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The map label for this gene is pykF [H]

Identifier: 85059416

GI number: 85059416

Start: 2405862

End: 2407274

Strand: Reverse

Name: pykF [H]

Synonym: SG1438

Alternate gene names: 85059416

Gene position: 2407274-2405862 (Counterclockwise)

Preceding gene: 85059419

Following gene: 85059415

Centisome position: 57.71

GC content: 54.85

Gene sequence:

>1413_bases
ATGAAAAAGACTAAAATTGTTTGTACCATCGGCCCGAAAACCGAATCCGAAGAGGTACTGTCCAGCCTGCTTGATGCAGG
TATGAACGTCATGCGTCTCAATTTTTCCCATGGTGATTATGAAGAGCACGGTCAGCGCATCAAAAATCTGCGTGCGGTGT
TGAAAAGAACCGATCAGCAGGCCGCGATTCTGCTTGATACTAAGGGCCCCGAAATCCGCACCATGAAATTGGTAAACGGC
GCCGATGTCTCGCTGCGCGCCGGCCAAACCTTTACCTTTACGACCGATCAGAGCATTATTGGCAACAGTGAACGCGTTGC
GGTCACCTATCCGGGGTTCACCCAGGATCTGGCGGTAGGTAATACCATTCTGGTCGATGACGGTCTGCTGGGCATGACCG
TGACCAGCGTCACCCAGGACCAGGTTGTATGTAAGGTACTCAACAATGGCGATTTGGGTGAAAACAAAGGGGTTAACCTG
CCCGGTGTGTCCATTCCCCTACCCGCGCTGGCGGAAAAAGATAAACGCGACTTGATTTTTGGCTGCGAACAAGGCGTCGA
TTTTATCGCTGCCTCCTTTATTCGCAAACGTTCCGACGTACTGGAAATCCGCGAATACCTGGAACAGCACGGCGGCAGCC
ATATCCAGATCATCTCTAAAATCGAAAACCAGGAAGGGCTGAATAATTTCGACGAAATCCTCGAAGCCTCCGACGGCATT
ATGGTAGCCCGCGGCGATCTCGGTGTCGAAATTCCGGTGGAAGAAGTGATTTTCGCGCAGAAAATGATGATCGAAAAGTG
TAACCGGGCGCGCAAGGTGGTGATTACCGCGACCCAAATGCTCGACTCGATGATCAAAAACCCGCGTCCGACCCGCGCGG
AAGCCGGCGACGTGGCCAACGCCATTCTGGATGGTACGGACGCCGTGATGCTGTCCGGCGAGAGCGCCAAAGGTAAATAC
CCGCTGGAAGCCGTGACCATTATGGCCACTATCTGCCAGCGCACCGATCGAATGCTGCCGAGCCGCATCGACAGCCTGAA
CGATAGCCGCAAACTTCGCATTACCGAAGCGGTCTGCCGCAGCGCGGTCGAAACCGCAGAAAAACTGGAATCGCCGCTGA
TTGTAGTCGCGACCGAGGGCGGCAAATCCGCCAAGTCAGTGCGTAAATATTTCCCGAAAGCGGAAATTCTGGCCCTGACC
ACGAACCCGCTGACCGCCCGCCAGCTTCTGCTGAGCAAAGGGGTGTCCTGCCAGCTTGTCGAAGAAATCGCGTCGACCGA
TGATTTCTACCGCATCGGCAAGGCAATGGCGTTGGAAACTGGTTTAGCGGCCAAGGGCGACATTATCGTCATGGTGTCCG
GCGCGCTGGTACCGAGCGGCACCACCAACACCGCCTCGGTGCATATGCTGTAA

Upstream 100 bases:

>100_bases
AAAATGGACCGCAGGTTTGAAGCGGCTTTTTTTTCATCGTGGGCCTGGTCCGGTGATGCACCAGCTTTATCATTCATCAC
AAATTATAGAAATCAACGTT

Downstream 100 bases:

>100_bases
CGCAGGCCGGGGGCAGGCATGTCTGTCTCCCTATTCTTGCGGCACTGCACGACGTAGCGCCGCCGCTGGCGCGGTGCGCT
TAGAGCAGATCCAGCTCATC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-1 [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MKKTKIVCTIGPKTESEEVLSSLLDAGMNVMRLNFSHGDYEEHGQRIKNLRAVLKRTDQQAAILLDTKGPEIRTMKLVNG
ADVSLRAGQTFTFTTDQSIIGNSERVAVTYPGFTQDLAVGNTILVDDGLLGMTVTSVTQDQVVCKVLNNGDLGENKGVNL
PGVSIPLPALAEKDKRDLIFGCEQGVDFIAASFIRKRSDVLEIREYLEQHGGSHIQIISKIENQEGLNNFDEILEASDGI
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKY
PLEAVTIMATICQRTDRMLPSRIDSLNDSRKLRITEAVCRSAVETAEKLESPLIVVATEGGKSAKSVRKYFPKAEILALT
TNPLTARQLLLSKGVSCQLVEEIASTDDFYRIGKAMALETGLAAKGDIIVMVSGALVPSGTTNTASVHML

Sequences:

>Translated_470_residues
MKKTKIVCTIGPKTESEEVLSSLLDAGMNVMRLNFSHGDYEEHGQRIKNLRAVLKRTDQQAAILLDTKGPEIRTMKLVNG
ADVSLRAGQTFTFTTDQSIIGNSERVAVTYPGFTQDLAVGNTILVDDGLLGMTVTSVTQDQVVCKVLNNGDLGENKGVNL
PGVSIPLPALAEKDKRDLIFGCEQGVDFIAASFIRKRSDVLEIREYLEQHGGSHIQIISKIENQEGLNNFDEILEASDGI
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKY
PLEAVTIMATICQRTDRMLPSRIDSLNDSRKLRITEAVCRSAVETAEKLESPLIVVATEGGKSAKSVRKYFPKAEILALT
TNPLTARQLLLSKGVSCQLVEEIASTDDFYRIGKAMALETGLAAKGDIIVMVSGALVPSGTTNTASVHML
>Mature_470_residues
MKKTKIVCTIGPKTESEEVLSSLLDAGMNVMRLNFSHGDYEEHGQRIKNLRAVLKRTDQQAAILLDTKGPEIRTMKLVNG
ADVSLRAGQTFTFTTDQSIIGNSERVAVTYPGFTQDLAVGNTILVDDGLLGMTVTSVTQDQVVCKVLNNGDLGENKGVNL
PGVSIPLPALAEKDKRDLIFGCEQGVDFIAASFIRKRSDVLEIREYLEQHGGSHIQIISKIENQEGLNNFDEILEASDGI
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKY
PLEAVTIMATICQRTDRMLPSRIDSLNDSRKLRITEAVCRSAVETAEKLESPLIVVATEGGKSAKSVRKYFPKAEILALT
TNPLTARQLLLSKGVSCQLVEEIASTDDFYRIGKAMALETGLAAKGDIIVMVSGALVPSGTTNTASVHML

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=487, Percent_Identity=47.8439425051335, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI33286420, Length=487, Percent_Identity=47.8439425051335, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI33286418, Length=488, Percent_Identity=48.155737704918, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI10835121, Length=488, Percent_Identity=45.4918032786885, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI32967597, Length=488, Percent_Identity=45.4918032786885, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=51.2110726643599, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=51.2110726643599, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=45.9915611814346, Blast_Score=191, Evalue=9e-49,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=45.9915611814346, Blast_Score=191, Evalue=9e-49,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=45.8333333333333, Blast_Score=171, Evalue=2e-42,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=83.1914893617021, Blast_Score=811, Evalue=0.0,
Organism=Escherichia coli, GI1788160, Length=481, Percent_Identity=36.5904365904366, Blast_Score=265, Evalue=6e-72,
Organism=Caenorhabditis elegans, GI17544584, Length=479, Percent_Identity=41.9624217118998, Blast_Score=363, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17506829, Length=480, Percent_Identity=46.0416666666667, Blast_Score=357, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI71984413, Length=480, Percent_Identity=46.0416666666667, Blast_Score=356, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI71984406, Length=480, Percent_Identity=46.0416666666667, Blast_Score=356, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI17506831, Length=480, Percent_Identity=46.0416666666667, Blast_Score=355, Evalue=2e-98,
Organism=Saccharomyces cerevisiae, GI6319279, Length=481, Percent_Identity=45.3222453222453, Blast_Score=353, Evalue=2e-98,
Organism=Saccharomyces cerevisiae, GI6324923, Length=483, Percent_Identity=43.2712215320911, Blast_Score=339, Evalue=5e-94,
Organism=Drosophila melanogaster, GI28571814, Length=480, Percent_Identity=47.0833333333333, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24648964, Length=480, Percent_Identity=47.0833333333333, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24648966, Length=446, Percent_Identity=45.9641255605381, Blast_Score=344, Evalue=6e-95,
Organism=Drosophila melanogaster, GI24581235, Length=482, Percent_Identity=36.9294605809129, Blast_Score=289, Evalue=3e-78,
Organism=Drosophila melanogaster, GI24646914, Length=301, Percent_Identity=45.514950166113, Blast_Score=210, Evalue=2e-54,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50897; Mature: 50897

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTKIVCTIGPKTESEEVLSSLLDAGMNVMRLNFSHGDYEEHGQRIKNLRAVLKRTDQQ
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCC
AAILLDTKGPEIRTMKLVNGADVSLRAGQTFTFTTDQSIIGNSERVAVTYPGFTQDLAVG
EEEEEECCCCCEEEEEEECCCCEEEECCCEEEEECCCHHCCCCCEEEEECCCCCHHHHCC
NTILVDDGLLGMTVTSVTQDQVVCKVLNNGDLGENKGVNLPGVSIPLPALAEKDKRDLIF
CEEEEECCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCEEE
GCEQGVDFIAASFIRKRSDVLEIREYLEQHGGSHIQIISKIENQEGLNNFDEILEASDGI
ECHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHCCCCCCHHHHHHHCCCCE
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVAN
EEEECCCCCCCCHHHHHHHHHHHHHHHCCHHEEEEEHHHHHHHHHCCCCCCCCCHHHHHH
AILDGTDAVMLSGESAKGKYPLEAVTIMATICQRTDRMLPSRIDSLNDSRKLRITEAVCR
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHH
SAVETAEKLESPLIVVATEGGKSAKSVRKYFPKAEILALTTNPLTARQLLLSKGVSCQLV
HHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCHHHH
EEIASTDDFYRIGKAMALETGLAAKGDIIVMVSGALVPSGTTNTASVHML
HHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCEEEEC
>Mature Secondary Structure
MKKTKIVCTIGPKTESEEVLSSLLDAGMNVMRLNFSHGDYEEHGQRIKNLRAVLKRTDQQ
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCC
AAILLDTKGPEIRTMKLVNGADVSLRAGQTFTFTTDQSIIGNSERVAVTYPGFTQDLAVG
EEEEEECCCCCEEEEEEECCCCEEEECCCEEEEECCCHHCCCCCEEEEECCCCCHHHHCC
NTILVDDGLLGMTVTSVTQDQVVCKVLNNGDLGENKGVNLPGVSIPLPALAEKDKRDLIF
CEEEEECCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCEEE
GCEQGVDFIAASFIRKRSDVLEIREYLEQHGGSHIQIISKIENQEGLNNFDEILEASDGI
ECHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHCCCCCCHHHHHHHCCCCE
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVAN
EEEECCCCCCCCHHHHHHHHHHHHHHHCCHHEEEEEHHHHHHHHHCCCCCCCCCHHHHHH
AILDGTDAVMLSGESAKGKYPLEAVTIMATICQRTDRMLPSRIDSLNDSRKLRITEAVCR
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHH
SAVETAEKLESPLIVVATEGGKSAKSVRKYFPKAEILALTTNPLTARQLLLSKGVSCQLV
HHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCHHHH
EEIASTDDFYRIGKAMALETGLAAKGDIIVMVSGALVPSGTTNTASVHML
HHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]