Blast_Score=822, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=241, Percent_Identity=36.5145228215768, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1786895, Length=122, Percent_Identity=27.0491803278689, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=474, Percent_Identity=30.5907172995781, Blast_Score=214, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6324540, Length=492, Percent_Identity=31.0975609756098, Blast_Score=239, Evalue=8e-64,
Organism=Saccharomyces cerevisiae, GI6321192, Length=230, Percent_Identity=25.2173913043478, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324742, Length=218, Percent_Identity=25.2293577981651, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24665321, Length=492, Percent_Identity=31.0975609756098, Blast_Score=243, Evalue=1e-64,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_SODGM (Q2NSC4)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455356.1
- ProteinModelPortal:   Q2NSC4
- SMR:   Q2NSC4
- STRING:   Q2NSC4
- GeneID:   3868540
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG1676
- NMPDR:   fig|343509.6.peg.3558
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- PhylomeDB:   Q2NSC4
- ProtClustDB:   PRK01406
- BioCyc:   SGLO343509:SG1676-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52970; Mature: 52970

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 240-240

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKTRFAPSPTGYLHVGGARTALYSWLFARHLGGEFLLRIEDTDLERSTQPAIDAIMDG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHCC
MNWLNLDWDEGPYFQTKRFDRYNAVIDDMLSKGTAYHCYCSKERLETLREAQMARGEKPR
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCCC
YDGRCRDSHEHHADDEPCVVRFRNPQDGSVVFNDLIRGPIEFSNQELDDLIIRRIDGSPT
CCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCHHCCCCHHHHHHHHHCCCCCC
YNFCVVVDDFDMAITHVIRGEDHINNTPRQINILKALGAPVPAYAHVSMILGDDGKKLSK
EEEEEEEECHHHHHHHHHCCCHHCCCCCHHHHHHHHHCCCCCCHHEEEEEECCCCHHHHH
RHGAVGVMQYRDDGFLPEALLNYLVRLGWSHGDQEIFSVDEMKQLFSFEAVSKSASAFNT
HCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
EKLLWLNHHYINHLPADYVATHLVLHIEQQGIDTRTGPQLAALVKLLGERCKTLKEIAAS
CEEEEEECHHHHCCCHHHHHHHHHEEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
CRYFYEDFSEFDADAAKKHLRPVAVLALEAVRANLAALSEWTPATVHAAIEQTAEALQVG
HHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
MGKVGMPLRVAVTGTGQSPALDVTVHAIGQSRCLARIVKALDFIACRTQESAG
CCCCCCCEEEEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKIKTRFAPSPTGYLHVGGARTALYSWLFARHLGGEFLLRIEDTDLERSTQPAIDAIMDG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHCC
MNWLNLDWDEGPYFQTKRFDRYNAVIDDMLSKGTAYHCYCSKERLETLREAQMARGEKPR
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCCC
YDGRCRDSHEHHADDEPCVVRFRNPQDGSVVFNDLIRGPIEFSNQELDDLIIRRIDGSPT
CCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCHHCCCCHHHHHHHHHCCCCCC
YNFCVVVDDFDMAITHVIRGEDHINNTPRQINILKALGAPVPAYAHVSMILGDDGKKLSK
EEEEEEEECHHHHHHHHHCCCHHCCCCCHHHHHHHHHCCCCCCHHEEEEEECCCCHHHHH
RHGAVGVMQYRDDGFLPEALLNYLVRLGWSHGDQEIFSVDEMKQLFSFEAVSKSASAFNT
HCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
EKLLWLNHHYINHLPADYVATHLVLHIEQQGIDTRTGPQLAALVKLLGERCKTLKEIAAS
CEEEEEECHHHHCCCHHHHHHHHHEEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
CRYFYEDFSEFDADAAKKHLRPVAVLALEAVRANLAALSEWTPATVHAAIEQTAEALQVG
HHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
MGKVGMPLRVAVTGTGQSPALDVTVHAIGQSRCLARIVKALDFIACRTQESAG
CCCCCCCEEEEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA