Click here to switch to the map view.

The map label for this gene is folC [H]

Identifier: 85059594

GI number: 85059594

Start: 2771742

End: 2773007

Strand: Reverse

Name: folC [H]

Synonym: SG1616

Alternate gene names: 85059594

Gene position: 2773007-2771742 (Counterclockwise)

Preceding gene: 85059595

Following gene: 85059593

Centisome position: 66.48

GC content: 64.45

Gene sequence:

>1266_bases
ATGGAAAATCTGCCTATCCCCCAAGCCACGTCGCCACTGGCCGCGTGGCTTTATTATCTTGAACGTCTGCACGCCAAACC
CATCGATTTGGGCCTGGAGCGGGTATGCCATGTGGCGACCGGCCTGAATCTGCTGTGCCCGGCCACCTGGGTCATTACCG
TTGGCGGCACCAACGGCAAGGGTACCACCTGCCGGCTGCTGGAAGCCATTTTGCTGGCAAGCGGGATGCGTGTCGGCGTC
TATAGCTCCCCGCATTTGCTGCGCTATACCGAACGGGTGCGCATTCAGGGTGGCGAATTGTCGGAAGCCGCCCATAGTGA
AGCGATGGCGGTTATCGAAGCCGGCCGTGGCGCGACGTCGTTGAGCTATTTCGAGTTCGGTACGCTGGCAGCGCTGCAGC
TTTTTCGGCAAGCGGCGCTGGATGTGGTGATCCTTGAGGTCGGGCTGGGGGGGCGGTTGGACGCGACCAATATCGTCGAT
GCCGACGTCGCCGTAACGACCTCCATCGCCCTCGATCATACCGATTGGCTGGGACTGGACCGTGCTAGTATCGCGCGGGA
AAAGGCCGGTATCTTCCGTCGCGGCAAACCGGCGGTCGTGGGTGAGCCCAACCGGCCGGCGACGCTCGACGCTGCCGACT
GCGGCGCAGCGCTATACGCCCGCGATCGCGATTGGTGGTGGCAGGACGACGGCCAGCACTGGCGCTGGTGGGACAATGTG
CACGAGCTTACCGCACTGCCGCTGCCGGCCATTCCGCTGGAAAACGCGGCCACGGCGCTGGCCGCCGTTGCCCGCCTGCC
GTTCGCGGTACCGGAAGCGGCGGTGCGTCAGGGGCTGCGCGAGGCTTCGCTCCCGGGACGTTTTCAATGTATCGGCCGGG
CGCCGCTGATCATTCTGGACGTAGCGCACAATCCCCATGCCGCCGGCTATCTGGCCCGTCGTCTGGCGGCGTTGCCCCGC
ACCGGCAAAGTCCGGGCGGTGGTCGGGATGCTGGCGGATAAAGATATTCCCGGCACGCTTGCCTGCCTGCGCGGGCAGGT
AGATGTTTGGTATTGCGCATCGCTTGACGGGCCGCGTGCTGCCAACGCCGCGCAACTGGCGGCGCATCTTGACAGCGACG
CGCAACGGTTTGAGGATGTGCCAAGCGCCTGGCATCAGGCAATAGCGGACGCCGCGCCCGAGGATTGCGTGCTGGTATTC
GGCTCTTTCCATACCGTGGCACCGGTCCTGGCGCTGGCTGAAAGGGAGACGTATTGTGGCAAGTAA

Upstream 100 bases:

>100_bases
GATCATCAGGACGAGGTATAAAGGGCGGCGTAAGCCTGCCCGCAGCCATCAGCCGGGGGCACGCTTAGTGAAAACCCAGC
ATAAGCCAATGAACGGACGT

Downstream 100 bases:

>100_bases
ATTTCAAAACCGCCTGGTCGGGACCATTATCCTGGTCGCCCTCGGCGTCATCATTTTGCCCGGTCTGCTGGACGGTAAAA
AGAAGCACTATCAGGACGAG

Product: bifunctional folylpolyglutamate synthase/ dihydrofolate synthase

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MENLPIPQATSPLAAWLYYLERLHAKPIDLGLERVCHVATGLNLLCPATWVITVGGTNGKGTTCRLLEAILLASGMRVGV
YSSPHLLRYTERVRIQGGELSEAAHSEAMAVIEAGRGATSLSYFEFGTLAALQLFRQAALDVVILEVGLGGRLDATNIVD
ADVAVTTSIALDHTDWLGLDRASIAREKAGIFRRGKPAVVGEPNRPATLDAADCGAALYARDRDWWWQDDGQHWRWWDNV
HELTALPLPAIPLENAATALAAVARLPFAVPEAAVRQGLREASLPGRFQCIGRAPLIILDVAHNPHAAGYLARRLAALPR
TGKVRAVVGMLADKDIPGTLACLRGQVDVWYCASLDGPRAANAAQLAAHLDSDAQRFEDVPSAWHQAIADAAPEDCVLVF
GSFHTVAPVLALAERETYCGK

Sequences:

>Translated_421_residues
MENLPIPQATSPLAAWLYYLERLHAKPIDLGLERVCHVATGLNLLCPATWVITVGGTNGKGTTCRLLEAILLASGMRVGV
YSSPHLLRYTERVRIQGGELSEAAHSEAMAVIEAGRGATSLSYFEFGTLAALQLFRQAALDVVILEVGLGGRLDATNIVD
ADVAVTTSIALDHTDWLGLDRASIAREKAGIFRRGKPAVVGEPNRPATLDAADCGAALYARDRDWWWQDDGQHWRWWDNV
HELTALPLPAIPLENAATALAAVARLPFAVPEAAVRQGLREASLPGRFQCIGRAPLIILDVAHNPHAAGYLARRLAALPR
TGKVRAVVGMLADKDIPGTLACLRGQVDVWYCASLDGPRAANAAQLAAHLDSDAQRFEDVPSAWHQAIADAAPEDCVLVF
GSFHTVAPVLALAERETYCGK
>Mature_421_residues
MENLPIPQATSPLAAWLYYLERLHAKPIDLGLERVCHVATGLNLLCPATWVITVGGTNGKGTTCRLLEAILLASGMRVGV
YSSPHLLRYTERVRIQGGELSEAAHSEAMAVIEAGRGATSLSYFEFGTLAALQLFRQAALDVVILEVGLGGRLDATNIVD
ADVAVTTSIALDHTDWLGLDRASIAREKAGIFRRGKPAVVGEPNRPATLDAADCGAALYARDRDWWWQDDGQHWRWWDNV
HELTALPLPAIPLENAATALAAVARLPFAVPEAAVRQGLREASLPGRFQCIGRAPLIILDVAHNPHAAGYLARRLAALPR
TGKVRAVVGMLADKDIPGTLACLRGQVDVWYCASLDGPRAANAAQLAAHLDSDAQRFEDVPSAWHQAIADAAPEDCVLVF
GSFHTVAPVLALAERETYCGK

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=297, Percent_Identity=32.6599326599327, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI66932984, Length=297, Percent_Identity=32.6599326599327, Blast_Score=118, Evalue=9e-27,
Organism=Escherichia coli, GI1788654, Length=417, Percent_Identity=61.1510791366906, Blast_Score=474, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI71984923, Length=434, Percent_Identity=27.8801843317972, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17553148, Length=434, Percent_Identity=27.6497695852535, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17553150, Length=434, Percent_Identity=27.8801843317972, Blast_Score=128, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6323760, Length=313, Percent_Identity=28.1150159744409, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6324815, Length=194, Percent_Identity=32.4742268041237, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24641571, Length=151, Percent_Identity=40.3973509933775, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24581568, Length=146, Percent_Identity=38.3561643835616, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 45253; Mature: 45253

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLPIPQATSPLAAWLYYLERLHAKPIDLGLERVCHVATGLNLLCPATWVITVGGTNGK
CCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEECCEEEEEEECCCCCC
GTTCRLLEAILLASGMRVGVYSSPHLLRYTERVRIQGGELSEAAHSEAMAVIEAGRGATS
CHHHHHHHHHHHHCCCEEEECCCCHHHEEEHHHEECCCCHHHHHHHHHHHHHCCCCCCCE
LSYFEFGTLAALQLFRQAALDVVILEVGLGGRLDATNIVDADVAVTTSIALDHTDWLGLD
EHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCEECCCCEEEEEEEECCCCCCCCC
RASIAREKAGIFRRGKPAVVGEPNRPATLDAADCGAALYARDRDWWWQDDGQHWRWWDNV
HHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHCCCEEEECCCCCCCCCCCCCCCHHHHH
HELTALPLPAIPLENAATALAAVARLPFAVPEAAVRQGLREASLPGRFQCIGRAPLIILD
HHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCEEEEE
VAHNPHAAGYLARRLAALPRTGKVRAVVGMLADKDIPGTLACLRGQVDVWYCASLDGPRA
ECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCEEEEEECCCCCCC
ANAAQLAAHLDSDAQRFEDVPSAWHQAIADAAPEDCVLVFGSFHTVAPVLALAERETYCG
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCC
K
C
>Mature Secondary Structure
MENLPIPQATSPLAAWLYYLERLHAKPIDLGLERVCHVATGLNLLCPATWVITVGGTNGK
CCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEECCEEEEEEECCCCCC
GTTCRLLEAILLASGMRVGVYSSPHLLRYTERVRIQGGELSEAAHSEAMAVIEAGRGATS
CHHHHHHHHHHHHCCCEEEECCCCHHHEEEHHHEECCCCHHHHHHHHHHHHHCCCCCCCE
LSYFEFGTLAALQLFRQAALDVVILEVGLGGRLDATNIVDADVAVTTSIALDHTDWLGLD
EHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCEECCCCEEEEEEEECCCCCCCCC
RASIAREKAGIFRRGKPAVVGEPNRPATLDAADCGAALYARDRDWWWQDDGQHWRWWDNV
HHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHCCCEEEECCCCCCCCCCCCCCCHHHHH
HELTALPLPAIPLENAATALAAVARLPFAVPEAAVRQGLREASLPGRFQCIGRAPLIILD
HHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCEEEEE
VAHNPHAAGYLARRLAALPRTGKVRAVVGMLADKDIPGTLACLRGQVDVWYCASLDGPRA
ECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCEEEEEECCCCCCC
ANAAQLAAHLDSDAQRFEDVPSAWHQAIADAAPEDCVLVFGSFHTVAPVLALAERETYCG
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]