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The map label for this gene is rnfE

Identifier: 85059428

GI number: 85059428

Start: 2424303

End: 2425001

Strand: Reverse

Name: rnfE

Synonym: SG1450

Alternate gene names: 85059428

Gene position: 2425001-2424303 (Counterclockwise)

Preceding gene: 85059429

Following gene: 85059427

Centisome position: 58.14

GC content: 58.94

Gene sequence:

>699_bases
ATGAATGAAGCGAAACGCATCCTCCTGGAAGGCCTGTGGCGCAATAACTCGTCACTGGTGCAATTACTGGGTCTTTGTCC
ACTGCTGGCGGTATCCAATACCGCCACCAATGCACTCGGGTTGGGGCTTGCCACGACGCTGGTGCTGGTTTGCACCAATA
CCGCGGTGTCGGCCCTGCGCCGCTGGATCACCGGCGAAATCCGCATTCCCATTTATGTCATGATTATCGCCTCGGTGGTG
TCGGCGGTACAGATGCTTATCAACGCCTATGCGTTTGGTCTTTACCAATCCCTCGGTATTTTCATCCCGCTTATCGTGAC
TAACTGCATCGTTATCGGCCGGGCGGAAGCCTATGCCTCGCAGCGACCGCCAGCGCTGGCGGCGCTGGATGGGTTGTCCA
TCGGACTCGGCTCGACGTGCACGCTGCTTCTGCTCGGCGCCCTGCGCGAAGTGCTGGGTAACGGCACCCTGTTCGACGGC
GCGGACCAACTACTGGGCGCATGGGCGCGGGTGCTGCGCATCGAGGTATTCCATACCGATACTCCCTTTTTGCTGGCCAT
GTTGCCGCCTGGCGCGTTTATCGGCCTGGGGTTTATGCTGGTGGGGAAATACCTTATCGATCAACGCATGAAGGCCCGGC
AGCCAAAGGCGGCCCGACCGGTAGTGCTGCAACAAGGACAGCCATTAGCCGATGAATAA

Upstream 100 bases:

>100_bases
ACGCCGCGGGCCGTGATGAATGCCGTCAAGCGCACCGCCGGCTATTTGAAAAAGAACCTTAACGCGCTGGAAAAAATGCC
GCATTGTGGAGATTCATCCC

Downstream 100 bases:

>100_bases
AAGCAAGCGTTATGACATTCTGTGTCGCCTGCGGGCGAATAATCCCCATCCCACCACCGAGCTGATGTACCGCTCGCCTT
TCGAGCTGTTGATTGCAGTA

Product: electron transport complex RsxE subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MNEAKRILLEGLWRNNSSLVQLLGLCPLLAVSNTATNALGLGLATTLVLVCTNTAVSALRRWITGEIRIPIYVMIIASVV
SAVQMLINAYAFGLYQSLGIFIPLIVTNCIVIGRAEAYASQRPPALAALDGLSIGLGSTCTLLLLGALREVLGNGTLFDG
ADQLLGAWARVLRIEVFHTDTPFLLAMLPPGAFIGLGFMLVGKYLIDQRMKARQPKAARPVVLQQGQPLADE

Sequences:

>Translated_232_residues
MNEAKRILLEGLWRNNSSLVQLLGLCPLLAVSNTATNALGLGLATTLVLVCTNTAVSALRRWITGEIRIPIYVMIIASVV
SAVQMLINAYAFGLYQSLGIFIPLIVTNCIVIGRAEAYASQRPPALAALDGLSIGLGSTCTLLLLGALREVLGNGTLFDG
ADQLLGAWARVLRIEVFHTDTPFLLAMLPPGAFIGLGFMLVGKYLIDQRMKARQPKAARPVVLQQGQPLADE
>Mature_232_residues
MNEAKRILLEGLWRNNSSLVQLLGLCPLLAVSNTATNALGLGLATTLVLVCTNTAVSALRRWITGEIRIPIYVMIIASVV
SAVQMLINAYAFGLYQSLGIFIPLIVTNCIVIGRAEAYASQRPPALAALDGLSIGLGSTCTLLLLGALREVLGNGTLFDG
ADQLLGAWARVLRIEVFHTDTPFLLAMLPPGAFIGLGFMLVGKYLIDQRMKARQPKAARPVVLQQGQPLADE

Specific function: May be part of a membrane complex involved in electron transport

COG id: COG4660

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfE

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family

Homologues:

Organism=Escherichia coli, GI1787919, Length=227, Percent_Identity=74.4493392070485, Blast_Score=327, Evalue=6e-91,
Organism=Escherichia coli, GI1787914, Length=142, Percent_Identity=36.6197183098592, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNFE_SODGM (Q2NT00)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455130.1
- STRING:   Q2NT00
- GeneID:   3867267
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG1450
- NMPDR:   fig|343509.6.peg.2987
- eggNOG:   COG4660
- HOGENOM:   HBG624915
- OMA:   ENPVTKM
- PhylomeDB:   Q2NT00
- ProtClustDB:   PRK12405
- BioCyc:   SGLO343509:SG1450-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00478
- InterPro:   IPR003667
- InterPro:   IPR010968
- PIRSF:   PIRSF006102
- TIGRFAMs:   TIGR01948

Pfam domain/function: PF02508 Rnf-Nqr

EC number: NA

Molecular weight: Translated: 24825; Mature: 24825

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xdec39c4)-; HASH(0xf8f9840)-; HASH(0xf8cd590)-; HASH(0xf8eaa50)-; HASH(0xedf5bd0)-; HASH(0x94ae01c)-;

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEAKRILLEGLWRNNSSLVQLLGLCPLLAVSNTATNALGLGLATTLVLVCTNTAVSALR
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RWITGEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFIPLIVTNCIVIGRAEAYAS
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHC
QRPPALAALDGLSIGLGSTCTLLLLGALREVLGNGTLFDGADQLLGAWARVLRIEVFHTD
CCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHEEECC
TPFLLAMLPPGAFIGLGFMLVGKYLIDQRMKARQPKAARPVVLQQGQPLADE
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCH
>Mature Secondary Structure
MNEAKRILLEGLWRNNSSLVQLLGLCPLLAVSNTATNALGLGLATTLVLVCTNTAVSALR
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RWITGEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFIPLIVTNCIVIGRAEAYAS
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHC
QRPPALAALDGLSIGLGSTCTLLLLGALREVLGNGTLFDGADQLLGAWARVLRIEVFHTD
CCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHEEECC
TPFLLAMLPPGAFIGLGFMLVGKYLIDQRMKARQPKAARPVVLQQGQPLADE
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA