Click here to switch to the map view.

The map label for this gene is metG

Identifier: 85058948

GI number: 85058948

Start: 1599275

End: 1601314

Strand: Reverse

Name: metG

Synonym: SG0970

Alternate gene names: 85058948

Gene position: 1601314-1599275 (Counterclockwise)

Preceding gene: 85058950

Following gene: 85058947

Centisome position: 38.39

GC content: 55.44

Gene sequence:

>2040_bases
ATGACTCAAGTCGCGAAAAAAATATTGGTAACCTGTGCGTTACCTTACGCTAACGGTTCCATCCATCTCGGCCATATGCT
CGAGCATATTCAGGCGGATATTTGGGTCCGCTATCAGCGAATGCGCGGCCATCAGATTTATTTCATTTGTGCTGATGATG
CTCACGGCACGCCAATCATGCTTAAGGCGCAGCAGCTAGGTATTGCGCCGGAAGAGATGATCAATGAAATGAACCTGGAG
CATCAGACGGATTTTGCCGGCTTTGGCATCAGCTACGATAACTATCATTCTACCCACAGCGAAGAGAATCAGCAGCTGTC
GACATTAATTTACCAGCGTCTGAAAGAGAACGGGTTTATCAAATCGCGGACCATTTCCCAGCTGTTTGATCCCGAAAAAG
TCATGTTTTTGCCCGATCGCTTCGTGAAAGGCAGCTGCCCTAAATGCCACTCGCCCGATCAATACGGCGATAACTGCGAA
GTCTGCGGCGCCACCTATAACCCTACGGATCTCATCAATCCAAAATCGGCGGTGTCGGGCACCACGCCGGTCATGCGGGA
ATCGGAACACTTCTTCTTTGATTTACCCGCCTTCAGCGAAATGATGCGCGCCTGGACGCGTTCCGGCGCGCTGCAAGAGC
AGGTGGCGAACAAGATGCAGGAGTGGTTCGAGTCAGGGTTGCAGGAGTGGGATATTTCCCGCGATGCGCCTTACTTCGGT
TTTGAAGTGCCGGATGCGCCGGGCAAATATTTCTATGTCTGGCTGGATGCGCCCATCGGCTATATGAGCGCGTTCCAGAA
CCTGTGCCAGAAGCGCAGCGACCTCCGCTTTGACGAATTTTGGGATGTGGATTCCAAGGCCGATCTTTACCATTTCATCG
GCAAGGACATCGTTTATTTCCACAGCCTGTTCTGGCCGGCGATGCTCGAAGGCAGCCACTTCCGCAAACCGACCAATTTG
TTTGTGCATGGCTATGTGACCGTCAACGGCGCCAAAATGTCCAAATCGCGCGGCACCTTTATTAAAGCTAGCACCTATCT
GGCGCACCTGGATGCAGAGTGCCTGCGCTATTACTATGCCGCTAAACTGTCGTCGCGCATCGATGATATCGACCTTAACC
TCGAAGATTTCATCCAGCGGGTCAATGCCGATATCGTCAATAAAGTCGTCAATTTGGCCTCGCGCAACGCCGGCTTTATC
AATAAACGCTTCGACGGCCAGCTGGCGGCGACGGTGGCCGATCCGGCGCTGTATGCCACTTTCGTTAATGCCGCAACGTC
CATAGGCGATGCGTTCAGCAGCCGCGAAACCAGCCGAGCAATCCGCGAAATCATGGCGCTGGCGGATTTGGCCAATCGCT
ATGTCGATGAGCAAGCGCCCTGGGTCGTGGCGAAACAGGAAGGCCGCGAAGCGGATTTGCAGGCTATCTGTTCAATGGGC
ATCCAGCTGTTCCGGGTGTTGATGACCTATTTGAAACCGGTTCTACCTTCGCTGGCGGAGCGTGCTAAAGCCTTCCTCGC
TACCCCACTTGACTGGGACGACCTGTCAGCCCCGCTGCTGTCGCATCGCATCCGTCCGTTCAAGGCGCTGTTCAGTCGCA
TTGAGGCAGCCCAGGTAGATGCCGTGATCGAGGTATCGCGCCTGGAAGCTGCGGCAGCCGGCGAGCCGGCCGCGGCGCCA
GCGGGCGCGCTGGCCGAGGCCCCTATCGCCCCCACGATAAATTTTGATGATTTCGCCAAGGTGGATATGCGGGTGGCGCT
GATTAAGCGTGCCGAGTCAGTCAAAGGATCGGATAAATTGCTAAAACTCACGCTCGATCTCGGTGGCACCAGCCGTCAGG
TATTCTCCGGTATCCGCGCGGCCTATCCCGATCCACAAGTGTTGGAGGGTCGCCTGACGATCATGGTAGCCAATCTGGCA
CCGCGCAAAATGCGCTTTGGCATATCGGAAGGGATGGTGATGGCAGCCGGCCCCGGCGGCAAGGATATCTTCCTGCTCAG
CCCGGATGCAGGTGCGCTGCCCGGCATGCCGGTAAAATAA

Upstream 100 bases:

>100_bases
TCGCCGCCGTGATGGCAGGCATGCCGCTTGTTTACGTGAGGCATCGCTATCGGTTACCATCGGCTACCCTTATGAAACTT
AACGAAAGCCTATTCCTACC

Downstream 100 bases:

>100_bases
CCCGCGCCGTACGTTCCCTGAGGGCAATGGCCCAAGCCGCCCTCCGGGTATTATCGCCGCTTCCCCTTCGCCGTTTTCTT
ACCGCCCCTGCCAGGGGTCA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 679; Mature: 678

Protein sequence:

>679_residues
MTQVAKKILVTCALPYANGSIHLGHMLEHIQADIWVRYQRMRGHQIYFICADDAHGTPIMLKAQQLGIAPEEMINEMNLE
HQTDFAGFGISYDNYHSTHSEENQQLSTLIYQRLKENGFIKSRTISQLFDPEKVMFLPDRFVKGSCPKCHSPDQYGDNCE
VCGATYNPTDLINPKSAVSGTTPVMRESEHFFFDLPAFSEMMRAWTRSGALQEQVANKMQEWFESGLQEWDISRDAPYFG
FEVPDAPGKYFYVWLDAPIGYMSAFQNLCQKRSDLRFDEFWDVDSKADLYHFIGKDIVYFHSLFWPAMLEGSHFRKPTNL
FVHGYVTVNGAKMSKSRGTFIKASTYLAHLDAECLRYYYAAKLSSRIDDIDLNLEDFIQRVNADIVNKVVNLASRNAGFI
NKRFDGQLAATVADPALYATFVNAATSIGDAFSSRETSRAIREIMALADLANRYVDEQAPWVVAKQEGREADLQAICSMG
IQLFRVLMTYLKPVLPSLAERAKAFLATPLDWDDLSAPLLSHRIRPFKALFSRIEAAQVDAVIEVSRLEAAAAGEPAAAP
AGALAEAPIAPTINFDDFAKVDMRVALIKRAESVKGSDKLLKLTLDLGGTSRQVFSGIRAAYPDPQVLEGRLTIMVANLA
PRKMRFGISEGMVMAAGPGGKDIFLLSPDAGALPGMPVK

Sequences:

>Translated_679_residues
MTQVAKKILVTCALPYANGSIHLGHMLEHIQADIWVRYQRMRGHQIYFICADDAHGTPIMLKAQQLGIAPEEMINEMNLE
HQTDFAGFGISYDNYHSTHSEENQQLSTLIYQRLKENGFIKSRTISQLFDPEKVMFLPDRFVKGSCPKCHSPDQYGDNCE
VCGATYNPTDLINPKSAVSGTTPVMRESEHFFFDLPAFSEMMRAWTRSGALQEQVANKMQEWFESGLQEWDISRDAPYFG
FEVPDAPGKYFYVWLDAPIGYMSAFQNLCQKRSDLRFDEFWDVDSKADLYHFIGKDIVYFHSLFWPAMLEGSHFRKPTNL
FVHGYVTVNGAKMSKSRGTFIKASTYLAHLDAECLRYYYAAKLSSRIDDIDLNLEDFIQRVNADIVNKVVNLASRNAGFI
NKRFDGQLAATVADPALYATFVNAATSIGDAFSSRETSRAIREIMALADLANRYVDEQAPWVVAKQEGREADLQAICSMG
IQLFRVLMTYLKPVLPSLAERAKAFLATPLDWDDLSAPLLSHRIRPFKALFSRIEAAQVDAVIEVSRLEAAAAGEPAAAP
AGALAEAPIAPTINFDDFAKVDMRVALIKRAESVKGSDKLLKLTLDLGGTSRQVFSGIRAAYPDPQVLEGRLTIMVANLA
PRKMRFGISEGMVMAAGPGGKDIFLLSPDAGALPGMPVK
>Mature_678_residues
TQVAKKILVTCALPYANGSIHLGHMLEHIQADIWVRYQRMRGHQIYFICADDAHGTPIMLKAQQLGIAPEEMINEMNLEH
QTDFAGFGISYDNYHSTHSEENQQLSTLIYQRLKENGFIKSRTISQLFDPEKVMFLPDRFVKGSCPKCHSPDQYGDNCEV
CGATYNPTDLINPKSAVSGTTPVMRESEHFFFDLPAFSEMMRAWTRSGALQEQVANKMQEWFESGLQEWDISRDAPYFGF
EVPDAPGKYFYVWLDAPIGYMSAFQNLCQKRSDLRFDEFWDVDSKADLYHFIGKDIVYFHSLFWPAMLEGSHFRKPTNLF
VHGYVTVNGAKMSKSRGTFIKASTYLAHLDAECLRYYYAAKLSSRIDDIDLNLEDFIQRVNADIVNKVVNLASRNAGFIN
KRFDGQLAATVADPALYATFVNAATSIGDAFSSRETSRAIREIMALADLANRYVDEQAPWVVAKQEGREADLQAICSMGI
QLFRVLMTYLKPVLPSLAERAKAFLATPLDWDDLSAPLLSHRIRPFKALFSRIEAAQVDAVIEVSRLEAAAAGEPAAAPA
GALAEAPIAPTINFDDFAKVDMRVALIKRAESVKGSDKLLKLTLDLGGTSRQVFSGIRAAYPDPQVLEGRLTIMVANLAP
RKMRFGISEGMVMAAGPGGKDIFLLSPDAGALPGMPVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=588, Percent_Identity=27.3809523809524, Blast_Score=243, Evalue=4e-64,
Organism=Homo sapiens, GI24308436, Length=513, Percent_Identity=24.3664717348928, Blast_Score=158, Evalue=2e-38,
Organism=Escherichia coli, GI1788432, Length=680, Percent_Identity=81.3235294117647, Blast_Score=1174, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=693, Percent_Identity=25.8297258297258, Blast_Score=241, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI212645096, Length=528, Percent_Identity=24.0530303030303, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71992781, Length=422, Percent_Identity=24.4075829383886, Blast_Score=112, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6321704, Length=555, Percent_Identity=27.3873873873874, Blast_Score=245, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6321610, Length=542, Percent_Identity=21.2177121771218, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24655311, Length=572, Percent_Identity=28.8461538461538, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI21358483, Length=523, Percent_Identity=24.4741873804971, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_SODGM (Q2NUD0)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_454650.1
- ProteinModelPortal:   Q2NUD0
- STRING:   Q2NUD0
- GeneID:   3867097
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG0970
- NMPDR:   fig|343509.6.peg.2019
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- PhylomeDB:   Q2NUD0
- ProtClustDB:   PRK00133
- BioCyc:   SGLO343509:SG0970-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 75725; Mature: 75594

