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The map label for this gene is purB [H]

Identifier: 85059061

GI number: 85059061

Start: 1791766

End: 1793136

Strand: Reverse

Name: purB [H]

Synonym: SG1083

Alternate gene names: 85059061

Gene position: 1793136-1791766 (Counterclockwise)

Preceding gene: 85059062

Following gene: 85059060

Centisome position: 42.99

GC content: 52.44

Gene sequence:

>1371_bases
ATGGAATTATCCTCTCTGACCGCCGTTTCGCCTATAGACGGCCGCTACGGCGACAAAGTCAGTCCGCTACGCGCTATTTT
CAGTGAATTCGGTTTGCTGAAATTTCGCGTACAGGTGGAAGTACGCTGGCTGCAAAAGCTGGCCGCCTTTGACGCTATTA
AAGAGGTCCCCCCTTTTGACGACCACGCAAACGGTTTCCTTGACGCCATTGTCGCGAACTTCAGCGTAGAGGATGCCGAG
CGCATCAAAATCATTGAACGTACCACCAATCATGATGTGAAAGCGGTAGAATATTTTTTGAAGGAGAAGGTCGCCGGGCA
ACCGGCACTGCAGGCGGTAAGTGAATTCATCCATTTCGCCTGCACCTCCGAGGATATCAACAATCTTTCCCACGCGCTGA
TGCTAGCCACCGCCCGCGAATCCGTTCTGCTACCGGCCTGGCGCGAGATTATCGCGGCGGTAACGGCGCTGGCCGAACGC
TATCGCGATATCCCCCTGCTATCGCGCACCCACGGACAGCCGGCGACGCCCTCAACTATGGGCAAAGAAGTGGCCAATGT
TTCCTATCGTATGACGCGGCAATATCGTCAGTTGGAACGAGTAGAGATACTGGGCAAAATTAACGGCGCCGTCGGCAATT
ATAATGCCCATATGGTCGCCTATCCCGAAGTGAACTGGCATCGGTTCAGCGAGGAGTTCGTCACCGGGCTCGGTATTGGC
TGGAACCCCTATACTACTCAAATCGAGCCGCATGATTATATCGCCGAAATGTTTGACTGCATTGCGCGGTTCAATACTAT
CGTTATCGATTTTGACCGTGACGTGTGGGGTTATATCGCCCTCAATCATTTCAAACAGCGCACCGTCGCCGGCGAAATCG
GTTCTTCGACCATGCCGCATAAAGTGAACCCTATCGATTTCGAGAACTCGGAAGGGAATCTTGGCCTGGCGAATGCGGTG
ATGAATCACCTGGCGGCAAAACTGCCCATTTCGCGCTGGCAGCGGGATTTGACCGATTCCACCGTGCTGCGCAATTTAGG
TGTCGGGATGGGTTATGCACTTATCGCATACCAGTCTACGCTAAAAGGAATCAGTAAATTGGAGGTGAATGAAGCCTATT
TGCTGGCAGAATTGGACAACAATTGGGAAGTCCTGGCTGAACCCATACAGACGGTGATGCGCCGCTACGGAATTGAAAAA
CCGTACGAGAAGCTGAAAGAGCTTACCCGCGGCAAGCGCGTCGATGCTGAGGGTATGCAAGCCTTTATTGATAATCTGCC
GTTGCCGGAAGCGGGGAAAGTACGGCTAAAAACGCTGACGCCAGCTAATTATATCGGCCGCGCCACGACAATGGTTGACG
AATTAAAATAA

Upstream 100 bases:

>100_bases
GTTGGCGGCGGCAGATTTCAGCTTATGTTCGCCCGCAACCGATTGTTCAAAAAAGCTAAACAGATACTTTCACATACTTA
ATCTCCAGGAGTCGCAATCG

Downstream 100 bases:

>100_bases
GCGACGAAAAGGGAACCTGCGCCCGACCCTGGTTCCCTCTCTGTTTATGCGGAGTTTAGTCACTCTCTGGGATACTCTTA
TCCGGTAAGATATTCCTTCC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MELSSLTAVSPIDGRYGDKVSPLRAIFSEFGLLKFRVQVEVRWLQKLAAFDAIKEVPPFDDHANGFLDAIVANFSVEDAE
RIKIIERTTNHDVKAVEYFLKEKVAGQPALQAVSEFIHFACTSEDINNLSHALMLATARESVLLPAWREIIAAVTALAER
YRDIPLLSRTHGQPATPSTMGKEVANVSYRMTRQYRQLERVEILGKINGAVGNYNAHMVAYPEVNWHRFSEEFVTGLGIG
WNPYTTQIEPHDYIAEMFDCIARFNTIVIDFDRDVWGYIALNHFKQRTVAGEIGSSTMPHKVNPIDFENSEGNLGLANAV
MNHLAAKLPISRWQRDLTDSTVLRNLGVGMGYALIAYQSTLKGISKLEVNEAYLLAELDNNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVDAEGMQAFIDNLPLPEAGKVRLKTLTPANYIGRATTMVDELK

Sequences:

>Translated_456_residues
MELSSLTAVSPIDGRYGDKVSPLRAIFSEFGLLKFRVQVEVRWLQKLAAFDAIKEVPPFDDHANGFLDAIVANFSVEDAE
RIKIIERTTNHDVKAVEYFLKEKVAGQPALQAVSEFIHFACTSEDINNLSHALMLATARESVLLPAWREIIAAVTALAER
YRDIPLLSRTHGQPATPSTMGKEVANVSYRMTRQYRQLERVEILGKINGAVGNYNAHMVAYPEVNWHRFSEEFVTGLGIG
WNPYTTQIEPHDYIAEMFDCIARFNTIVIDFDRDVWGYIALNHFKQRTVAGEIGSSTMPHKVNPIDFENSEGNLGLANAV
MNHLAAKLPISRWQRDLTDSTVLRNLGVGMGYALIAYQSTLKGISKLEVNEAYLLAELDNNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVDAEGMQAFIDNLPLPEAGKVRLKTLTPANYIGRATTMVDELK
>Mature_456_residues
MELSSLTAVSPIDGRYGDKVSPLRAIFSEFGLLKFRVQVEVRWLQKLAAFDAIKEVPPFDDHANGFLDAIVANFSVEDAE
RIKIIERTTNHDVKAVEYFLKEKVAGQPALQAVSEFIHFACTSEDINNLSHALMLATARESVLLPAWREIIAAVTALAER
YRDIPLLSRTHGQPATPSTMGKEVANVSYRMTRQYRQLERVEILGKINGAVGNYNAHMVAYPEVNWHRFSEEFVTGLGIG
WNPYTTQIEPHDYIAEMFDCIARFNTIVIDFDRDVWGYIALNHFKQRTVAGEIGSSTMPHKVNPIDFENSEGNLGLANAV
MNHLAAKLPISRWQRDLTDSTVLRNLGVGMGYALIAYQSTLKGISKLEVNEAYLLAELDNNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVDAEGMQAFIDNLPLPEAGKVRLKTLTPANYIGRATTMVDELK

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=456, Percent_Identity=83.9912280701754, Blast_Score=806, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51317; Mature: 51317

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSSLTAVSPIDGRYGDKVSPLRAIFSEFGLLKFRVQVEVRWLQKLAAFDAIKEVPPFD
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCC
DHANGFLDAIVANFSVEDAERIKIIERTTNHDVKAVEYFLKEKVAGQPALQAVSEFIHFA
CCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
CTSEDINNLSHALMLATARESVLLPAWREIIAAVTALAERYRDIPLLSRTHGQPATPSTM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHH
GKEVANVSYRMTRQYRQLERVEILGKINGAVGNYNAHMVAYPEVNWHRFSEEFVTGLGIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCC
WNPYTTQIEPHDYIAEMFDCIARFNTIVIDFDRDVWGYIALNHFKQRTVAGEIGSSTMPH
CCCCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGLANAVMNHLAAKLPISRWQRDLTDSTVLRNLGVGMGYALIAYQST
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LKGISKLEVNEAYLLAELDNNWEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVDAEGMQ
HHHHHHEECCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
AFIDNLPLPEAGKVRLKTLTPANYIGRATTMVDELK
HHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELSSLTAVSPIDGRYGDKVSPLRAIFSEFGLLKFRVQVEVRWLQKLAAFDAIKEVPPFD
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCC
DHANGFLDAIVANFSVEDAERIKIIERTTNHDVKAVEYFLKEKVAGQPALQAVSEFIHFA
CCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
CTSEDINNLSHALMLATARESVLLPAWREIIAAVTALAERYRDIPLLSRTHGQPATPSTM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHH
GKEVANVSYRMTRQYRQLERVEILGKINGAVGNYNAHMVAYPEVNWHRFSEEFVTGLGIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCC
WNPYTTQIEPHDYIAEMFDCIARFNTIVIDFDRDVWGYIALNHFKQRTVAGEIGSSTMPH
CCCCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGLANAVMNHLAAKLPISRWQRDLTDSTVLRNLGVGMGYALIAYQST
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LKGISKLEVNEAYLLAELDNNWEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVDAEGMQ
HHHHHHEECCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
AFIDNLPLPEAGKVRLKTLTPANYIGRATTMVDELK
HHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]