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The map label for this gene is sppA [H]

Identifier: 85059328

GI number: 85059328

Start: 2243432

End: 2245282

Strand: Reverse

Name: sppA [H]

Synonym: SG1350

Alternate gene names: 85059328

Gene position: 2245282-2243432 (Counterclockwise)

Preceding gene: 85059337

Following gene: 85059325

Centisome position: 53.83

GC content: 55.59

Gene sequence:

>1851_bases
ATGCGCACATTGTGGCGAATTATTTCCGGCCCGTTCAGATGGTGCTGGCATTTACTGAATTTCATCCGTGAATTTATTCT
TAACGTATTTTTGGTGGTTCTCATCATCGTCGTCGGGATCTATTTACAAATGCGTCACACACCGCCGACGCTGGCAAAAG
GCGCGCTGGTGATGGATCTGACCGGTACGGTGGTAGACAAGCCGGCCATGAATAATAAATTCCGTCAGCTTGGCCGCGAA
TTGCTGGGCGCTTCCAGCAACCGGCTTCAGGAAAATTCGCTGTTTGACGTGGTGGATACGCTACGCCAGGCGAAAGGCGA
CGCCAATATCACTGGGCTGGTCTTGTCGTTGAAAGATTTTGCCGGTGCGGACCAGGCTTCGCTGGAGTACATCGGCAAGG
CGCTGCGCGAATTCCGCGATAGCAGTAAACCTATCTATGCCGTCGGCGACAGCTATAGCCAGGCGCAATATTATCTTGCC
AGCTATGCCAATAAAATCTATCTCACGCCCCAGGGAGCGGTGGATTTACACGGCATAGCAACGAATAACCTTTACTATAA
AACCTTACTCGACAAGCTAAAAGTCAATAGCCATATCTTCCGCGTCGGCACCTATAAATCCGCGGTCGAACCTTTCCTGC
GCGACAATATGTCCGACGACGCCCGCGAAGCCGATAGCCGGTGGGTGGGTCAACTGTGGCAGCACTATTTGACGACGGTG
GCGACCAATCGGCAAACCACGCCGGCGCAATTGTTCCCGGGCGCCCAGGCAATGTTGGATGATCTGCGCGCGGTCGAGGG
CAATACTGCACAATTCGCGCTCAATAACAAATGGGTGGATGAAGTAGCGTCCCGCTCGGCGATTGACGCGGCCCTGACCA
AGGCGTTTGGCTGGAACAAGCGCGACAAGGCGTTTAATGGCATCAGTATTTATGATTATCAGCCGGTGACGTCGACGCAA
CAGGGCGGTCAGATCGCCGTTATTTTCGCCAATGGCGCCATCATCGATGGTCCGGAAACGCCAGGATCGGTCGGGGGCGA
CACTACCGCCGACCAAATCCGCGATGCGCGCATGGATCCCGACATCAAGGCCATTGTCTTCCGGGTCAACAGCCCAGGCG
GCAGCGTTACTGCCTCCGAGGTCATTCGTTCCGAGCTGGCCGCCGCGCGCGCCGCAGGTAAACCGGTGGTGATTTCTATG
GGCGGTATAGCGGCGTCAGGCGGTTATTGGGTGTCCACTCCCGCCAATTACATCATCGCCAGCGCCAGCACCTTGACCGG
GTCGATCGGCATCTTCGGGGTGGTTAATACCGTTGAGAACTCCCTCGACAGCATCGGCGTGCACACCGATGGAGTTGCCA
CTTCGCCGCTGGCGGATGTGTCGCTGACCAAAACGCTGCCGGCGGAATTCTCGCAGATGATGCAACTTAGCGTAGAAAAC
GGTTATCGCAACTTTATCACTCTGGTCGCGCAATCCCGCCACAAAACCCCAGAGGAGATAGACAAAATCGGCCAGGGCCA
TGTCTGGATTGGCAGCGATGCGGTTCAAAACGGGCTGGTCGATCGAGTGGGTGATTTCGACGATGCCGTCAGTAAAGCCG
CTGAACTAGCGCAGTTGAAGCAATACCAATTGAACTGGTATCTGGATGATCCGGGCCTGTGGAATTTACTGTTCACGCAG
ACCAGCGCCTCGGTGTATGCCTTACTGCCGCAGCTTATCCAGTCCTGGCTGCCGGCGCCGGTCACCCAAGTGGTGGACAG
GTTGAAGATGGATGCCGGCCTGGGCTTTAACTGGAACGATCCGCAAAATCGTTACGCCTTTTGCCTTTCCTGCGAGCAAA
TCTTGCGCTAG

Upstream 100 bases:

