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The map label for this gene is rnt [H]

Identifier: 85059419

GI number: 85059419

Start: 2415649

End: 2416353

Strand: Reverse

Name: rnt [H]

Synonym: SG1441

Alternate gene names: 85059419

Gene position: 2416353-2415649 (Counterclockwise)

Preceding gene: 85059422

Following gene: 85059416

Centisome position: 57.93

GC content: 58.16

Gene sequence:

>705_bases
ATGGCTGAACAAGACGATTTGACTACCCTCCGCGCCCGCTTTCGCGGCTTTTATCCCGTTGTCATTGATGTGGAAACCGC
AGGCTTTAACGCCGTGACCGATGCCCTGCTGGAAATCGCCGCCGTAACCTTGAAGATGGATGACGATGGCTGGTTGCAGG
CTGACTCCACTCTACATTTCCATGTTGATCCCTTTCCCGGCGCGCTGCTGCAGCCGGAAGCGCTGGCCTTTAACGGTATC
GATCCGCACAATCCGCTCCGCGGCGCGGTTGGCGAGTATGAGGCCCTGCATGAAATTTTTAAAATGGTGCGTAAGGGGAT
TAAAGATCAAGGCTGCAACCGCGCCATTATCGTCGCCCACAACGCCGCTTTCGATCATGGCTTCTTAATGGCCGCAGTCT
CACGCGCCGGTTTAAAACGCAACCCTTTCCATCCCTTCGCGACCTTTGATACGGCGGCGCTGAGCGGCCTGGTGCTCGGC
CAGACCGTGTTGGCCAAAGCCTGCGCCGCGGCCGGCATCGCTTTTAACAGCGGCGAAGCGCATTCTGCGCTGTATGACAC
CGAACGTACCGCGACGCTGTTTTGTGAATTGGTCAACCGTTGGAAACGTCTTGGCGGCTGGCCGGTCGCAATGCCCGCAC
CGGGCGATGCCGAAGAAGAGGCGGTCGCCGCACAAGATCGCGCCAACCCGACTCTCGATTTATAA

Upstream 100 bases:

>100_bases
TCACGGCCCGTTCTTCCCGCCCCGTCTGCATAACTCACCATGCAAGACGTACCAAAATTTGCCATAATAGCGGCTGAATT
CGCTGAACATAAGAACAGGC

Downstream 100 bases:

>100_bases
AGCTTCAGCCCGGCGGCATTGCGCGCAAAAAAAAGGCCATCCGAGGCGTCGGGTGGCCTTTTTGCAGCGGTTAAGAAATC
TGTACCGCGTTCGCTACGCC

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MAEQDDLTTLRARFRGFYPVVIDVETAGFNAVTDALLEIAAVTLKMDDDGWLQADSTLHFHVDPFPGALLQPEALAFNGI
DPHNPLRGAVGEYEALHEIFKMVRKGIKDQGCNRAIIVAHNAAFDHGFLMAAVSRAGLKRNPFHPFATFDTAALSGLVLG
QTVLAKACAAAGIAFNSGEAHSALYDTERTATLFCELVNRWKRLGGWPVAMPAPGDAEEEAVAAQDRANPTLDL

Sequences:

>Translated_234_residues
MAEQDDLTTLRARFRGFYPVVIDVETAGFNAVTDALLEIAAVTLKMDDDGWLQADSTLHFHVDPFPGALLQPEALAFNGI
DPHNPLRGAVGEYEALHEIFKMVRKGIKDQGCNRAIIVAHNAAFDHGFLMAAVSRAGLKRNPFHPFATFDTAALSGLVLG
QTVLAKACAAAGIAFNSGEAHSALYDTERTATLFCELVNRWKRLGGWPVAMPAPGDAEEEAVAAQDRANPTLDL
>Mature_233_residues
AEQDDLTTLRARFRGFYPVVIDVETAGFNAVTDALLEIAAVTLKMDDDGWLQADSTLHFHVDPFPGALLQPEALAFNGID
PHNPLRGAVGEYEALHEIFKMVRKGIKDQGCNRAIIVAHNAAFDHGFLMAAVSRAGLKRNPFHPFATFDTAALSGLVLGQ
TVLAKACAAAGIAFNSGEAHSALYDTERTATLFCELVNRWKRLGGWPVAMPAPGDAEEEAVAAQDRANPTLDL

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=210, Percent_Identity=78.0952380952381, Blast_Score=346, Evalue=8e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 25214; Mature: 25083

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQDDLTTLRARFRGFYPVVIDVETAGFNAVTDALLEIAAVTLKMDDDGWLQADSTLHF
CCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHEEEECCCCCEEECCEEEE
HVDPFPGALLQPEALAFNGIDPHNPLRGAVGEYEALHEIFKMVRKGIKDQGCNRAIIVAH
EECCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
NAAFDHGFLMAAVSRAGLKRNPFHPFATFDTAALSGLVLGQTVLAKACAAAGIAFNSGEA
CCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCC
HSALYDTERTATLFCELVNRWKRLGGWPVAMPAPGDAEEEAVAAQDRANPTLDL
HHHHHCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AEQDDLTTLRARFRGFYPVVIDVETAGFNAVTDALLEIAAVTLKMDDDGWLQADSTLHF
CCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHEEEECCCCCEEECCEEEE
HVDPFPGALLQPEALAFNGIDPHNPLRGAVGEYEALHEIFKMVRKGIKDQGCNRAIIVAH
EECCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
NAAFDHGFLMAAVSRAGLKRNPFHPFATFDTAALSGLVLGQTVLAKACAAAGIAFNSGEA
CCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCC
HSALYDTERTATLFCELVNRWKRLGGWPVAMPAPGDAEEEAVAAQDRANPTLDL
HHHHHCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA