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The map label for this gene is nth [H]

Identifier: 85059427

GI number: 85059427

Start: 2423672

End: 2424310

Strand: Reverse

Name: nth [H]

Synonym: SG1449

Alternate gene names: 85059427

Gene position: 2424310-2423672 (Counterclockwise)

Preceding gene: 85059428

Following gene: 85059422

Centisome position: 58.12

GC content: 55.24

Gene sequence:

>639_bases
ATGAATAAAAGCAAGCGTTATGACATTCTGTGTCGCCTGCGGGCGAATAATCCCCATCCCACCACCGAGCTGATGTACCG
CTCGCCTTTCGAGCTGTTGATTGCAGTACTGCTCTCGGCCCAGGCGACCGATGTCAGCGTCAATAAGGCCACCAAACTGT
TATTCCCTGCGGCCGACACGCCTCAGGCCATGCTGGCGCTGGGCGTTGAGGGCGTCAAAGGCTATATCAAATCTATAGGG
CTCTTTAACAGCAAGGCGGAGAACATAATCAAAACCTGCCGGCTGTTGTTGGAACGCCACCAGGGTGAGGTTCCCCAAGA
CCGCACGGCGCTGGAAGCGCTGCCCGGCGTCGGGCGCAAAACCGCCAATGTAGTGTTGAACACTGCTTTCGGCTGGCCGA
CGATTGCGGTTGACACGCATATTTTCCGCGTTGCGAACCGTACCCGCTTTGCGGTGGGTAAAGATGTGGAAGCGGTGGAA
CAAAAGCTGTTGGCCGTGGTACCGGGTGAGTTCAAAGTGGATTGTCACCATTGGTTCATCCTGCACGGCCGCTATACCTG
TATCGCCCGCAAACCACGCTGTGGCTCCTGCCTGATTGAAGATTTGTGTGAGTACGACCAGAAACATTATCCGCAATAA

Upstream 100 bases:

>100_bases
TTTATGCTGGTGGGGAAATACCTTATCGATCAACGCATGAAGGCCCGGCAGCCAAAGGCGGCCCGACCGGTAGTGCTGCA
ACAAGGACAGCCATTAGCCG

Downstream 100 bases:

>100_bases
ACTCCATGCGTTGCGGCGGGGGCCCTCCCGGAACCCACATCATCCCGTCTTTAGGCGCCACCCCAGTCCTGCAGAAAGCC
TGTTTTCTTGCACCCTATGC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNKSKRYDILCRLRANNPHPTTELMYRSPFELLIAVLLSAQATDVSVNKATKLLFPAADTPQAMLALGVEGVKGYIKSIG
LFNSKAENIIKTCRLLLERHQGEVPQDRTALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVANRTRFAVGKDVEAVE
QKLLAVVPGEFKVDCHHWFILHGRYTCIARKPRCGSCLIEDLCEYDQKHYPQ

Sequences:

>Translated_212_residues
MNKSKRYDILCRLRANNPHPTTELMYRSPFELLIAVLLSAQATDVSVNKATKLLFPAADTPQAMLALGVEGVKGYIKSIG
LFNSKAENIIKTCRLLLERHQGEVPQDRTALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVANRTRFAVGKDVEAVE
QKLLAVVPGEFKVDCHHWFILHGRYTCIARKPRCGSCLIEDLCEYDQKHYPQ
>Mature_212_residues
MNKSKRYDILCRLRANNPHPTTELMYRSPFELLIAVLLSAQATDVSVNKATKLLFPAADTPQAMLALGVEGVKGYIKSIG
LFNSKAENIIKTCRLLLERHQGEVPQDRTALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVANRTRFAVGKDVEAVE
QKLLAVVPGEFKVDCHHWFILHGRYTCIARKPRCGSCLIEDLCEYDQKHYPQ

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=181, Percent_Identity=30.939226519337, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=83.1730769230769, Blast_Score=366, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17554540, Length=202, Percent_Identity=28.7128712871287, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324530, Length=187, Percent_Identity=25.1336898395722, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45550361, Length=178, Percent_Identity=29.7752808988764, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23744; Mature: 23744

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSKRYDILCRLRANNPHPTTELMYRSPFELLIAVLLSAQATDVSVNKATKLLFPAADT
CCCCCCEEEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHEEEECCCCC
PQAMLALGVEGVKGYIKSIGLFNSKAENIIKTCRLLLERHQGEVPQDRTALEALPGVGRK
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCC
TANVVLNTAFGWPTIAVDTHIFRVANRTRFAVGKDVEAVEQKLLAVVPGEFKVDCHHWFI
HHHEEEECCCCCCEEEHHHHHHHHHCCCCEECCCCHHHHHHHHHHHCCCCEEEEEEEEEE
LHGRYTCIARKPRCGSCLIEDLCEYDQKHYPQ
EECCEEEEECCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKSKRYDILCRLRANNPHPTTELMYRSPFELLIAVLLSAQATDVSVNKATKLLFPAADT
CCCCCCEEEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHEEEECCCCC
PQAMLALGVEGVKGYIKSIGLFNSKAENIIKTCRLLLERHQGEVPQDRTALEALPGVGRK
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCC
TANVVLNTAFGWPTIAVDTHIFRVANRTRFAVGKDVEAVEQKLLAVVPGEFKVDCHHWFI
HHHEEEECCCCCCEEEHHHHHHHHHCCCCEECCCCHHHHHHHHHHHCCCCEEEEEEEEEE
LHGRYTCIARKPRCGSCLIEDLCEYDQKHYPQ
EECCEEEEECCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]