Click here to switch to the map view.

The map label for this gene is trpD [H]

Identifier: 85059379

GI number: 85059379

Start: 2325443

End: 2326021

Strand: Reverse

Name: trpD [H]

Synonym: SG1401

Alternate gene names: 85059379

Gene position: 2326021-2325443 (Counterclockwise)

Preceding gene: 85059380

Following gene: 85059378

Centisome position: 55.76

GC content: 59.41

Gene sequence:

>579_bases
ATGGCCGACATCCTGTTACTCGATAATATCGACTCGTTTACCTATAACCTGGTGGATCAGCTGCGCGCCAACGGTCACCG
AGTAGTGATTTACCGCAACCGGCTACCGGCCGCGCTCATCACGCGGGCGCTGTCCGAAATGGAAAACCCGATCCTGATGC
TCTCGCCAGGCCCCGGTGCGCCCGCTCAGGCGGGCTGCATGCCAGCATTGCTCACGACGTTGCGTGGCAGGTTACCCATC
ATCGGCATCTGCCTGGGCCATCAGGCGATTGTGGAAGCTTACGGCGGCCACGTCGGCCAGGCGGGCGAGATTTTGCACGG
TAAAGCCTCGCCCATCCGCCACGATAACCAGGGCATGTTCGCCGGTCTGCCGAACCCGCTGCCAGTGGCGCGCTATCATT
CGCTGGTCGGGAATCAAGTGCCGGCAACGCTCACGGTCAATGCCCACTTTAACGACATGGTGATGGCGGTACGCAATGAT
GCCGATCGGATCTGCGGCTTTCAGTTTCACCCTGAATCTATTTTAACCACCCAGGGAGCACATTTGTTAAACCAGACGCT
CGCCTGGGCACTGGCGTAA

Upstream 100 bases:

>100_bases
TGGATTCCGTTCCACAGGCAGAAGCCGACGAAAGCCGCAACAAAGCGCGTGCCGTTTTGCGCGCCATTGCTACCGCCCAC
GACGCAAAGGAGCTGTTCTG

Downstream 100 bases:

>100_bases
TCAGAAGGACTTTTTCATGCAAGCGATACTGGAACATCTCTATCAGGGCGGGCGTATCAGCCGCGAACAAAGCCAGCAGC
TGTTCGGGGCCATCATTCAA

Product: anthranilate synthase component II

Products: NA

Alternate protein names: Glutamine amidotransferase; Anthranilate phosphoribosyltransferase [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MADILLLDNIDSFTYNLVDQLRANGHRVVIYRNRLPAALITRALSEMENPILMLSPGPGAPAQAGCMPALLTTLRGRLPI
IGICLGHQAIVEAYGGHVGQAGEILHGKASPIRHDNQGMFAGLPNPLPVARYHSLVGNQVPATLTVNAHFNDMVMAVRND
ADRICGFQFHPESILTTQGAHLLNQTLAWALA

Sequences:

>Translated_192_residues
MADILLLDNIDSFTYNLVDQLRANGHRVVIYRNRLPAALITRALSEMENPILMLSPGPGAPAQAGCMPALLTTLRGRLPI
IGICLGHQAIVEAYGGHVGQAGEILHGKASPIRHDNQGMFAGLPNPLPVARYHSLVGNQVPATLTVNAHFNDMVMAVRND
ADRICGFQFHPESILTTQGAHLLNQTLAWALA
>Mature_191_residues
ADILLLDNIDSFTYNLVDQLRANGHRVVIYRNRLPAALITRALSEMENPILMLSPGPGAPAQAGCMPALLTTLRGRLPII
GICLGHQAIVEAYGGHVGQAGEILHGKASPIRHDNQGMFAGLPNPLPVARYHSLVGNQVPATLTVNAHFNDMVMAVRNDA
DRICGFQFHPESILTTQGAHLLNQTLAWALA

Specific function: Tryptophan biosynthesis; first step. Tryptophan biosynthesis; second step. [C]

COG id: COG0512

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787517, Length=190, Percent_Identity=80.5263157894737, Blast_Score=326, Evalue=8e-91,
Organism=Escherichia coli, GI1789760, Length=187, Percent_Identity=43.8502673796791, Blast_Score=148, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6322638, Length=184, Percent_Identity=41.8478260869565, Blast_Score=151, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6324361, Length=209, Percent_Identity=25.3588516746411, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 945 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005940
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR000312
- InterPro:   IPR017459
- InterPro:   IPR020072
- InterPro:   IPR006221 [H]

Pfam domain/function: PF00117 GATase; PF02885 Glycos_trans_3N; PF00591 Glycos_transf_3 [H]

EC number: =4.1.3.27; =2.4.2.18 [H]

Molecular weight: Translated: 20680; Mature: 20549

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADILLLDNIDSFTYNLVDQLRANGHRVVIYRNRLPAALITRALSEMENPILMLSPGPGA
CCCEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCC
PAQAGCMPALLTTLRGRLPIIGICLGHQAIVEAYGGHVGQAGEILHGKASPIRHDNQGMF
CCCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHCCCCCCCCCEECCCCCCCEECCCCCE
AGLPNPLPVARYHSLVGNQVPATLTVNAHFNDMVMAVRNDADRICGFQFHPESILTTQGA
ECCCCCCCHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCHHHHCCCEECCCHHHHHCCH
HLLNQTLAWALA
HHHHHHHHHHCC
>Mature Secondary Structure 
ADILLLDNIDSFTYNLVDQLRANGHRVVIYRNRLPAALITRALSEMENPILMLSPGPGA
CCEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCC
PAQAGCMPALLTTLRGRLPIIGICLGHQAIVEAYGGHVGQAGEILHGKASPIRHDNQGMF
CCCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHCCCCCCCCCEECCCCCCCEECCCCCE
AGLPNPLPVARYHSLVGNQVPATLTVNAHFNDMVMAVRNDADRICGFQFHPESILTTQGA
ECCCCCCCHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCHHHHCCCEECCCHHHHHCCH
HLLNQTLAWALA
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7038627; 6283099; 9097039; 9278503; 4594441 [H]