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 335-335

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQVAKKILVTCALPYANGSIHLGHMLEHIQADIWVRYQRMRGHQIYFICADDAHGTPIM
CCHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEE
LKAQQLGIAPEEMINEMNLEHQTDFAGFGISYDNYHSTHSEENQQLSTLIYQRLKENGFI
EEHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCE
KSRTISQLFDPEKVMFLPDRFVKGSCPKCHSPDQYGDNCEVCGATYNPTDLINPKSAVSG
EHHHHHHHCCCCEEEECCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCC
TTPVMRESEHFFFDLPAFSEMMRAWTRSGALQEQVANKMQEWFESGLQEWDISRDAPYFG
CCCCCCCCCCEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FEVPDAPGKYFYVWLDAPIGYMSAFQNLCQKRSDLRFDEFWDVDSKADLYHFIGKDIVYF
EECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHH
HSLFWPAMLEGSHFRKPTNLFVHGYVTVNGAKMSKSRGTFIKASTYLAHLDAECLRYYYA
HHHHHHHHHCCCCCCCCCCEEEEEEEEEECCEECCCCCCEEEHHHHHHHHHHHHHHHHHH
AKLSSRIDDIDLNLEDFIQRVNADIVNKVVNLASRNAGFINKRFDGQLAATVADPALYAT
HHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHCHHHHHH
FVNAATSIGDAFSSRETSRAIREIMALADLANRYVDEQAPWVVAKQEGREADLQAICSMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHH
IQLFRVLMTYLKPVLPSLAERAKAFLATPLDWDDLSAPLLSHRIRPFKALFSRIEAAQVD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHCCCHHHHHHHHHHHHHHH
AVIEVSRLEAAAAGEPAAAPAGALAEAPIAPTINFDDFAKVDMRVALIKRAESVKGSDKL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEE
LKLTLDLGGTSRQVFSGIRAAYPDPQVLEGRLTIMVANLAPRKMRFGISEGMVMAAGPGG
EEEEEECCCCHHHHHHHHHHCCCCCHHCCCEEEEEEECCCCHHHHCCCCCCEEEEECCCC
KDIFLLSPDAGALPGMPVK
CEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
TQVAKKILVTCALPYANGSIHLGHMLEHIQADIWVRYQRMRGHQIYFICADDAHGTPIM
CHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEE
LKAQQLGIAPEEMINEMNLEHQTDFAGFGISYDNYHSTHSEENQQLSTLIYQRLKENGFI
EEHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCE
KSRTISQLFDPEKVMFLPDRFVKGSCPKCHSPDQYGDNCEVCGATYNPTDLINPKSAVSG
EHHHHHHHCCCCEEEECCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCC
TTPVMRESEHFFFDLPAFSEMMRAWTRSGALQEQVANKMQEWFESGLQEWDISRDAPYFG
CCCCCCCCCCEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FEVPDAPGKYFYVWLDAPIGYMSAFQNLCQKRSDLRFDEFWDVDSKADLYHFIGKDIVYF
EECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHH
HSLFWPAMLEGSHFRKPTNLFVHGYVTVNGAKMSKSRGTFIKASTYLAHLDAECLRYYYA
HHHHHHHHHCCCCCCCCCCEEEEEEEEEECCEECCCCCCEEEHHHHHHHHHHHHHHHHHH
AKLSSRIDDIDLNLEDFIQRVNADIVNKVVNLASRNAGFINKRFDGQLAATVADPALYAT
HHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHCHHHHHH
FVNAATSIGDAFSSRETSRAIREIMALADLANRYVDEQAPWVVAKQEGREADLQAICSMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHH
IQLFRVLMTYLKPVLPSLAERAKAFLATPLDWDDLSAPLLSHRIRPFKALFSRIEAAQVD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHCCCHHHHHHHHHHHHHHH
AVIEVSRLEAAAAGEPAAAPAGALAEAPIAPTINFDDFAKVDMRVALIKRAESVKGSDKL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEE
LKLTLDLGGTSRQVFSGIRAAYPDPQVLEGRLTIMVANLAPRKMRFGISEGMVMAAGPGG
EEEEEECCCCHHHHHHHHHHCCCCCHHCCCEEEEEEECCCCHHHHCCCCCCEEEEECCCC
KDIFLLSPDAGALPGMPVK
CEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA