Click here to switch to the map view.

The map label for this gene is nuoF [H]

Identifier: 85059575

GI number: 85059575

Start: 2743723

End: 2745078

Strand: Reverse

Name: nuoF [H]

Synonym: SG1597

Alternate gene names: 85059575

Gene position: 2745078-2743723 (Counterclockwise)

Preceding gene: 85059576

Following gene: 85059574

Centisome position: 65.81

GC content: 61.06

Gene sequence:

>1356_bases
ATGACCAAAACCATTGTCCGCTTGGCGGAAACGCACCCGCTGACCTGGCGGCTGCGCGATGACCATCAGCCGGTGTGGCT
GGACGAATATCAGAGCAAAAACGGCTATGTCGGCGCACGCAAGGCGTTGAGCGGCATGGCGCAGGACGATATCGTTACGC
TTGTGAAAGACTCGGGCCTGAAAGGACGCGGCGGCGCCGGCTTTTCGACCGGCCTGAAGTGGAGCCTGATGCCGAAAGAT
CCCGCCATGAACATTCGCTATTTGCTGTGCAATGCTGATGAGATGGAGCCGGGCACCTATAAAGACCGTTTGCTGATGGA
GCAACTGCCGCACCTGCTGGTGGAAGGGATGCTGATCGCCGCCTTCGCTCTGAAAGCCTATCGCGGCTATATTTTCCTGC
GCGGCGAGTATATCGAGGCGGCGGCGAACCTGCGCCGCGCGATAGCCGAGGCCACCGAGGCCGGTTGGTTGGGCAAAAAT
ATTCTGGGCAGCGGGTTTGACTTCGAGCTTATCGTCCACACCGGCGCCGGGCGCTATATCTGCGGCGAAGAAACCGCGCT
GATCAATTCCCTGGAGGGGCGCCGCGCTAACCCGCGCTCGAAACCGCCGTTTCCCGCCAGCGCTGGCGCCTGGGGTAAAC
CCACCTGTGTCAACAACGTCGAAACATTATGCAATGTGCCGGCCATCCTGGAGCACGGGGTAGACTGGTATCAAGGCATC
ACCGCCGGCAAGAGCAACAGCAACGACGCCGGCACCAAGCTGATGGGCTTCTCCGGTCGGGTGAAGAACCCCGGCGTCTG
GGAACTGCCGTTCGGCATCACCGCGCGCGAGATCCTCGAGGATTACGCCGGCGGCATGCGCGATGGCCTGACATTGAAAG
CCTGGCAGCCCGGCGGCGCCGGCACCGACTTTTTGATCCAGGATCACCTGGATCTGCCGATGGATTTTGAGAATATTGGC
AAAGCGGGCAGCCGGCTCGGCACCGCTCTGGCGATGGCGGTGGACAACGAAATTAACATGGTGTCGTTAACGCGCAATCT
GGAAGAGTTTTTTGCCCGGGAATCCTGCGGCTGGTGTACGCCGTGCCGCGACGGCCTGCCGTGGAGCGTCAAGCTGCTGC
GTGCGCTGGAGAGCGGCAAAGGCCAGCCCGGGGATATTGAAACCCTGGAGCAGCTGTGCCGCTTTCTTGGCCCCGGCAAA
ACCTTCTGCGCCCATGCACCCGGGGCGGTGGAGCCGTTGCAAAGCGCGATTAAATATTTCCGCTCCGAGTTCGAGGCGGG
TATCAGCGTTGAACATTTGGGCAATACGCGCGCCATCGCCGGCGTGCAGCCCAACCTATTAAAAGCGCGCTGGTAA

Upstream 100 bases:

>100_bases
GTCTGGGCAATTGCGACAAGGGACCGACCATGATGGTGGATGACGATACCCACTTGCATCTGACACCGGAAGGTATTGGT
CCGCTGCTGGAGCAATATCG

Downstream 100 bases:

>100_bases
TCGTCCATGCGACATGAGCGGGTCAGGCGGGCGCACGCGCTTTGCACCCTGAACGCATTTTTGATTAACGCCCGCCGTTG
GCGGGCCTCACCTGAGCATG

Product: NADH dehydrogenase I subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F; NDH-1 subunit F; NUO6 [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MTKTIVRLAETHPLTWRLRDDHQPVWLDEYQSKNGYVGARKALSGMAQDDIVTLVKDSGLKGRGGAGFSTGLKWSLMPKD
PAMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLIAAFALKAYRGYIFLRGEYIEAAANLRRAIAEATEAGWLGKN
ILGSGFDFELIVHTGAGRYICGEETALINSLEGRRANPRSKPPFPASAGAWGKPTCVNNVETLCNVPAILEHGVDWYQGI
TAGKSNSNDAGTKLMGFSGRVKNPGVWELPFGITAREILEDYAGGMRDGLTLKAWQPGGAGTDFLIQDHLDLPMDFENIG
KAGSRLGTALAMAVDNEINMVSLTRNLEEFFARESCGWCTPCRDGLPWSVKLLRALESGKGQPGDIETLEQLCRFLGPGK
TFCAHAPGAVEPLQSAIKYFRSEFEAGISVEHLGNTRAIAGVQPNLLKARW

Sequences:

>Translated_451_residues
MTKTIVRLAETHPLTWRLRDDHQPVWLDEYQSKNGYVGARKALSGMAQDDIVTLVKDSGLKGRGGAGFSTGLKWSLMPKD
PAMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLIAAFALKAYRGYIFLRGEYIEAAANLRRAIAEATEAGWLGKN
ILGSGFDFELIVHTGAGRYICGEETALINSLEGRRANPRSKPPFPASAGAWGKPTCVNNVETLCNVPAILEHGVDWYQGI
TAGKSNSNDAGTKLMGFSGRVKNPGVWELPFGITAREILEDYAGGMRDGLTLKAWQPGGAGTDFLIQDHLDLPMDFENIG
KAGSRLGTALAMAVDNEINMVSLTRNLEEFFARESCGWCTPCRDGLPWSVKLLRALESGKGQPGDIETLEQLCRFLGPGK
TFCAHAPGAVEPLQSAIKYFRSEFEAGISVEHLGNTRAIAGVQPNLLKARW
>Mature_450_residues
TKTIVRLAETHPLTWRLRDDHQPVWLDEYQSKNGYVGARKALSGMAQDDIVTLVKDSGLKGRGGAGFSTGLKWSLMPKDP
AMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLIAAFALKAYRGYIFLRGEYIEAAANLRRAIAEATEAGWLGKNI
LGSGFDFELIVHTGAGRYICGEETALINSLEGRRANPRSKPPFPASAGAWGKPTCVNNVETLCNVPAILEHGVDWYQGIT
AGKSNSNDAGTKLMGFSGRVKNPGVWELPFGITAREILEDYAGGMRDGLTLKAWQPGGAGTDFLIQDHLDLPMDFENIGK
AGSRLGTALAMAVDNEINMVSLTRNLEEFFARESCGWCTPCRDGLPWSVKLLRALESGKGQPGDIETLEQLCRFLGPGKT
FCAHAPGAVEPLQSAIKYFRSEFEAGISVEHLGNTRAIAGVQPNLLKARW

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=379, Percent_Identity=44.3271767810026, Blast_Score=309, Evalue=4e-84,
Organism=Homo sapiens, GI260656005, Length=379, Percent_Identity=44.3271767810026, Blast_Score=308, Evalue=6e-84,
Organism=Escherichia coli, GI1788620, Length=449, Percent_Identity=86.4142538975501, Blast_Score=804, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17531727, Length=386, Percent_Identity=42.7461139896373, Blast_Score=302, Evalue=2e-82,
Organism=Drosophila melanogaster, GI19920794, Length=379, Percent_Identity=44.3271767810026, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI45550452, Length=380, Percent_Identity=44.4736842105263, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24653819, Length=378, Percent_Identity=34.3915343915344, Blast_Score=228, Evalue=7e-60,
Organism=Drosophila melanogaster, GI24653817, Length=356, Percent_Identity=32.3033707865169, Blast_Score=201, Evalue=7e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 49290; Mature: 49159

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTIVRLAETHPLTWRLRDDHQPVWLDEYQSKNGYVGARKALSGMAQDDIVTLVKDSGL
CCHHHHHHHHCCCEEEEECCCCCCEEHHHHCCCCCCCHHHHHHCCCCHHHEEEEECCCCC
KGRGGAGFSTGLKWSLMPKDPAMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLIA
CCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AFALKAYRGYIFLRGEYIEAAANLRRAIAEATEAGWLGKNILGSGFDFELIVHTGAGRYI
HHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEECCCCCEE
CGEETALINSLEGRRANPRSKPPFPASAGAWGKPTCVNNVETLCNVPAILEHGVDWYQGI
ECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHCCCHHHHCC
TAGKSNSNDAGTKLMGFSGRVKNPGVWELPFGITAREILEDYAGGMRDGLTLKAWQPGGA
CCCCCCCCCCCCEEECCCCCCCCCCEEECCCCCCHHHHHHHHCCCCCCCCEEEEECCCCC
GTDFLIQDHLDLPMDFENIGKAGSRLGTALAMAVDNEINMVSLTRNLEEFFARESCGWCT
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCEEHHHHHHHHHHHHCCCCCCC
PCRDGLPWSVKLLRALESGKGQPGDIETLEQLCRFLGPGKTFCAHAPGAVEPLQSAIKYF
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHH
RSEFEAGISVEHLGNTRAIAGVQPNLLKARW
HHHHHCCCEEECCCCCEEECCCCCCHHCCCC
>Mature Secondary Structure 
TKTIVRLAETHPLTWRLRDDHQPVWLDEYQSKNGYVGARKALSGMAQDDIVTLVKDSGL
CHHHHHHHHCCCEEEEECCCCCCEEHHHHCCCCCCCHHHHHHCCCCHHHEEEEECCCCC
KGRGGAGFSTGLKWSLMPKDPAMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLIA
CCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AFALKAYRGYIFLRGEYIEAAANLRRAIAEATEAGWLGKNILGSGFDFELIVHTGAGRYI
HHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEECCCCCEE
CGEETALINSLEGRRANPRSKPPFPASAGAWGKPTCVNNVETLCNVPAILEHGVDWYQGI
ECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHCCCHHHHCC
TAGKSNSNDAGTKLMGFSGRVKNPGVWELPFGITAREILEDYAGGMRDGLTLKAWQPGGA
CCCCCCCCCCCCEEECCCCCCCCCCEEECCCCCCHHHHHHHHCCCCCCCCEEEEECCCCC
GTDFLIQDHLDLPMDFENIGKAGSRLGTALAMAVDNEINMVSLTRNLEEFFARESCGWCT
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCEEHHHHHHHHHHHHCCCCCCC
PCRDGLPWSVKLLRALESGKGQPGDIETLEQLCRFLGPGKTFCAHAPGAVEPLQSAIKYF
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHH
RSEFEAGISVEHLGNTRAIAGVQPNLLKARW
HHHHHCCCEEECCCCCEEECCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7690854; 8157582; 9205837; 9278503; 8366049 [H]