>100_bases
TATCGGCGCGCTGGTAAGCTTTACCTGAAGCGTTTTTTAGAGCGCGGCCGGACAAATTTTATATTATGATTTTGAACTTC
GCTGTTTTATTGGAGATCCC

Downstream 100 bases:

>100_bases
GGCGTTACCTGCGACCGTCCTGGTGCCCCGGACGCCGGGCCACCTGAAACAACGCCCCGCCGAGGCGATATGACTGTCGA
GGCTGCCCTGACCAAGCTAC

Product: protease 4

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase [H]

Number of amino acids: Translated: 616; Mature: 616

Protein sequence:

>616_residues
MRTLWRIISGPFRWCWHLLNFIREFILNVFLVVLIIVVGIYLQMRHTPPTLAKGALVMDLTGTVVDKPAMNNKFRQLGRE
LLGASSNRLQENSLFDVVDTLRQAKGDANITGLVLSLKDFAGADQASLEYIGKALREFRDSSKPIYAVGDSYSQAQYYLA
SYANKIYLTPQGAVDLHGIATNNLYYKTLLDKLKVNSHIFRVGTYKSAVEPFLRDNMSDDAREADSRWVGQLWQHYLTTV
ATNRQTTPAQLFPGAQAMLDDLRAVEGNTAQFALNNKWVDEVASRSAIDAALTKAFGWNKRDKAFNGISIYDYQPVTSTQ
QGGQIAVIFANGAIIDGPETPGSVGGDTTADQIRDARMDPDIKAIVFRVNSPGGSVTASEVIRSELAAARAAGKPVVISM
GGIAASGGYWVSTPANYIIASASTLTGSIGIFGVVNTVENSLDSIGVHTDGVATSPLADVSLTKTLPAEFSQMMQLSVEN
GYRNFITLVAQSRHKTPEEIDKIGQGHVWIGSDAVQNGLVDRVGDFDDAVSKAAELAQLKQYQLNWYLDDPGLWNLLFTQ
TSASVYALLPQLIQSWLPAPVTQVVDRLKMDAGLGFNWNDPQNRYAFCLSCEQILR

Sequences:

>Translated_616_residues
MRTLWRIISGPFRWCWHLLNFIREFILNVFLVVLIIVVGIYLQMRHTPPTLAKGALVMDLTGTVVDKPAMNNKFRQLGRE
LLGASSNRLQENSLFDVVDTLRQAKGDANITGLVLSLKDFAGADQASLEYIGKALREFRDSSKPIYAVGDSYSQAQYYLA
SYANKIYLTPQGAVDLHGIATNNLYYKTLLDKLKVNSHIFRVGTYKSAVEPFLRDNMSDDAREADSRWVGQLWQHYLTTV
ATNRQTTPAQLFPGAQAMLDDLRAVEGNTAQFALNNKWVDEVASRSAIDAALTKAFGWNKRDKAFNGISIYDYQPVTSTQ
QGGQIAVIFANGAIIDGPETPGSVGGDTTADQIRDARMDPDIKAIVFRVNSPGGSVTASEVIRSELAAARAAGKPVVISM
GGIAASGGYWVSTPANYIIASASTLTGSIGIFGVVNTVENSLDSIGVHTDGVATSPLADVSLTKTLPAEFSQMMQLSVEN
GYRNFITLVAQSRHKTPEEIDKIGQGHVWIGSDAVQNGLVDRVGDFDDAVSKAAELAQLKQYQLNWYLDDPGLWNLLFTQ
TSASVYALLPQLIQSWLPAPVTQVVDRLKMDAGLGFNWNDPQNRYAFCLSCEQILR
>Mature_616_residues
MRTLWRIISGPFRWCWHLLNFIREFILNVFLVVLIIVVGIYLQMRHTPPTLAKGALVMDLTGTVVDKPAMNNKFRQLGRE
LLGASSNRLQENSLFDVVDTLRQAKGDANITGLVLSLKDFAGADQASLEYIGKALREFRDSSKPIYAVGDSYSQAQYYLA
SYANKIYLTPQGAVDLHGIATNNLYYKTLLDKLKVNSHIFRVGTYKSAVEPFLRDNMSDDAREADSRWVGQLWQHYLTTV
ATNRQTTPAQLFPGAQAMLDDLRAVEGNTAQFALNNKWVDEVASRSAIDAALTKAFGWNKRDKAFNGISIYDYQPVTSTQ
QGGQIAVIFANGAIIDGPETPGSVGGDTTADQIRDARMDPDIKAIVFRVNSPGGSVTASEVIRSELAAARAAGKPVVISM
GGIAASGGYWVSTPANYIIASASTLTGSIGIFGVVNTVENSLDSIGVHTDGVATSPLADVSLTKTLPAEFSQMMQLSVEN
GYRNFITLVAQSRHKTPEEIDKIGQGHVWIGSDAVQNGLVDRVGDFDDAVSKAAELAQLKQYQLNWYLDDPGLWNLLFTQ
TSASVYALLPQLIQSWLPAPVTQVVDRLKMDAGLGFNWNDPQNRYAFCLSCEQILR

