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The map label for this gene is truA

Identifier: 85059597

GI number: 85059597

Start: 2774911

End: 2775726

Strand: Reverse

Name: truA

Synonym: SG1619

Alternate gene names: 85059597

Gene position: 2775726-2774911 (Counterclockwise)

Preceding gene: 85059598

Following gene: 85059596

Centisome position: 66.55

GC content: 61.64

Gene sequence:

>816_bases
TTGCCGGCGGTGGCCAATGACGGCACGATGAAGCTGGCGCTCGGGATTGAATATGACGGCAGCGCCTATTACGGCTGGCA
GCGTCAGCAGGAAGTGGCGAGCGTGCAAGCGTGCCTCGAACGCGCGCTGTCGACGGTCGCCGATGAAACTGTCACGGTTT
ATTGCGCCGGCCGTACCGACGCCGGCGTGCATGCCACCGGGCAGGTCGTTCATTTCGACACCCGATCGCGGCGACCGGAT
ACCGCCTGGACGCTTGGCGTCAACGCCAATTTGCCGGCCGATATTGCCGTGCGCTGGGTAGCGCCGGTGACGGACGATTT
TCATGCGCGTTTCAGCGCGACCGCCCGGCGCTACCGCTATATTATTTATAATCATCCCCTGCGCCCGGCACTGCTTGGCC
GCGGGTTAACCCATTATTATCATCCGCTCGATGCGCCGTCGATGGCGCGCGCCGGCCAGTGTTTATTGGGCGAAAACGAT
TTCACGTCTTTTCGCGCTCTGCAGTGCCAGTCGCGCAGCCCCTGGCGCAACGTGCACCATCTGTACGTCACGCGACAGGG
ACGCTATGTCGTGGTTGATATCAAAGCCAATGCTTTTGTTCATCATATGGTTCGTAATATTGTAGGCAGTCTGCTAGAAG
TGGGGTGCGGCAACCGGCCGGAAAGCTGGATCGCCGACCTGCTGGCGTGCCGCGACCGGACACAGGCCGGCGCTACCGCC
CGCGCGGAGGGGCTTTACCTGGTGGCGGTGGATTATCCGTCGCGTTTTTCCCTGCCCGCGTCGTCGTCGCCGGGGCCGTT
ATTTCTTGCGGATTAA

Upstream 100 bases:

>100_bases
GCGGCGCCCTGATGGCGGTGTTGACCGCGGAGAAACTGATGACCGAGGCCAGCGCGTGGTGACACTACAAACGGTGCCCC
TGCCCGGCGCGGACGAGGCA

Downstream 100 bases:

>100_bases
CCGCTTACTTTTTATCGACTTGCGTTACCGGGAAAACTGAATGGAATATATTAAATTTATTATCGATTTTATTTTGCACA
TTGATGTACATCTGGCAGAG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MPAVANDGTMKLALGIEYDGSAYYGWQRQQEVASVQACLERALSTVADETVTVYCAGRTDAGVHATGQVVHFDTRSRRPD
TAWTLGVNANLPADIAVRWVAPVTDDFHARFSATARRYRYIIYNHPLRPALLGRGLTHYYHPLDAPSMARAGQCLLGEND
FTSFRALQCQSRSPWRNVHHLYVTRQGRYVVVDIKANAFVHHMVRNIVGSLLEVGCGNRPESWIADLLACRDRTQAGATA
RAEGLYLVAVDYPSRFSLPASSSPGPLFLAD

Sequences:

>Translated_271_residues
MPAVANDGTMKLALGIEYDGSAYYGWQRQQEVASVQACLERALSTVADETVTVYCAGRTDAGVHATGQVVHFDTRSRRPD
TAWTLGVNANLPADIAVRWVAPVTDDFHARFSATARRYRYIIYNHPLRPALLGRGLTHYYHPLDAPSMARAGQCLLGEND
FTSFRALQCQSRSPWRNVHHLYVTRQGRYVVVDIKANAFVHHMVRNIVGSLLEVGCGNRPESWIADLLACRDRTQAGATA
RAEGLYLVAVDYPSRFSLPASSSPGPLFLAD
>Mature_270_residues
PAVANDGTMKLALGIEYDGSAYYGWQRQQEVASVQACLERALSTVADETVTVYCAGRTDAGVHATGQVVHFDTRSRRPDT
AWTLGVNANLPADIAVRWVAPVTDDFHARFSATARRYRYIIYNHPLRPALLGRGLTHYYHPLDAPSMARAGQCLLGENDF
TSFRALQCQSRSPWRNVHHLYVTRQGRYVVVDIKANAFVHHMVRNIVGSLLEVGCGNRPESWIADLLACRDRTQAGATAR
AEGLYLVAVDYPSRFSLPASSSPGPLFLAD

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=268, Percent_Identity=30.5970149253731, Blast_Score=75, Evalue=5e-14,
Organism=Escherichia coli, GI1788657, Length=261, Percent_Identity=77.3946360153257, Blast_Score=420, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 29936; Mature: 29805

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAVANDGTMKLALGIEYDGSAYYGWQRQQEVASVQACLERALSTVADETVTVYCAGRTD
CCCCCCCCCEEEEEEEEECCCEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
AGVHATGQVVHFDTRSRRPDTAWTLGVNANLPADIAVRWVAPVTDDFHARFSATARRYRY
CCCEECCEEEEECCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHEEE
IIYNHPLRPALLGRGLTHYYHPLDAPSMARAGQCLLGENDFTSFRALQCQSRSPWRNVHH
EEEECCCCHHHHHCCHHHHCCCCCCCCHHHCCCEEECCCCCCCEEEEEECCCCCCCCCEE
LYVTRQGRYVVVDIKANAFVHHMVRNIVGSLLEVGCGNRPESWIADLLACRDRTQAGATA
EEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCE
RAEGLYLVAVDYPSRFSLPASSSPGPLFLAD
ECCCEEEEEECCCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure 
PAVANDGTMKLALGIEYDGSAYYGWQRQQEVASVQACLERALSTVADETVTVYCAGRTD
CCCCCCCCEEEEEEEEECCCEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
AGVHATGQVVHFDTRSRRPDTAWTLGVNANLPADIAVRWVAPVTDDFHARFSATARRYRY
CCCEECCEEEEECCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHEEE
IIYNHPLRPALLGRGLTHYYHPLDAPSMARAGQCLLGENDFTSFRALQCQSRSPWRNVHH
EEEECCCCHHHHHCCHHHHCCCCCCCCHHHCCCEEECCCCCCCEEEEEECCCCCCCCCEE
LYVTRQGRYVVVDIKANAFVHHMVRNIVGSLLEVGCGNRPESWIADLLACRDRTQAGATA
EEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCE
RAEGLYLVAVDYPSRFSLPASSSPGPLFLAD
ECCCEEEEEECCCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA