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The map label for this gene is pntA

Identifier: 85059466

GI number: 85059466

Start: 2483672

End: 2485204

Strand: Reverse

Name: pntA

Synonym: SG1488

Alternate gene names: 85059466

Gene position: 2485204-2483672 (Counterclockwise)

Preceding gene: 85059468

Following gene: 85059465

Centisome position: 59.58

GC content: 56.43

Gene sequence:

>1533_bases
ATGCGTATTGGTGTACCTAAAGAACGCGTTGCCAACGAAGCCCGGGTTGCCGCCACGCCTAAAACCGTCGAGCAATTGCT
TAAGTTGGGTTTTAGTATTGCGATGGAAAGCGGCGCCGGCACGCTGGCGAGTTTCGAGGATGCCGCTTTTGTCGCGGCGG
GCGCGACGCTGACAACTACCTCTGAAGTTTGGCAGTCGGATATCATTCTCAAAGTCAACGCGCCGACTGAGGATGAAATT
GCCTTGCTGCAAAGCGGCACGACATTGATAAGTTTTATTTGGCCGGCGCAAAATGCCGACCTGCTAACGGCGCTGCGCGC
TAAAAACGTGACCGTCATGGCGATGGACGCTGTGCCGCGTATCTCCCGCGCTCAGTCGCTGGACGCCCTGAGCTCGATGG
CCAATATCGCCGGTTATCGCGCCATTGTTGAAGCGGCTCACGAATTCGGCCGCTTCTTTACCGGCCAGATTACGGCGGCG
GGCAAAGTGCCGCCGGCCAAAGTTATGGTGATAGGCGCCGGGGTCGCGGGGCTGGCCGCCATCGGTACCGCGGGCAGCCT
GGGCGCCGTGGTTCGCGCCTTCGACACCCGTCCCGAGGTGAAAGAGCAGGTGCAAAGTATGGGCGCCGAGTTCCTTGAGC
TGGATTTCAAGGAGGAGGCGGGCGGTAGCGATGGTTACGCCAAGGTGATGTCGGACGCTTTTATCAAAGCCGAAATGGCG
CTGTTCGCCGCACAGGCGCAAGAAGTCGATATTATCGTCACCACCGCGCTGATTCCGGGCAAACAGGCCCCCCGCTTGAT
TACCAAAGAGATGGTGACCAGCATGAAGCCGGGAAGCGTTATTGTTGATCTGGCGGCGCAAACCGGCGGTAACTGTGAGC
TCACCGTGGCGGACACCGTAACGGTGACCGATAATGGCGTTCGTATTATCGGCTATACCGATTTACCCAGCCGGTTACCT
ACCCAATCCTCCCAACTGTACGCCACCAACCTCATCAATCTGCTGAAGTTACTTTGTAAAGAGAAACAGGGCGATATCGT
GGTGGATTTCGACGACATGGTTATTCGCGGCGTCACCGTGATACGCGATGGCGAATTGACCTGGCCCGCGCCGCCTATCA
CTGTTTCCGCCCAGCCGCAGCCGGCGCATGCGGCCCCTGCCGCAAAACCCGCAGAACCGCCCCGTCCGGCGTCGCCGCTG
CGTAAATATGCCCTTATCGCTTTGGCGATTATTTTATTTTGCTGGCTGGCGAATGCGGCTCCGCGCGATTTTCTCTCGCA
TTTTACGGTGTTCGCCCTGGCCTGTGTCGTAGGCTATTACGTTGTCTGGAATGTCAGTCATGCATTGCATACCCCGTTAA
TGTCGGTCACCAATGCTATTTCCGGCATTATTGTCGTTGGCGCATTATTGCAGATAGGTGATGGCGGATGGGTTTCATTC
CTGTCGTTTATCGCCGTGCTGATCGCCAGCATCAATATCTTTGGTGGTTTCACCGTCACTCAGCGCATGCTGAAAATGTT
TCGTAAAAATTAA

Upstream 100 bases:

>100_bases
TTGACGCCTGCTGGATTTACCGGGCGGCGGCCCGTAATGCCGCGCGTTCCGGTCCCGGCAACCTGTTATTTACAAAAGAA
CTGAATAATAAGGGAAAGCT

Downstream 100 bases:

>100_bases
GGGGTGAACCATGTCAGAAGGACTCGTCACGGCGGCTTATATCGCCGCCGCCATTTTATTTATTTTTAGCCTGGCGGGGC
TATCCAAGCACGAAACATCG

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MRIGVPKERVANEARVAATPKTVEQLLKLGFSIAMESGAGTLASFEDAAFVAAGATLTTTSEVWQSDIILKVNAPTEDEI
ALLQSGTTLISFIWPAQNADLLTALRAKNVTVMAMDAVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
GKVPPAKVMVIGAGVAGLAAIGTAGSLGAVVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGGSDGYAKVMSDAFIKAEMA
LFAAQAQEVDIIVTTALIPGKQAPRLITKEMVTSMKPGSVIVDLAAQTGGNCELTVADTVTVTDNGVRIIGYTDLPSRLP
TQSSQLYATNLINLLKLLCKEKQGDIVVDFDDMVIRGVTVIRDGELTWPAPPITVSAQPQPAHAAPAAKPAEPPRPASPL
RKYALIALAIILFCWLANAAPRDFLSHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGDGGWVSF
LSFIAVLIASINIFGGFTVTQRMLKMFRKN

Sequences:

>Translated_510_residues
MRIGVPKERVANEARVAATPKTVEQLLKLGFSIAMESGAGTLASFEDAAFVAAGATLTTTSEVWQSDIILKVNAPTEDEI
ALLQSGTTLISFIWPAQNADLLTALRAKNVTVMAMDAVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
GKVPPAKVMVIGAGVAGLAAIGTAGSLGAVVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGGSDGYAKVMSDAFIKAEMA
LFAAQAQEVDIIVTTALIPGKQAPRLITKEMVTSMKPGSVIVDLAAQTGGNCELTVADTVTVTDNGVRIIGYTDLPSRLP
TQSSQLYATNLINLLKLLCKEKQGDIVVDFDDMVIRGVTVIRDGELTWPAPPITVSAQPQPAHAAPAAKPAEPPRPASPL
RKYALIALAIILFCWLANAAPRDFLSHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGDGGWVSF
LSFIAVLIASINIFGGFTVTQRMLKMFRKN
>Mature_510_residues
MRIGVPKERVANEARVAATPKTVEQLLKLGFSIAMESGAGTLASFEDAAFVAAGATLTTTSEVWQSDIILKVNAPTEDEI
ALLQSGTTLISFIWPAQNADLLTALRAKNVTVMAMDAVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
GKVPPAKVMVIGAGVAGLAAIGTAGSLGAVVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGGSDGYAKVMSDAFIKAEMA
LFAAQAQEVDIIVTTALIPGKQAPRLITKEMVTSMKPGSVIVDLAAQTGGNCELTVADTVTVTDNGVRIIGYTDLPSRLP
TQSSQLYATNLINLLKLLCKEKQGDIVVDFDDMVIRGVTVIRDGELTWPAPPITVSAQPQPAHAAPAAKPAEPPRPASPL
RKYALIALAIILFCWLANAAPRDFLSHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGDGGWVSF
LSFIAVLIASINIFGGFTVTQRMLKMFRKN

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=532, Percent_Identity=50.3759398496241, Blast_Score=478, Evalue=1e-135,
Organism=Homo sapiens, GI122939153, Length=532, Percent_Identity=50.3759398496241, Blast_Score=478, Evalue=1e-135,
Organism=Escherichia coli, GI1787887, Length=510, Percent_Identity=82.156862745098, Blast_Score=842, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=521, Percent_Identity=52.5911708253359, Blast_Score=498, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 54043; Mature: 54043

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00836 ALADH_PNT_1 ; PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGVPKERVANEARVAATPKTVEQLLKLGFSIAMESGAGTLASFEDAAFVAAGATLTTT
CCCCCCHHHHCCCHHCCCCHHHHHHHHHCCHHEEECCCCCCCCCCCCCCEEECCCEEEEH
SEVWQSDIILKVNAPTEDEIALLQSGTTLISFIWPAQNADLLTALRAKNVTVMAMDAVPR
HHHHCCCEEEEECCCCCHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCEEEEEECCCCC
ISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCHHHHHH
IGTAGSLGAVVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGGSDGYAKVMSDAFIKAEMA
HCCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHEEECHHHCCCCCCHHHHHHHHHHHHHHH
LFAAQAQEVDIIVTTALIPGKQAPRLITKEMVTSMKPGSVIVDLAAQTGGNCELTVADTV
HHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEEE
TVTDNGVRIIGYTDLPSRLPTQSSQLYATNLINLLKLLCKEKQGDIVVDFDDMVIRGVTV
EEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHCEEE
IRDGELTWPAPPITVSAQPQPAHAAPAAKPAEPPRPASPLRKYALIALAIILFCWLANAA
EECCCEECCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
PRDFLSHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGDGGWVSF
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LSFIAVLIASINIFGGFTVTQRMLKMFRKN
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIGVPKERVANEARVAATPKTVEQLLKLGFSIAMESGAGTLASFEDAAFVAAGATLTTT
CCCCCCHHHHCCCHHCCCCHHHHHHHHHCCHHEEECCCCCCCCCCCCCCEEECCCEEEEH
SEVWQSDIILKVNAPTEDEIALLQSGTTLISFIWPAQNADLLTALRAKNVTVMAMDAVPR
HHHHCCCEEEEECCCCCHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCEEEEEECCCCC
ISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCHHHHHH
IGTAGSLGAVVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGGSDGYAKVMSDAFIKAEMA
HCCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHEEECHHHCCCCCCHHHHHHHHHHHHHHH
LFAAQAQEVDIIVTTALIPGKQAPRLITKEMVTSMKPGSVIVDLAAQTGGNCELTVADTV
HHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEEE
TVTDNGVRIIGYTDLPSRLPTQSSQLYATNLINLLKLLCKEKQGDIVVDFDDMVIRGVTV
EEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHCEEE
IRDGELTWPAPPITVSAQPQPAHAAPAAKPAEPPRPASPLRKYALIALAIILFCWLANAA
EECCCEECCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
PRDFLSHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGDGGWVSF
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LSFIAVLIASINIFGGFTVTQRMLKMFRKN
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]