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=617, Percent_Identity=65.3160453808752, Blast_Score=834, Evalue=0.0,
Organism=Escherichia coli, GI1787527, Length=175, Percent_Identity=26.2857142857143, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 67510; Mature: 67510

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTLWRIISGPFRWCWHLLNFIREFILNVFLVVLIIVVGIYLQMRHTPPTLAKGALVMDL
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCEEEEE
TGTVVDKPAMNNKFRQLGRELLGASSNRLQENSLFDVVDTLRQAKGDANITGLVLSLKDF
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEHHH
AGADQASLEYIGKALREFRDSSKPIYAVGDSYSQAQYYLASYANKIYLTPQGAVDLHGIA
CCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCEEEECCCCCEEEEEEE
TNNLYYKTLLDKLKVNSHIFRVGTYKSAVEPFLRDNMSDDAREADSRWVGQLWQHYLTTV
ECCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ATNRQTTPAQLFPGAQAMLDDLRAVEGNTAQFALNNKWVDEVASRSAIDAALTKAFGWNK
HCCCCCCCHHHCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCC
RDKAFNGISIYDYQPVTSTQQGGQIAVIFANGAIIDGPETPGSVGGDTTADQIRDARMDP
CCCCCCCEEEEECCCCCCCCCCCEEEEEEECCEEECCCCCCCCCCCCCCHHHHHHHCCCC
DIKAIVFRVNSPGGSVTASEVIRSELAAARAAGKPVVISMGGIAASGGYWVSTPANYIIA
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCEEEECCCCEEEE
SASTLTGSIGIFGVVNTVENSLDSIGVHTDGVATSPLADVSLTKTLPAEFSQMMQLSVEN
ECHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHC
GYRNFITLVAQSRHKTPEEIDKIGQGHVWIGSDAVQNGLVDRVGDFDDAVSKAAELAQLK
CHHHHHHHHHHHCCCCHHHHHHCCCCCEEECCHHHHCCHHHHCCCHHHHHHHHHHHHHHH
QYQLNWYLDDPGLWNLLFTQTSASVYALLPQLIQSWLPAPVTQVVDRLKMDAGLGFNWND
HHEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC
PQNRYAFCLSCEQILR
CCCCEEEEEEHHHHHC
>Mature Secondary Structure
MRTLWRIISGPFRWCWHLLNFIREFILNVFLVVLIIVVGIYLQMRHTPPTLAKGALVMDL
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCEEEEE
TGTVVDKPAMNNKFRQLGRELLGASSNRLQENSLFDVVDTLRQAKGDANITGLVLSLKDF
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEHHH
AGADQASLEYIGKALREFRDSSKPIYAVGDSYSQAQYYLASYANKIYLTPQGAVDLHGIA
CCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCEEEECCCCCEEEEEEE
TNNLYYKTLLDKLKVNSHIFRVGTYKSAVEPFLRDNMSDDAREADSRWVGQLWQHYLTTV
ECCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ATNRQTTPAQLFPGAQAMLDDLRAVEGNTAQFALNNKWVDEVASRSAIDAALTKAFGWNK
HCCCCCCCHHHCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCC
RDKAFNGISIYDYQPVTSTQQGGQIAVIFANGAIIDGPETPGSVGGDTTADQIRDARMDP
CCCCCCCEEEEECCCCCCCCCCCEEEEEEECCEEECCCCCCCCCCCCCCHHHHHHHCCCC
DIKAIVFRVNSPGGSVTASEVIRSELAAARAAGKPVVISMGGIAASGGYWVSTPANYIIA
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCEEEECCCCEEEE
SASTLTGSIGIFGVVNTVENSLDSIGVHTDGVATSPLADVSLTKTLPAEFSQMMQLSVEN
ECHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHC
GYRNFITLVAQSRHKTPEEIDKIGQGHVWIGSDAVQNGLVDRVGDFDDAVSKAAELAQLK
CHHHHHHHHHHHCCCCHHHHHHCCCCCEEECCHHHHCCHHHHCCCHHHHHHHHHHHHHHH
QYQLNWYLDDPGLWNLLFTQTSASVYALLPQLIQSWLPAPVTQVVDRLKMDAGLGFNWND
HHEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC
PQNRYAFCLSCEQILR
CCCCEEEEEEHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]